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The map label for this gene is nth [H]

Identifier: 126696201

GI number: 126696201

Start: 748129

End: 748782

Strand: Reverse

Name: nth [H]

Synonym: P9301_08631

Alternate gene names: 126696201

Gene position: 748782-748129 (Counterclockwise)

Preceding gene: 126696204

Following gene: 126696200

Centisome position: 45.61

GC content: 33.49

Gene sequence:

>654_bases
ATGAGAAAGTCTGAAAGAGCAGAAATAATACGCAAGGAACTTAAAAAGTTATATCCATCGCCTCCAATACCCCTTGATCA
TACAAATGCATATACACTTCTAGTCGCCGTAGTTTTAAGTGCTCAATCAACAGATAAGAAAGTTAATGAATTAACAAAAA
GGTTATTTAAGGTTGCAGATAATCCAGAAAAGATGATCCAGCTAGGTATTAATGGCATTTACGAATACATAAAATTTTTA
GGTCTATCTAATCAAAAATCAAAGAACATCTTTAACTTATCTAAACTATTGATTGAAAAGCATAATGGTAAGGTCCCAAA
TTCTTTTGAGAAACTTGAATCTCTTCCAGGGGTAGGTCATAAAACAGCCTCAGTTGTAATGTCTCAAGTGTTTAAAATAC
CCTCGTTCCCTGTCGATACTCACATACACAGGTTGTCACAAAGATGGGGGCTATCAAATGGAGATAGCGTAGTTCAAACA
GAAAAAGACCTAAAAAAAATATTTCCTGTTAATGATTGGAATACCTTACATTTGCAAATAATCTTTTATGGCAGAGAATA
TTGCACAGCAAGAGGCTGTGATGGAACAAAATGTTATTTGTGTCGCACTCTTTATCCCAAAAGAAAAAAGAAATTTATAT
GTAAAAAGCCTTAA

Upstream 100 bases:

>100_bases
TTCACTAAGTTATTCTTATTTAAATATATTAAGTTAGCAGAAAATATTCACCTAAAATATCCCTTGTATAATTTTATACA
CCATTTAAACTTCAAATTTA

Downstream 100 bases:

>100_bases
GAAATTTATATAATATTTAAATTAGTTTTTTTAACCATGAAAATTGCAATTACTGGTGCATCGGGCAAAACAGGATATAG
AATCTCCGAAGAAGCAGTTA

Product: putative endonuclease

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRKSERAEIIRKELKKLYPSPPIPLDHTNAYTLLVAVVLSAQSTDKKVNELTKRLFKVADNPEKMIQLGINGIYEYIKFL
GLSNQKSKNIFNLSKLLIEKHNGKVPNSFEKLESLPGVGHKTASVVMSQVFKIPSFPVDTHIHRLSQRWGLSNGDSVVQT
EKDLKKIFPVNDWNTLHLQIIFYGREYCTARGCDGTKCYLCRTLYPKRKKKFICKKP

Sequences:

>Translated_217_residues
MRKSERAEIIRKELKKLYPSPPIPLDHTNAYTLLVAVVLSAQSTDKKVNELTKRLFKVADNPEKMIQLGINGIYEYIKFL
GLSNQKSKNIFNLSKLLIEKHNGKVPNSFEKLESLPGVGHKTASVVMSQVFKIPSFPVDTHIHRLSQRWGLSNGDSVVQT
EKDLKKIFPVNDWNTLHLQIIFYGREYCTARGCDGTKCYLCRTLYPKRKKKFICKKP
>Mature_217_residues
MRKSERAEIIRKELKKLYPSPPIPLDHTNAYTLLVAVVLSAQSTDKKVNELTKRLFKVADNPEKMIQLGINGIYEYIKFL
GLSNQKSKNIFNLSKLLIEKHNGKVPNSFEKLESLPGVGHKTASVVMSQVFKIPSFPVDTHIHRLSQRWGLSNGDSVVQT
EKDLKKIFPVNDWNTLHLQIIFYGREYCTARGCDGTKCYLCRTLYPKRKKKFICKKP

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=161, Percent_Identity=26.7080745341615, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787920, Length=191, Percent_Identity=42.4083769633508, Blast_Score=161, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17554540, Length=214, Percent_Identity=26.6355140186916, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324530, Length=182, Percent_Identity=24.1758241758242, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550361, Length=190, Percent_Identity=26.8421052631579, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24908; Mature: 24908

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSERAEIIRKELKKLYPSPPIPLDHTNAYTLLVAVVLSAQSTDKKVNELTKRLFKVAD
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
NPEKMIQLGINGIYEYIKFLGLSNQKSKNIFNLSKLLIEKHNGKVPNSFEKLESLPGVGH
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCH
KTASVVMSQVFKIPSFPVDTHIHRLSQRWGLSNGDSVVQTEKDLKKIFPVNDWNTLHLQI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
IFYGREYCTARGCDGTKCYLCRTLYPKRKKKFICKKP
EEECCHHHHCCCCCCCCEEEEHHHCCHHHHCCCCCCC
>Mature Secondary Structure
MRKSERAEIIRKELKKLYPSPPIPLDHTNAYTLLVAVVLSAQSTDKKVNELTKRLFKVAD
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
NPEKMIQLGINGIYEYIKFLGLSNQKSKNIFNLSKLLIEKHNGKVPNSFEKLESLPGVGH
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCH
KTASVVMSQVFKIPSFPVDTHIHRLSQRWGLSNGDSVVQTEKDLKKIFPVNDWNTLHLQI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
IFYGREYCTARGCDGTKCYLCRTLYPKRKKKFICKKP
EEECCHHHHCCCCCCCCEEEEHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]