Click here to switch to the map view.

The map label for this gene is cobB [H]

Identifier: 126696516

GI number: 126696516

Start: 990459

End: 991838

Strand: Reverse

Name: cobB [H]

Synonym: P9301_11781

Alternate gene names: 126696516

Gene position: 991838-990459 (Counterclockwise)

Preceding gene: 126696518

Following gene: 126696512

Centisome position: 60.41

GC content: 30.72

Gene sequence:

>1380_bases
ATGCCTTGTGTAATATCTTCTCCTTCAACTGATAGTGGGAAAACTACATTATCCCTTTTGATATCTTGTTGGGCGTTTTC
AAAAGGTATAAAGATACAAACTTTCAAGGTTGGCCCAGATTATCTTGATCAACAACAACTTAGCTCCATTGGCCAACCTA
TTTGTAGAAATTTAGATATTTTTTTAAATGGTGAGGAATGGGTTCAAGAAAGTTTTTTTAAACATTCTTTGAAATATGAA
TTCTCATTAATTGAAGGAGCAATGGGTCTATTTGATGGGTTAGGAGCAACAACCTATTCAAGCACAGCAAATATATCTAA
ACTTCTCAATGCTCCAGTAATTTTTATTGTTAATGCTAGAGGTCAAGTAGCTTCTCTTTTAGCGACTGTTAGAGGTTTTA
AAGATTTTGATAGTGAATTGTCAATAGCAGGGATTATTTTCAATAACGTTAATTCTGATAGACATAAAAAATTAATTGAA
GAAGTTTTTAAAAATGAAGATATCGAAATTCTTGGTTTTTTGCCGTCCGATTCAAAAATAACTTTAAACAAATCTAATTT
AGGTTTAATTTCTCCATTGGATAATGGGAAACAAATTGATATTGAATATTTTGCTAATTATGCGGAAAGAAATCTTGATT
TATTTTCTCTTATTAAATTCCTGAAATCTCCTCAAAAGAAAATTTTTAATTCTGCTAGTTTTGAAGACTTTAAAATTGAA
AAAAGTAAACCTATTGCAATTGCAGAAGATAAAATCTTTCATTTTCAATACCCCGAAACTAAGGAGTTTTTGAGTGACGT
AGGTATTCCATTGATTTCATGGAGTATTTATGATGATGAAGAGATACCCAACGAGGCTTCTTCTTTAATTATTCCTGGGG
GGTTCCCTGAAAAATATGCTGAGCATATAAGTAACTCTAAAAAAAGCTTAAATTCGTTAAGGAAATTCCGCGAAAATGGA
TTTATATATGCTGAGTGCGGAGGGATGATGATTTTAGGAGACTTTATAAAAGATGAAAGTGGTAATAATCATAAAATGAG
TGGGATCCTTCCTTTTAGATCAAAAAAAAGTAAGCTCACAGTAGGTTATAGATATATTGAAGGTCTAAAAGACACTCCGA
TCATTAGACAAAATCAATTAATTAGAGGACATGAATTTCATTATTGGGAAATTGAAAATAATTTATCTGAACTTGATTTA
AGAAAAGCTGAGCATCATATGAAACTTTCTTCCCCATGGAAAATTAAATCTTGGGAAACTGAATATAAAAATGAAGGTTT
TTTTGATCAAAAATTGCATGCAAGTTGGATTCACTTACATTTGCCAAGTTCTCCAAAAGTCGCAAAAAACTTTATAGATG
CAACCCAAATTAGTTTTTAA

Upstream 100 bases:

>100_bases
TAGTAAATTATTGGGTAGTAGTAGAGGTAATACAAGCCCTCTTTTTGAAAATTCTATTAAAATAGCTCTTCAAATATTTA
ATGGTTTTAAAAACTAATAA

Downstream 100 bases:

>100_bases
AAGGGTTCTTAATTTATTATCAAATCAAATATTTTTAGAGGTGAACCTAAAAAAAACATACCAGGCAAAGTAATTAATGG
TCCTAAAAAACTTCCCACTA

Product: putative cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MPCVISSPSTDSGKTTLSLLISCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDIFLNGEEWVQESFFKHSLKYE
FSLIEGAMGLFDGLGATTYSSTANISKLLNAPVIFIVNARGQVASLLATVRGFKDFDSELSIAGIIFNNVNSDRHKKLIE
EVFKNEDIEILGFLPSDSKITLNKSNLGLISPLDNGKQIDIEYFANYAERNLDLFSLIKFLKSPQKKIFNSASFEDFKIE
KSKPIAIAEDKIFHFQYPETKEFLSDVGIPLISWSIYDDEEIPNEASSLIIPGGFPEKYAEHISNSKKSLNSLRKFRENG
FIYAECGGMMILGDFIKDESGNNHKMSGILPFRSKKSKLTVGYRYIEGLKDTPIIRQNQLIRGHEFHYWEIENNLSELDL
RKAEHHMKLSSPWKIKSWETEYKNEGFFDQKLHASWIHLHLPSSPKVAKNFIDATQISF

Sequences:

>Translated_459_residues
MPCVISSPSTDSGKTTLSLLISCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDIFLNGEEWVQESFFKHSLKYE
FSLIEGAMGLFDGLGATTYSSTANISKLLNAPVIFIVNARGQVASLLATVRGFKDFDSELSIAGIIFNNVNSDRHKKLIE
EVFKNEDIEILGFLPSDSKITLNKSNLGLISPLDNGKQIDIEYFANYAERNLDLFSLIKFLKSPQKKIFNSASFEDFKIE
KSKPIAIAEDKIFHFQYPETKEFLSDVGIPLISWSIYDDEEIPNEASSLIIPGGFPEKYAEHISNSKKSLNSLRKFRENG
FIYAECGGMMILGDFIKDESGNNHKMSGILPFRSKKSKLTVGYRYIEGLKDTPIIRQNQLIRGHEFHYWEIENNLSELDL
RKAEHHMKLSSPWKIKSWETEYKNEGFFDQKLHASWIHLHLPSSPKVAKNFIDATQISF
>Mature_458_residues
PCVISSPSTDSGKTTLSLLISCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDIFLNGEEWVQESFFKHSLKYEF
SLIEGAMGLFDGLGATTYSSTANISKLLNAPVIFIVNARGQVASLLATVRGFKDFDSELSIAGIIFNNVNSDRHKKLIEE
VFKNEDIEILGFLPSDSKITLNKSNLGLISPLDNGKQIDIEYFANYAERNLDLFSLIKFLKSPQKKIFNSASFEDFKIEK
SKPIAIAEDKIFHFQYPETKEFLSDVGIPLISWSIYDDEEIPNEASSLIIPGGFPEKYAEHISNSKKSLNSLRKFRENGF
IYAECGGMMILGDFIKDESGNNHKMSGILPFRSKKSKLTVGYRYIEGLKDTPIIRQNQLIRGHEFHYWEIENNLSELDLR
KAEHHMKLSSPWKIKSWETEYKNEGFFDQKLHASWIHLHLPSSPKVAKNFIDATQISF

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 52145; Mature: 52014

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCVISSPSTDSGKTTLSLLISCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDI
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCHHHHCCCEE
FLNGEEWVQESFFKHSLKYEFSLIEGAMGLFDGLGATTYSSTANISKLLNAPVIFIVNAR
EECCHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEECCC
GQVASLLATVRGFKDFDSELSIAGIIFNNVNSDRHKKLIEEVFKNEDIEILGFLPSDSKI
CHHHHHHHHHHCHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCEE
TLNKSNLGLISPLDNGKQIDIEYFANYAERNLDLFSLIKFLKSPQKKIFNSASFEDFKIE
EEECCCCEEEEECCCCCEEEHHHHHHHHHCCCCHHHHHHHHHCHHHHHHCCCCCCCEEEC
KSKPIAIAEDKIFHFQYPETKEFLSDVGIPLISWSIYDDEEIPNEASSLIIPGGFPEKYA
CCCCEEEECCCEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEECCCCHHHHH
EHISNSKKSLNSLRKFRENGFIYAECGGMMILGDFIKDESGNNHKMSGILPFRSKKSKLT
HHHCCHHHHHHHHHHHHHCCEEEEECCCEEEEEHHHCCCCCCCCEEEEEECCCCCCCEEE
VGYRYIEGLKDTPIIRQNQLIRGHEFHYWEIENNLSELDLRKAEHHMKLSSPWKIKSWET
EEHHHHCCCCCCCCCCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEECCCCC
EYKNEGFFDQKLHASWIHLHLPSSPKVAKNFIDATQISF
CCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PCVISSPSTDSGKTTLSLLISCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDI
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCHHHHCCCEE
FLNGEEWVQESFFKHSLKYEFSLIEGAMGLFDGLGATTYSSTANISKLLNAPVIFIVNAR
EECCHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEECCC
GQVASLLATVRGFKDFDSELSIAGIIFNNVNSDRHKKLIEEVFKNEDIEILGFLPSDSKI
CHHHHHHHHHHCHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCEE
TLNKSNLGLISPLDNGKQIDIEYFANYAERNLDLFSLIKFLKSPQKKIFNSASFEDFKIE
EEECCCCEEEEECCCCCEEEHHHHHHHHHCCCCHHHHHHHHHCHHHHHHCCCCCCCEEEC
KSKPIAIAEDKIFHFQYPETKEFLSDVGIPLISWSIYDDEEIPNEASSLIIPGGFPEKYA
CCCCEEEECCCEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEECCCCHHHHH
EHISNSKKSLNSLRKFRENGFIYAECGGMMILGDFIKDESGNNHKMSGILPFRSKKSKLT
HHHCCHHHHHHHHHHHHHCCEEEEECCCEEEEEHHHCCCCCCCCEEEEEECCCCCCCEEE
VGYRYIEGLKDTPIIRQNQLIRGHEFHYWEIENNLSELDLRKAEHHMKLSSPWKIKSWET
EEHHHHCCCCCCCCCCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEECCCCC
EYKNEGFFDQKLHASWIHLHLPSSPKVAKNFIDATQISF
CCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA