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The map label for this gene is cobA-2 [H]

Identifier: 126447693

GI number: 126447693

Start: 1219515

End: 1220330

Strand: Direct

Name: cobA-2 [H]

Synonym: BMA10247_A1254

Alternate gene names: 126447693

Gene position: 1219515-1220330 (Clockwise)

Preceding gene: 126447497

Following gene: 126447845

Centisome position: 51.83

GC content: 74.39

Gene sequence:

>816_bases
ATGGGCAAAGTCACACTGCTCGGCGCCGGCCCCGGCGATCTGGATCTGCTGACGATGAAGGCGGCGAAGGCGCTCGCGGC
CGCGGACGTGCTGCTGCTCGACGATCTCGTCGATCCGGGCATCGTCGCGCTCGCGCCGCGCGCGCGCGTGATCCGCGTCG
GCAAGCGCGGCGGCTGCCGCTCGACGCCTCAGGCGTTCATCGAGCGGCTGATGTGCCGCTACGCGCTGCGCGGCGCGCAC
GTCGTGCGCGTGAAGGGCGGCGACGTGCTGCTGTTCGGCCGCGCGGGCGAGGAACTCGCCGCGTTGCGCGCGGCGCGCGT
GCCGGTCGAGATCGTCAACGGCATCTCGTCGGGCTTCGCGGCCGCGGCGAGCCTCGGCGTGTCGCTCACGCACCGCGATC
ACTGCCAGGGCGTGACGTTCGTCACCGCGCACCTGCAGGACCATGGCGAGCCCGACTGGGCAAGCCTCGCCGCGACCGGC
ACGACGCTCGCGATCTACATGGGAATGAGCCGCATCGAGCGGATCGCGGCGGGGCTGCTGTCGACGCTCGACGACAAGAC
GCCCGCGGCCGTCGTCCAGTGGGCGGGCGCGCCGGCCGAGCGGCGCTGGACCGGCACGCTCGGGCACCTTGCATCCGGCG
CCGCCGAAGCGGGGCTCGGCAGCCCGGCCGTGATCTTCGTCGGCCGCGCGATCGGCGAGGCGCTCGCGATCGGCGACGCG
CACGCGGCGCCGCATGCGAATGCGCACGCGCTTGCGGACGACGCGCACGCCGCCTTGCACGCGGCCGAGCGCCGCCGAAT
CTCGCACGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCTCGTCGGCCGGGTGCGCCGGACGCCGTTCTTTTTTGGCGGGGCGCATTCGCCGCGAATGCGCCGCACGCACGGCATG
GGAACACGAAGATGAACACG

Downstream 100 bases:

>100_bases
TCGGCGCGAGCCGGCGCGGCGCACCGCGCCCGCGCGCGTATGGTAGAAACAAAGGCATGGGCGAGCGCCGCCGCGACGCG
CCCGCCTCTTCCGTCTGACA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MGKVTLLGAGPGDLDLLTMKAAKALAAADVLLLDDLVDPGIVALAPRARVIRVGKRGGCRSTPQAFIERLMCRYALRGAH
VVRVKGGDVLLFGRAGEELAALRAARVPVEIVNGISSGFAAAASLGVSLTHRDHCQGVTFVTAHLQDHGEPDWASLAATG
TTLAIYMGMSRIERIAAGLLSTLDDKTPAAVVQWAGAPAERRWTGTLGHLASGAAEAGLGSPAVIFVGRAIGEALAIGDA
HAAPHANAHALADDAHAALHAAERRRISHAA

Sequences:

>Translated_271_residues
MGKVTLLGAGPGDLDLLTMKAAKALAAADVLLLDDLVDPGIVALAPRARVIRVGKRGGCRSTPQAFIERLMCRYALRGAH
VVRVKGGDVLLFGRAGEELAALRAARVPVEIVNGISSGFAAAASLGVSLTHRDHCQGVTFVTAHLQDHGEPDWASLAATG
TTLAIYMGMSRIERIAAGLLSTLDDKTPAAVVQWAGAPAERRWTGTLGHLASGAAEAGLGSPAVIFVGRAIGEALAIGDA
HAAPHANAHALADDAHAALHAAERRRISHAA
>Mature_270_residues
GKVTLLGAGPGDLDLLTMKAAKALAAADVLLLDDLVDPGIVALAPRARVIRVGKRGGCRSTPQAFIERLMCRYALRGAHV
VRVKGGDVLLFGRAGEELAALRAARVPVEIVNGISSGFAAAASLGVSLTHRDHCQGVTFVTAHLQDHGEPDWASLAATGT
TLAIYMGMSRIERIAAGLLSTLDDKTPAAVVQWAGAPAERRWTGTLGHLASGAAEAGLGSPAVIFVGRAIGEALAIGDAH
AAPHANAHALADDAHAALHAAERRRISHAA

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=233, Percent_Identity=41.2017167381974, Blast_Score=169, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322922, Length=236, Percent_Identity=29.6610169491525, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 27841; Mature: 27710

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVTLLGAGPGDLDLLTMKAAKALAAADVLLLDDLVDPGIVALAPRARVIRVGKRGGCR
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCC
STPQAFIERLMCRYALRGAHVVRVKGGDVLLFGRAGEELAALRAARVPVEIVNGISSGFA
CCHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHH
AAASLGVSLTHRDHCQGVTFVTAHLQDHGEPDWASLAATGTTLAIYMGMSRIERIAAGLL
HHHHHCEEEECHHHCCCEEEEEEEHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
STLDDKTPAAVVQWAGAPAERRWTGTLGHLASGAAEAGLGSPAVIFVGRAIGEALAIGDA
HHHCCCCCHHHEEECCCCCCCCCCCHHHHHHCCHHHCCCCCCCEEEEHHHHHHHHHCCCC
HAAPHANAHALADDAHAALHAAERRRISHAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKVTLLGAGPGDLDLLTMKAAKALAAADVLLLDDLVDPGIVALAPRARVIRVGKRGGCR
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCC
STPQAFIERLMCRYALRGAHVVRVKGGDVLLFGRAGEELAALRAARVPVEIVNGISSGFA
CCHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHH
AAASLGVSLTHRDHCQGVTFVTAHLQDHGEPDWASLAATGTTLAIYMGMSRIERIAAGLL
HHHHHCEEEECHHHCCCEEEEEEEHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
STLDDKTPAAVVQWAGAPAERRWTGTLGHLASGAAEAGLGSPAVIFVGRAIGEALAIGDA
HHHCCCCCHHHEEECCCCCCCCCCCHHHHHHCCHHHCCCCCCCEEEEHHHHHHHHHCCCC
HAAPHANAHALADDAHAALHAAERRRISHAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]