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The map label for this gene is fumB [H]

Identifier: 126447924

GI number: 126447924

Start: 1994982

End: 1996517

Strand: Direct

Name: fumB [H]

Synonym: BMA10247_A2058

Alternate gene names: 126447924

Gene position: 1994982-1996517 (Clockwise)

Preceding gene: 126447234

Following gene: 126445639

Centisome position: 84.8

GC content: 65.89

Gene sequence:

>1536_bases
ATGACCGTCATCAAACAGGAAGACCTGATCCAGAGCATCGCGGATTCGCTGCAGTACATCAGCTACTACCATCCGCTCGA
TTACATCCAGGCGCTCGGCCGCGCATACGAGCTCGAGCAGAGCCCGGCCGCGAAGGATGCGATCGCGCAGATCCTGACGA
ACAGCCGCATGTGCGCGGAAGGCAAGCGCCCGATCTGCCAGGACACGGGCATCGTCACGATCTTCGTGAAGGTCGGCATG
GACGTGCGCTGGGCAAGCGAGAACGGCGCCGCGACGATGAGCGTCACCGACATGATCAACGAAGGCGTGCGCCGCGGCTA
CACGCATCCGGACAACGTGCTGCGCGCATCGATCGTCAACCCGCCCGAAGGCGCGCGCAAGAACACGAAGGACAACACGC
CGGCCGTGATCCATTACGAGATCGTGCCGGGCGACAAGGTCGACGTGCAAGTCGCGGCGAAGGGCGGCGGCTCCGAGAAC
AAATCGAAGTTCGTGATGCTGAACCCGTCGGATTCGATCGTCGACTGGGTACTGAAGACCGTGCCGACGATGGGCGCGGG
CTGGTGCCCGCCGGGCATGCTCGGCATCGGCATCGGCGGCACCGCCGAGAAGGCGATGCTGATGGCGAAGGAATCGCTGA
TGGAGCCGATCGACATCCAGGAAATCATCGCGCGCGGCCCGAAGGACTGGGTCGAGGAACTGCGCGTCGAGCTGCACGAG
AAGGTCAACGCGCTCGGCATCGGCGCGCAGGGTCTCGGCGGCCTCGCGACCGTGCTCGACGTGAAGATCCTGGCCGCGCC
GACGCACGCGGCGTCGAAGCCCGTCGCGATGATCCCGAACTGCGCGGCCACGCGCCATGCGCACTTCGTGCTCGACGGCT
CGGGCGCGGCGAAGCTCGAGGCGCCGTCGCTCGACGCATGGCCGAAGGTTCAATGGGAACCGAACACCGAGACGAGCAAG
CGCGTCGACCTGAACACGCTGACGCCGGAAGAAGTCGCATCGTGGAAGCCGGGCCAGACGCTGCTGCTGTCGGGCAAGAT
GCTCACGGGCCGCGACGCCGCGCACAAGCGCATCGCCGACATGCTCGCCAAGGGCGAGCCGTTGCCCGTCGACTTCAAGA
ACCGCGTGATCTACTACGTCGGGCCGGTCGATCCGGTGCGCGACGAAGTGGTCGGTCCGGCGGGCCCGACGACGGCCACG
CGCATGGACAAGTTCACCGAGACGATGCTCGCGCAGACGGGCCTCATCTCGATGGTCGGCAAGGCCGAGCGCGGCCCGGT
CGCGATCGACGCGATCAAGAAGCACAAGGCCGCTTATCTGATGGCGGTCGGCGGCGCCGCGTACCTCGTGTCGAAGGCGA
TTCGCGGCGCGAAGGTGCTCGCGTTCGAAGACCTCGGCATGGAAGCGATCTACGAGTTCGACGTACAGGACATGCCGGTG
ACGGTCGCGGTCGATTCGCAGGGCACGTCGGTCCATCAAACGGGGCCGAAGGAGTGGCAGGCGAAGATCGGCAAGATCCC
TGTCGCGAGCGCGTAA

Upstream 100 bases:

>100_bases
TTTCCACGAAGCGTCGACGCCACCGTCGGCGCCGCGCGAGCCGGCGCAACAGTCGGCCTGAGTCGATTCCCATACCCACC
GTCCTCACCGAGCCTCAGCC

Downstream 100 bases:

>100_bases
ATAAGAATGATTTTCAGGCCGGATTTCGATCCGGCCTTTTTGATGCGGCGGATCCGTTCCATCTTTCGGATATCGAACGG
ATTCGCCCTTGGCTTTTCAG

Product: tartrate/fumarate family Fe-S type hydro-lyase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MTVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEQSPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTIFVKVGM
DVRWASENGAATMSVTDMINEGVRRGYTHPDNVLRASIVNPPEGARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSEN
KSKFVMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMEPIDIQEIIARGPKDWVEELRVELHE
KVNALGIGAQGLGGLATVLDVKILAAPTHAASKPVAMIPNCAATRHAHFVLDGSGAAKLEAPSLDAWPKVQWEPNTETSK
RVDLNTLTPEEVASWKPGQTLLLSGKMLTGRDAAHKRIADMLAKGEPLPVDFKNRVIYYVGPVDPVRDEVVGPAGPTTAT
RMDKFTETMLAQTGLISMVGKAERGPVAIDAIKKHKAAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVQDMPV
TVAVDSQGTSVHQTGPKEWQAKIGKIPVASA

Sequences:

>Translated_511_residues
MTVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEQSPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTIFVKVGM
DVRWASENGAATMSVTDMINEGVRRGYTHPDNVLRASIVNPPEGARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSEN
KSKFVMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMEPIDIQEIIARGPKDWVEELRVELHE
KVNALGIGAQGLGGLATVLDVKILAAPTHAASKPVAMIPNCAATRHAHFVLDGSGAAKLEAPSLDAWPKVQWEPNTETSK
RVDLNTLTPEEVASWKPGQTLLLSGKMLTGRDAAHKRIADMLAKGEPLPVDFKNRVIYYVGPVDPVRDEVVGPAGPTTAT
RMDKFTETMLAQTGLISMVGKAERGPVAIDAIKKHKAAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVQDMPV
TVAVDSQGTSVHQTGPKEWQAKIGKIPVASA
>Mature_510_residues
TVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEQSPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTIFVKVGMD
VRWASENGAATMSVTDMINEGVRRGYTHPDNVLRASIVNPPEGARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENK
SKFVMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMEPIDIQEIIARGPKDWVEELRVELHEK
VNALGIGAQGLGGLATVLDVKILAAPTHAASKPVAMIPNCAATRHAHFVLDGSGAAKLEAPSLDAWPKVQWEPNTETSKR
VDLNTLTPEEVASWKPGQTLLLSGKMLTGRDAAHKRIADMLAKGEPLPVDFKNRVIYYVGPVDPVRDEVVGPAGPTTATR
MDKFTETMLAQTGLISMVGKAERGPVAIDAIKKHKAAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVQDMPVT
VAVDSQGTSVHQTGPKEWQAKIGKIPVASA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=472, Percent_Identity=33.0508474576271, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1790564, Length=471, Percent_Identity=31.8471337579618, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1789442, Length=287, Percent_Identity=27.5261324041812, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=27.9329608938547, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54834; Mature: 54703

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEQSPAAKDAIAQILTNSRMCAE
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHC
GKRPICQDTGIVTIFVKVGMDVRWASENGAATMSVTDMINEGVRRGYTHPDNVLRASIVN
CCCCCCCCCCEEEEEEEECCEEEECCCCCCEEEHHHHHHHHHHHCCCCCCCHHHEEECCC
PPEGARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFVMLNPSDSIVDWVLKT
CCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHH
VPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMEPIDIQEIIARGPKDWVEELRVELHE
HCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHH
KVNALGIGAQGLGGLATVLDVKILAAPTHAASKPVAMIPNCAATRHAHFVLDGSGAAKLE
HHHHEECCCCCCCHHHHHHEEEEEECCCCCCCCCEEECCCCCCCCCEEEEEECCCCCEEE
APSLDAWPKVQWEPNTETSKRVDLNTLTPEEVASWKPGQTLLLSGKMLTGRDAAHKRIAD
CCCCCCCCCEEECCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECCEECCCHHHHHHHHH
MLAKGEPLPVDFKNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTETMLAQTGLISMVG
HHHCCCCCCEECCCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
KAERGPVAIDAIKKHKAAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVQDMPV
CCCCCCEEHHHHHHCCEEEEEEECHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCE
TVAVDSQGTSVHQTGPKEWQAKIGKIPVASA
EEEECCCCCEECCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEQSPAAKDAIAQILTNSRMCAE
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHC
GKRPICQDTGIVTIFVKVGMDVRWASENGAATMSVTDMINEGVRRGYTHPDNVLRASIVN
CCCCCCCCCCEEEEEEEECCEEEECCCCCCEEEHHHHHHHHHHHCCCCCCCHHHEEECCC
PPEGARKNTKDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFVMLNPSDSIVDWVLKT
CCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHH
VPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMEPIDIQEIIARGPKDWVEELRVELHE
HCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHH
KVNALGIGAQGLGGLATVLDVKILAAPTHAASKPVAMIPNCAATRHAHFVLDGSGAAKLE
HHHHEECCCCCCCHHHHHHEEEEEECCCCCCCCCEEECCCCCCCCCEEEEEECCCCCEEE
APSLDAWPKVQWEPNTETSKRVDLNTLTPEEVASWKPGQTLLLSGKMLTGRDAAHKRIAD
CCCCCCCCCEEECCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECCEECCCHHHHHHHHH
MLAKGEPLPVDFKNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTETMLAQTGLISMVG
HHHCCCCCCEECCCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
KAERGPVAIDAIKKHKAAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAIYEFDVQDMPV
CCCCCCEEHHHHHHCCEEEEEEECHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCE
TVAVDSQGTSVHQTGPKEWQAKIGKIPVASA
EEEECCCCCEECCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]