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The map label for this gene is rafR [H]

Identifier: 126446305

GI number: 126446305

Start: 1910457

End: 1911473

Strand: Reverse

Name: rafR [H]

Synonym: BMA10247_A1982

Alternate gene names: 126446305

Gene position: 1911473-1910457 (Counterclockwise)

Preceding gene: 126446576

Following gene: 126447029

Centisome position: 81.25

GC content: 70.6

Gene sequence:

>1017_bases
GTGAAAGTGAATCTGAAGGCGTTGTCGGAGGCGCTCGGTCTGTCGCGCACGACGGTGAGCCGCGCGCTGAACGGCTACGA
CGACGTGAGCGAAGCCACGCGCGAGCGCGTGATGCAGGCGGCGCGCGAGTTCGGCTACGTCGCCGATCCGACCGCGCGCC
GGCTCGCGACCGGGCGCGCGGACGCGGTCGGCATCGTCTATCCGTTCGGCGCGGGCGACCTCGGTGATCCGCGCTTCGCC
GAGGTCGTCGCGGGCGTCACGGAGCGTCTCGGCGAGAGCGGCCTCGATTTCTTCATCGTGTCCGCGCGCCCGAACGCGGA
GCTCGACACGTACCGGCGGCTCGTCGACGGCCGGCTCGTCGACGGGCTGATCGTCGCGCGCACGCTCGTCGACGATCCGC
GCCTCCGTTTTCTTCAGGAACGCGAATTTCCGTTCGTCGCCTATGGCCGGACCGCGAGCGCGAAGCCGTACACCTGGTTC
GATTTCGATAACGAGGCGGGCATGCACGCGGCCGCGCAACGGCTGACCGCGTTCGGGCATCGGCGCATCGCGCTCGTTTG
CGCGCCGCGGACGCTGAATTTCGCCGCGCAGCGGCGCGCGGGCTTCGAGCGCGCGCTGCGAGAGGCTGGGATCGAGCCCG
ATGCCGCGCTGATCGTCGAATGCGCGTTCACGCGTGACGGCGGCTACGGCGCGGCGCAAACGCTGCTCGCCCTCGAGCGT
GCGCCGAGCGCGATCGTCGTCGACAACAACATCGCCGGCGCGGGCGTGTTCAGGGCGATCGTCGAGCGCGGCAGGCGCTT
CGTTCGCGACGTATCGCTGATCGTCTATGACGGCGTGTCGCCCGACGTTTCGTTTCCGCACCACGCGAGCGCGGTGCTGC
AGCCGACCGGGCATGCATCCGGCCGCGCGATCGCCGAACTGATGCTCGGCGCCATCGCGCATCCGGGCGACGCCGCGCAT
CGGCTCGTGTCGCCCGTCATCGAGCCGGGCGACACGGACGGGCCGTTCAGCGGCTGA

Upstream 100 bases:

>100_bases
GGCGTCAAAAGTAATTTATTTGACGGAATGCCCGACAATTGGGAAACAATGGCCGGCGGCGGCGCCGGCCGAATGCAGGG
AGACCATTCGAGGTGAGAGC

Downstream 100 bases:

>100_bases
CGCGTACGAGATGCGGGACGCCGCCGCGCGATCGAGGCGTCGCGCAGTTCGATGTTGCGCGACGCGATGTGCGCGTTACG
CTGCGTTGACCGTTGACCGT

Product: putative raffinose repressor

Products: NA

Alternate protein names: Raffinose operon repressor [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKVNLKALSEALGLSRTTVSRALNGYDDVSEATRERVMQAAREFGYVADPTARRLATGRADAVGIVYPFGAGDLGDPRFA
EVVAGVTERLGESGLDFFIVSARPNAELDTYRRLVDGRLVDGLIVARTLVDDPRLRFLQEREFPFVAYGRTASAKPYTWF
DFDNEAGMHAAAQRLTAFGHRRIALVCAPRTLNFAAQRRAGFERALREAGIEPDAALIVECAFTRDGGYGAAQTLLALER
APSAIVVDNNIAGAGVFRAIVERGRRFVRDVSLIVYDGVSPDVSFPHHASAVLQPTGHASGRAIAELMLGAIAHPGDAAH
RLVSPVIEPGDTDGPFSG

Sequences:

>Translated_338_residues
MKVNLKALSEALGLSRTTVSRALNGYDDVSEATRERVMQAAREFGYVADPTARRLATGRADAVGIVYPFGAGDLGDPRFA
EVVAGVTERLGESGLDFFIVSARPNAELDTYRRLVDGRLVDGLIVARTLVDDPRLRFLQEREFPFVAYGRTASAKPYTWF
DFDNEAGMHAAAQRLTAFGHRRIALVCAPRTLNFAAQRRAGFERALREAGIEPDAALIVECAFTRDGGYGAAQTLLALER
APSAIVVDNNIAGAGVFRAIVERGRRFVRDVSLIVYDGVSPDVSFPHHASAVLQPTGHASGRAIAELMLGAIAHPGDAAH
RLVSPVIEPGDTDGPFSG
>Mature_338_residues
MKVNLKALSEALGLSRTTVSRALNGYDDVSEATRERVMQAAREFGYVADPTARRLATGRADAVGIVYPFGAGDLGDPRFA
EVVAGVTERLGESGLDFFIVSARPNAELDTYRRLVDGRLVDGLIVARTLVDDPRLRFLQEREFPFVAYGRTASAKPYTWF
DFDNEAGMHAAAQRLTAFGHRRIALVCAPRTLNFAAQRRAGFERALREAGIEPDAALIVECAFTRDGGYGAAQTLLALER
APSAIVVDNNIAGAGVFRAIVERGRRFVRDVSLIVYDGVSPDVSFPHHASAVLQPTGHASGRAIAELMLGAIAHPGDAAH
RLVSPVIEPGDTDGPFSG

Specific function: The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor to raf operator (rafO) DNA. Binds raffinose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=29.585798816568, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790369, Length=282, Percent_Identity=28.3687943262411, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787580, Length=269, Percent_Identity=24.907063197026, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1790194, Length=339, Percent_Identity=24.7787610619469, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787906, Length=336, Percent_Identity=25.8928571428571, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1786540, Length=284, Percent_Identity=27.8169014084507, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1789202, Length=277, Percent_Identity=27.4368231046931, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788474, Length=280, Percent_Identity=26.7857142857143, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789068, Length=284, Percent_Identity=26.056338028169, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI48994940, Length=225, Percent_Identity=24, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36349; Mature: 36349

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNLKALSEALGLSRTTVSRALNGYDDVSEATRERVMQAAREFGYVADPTARRLATGRA
CCCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCC
DAVGIVYPFGAGDLGDPRFAEVVAGVTERLGESGLDFFIVSARPNAELDTYRRLVDGRLV
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHH
DGLIVARTLVDDPRLRFLQEREFPFVAYGRTASAKPYTWFDFDNEAGMHAAAQRLTAFGH
HHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHCC
RRIALVCAPRTLNFAAQRRAGFERALREAGIEPDAALIVECAFTRDGGYGAAQTLLALER
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHH
APSAIVVDNNIAGAGVFRAIVERGRRFVRDVSLIVYDGVSPDVSFPHHASAVLQPTGHAS
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCEECCCCCCC
GRAIAELMLGAIAHPGDAAHRLVSPVIEPGDTDGPFSG
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKVNLKALSEALGLSRTTVSRALNGYDDVSEATRERVMQAAREFGYVADPTARRLATGRA
CCCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCC
DAVGIVYPFGAGDLGDPRFAEVVAGVTERLGESGLDFFIVSARPNAELDTYRRLVDGRLV
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHH
DGLIVARTLVDDPRLRFLQEREFPFVAYGRTASAKPYTWFDFDNEAGMHAAAQRLTAFGH
HHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHCC
RRIALVCAPRTLNFAAQRRAGFERALREAGIEPDAALIVECAFTRDGGYGAAQTLLALER
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHH
APSAIVVDNNIAGAGVFRAIVERGRRFVRDVSLIVYDGVSPDVSFPHHASAVLQPTGHAS
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCEECCCCCCC
GRAIAELMLGAIAHPGDAAHRLVSPVIEPGDTDGPFSG
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180920 [H]