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The map label for this gene is yfjR [H]

Identifier: 126447586

GI number: 126447586

Start: 2219138

End: 2220037

Strand: Reverse

Name: yfjR [H]

Synonym: BMA10247_A2287

Alternate gene names: 126447586

Gene position: 2220037-2219138 (Counterclockwise)

Preceding gene: 126447703

Following gene: 126445717

Centisome position: 94.36

GC content: 70.44

Gene sequence:

>900_bases
ATGGAGGCGATCATGAAACTAGGCTTCGTCGGCCTCGGTGTGATGGGGCAGCCGATGGCGCTCAATCTCGCGCGGGCCGG
AACCGAGCTCGTGGTGTGGAACCGCACGCGCGAGCGCTGCGAGCCGTTGCGCGCGGCCGGCGCGCAAGTCGCCGATTCGG
CGGCCGACGTCTATCGGCGCGCGCGCATCGTCATCCTGATGATGGCGACCGATGCCGCGATCGACGCGGTGCTCGACCGC
GGCAAGCCCGCGTTCGCGAGCAACGTCGCGCAACACACGATCGTCCAGATGGGGACGGTGTCGGCCGAGTATTCGCGCGG
CCTCGAGGCCGACATTCGTGCGGCGGGCGGCCGCTATGTCGAGGCGCCGGTGTCCGGCTCGCGCCAGCCGGCCGAGGCCG
GGCGGCTCGTCGCGATGCTGGCGGGGGAGCCGGCCGCCGTCGAGGAGGTGCGCGCTCTGCTGGCGCCGATGTGCCGCGAG
ATCGTCGCGACGGGCCAGGTGCCGAGCGGCCTGCTGATGAAGCTTGCCGTCAACACGTTCCTGATCGCGATGGTGACGGG
GCTCGCGGAAGCCGCGCACGCGGCGCGCGGCTTCGGGCTCGACATGAAGCAGTTCCAGGCGGTGCTCGATGCGGGGCCGA
TGGCGAGCAGCGTGTCGCGCGTGAAGATCGACAAGCTCGTCAACGAGGATTTCGAGGTTCAGGCGTCGATCGTCGATGTG
TTCAAGAACAGCCGGCTCGCGACCGAGGCCGCGCACGGCGCGCATCTCGCGGCGCCGCTGCTCGAAGTCTGCTGCGAGCT
GTATCGCGAGACCGAGGCGCTCGGGCACGGGCAAGCGGACATGGCGGCCGTCGTGCATGCGATCGAGGCGCGCAGCGTCG
CGCGCTACGCGGGCAACTGA

Upstream 100 bases:

>100_bases
GGCTTGCACGCCGTCGCCAGCCCGGGCGCCCGGCCGACTGAAGCGCTCCGATTGCCCGCAACCTGTCGATGCGCCGCCGC
CCGCGTGCGGCGGCGAACTC

Downstream 100 bases:

>100_bases
CGCGCGCGGCGAATCTGCGCAAATCGCGACGAAACAGGGCCTGCGGCTTCGTCGCGCGCGCCGCGCGCCGGATCGCGCGC
CCCGCCCCCCGCGCTTTTTC

Product: NAD-binding protein

Products: (R)-Glycerate; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MEAIMKLGFVGLGVMGQPMALNLARAGTELVVWNRTRERCEPLRAAGAQVADSAADVYRRARIVILMMATDAAIDAVLDR
GKPAFASNVAQHTIVQMGTVSAEYSRGLEADIRAAGGRYVEAPVSGSRQPAEAGRLVAMLAGEPAAVEEVRALLAPMCRE
IVATGQVPSGLLMKLAVNTFLIAMVTGLAEAAHAARGFGLDMKQFQAVLDAGPMASSVSRVKIDKLVNEDFEVQASIVDV
FKNSRLATEAAHGAHLAAPLLEVCCELYRETEALGHGQADMAAVVHAIEARSVARYAGN

Sequences:

>Translated_299_residues
MEAIMKLGFVGLGVMGQPMALNLARAGTELVVWNRTRERCEPLRAAGAQVADSAADVYRRARIVILMMATDAAIDAVLDR
GKPAFASNVAQHTIVQMGTVSAEYSRGLEADIRAAGGRYVEAPVSGSRQPAEAGRLVAMLAGEPAAVEEVRALLAPMCRE
IVATGQVPSGLLMKLAVNTFLIAMVTGLAEAAHAARGFGLDMKQFQAVLDAGPMASSVSRVKIDKLVNEDFEVQASIVDV
FKNSRLATEAAHGAHLAAPLLEVCCELYRETEALGHGQADMAAVVHAIEARSVARYAGN
>Mature_299_residues
MEAIMKLGFVGLGVMGQPMALNLARAGTELVVWNRTRERCEPLRAAGAQVADSAADVYRRARIVILMMATDAAIDAVLDR
GKPAFASNVAQHTIVQMGTVSAEYSRGLEADIRAAGGRYVEAPVSGSRQPAEAGRLVAMLAGEPAAVEEVRALLAPMCRE
IVATGQVPSGLLMKLAVNTFLIAMVTGLAEAAHAARGFGLDMKQFQAVLDAGPMASSVSRVKIDKLVNEDFEVQASIVDV
FKNSRLATEAAHGAHLAAPLLEVCCELYRETEALGHGQADMAAVVHAIEARSVARYAGN

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=288, Percent_Identity=29.5138888888889, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI23308751, Length=289, Percent_Identity=27.3356401384083, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=30.4195804195804, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI145693186, Length=287, Percent_Identity=27.1777003484321, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790315, Length=286, Percent_Identity=26.9230769230769, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17557316, Length=206, Percent_Identity=28.1553398058252, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574115, Length=279, Percent_Identity=27.5985663082437, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24655230, Length=287, Percent_Identity=30.3135888501742, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922568, Length=287, Percent_Identity=30.3135888501742, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.60 [C]

Molecular weight: Translated: 31537; Mature: 31537

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAIMKLGFVGLGVMGQPMALNLARAGTELVVWNRTRERCEPLRAAGAQVADSAADVYRR
CHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHCHHHHHHHHHHHHH
ARIVILMMATDAAIDAVLDRGKPAFASNVAQHTIVQMGTVSAEYSRGLEADIRAAGGRYV
HHEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHCCCCEE
EAPVSGSRQPAEAGRLVAMLAGEPAAVEEVRALLAPMCREIVATGQVPSGLLMKLAVNTF
ECCCCCCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LIAMVTGLAEAAHAARGFGLDMKQFQAVLDAGPMASSVSRVKIDKLVNEDFEVQASIVDV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
FKNSRLATEAAHGAHLAAPLLEVCCELYRETEALGHGQADMAAVVHAIEARSVARYAGN
HHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEAIMKLGFVGLGVMGQPMALNLARAGTELVVWNRTRERCEPLRAAGAQVADSAADVYRR
CHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHCHHHHHHHHHHHHH
ARIVILMMATDAAIDAVLDRGKPAFASNVAQHTIVQMGTVSAEYSRGLEADIRAAGGRYV
HHEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHCCCCEE
EAPVSGSRQPAEAGRLVAMLAGEPAAVEEVRALLAPMCREIVATGQVPSGLLMKLAVNTF
ECCCCCCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LIAMVTGLAEAAHAARGFGLDMKQFQAVLDAGPMASSVSRVKIDKLVNEDFEVQASIVDV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
FKNSRLATEAAHGAHLAAPLLEVCCELYRETEALGHGQADMAAVVHAIEARSVARYAGN
HHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Hydroxy-3-oxopropanoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: 2-Hydroxy-3-oxopropanoate + H+ + Nicotinamide adenine dinucleotide - reduced --> (R)-Glycerate + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]