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The map label for this gene is pycA [H]

Identifier: 126447049

GI number: 126447049

Start: 2090444

End: 2091889

Strand: Direct

Name: pycA [H]

Synonym: BMA10247_A2158

Alternate gene names: 126447049

Gene position: 2090444-2091889 (Clockwise)

Preceding gene: 126446269

Following gene: 126447227

Centisome position: 88.85

GC content: 71.65

Gene sequence:

>1446_bases
ATGAACGACCTCGATTCGACCCACGCTTCGTTTTACCGGCCGTCCCGGATCGGTACGGTGCTCGTCGCCAATCGCGGCGA
GATCGCGGTGCGCGTGATTCGCGCCGCGCACGAGCTCGGGATGCGCGCGGTCGCCGCGGTCAGCGACGCGGACCGCGACA
GCCTCGCCGCGCGGATGGCCGACGAAGCGGTGCACATCGGCCCGTCGCACGCGGCGAAGAGCTATCTGGATCCGGCCGCG
ATGCTCGCCGCCGCGCAGCAATGCGGCGCCGACGCGATTCATCCGGGCTACGGCTTTCTGTCGGAGAACGCGGACTTCGC
CGCGCAGGTCGAAGCCGCCGGCCTGATCTTCGTCGGTCCGCCCGCGCGCGTGATCGCGACGATGGGCGACAAGGCCCGCG
CGCGCGAAACCGCGCGGCGCGCCGGCGTGCCGACGGTGCCGGGCAGCGAAGGCGTCGTCGCGTCGCTCGACGGCGCGCAC
GAGGTGGCCGCGCGCATCGGCTACCCGGTGATGATCAAGGCGGCCGCGGGCGGCGGCGGGCGCGGTATCCGCGTCGCGCA
CGACGCCGCGCAGCTCGCCGCGCAGCTACCGCTCGCGCAGCGCGAGGCACAGGCCGCGTTCGGCGACGGCGGCGTCTATC
TCGAACGCTTCATCGCGCGCGCGCGGCACATCGAAGTGCAGATCCTGGGCGACGGCGAGAACGTTATTCATCTGTTCGAA
CGCGAATGCTCGCTGCAGCGGCGGCGCCAGAAGATTCTCGAGGAAGCGCCGTCTCCGTCGCTCACGCCCGCGCTGCGCGA
TGCGCTGTGCGCATCGGCGACGCGTCTCGCGCGGCAGGTGGGCTATCGCGGCGCGGGCACGCTCGAGTATCTGTTCGACG
ACGCGCGCGGCGAGTTCTACTTCATCGAGATGAACACGCGCATCCAGGTCGAGCATCCGGTGACGGAGGCGGTCACGGGC
GTCGATCTCGTGCGCGAGATGTTGCGCATCGCGGACGGCGAGCCGCTGCGCTTCGCGCAGGGCGACATCGCACTGCGCGG
CGCCGCGCTCGAATGCCGGATCAACGCGGAGGACCCGCTGCAGGATTTCCGGCCCAACCCCGGCCGCATCGATGCGCTCG
TCTGGCCCGCGGGCCCCGGCGTTCGAATCGATTCGCTGCTCTATCCGGGCTACACGGTGCCGCCGTTCTACGATTCGCTG
CTCGCGAAGCTGATCGTGCACGACGAAAGCCGGCCGGCCGCGCTCGCGCGCGCGGCGCGCGCGCTGCGCGAGCTGCGCAT
CGACGGCGTGAAGACCACCGCGCCGCTGCATCGCGCGCTGCTCGACGACGCCGACGTGCGCGCGGGCCGCTATCACACGA
ACTATCTGGAGGCCTGGATGCGCGACTGGCTCGCGCGGCTCGACGCCCGCGCGAGCGCGCCGCGTGGCCTGGGAAAGGCA
GCATGA

Upstream 100 bases:

>100_bases
TCGAGACGGCGGCCGCGGGCCGCGTCGTCGAGATTCTGGTCGACGACGGCGAGCCCGTCGACGCGGGCCAGGTGCTGATG
CGCACGGAGGAGTGAGCGCG

Downstream 100 bases:

>100_bases
CGATCCGATACACATTCGGCGGCGACGAGTTCATCTTCGTCGAGATCAGCGAATCGATGTCGCTCGACGCGTTCTTCAAG
GGCACCGCGATCACGCGCGA

Product: acetyl-CoA carboxylase biotin carboxylase subunit

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MNDLDSTHASFYRPSRIGTVLVANRGEIAVRVIRAAHELGMRAVAAVSDADRDSLAARMADEAVHIGPSHAAKSYLDPAA
MLAAAQQCGADAIHPGYGFLSENADFAAQVEAAGLIFVGPPARVIATMGDKARARETARRAGVPTVPGSEGVVASLDGAH
EVAARIGYPVMIKAAAGGGGRGIRVAHDAAQLAAQLPLAQREAQAAFGDGGVYLERFIARARHIEVQILGDGENVIHLFE
RECSLQRRRQKILEEAPSPSLTPALRDALCASATRLARQVGYRGAGTLEYLFDDARGEFYFIEMNTRIQVEHPVTEAVTG
VDLVREMLRIADGEPLRFAQGDIALRGAALECRINAEDPLQDFRPNPGRIDALVWPAGPGVRIDSLLYPGYTVPPFYDSL
LAKLIVHDESRPAALARAARALRELRIDGVKTTAPLHRALLDDADVRAGRYHTNYLEAWMRDWLARLDARASAPRGLGKA
A

Sequences:

>Translated_481_residues
MNDLDSTHASFYRPSRIGTVLVANRGEIAVRVIRAAHELGMRAVAAVSDADRDSLAARMADEAVHIGPSHAAKSYLDPAA
MLAAAQQCGADAIHPGYGFLSENADFAAQVEAAGLIFVGPPARVIATMGDKARARETARRAGVPTVPGSEGVVASLDGAH
EVAARIGYPVMIKAAAGGGGRGIRVAHDAAQLAAQLPLAQREAQAAFGDGGVYLERFIARARHIEVQILGDGENVIHLFE
RECSLQRRRQKILEEAPSPSLTPALRDALCASATRLARQVGYRGAGTLEYLFDDARGEFYFIEMNTRIQVEHPVTEAVTG
VDLVREMLRIADGEPLRFAQGDIALRGAALECRINAEDPLQDFRPNPGRIDALVWPAGPGVRIDSLLYPGYTVPPFYDSL
LAKLIVHDESRPAALARAARALRELRIDGVKTTAPLHRALLDDADVRAGRYHTNYLEAWMRDWLARLDARASAPRGLGKA
A
>Mature_481_residues
MNDLDSTHASFYRPSRIGTVLVANRGEIAVRVIRAAHELGMRAVAAVSDADRDSLAARMADEAVHIGPSHAAKSYLDPAA
MLAAAQQCGADAIHPGYGFLSENADFAAQVEAAGLIFVGPPARVIATMGDKARARETARRAGVPTVPGSEGVVASLDGAH
EVAARIGYPVMIKAAAGGGGRGIRVAHDAAQLAAQLPLAQREAQAAFGDGGVYLERFIARARHIEVQILGDGENVIHLFE
RECSLQRRRQKILEEAPSPSLTPALRDALCASATRLARQVGYRGAGTLEYLFDDARGEFYFIEMNTRIQVEHPVTEAVTG
VDLVREMLRIADGEPLRFAQGDIALRGAALECRINAEDPLQDFRPNPGRIDALVWPAGPGVRIDSLLYPGYTVPPFYDSL
LAKLIVHDESRPAALARAARALRELRIDGVKTTAPLHRALLDDADVRAGRYHTNYLEAWMRDWLARLDARASAPRGLGKA
A

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI189095269, Length=440, Percent_Identity=47.5, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI65506442, Length=440, Percent_Identity=47.5, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI295821183, Length=440, Percent_Identity=47.5, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI116805327, Length=468, Percent_Identity=44.6581196581197, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI106049528, Length=452, Percent_Identity=45.7964601769911, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI106049295, Length=452, Percent_Identity=45.7964601769911, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI106049292, Length=452, Percent_Identity=45.7964601769911, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI38679971, Length=503, Percent_Identity=32.4055666003976, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI38679960, Length=503, Percent_Identity=32.4055666003976, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI38679974, Length=503, Percent_Identity=32.4055666003976, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI38679977, Length=503, Percent_Identity=32.4055666003976, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI38679967, Length=503, Percent_Identity=32.4055666003976, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI134142062, Length=521, Percent_Identity=31.4779270633397, Blast_Score=213, Evalue=4e-55,
Organism=Escherichia coli, GI1789654, Length=443, Percent_Identity=52.5959367945824, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1786216, Length=244, Percent_Identity=24.5901639344262, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17567343, Length=448, Percent_Identity=46.4285714285714, Blast_Score=383, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71987519, Length=468, Percent_Identity=45.5128205128205, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17562816, Length=448, Percent_Identity=46.4285714285714, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71997168, Length=509, Percent_Identity=29.6660117878193, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI71997163, Length=509, Percent_Identity=29.4695481335953, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI133931226, Length=486, Percent_Identity=29.2181069958848, Blast_Score=196, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6319685, Length=442, Percent_Identity=43.4389140271493, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6319695, Length=446, Percent_Identity=45.067264573991, Blast_Score=356, Evalue=5e-99,
Organism=Saccharomyces cerevisiae, GI6321376, Length=446, Percent_Identity=45.067264573991, Blast_Score=350, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6324343, Length=510, Percent_Identity=30.5882352941176, Blast_Score=212, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6323863, Length=465, Percent_Identity=31.3978494623656, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24652212, Length=452, Percent_Identity=46.4601769911504, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652210, Length=452, Percent_Identity=46.4601769911504, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652214, Length=452, Percent_Identity=46.4601769911504, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI19921944, Length=452, Percent_Identity=46.4601769911504, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652216, Length=452, Percent_Identity=46.4601769911504, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281363050, Length=452, Percent_Identity=46.4601769911504, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652224, Length=452, Percent_Identity=46.4601769911504, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652222, Length=452, Percent_Identity=46.4601769911504, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652220, Length=452, Percent_Identity=46.4601769911504, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652218, Length=452, Percent_Identity=46.4601769911504, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24651757, Length=443, Percent_Identity=42.8893905191874, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI24651759, Length=405, Percent_Identity=41.4814814814815, Blast_Score=311, Evalue=4e-85,
Organism=Drosophila melanogaster, GI161076409, Length=502, Percent_Identity=31.2749003984064, Blast_Score=211, Evalue=8e-55,
Organism=Drosophila melanogaster, GI24586458, Length=502, Percent_Identity=31.2749003984064, Blast_Score=211, Evalue=9e-55,
Organism=Drosophila melanogaster, GI161076407, Length=502, Percent_Identity=31.2749003984064, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24586460, Length=502, Percent_Identity=31.2749003984064, Blast_Score=211, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 51740; Mature: 51740

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLDSTHASFYRPSRIGTVLVANRGEIAVRVIRAAHELGMRAVAAVSDADRDSLAARMA
CCCCCCCHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DEAVHIGPSHAAKSYLDPAAMLAAAQQCGADAIHPGYGFLSENADFAAQVEAAGLIFVGP
HHHEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECC
PARVIATMGDKARARETARRAGVPTVPGSEGVVASLDGAHEVAARIGYPVMIKAAAGGGG
CHHHEEECCCHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHCCCCEEEEEECCCCC
RGIRVAHDAAQLAAQLPLAQREAQAAFGDGGVYLERFIARARHIEVQILGDGENVIHLFE
CCEEEEHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEEECCCHHHHHHH
RECSLQRRRQKILEEAPSPSLTPALRDALCASATRLARQVGYRGAGTLEYLFDDARGEFY
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEE
FIEMNTRIQVEHPVTEAVTGVDLVREMLRIADGEPLRFAQGDIALRGAALECRINAEDPL
EEEECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEECEEEEEEECCCCCH
QDFRPNPGRIDALVWPAGPGVRIDSLLYPGYTVPPFYDSLLAKLIVHDESRPAALARAAR
HHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
ALRELRIDGVKTTAPLHRALLDDADVRAGRYHTNYLEAWMRDWLARLDARASAPRGLGKA
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
A
C
>Mature Secondary Structure
MNDLDSTHASFYRPSRIGTVLVANRGEIAVRVIRAAHELGMRAVAAVSDADRDSLAARMA
CCCCCCCHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DEAVHIGPSHAAKSYLDPAAMLAAAQQCGADAIHPGYGFLSENADFAAQVEAAGLIFVGP
HHHEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECC
PARVIATMGDKARARETARRAGVPTVPGSEGVVASLDGAHEVAARIGYPVMIKAAAGGGG
CHHHEEECCCHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHCCCCEEEEEECCCCC
RGIRVAHDAAQLAAQLPLAQREAQAAFGDGGVYLERFIARARHIEVQILGDGENVIHLFE
CCEEEEHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEEECCCHHHHHHH
RECSLQRRRQKILEEAPSPSLTPALRDALCASATRLARQVGYRGAGTLEYLFDDARGEFY
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEE
FIEMNTRIQVEHPVTEAVTGVDLVREMLRIADGEPLRFAQGDIALRGAALECRINAEDPL
EEEECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEECEEEEEEECCCCCH
QDFRPNPGRIDALVWPAGPGVRIDSLLYPGYTVPPFYDSLLAKLIVHDESRPAALARAAR
HHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
ALRELRIDGVKTTAPLHRALLDDADVRAGRYHTNYLEAWMRDWLARLDARASAPRGLGKA
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 9478969 [H]