Click here to switch to the map view.

The map label for this gene is epsE [H]

Identifier: 126696029

GI number: 126696029

Start: 613877

End: 615583

Strand: Reverse

Name: epsE [H]

Synonym: P9301_06911

Alternate gene names: 126696029

Gene position: 615583-613877 (Counterclockwise)

Preceding gene: 126696035

Following gene: 126696028

Centisome position: 37.49

GC content: 32.28

Gene sequence:

>1707_bases
ATGATTGATACTAATGAAATAAGTAAATTTCTCACGCCTGAATTATGTGAAGATGCAGGGATAATAGTTATTGAAATTGG
CGAGGAAACTTTAGATTTAGGAGCAATGAATTTAGATTATATTAAAGTAAAAAAAGTTATCAAAACTATTGAAAGTGAAT
ATAATTTAAAAGTTTCCTTAAAACAAATTACATCTTTAGAATGGGAAACTTGGTTTGAGAATAATCATGACACATCAGTT
GAAAAAATTCAAAAAAGTTCAAATCAAAATCAAACTTTAGAAAAAATTAATGATGTAGAAGAAAATGTACTTAATGATGA
ATATATCAATTATTTTGAAGAGTCAAACACAGAGAGTATTCAATACAATATTCAAAACTCAAATGAATCCTCTATTAATG
AGGACATAGAACTAATTGATGATGCTAATGATGAAGCACTTATTAAGTTGGCTCAGGCAAATATTGGTGACGATTCAGAA
ATGGATAATGATATTTTATTTGGGGAAGAACTTACTGCATCTAATGATCCCGTAATTGCCGGTGTAGCCTCAATTTTAAG
TAAATGTTTCACATTAAAGGGATCAGATATTCATGCAGAACCTTTAGAGGATCGCTTAAGGATAAGATATAGAATTGATG
GAGTTTTAAAAGAGGCTTTTGCGTTTCCAAAATCACATGTAAACCCAATAATTAGTAGGATTAAGATAATGAGTAAGCTA
GATATATCTGAAAAAAGGTTACCCCAAGATGGGAGAATAAGATGTCTGTTAAGAGGAAGAAGATCTGACTTTAGAGTAAG
CACATTGCCTGGAAGATGGGGAGAAAAAGTTGTGCTTAGGGCACTTGAGAGTGATAATTCTGTACTTAACCTTTCAAAAT
TAATTACAGAAAATGATGAATTAAGTTTAATAAAAAATATGTCTAAATCTCCATATGGAATTGTGATTGTAGTTGGACCG
ACCGGTAGCGGCAAATCAACAACTTTATATTCTATGTTGAGTGACCTTAATAAACCAGGAGTAAATATAAGTACGGTTGA
AGACCCAGTAGAATATACACTTGATGGCATTCATCAAGTTCAAGTAATTAGAGAAAAGGGACTTGATTTTTCGAGAGCTT
TAAGGTCTTTAATGAGGCAAGACCCAGATATTATTTTAGTGGGAGAAACTAGAGATAAAGAAACAGCTCAAGCCGCAATG
GAAGCGGCATTAACTGGTCACATGGTTTTCACAACGTTGCATGCTAATGATACTGCAACAGCAATTACAAGACTTGCTGA
AATGGAAATCCCACCTTATCTTATTGGGGCCTCAATCATAGGAGTTGTTGCTCAAAGATTAGTTAGAAAAGTTTGCAAAT
CTTGTAGTGAATTAAAAAAAATAAACGCACATGAAAATCATTTAGCATTTTCTTTAGGAGTAGAAAAAGCAAGATTCTTA
AACAAAACAAATAAAGGCTGCCCCGTATGCAACGGAAGTGGCTATAAAGGAAGAGTTGGTATCTATGAAGTAATGAAAGT
TAATGACTCGATTAGAGATTTAATAATGAAGCAATCCAATGCAGATCAAATAAGAGAACGTGCTTTTGAAAAAAATGGAA
GAAGTTTACTAAAATATGGTATGGATTTAGTAAGAAATGAACTTACTACAATTGAAGAAGTTGAACGTGTTTGCTTACTC
AATGACACTCAATCTGAAGAATTATGA

Upstream 100 bases:

>100_bases
AATAAAGTACTATTAATCCTTATTATATGTCTATATTTAAATTAGGATTTCTTTGTATTAAATTTTTTCAGCTCTCAAAA
ACAATTCATAGTGAAAAAAT

Downstream 100 bases:

>100_bases
AATTAACTAATCTCATGTCAGAGTTAGTAAAAAGAAATGGTTCTGACCTGCATTTAACAGGAGACAGCTATCCTTTTTTT
AGAATTCAAGGACAAATTTT

Product: Type II secretory pathway ATPase PulE/Tfp pilus assembly pathway ATPase PilB-like protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MIDTNEISKFLTPELCEDAGIIVIEIGEETLDLGAMNLDYIKVKKVIKTIESEYNLKVSLKQITSLEWETWFENNHDTSV
EKIQKSSNQNQTLEKINDVEENVLNDEYINYFEESNTESIQYNIQNSNESSINEDIELIDDANDEALIKLAQANIGDDSE
MDNDILFGEELTASNDPVIAGVASILSKCFTLKGSDIHAEPLEDRLRIRYRIDGVLKEAFAFPKSHVNPIISRIKIMSKL
DISEKRLPQDGRIRCLLRGRRSDFRVSTLPGRWGEKVVLRALESDNSVLNLSKLITENDELSLIKNMSKSPYGIVIVVGP
TGSGKSTTLYSMLSDLNKPGVNISTVEDPVEYTLDGIHQVQVIREKGLDFSRALRSLMRQDPDIILVGETRDKETAQAAM
EAALTGHMVFTTLHANDTATAITRLAEMEIPPYLIGASIIGVVAQRLVRKVCKSCSELKKINAHENHLAFSLGVEKARFL
NKTNKGCPVCNGSGYKGRVGIYEVMKVNDSIRDLIMKQSNADQIRERAFEKNGRSLLKYGMDLVRNELTTIEEVERVCLL
NDTQSEEL

Sequences:

>Translated_568_residues
MIDTNEISKFLTPELCEDAGIIVIEIGEETLDLGAMNLDYIKVKKVIKTIESEYNLKVSLKQITSLEWETWFENNHDTSV
EKIQKSSNQNQTLEKINDVEENVLNDEYINYFEESNTESIQYNIQNSNESSINEDIELIDDANDEALIKLAQANIGDDSE
MDNDILFGEELTASNDPVIAGVASILSKCFTLKGSDIHAEPLEDRLRIRYRIDGVLKEAFAFPKSHVNPIISRIKIMSKL
DISEKRLPQDGRIRCLLRGRRSDFRVSTLPGRWGEKVVLRALESDNSVLNLSKLITENDELSLIKNMSKSPYGIVIVVGP
TGSGKSTTLYSMLSDLNKPGVNISTVEDPVEYTLDGIHQVQVIREKGLDFSRALRSLMRQDPDIILVGETRDKETAQAAM
EAALTGHMVFTTLHANDTATAITRLAEMEIPPYLIGASIIGVVAQRLVRKVCKSCSELKKINAHENHLAFSLGVEKARFL
NKTNKGCPVCNGSGYKGRVGIYEVMKVNDSIRDLIMKQSNADQIRERAFEKNGRSLLKYGMDLVRNELTTIEEVERVCLL
NDTQSEEL
>Mature_568_residues
MIDTNEISKFLTPELCEDAGIIVIEIGEETLDLGAMNLDYIKVKKVIKTIESEYNLKVSLKQITSLEWETWFENNHDTSV
EKIQKSSNQNQTLEKINDVEENVLNDEYINYFEESNTESIQYNIQNSNESSINEDIELIDDANDEALIKLAQANIGDDSE
MDNDILFGEELTASNDPVIAGVASILSKCFTLKGSDIHAEPLEDRLRIRYRIDGVLKEAFAFPKSHVNPIISRIKIMSKL
DISEKRLPQDGRIRCLLRGRRSDFRVSTLPGRWGEKVVLRALESDNSVLNLSKLITENDELSLIKNMSKSPYGIVIVVGP
TGSGKSTTLYSMLSDLNKPGVNISTVEDPVEYTLDGIHQVQVIREKGLDFSRALRSLMRQDPDIILVGETRDKETAQAAM
EAALTGHMVFTTLHANDTATAITRLAEMEIPPYLIGASIIGVVAQRLVRKVCKSCSELKKINAHENHLAFSLGVEKARFL
NKTNKGCPVCNGSGYKGRVGIYEVMKVNDSIRDLIMKQSNADQIRERAFEKNGRSLLKYGMDLVRNELTTIEEVERVCLL
NDTQSEEL

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=417, Percent_Identity=43.4052757793765, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI1786296, Length=372, Percent_Identity=40.0537634408602, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI87082188, Length=168, Percent_Identity=39.8809523809524, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 63724; Mature: 63724

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00014 ER_TARGET

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTNEISKFLTPELCEDAGIIVIEIGEETLDLGAMNLDYIKVKKVIKTIESEYNLKVSL
CCCHHHHHHHCCHHHHCCCCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEH
KQITSLEWETWFENNHDTSVEKIQKSSNQNQTLEKINDVEENVLNDEYINYFEESNTESI
HHHHCCCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEE
QYNIQNSNESSINEDIELIDDANDEALIKLAQANIGDDSEMDNDILFGEELTASNDPVIA
EEEECCCCCCCCCCCHHHHCCCCCHHHHEEHHCCCCCCCCCCCCEEECCCCCCCCCCHHH
GVASILSKCFTLKGSDIHAEPLEDRLRIRYRIDGVLKEAFAFPKSHVNPIISRIKIMSKL
HHHHHHHHHHCCCCCCCCCCCCHHHHEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
DISEKRLPQDGRIRCLLRGRRSDFRVSTLPGRWGEKVVLRALESDNSVLNLSKLITENDE
CCHHHHCCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHCCCCEEHHHHHHCCCCH
LSLIKNMSKSPYGIVIVVGPTGSGKSTTLYSMLSDLNKPGVNISTVEDPVEYTLDGIHQV
HHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHH
QVIREKGLDFSRALRSLMRQDPDIILVGETRDKETAQAAMEAALTGHMVFTTLHANDTAT
HHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECCCHHH
AITRLAEMEIPPYLIGASIIGVVAQRLVRKVCKSCSELKKINAHENHLAFSLGVEKARFL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHH
NKTNKGCPVCNGSGYKGRVGIYEVMKVNDSIRDLIMKQSNADQIRERAFEKNGRSLLKYG
HCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHH
MDLVRNELTTIEEVERVCLLNDTQSEEL
HHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIDTNEISKFLTPELCEDAGIIVIEIGEETLDLGAMNLDYIKVKKVIKTIESEYNLKVSL
CCCHHHHHHHCCHHHHCCCCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEH
KQITSLEWETWFENNHDTSVEKIQKSSNQNQTLEKINDVEENVLNDEYINYFEESNTESI
HHHHCCCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEE
QYNIQNSNESSINEDIELIDDANDEALIKLAQANIGDDSEMDNDILFGEELTASNDPVIA
EEEECCCCCCCCCCCHHHHCCCCCHHHHEEHHCCCCCCCCCCCCEEECCCCCCCCCCHHH
GVASILSKCFTLKGSDIHAEPLEDRLRIRYRIDGVLKEAFAFPKSHVNPIISRIKIMSKL
HHHHHHHHHHCCCCCCCCCCCCHHHHEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
DISEKRLPQDGRIRCLLRGRRSDFRVSTLPGRWGEKVVLRALESDNSVLNLSKLITENDE
CCHHHHCCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHCCCCEEHHHHHHCCCCH
LSLIKNMSKSPYGIVIVVGPTGSGKSTTLYSMLSDLNKPGVNISTVEDPVEYTLDGIHQV
HHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHH
QVIREKGLDFSRALRSLMRQDPDIILVGETRDKETAQAAMEAALTGHMVFTTLHANDTAT
HHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECCCHHH
AITRLAEMEIPPYLIGASIIGVVAQRLVRKVCKSCSELKKINAHENHLAFSLGVEKARFL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHH
NKTNKGCPVCNGSGYKGRVGIYEVMKVNDSIRDLIMKQSNADQIRERAFEKNGRSLLKYG
HCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHH
MDLVRNELTTIEEVERVCLLNDTQSEEL
HHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]