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The map label for this gene is nemA [H]

Identifier: 126446179

GI number: 126446179

Start: 901816

End: 902877

Strand: Reverse

Name: nemA [H]

Synonym: BMA10247_A0951

Alternate gene names: 126446179

Gene position: 902877-901816 (Counterclockwise)

Preceding gene: 126447257

Following gene: 126447122

Centisome position: 38.38

GC content: 70.43

Gene sequence:

>1062_bases
ATGCCTTCTCTGTTCGATCCGCTGACCATCGGCGACCTCACACTGGCGAACCGCATCATCATGGCGCCGCTCACGCGCGC
CCGCGCCGGCGACACGCGCACGCCCAACGCGCTGATGGCGCGCTACTACGCGGAGCGCGCATCCGCGGGGCTCATCATCA
GCGAGGCGACGTCGGTCACGCCGCAAGGCGTCGGTTACGCAAGCACGCCGGGCATCTGGTCGCCCGAACAGGTCGACGGG
TGGCGACTCGTGACAGACGCGGTCCACGCGGCCGGCGGCCGCATCTTCCTGCAGCTCTGGCACGTCGGCCGCGTGTCGGA
TCCGGTGTTCCTCGACGGCGCGCTGCCCGTCGCGCCGAGCGCGATCGCCCCCGGCGGCCACGTGAGCCTCGTGCGCCCGC
AGCGGCCGTACGTGACGCCGCGCGCGCTCGAGCTCGACGAGATTCCGGGCGTCGTCGCCGCGTTCCGCCGCGGCGCGGAA
AACGCGCGCGCGGCCGGCTTCGACGGCGTCGAGGTACACGGCGCGAACGGCTATCTGCTCGATCAATTCCTGCAGGACAG
CGCGAACCGCCGCACCGACGCGTACGGCGGCTCGATCGAGAACCGCGCGCGCCTGTTGCTCGAGGTGGTCGACGCGGCGA
TCGACGTCTGGAGCGCGGCGCGCGTCGGCGTGCATCTCGCGCCGCGCGGCGATGCGCACACGATGGGCGATTCGGACCCC
GCGGCTACGTTCGGCCACGTCGCGCGCGAACTCGGCCGCCGCCGCATCGCGTTCCTGTTCGCACGCGAATCGTTCGGCGG
CGACGCCATCGGCCAGCAATTGAAGGCGGCGTTCGGCGGGCCATTCATCGTGAACGAGAATTTCACGCTCGACAGCGCGC
AGGCGGCGCTCGACGCGGGACAGGCGGACGCGGTCGCGTGGGGCAAGCTTTTTATCGCGAATCCGGACCTGCCGCGCCGC
TTCAAGCTGAATGCGCCGCTCAACGAGCCGAATGCCGCTACGTTCTATGCGCAAGGCGAAGTAGGCTATACGGACTATCC
GGCGCTGGAAAGCGCGGCGTGA

Upstream 100 bases:

>100_bases
AACGACGCGGTGATCCAGGCGTTTCTCGATTCGCTGCAGCGCGGCCTTTGATTCCGCCCACCCGCCGCAAGGGCTTAGCC
ACACTCGAAGAGGTTCCCTC

Downstream 100 bases:

>100_bases
GGAGGTGTCCGGACCCGGGCGCCGGCGTTTGGGACGGTTTGGTTTCGGCGGCTTGAGCCGTGCGCTTCGGGCGATTGGCG
ATCGGCGATCGGCCGTCATC

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MPSLFDPLTIGDLTLANRIIMAPLTRARAGDTRTPNALMARYYAERASAGLIISEATSVTPQGVGYASTPGIWSPEQVDG
WRLVTDAVHAAGGRIFLQLWHVGRVSDPVFLDGALPVAPSAIAPGGHVSLVRPQRPYVTPRALELDEIPGVVAAFRRGAE
NARAAGFDGVEVHGANGYLLDQFLQDSANRRTDAYGGSIENRARLLLEVVDAAIDVWSAARVGVHLAPRGDAHTMGDSDP
AATFGHVARELGRRRIAFLFARESFGGDAIGQQLKAAFGGPFIVNENFTLDSAQAALDAGQADAVAWGKLFIANPDLPRR
FKLNAPLNEPNAATFYAQGEVGYTDYPALESAA

Sequences:

>Translated_353_residues
MPSLFDPLTIGDLTLANRIIMAPLTRARAGDTRTPNALMARYYAERASAGLIISEATSVTPQGVGYASTPGIWSPEQVDG
WRLVTDAVHAAGGRIFLQLWHVGRVSDPVFLDGALPVAPSAIAPGGHVSLVRPQRPYVTPRALELDEIPGVVAAFRRGAE
NARAAGFDGVEVHGANGYLLDQFLQDSANRRTDAYGGSIENRARLLLEVVDAAIDVWSAARVGVHLAPRGDAHTMGDSDP
AATFGHVARELGRRRIAFLFARESFGGDAIGQQLKAAFGGPFIVNENFTLDSAQAALDAGQADAVAWGKLFIANPDLPRR
FKLNAPLNEPNAATFYAQGEVGYTDYPALESAA
>Mature_352_residues
PSLFDPLTIGDLTLANRIIMAPLTRARAGDTRTPNALMARYYAERASAGLIISEATSVTPQGVGYASTPGIWSPEQVDGW
RLVTDAVHAAGGRIFLQLWHVGRVSDPVFLDGALPVAPSAIAPGGHVSLVRPQRPYVTPRALELDEIPGVVAAFRRGAEN
ARAAGFDGVEVHGANGYLLDQFLQDSANRRTDAYGGSIENRARLLLEVVDAAIDVWSAARVGVHLAPRGDAHTMGDSDPA
ATFGHVARELGRRRIAFLFARESFGGDAIGQQLKAAFGGPFIVNENFTLDSAQAALDAGQADAVAWGKLFIANPDLPRRF
KLNAPLNEPNAATFYAQGEVGYTDYPALESAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=363, Percent_Identity=48.7603305785124, Blast_Score=307, Evalue=7e-85,
Organism=Escherichia coli, GI1789463, Length=215, Percent_Identity=39.5348837209302, Blast_Score=121, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17559804, Length=256, Percent_Identity=31.640625, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=255, Percent_Identity=29.0196078431373, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI72001454, Length=253, Percent_Identity=29.2490118577075, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=277, Percent_Identity=25.6317689530686, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=167, Percent_Identity=35.3293413173653, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17559802, Length=245, Percent_Identity=29.7959183673469, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17566914, Length=312, Percent_Identity=28.2051282051282, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=377, Percent_Identity=36.8700265251989, Blast_Score=208, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6321973, Length=377, Percent_Identity=37.1352785145889, Blast_Score=203, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 37539; Mature: 37407

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLFDPLTIGDLTLANRIIMAPLTRARAGDTRTPNALMARYYAERASAGLIISEATSVT
CCCCCCCCCCCHHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCC
PQGVGYASTPGIWSPEQVDGWRLVTDAVHAAGGRIFLQLWHVGRVSDPVFLDGALPVAPS
CCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCEEEEEEEECCCCCCCEEECCCCCCCCC
AIAPGGHVSLVRPQRPYVTPRALELDEIPGVVAAFRRGAENARAAGFDGVEVHGANGYLL
CCCCCCCEEEECCCCCCCCCCCCCHHHCCHHHHHHHCCCCCCCCCCCCCEEEECCCCHHH
DQFLQDSANRRTDAYGGSIENRARLLLEVVDAAIDVWSAARVGVHLAPRGDAHTMGDSDP
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCH
AATFGHVARELGRRRIAFLFARESFGGDAIGQQLKAAFGGPFIVNENFTLDSAQAALDAG
HHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHCCC
QADAVAWGKLFIANPDLPRRFKLNAPLNEPNAATFYAQGEVGYTDYPALESAA
CCCEEEEEEEEEECCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PSLFDPLTIGDLTLANRIIMAPLTRARAGDTRTPNALMARYYAERASAGLIISEATSVT
CCCCCCCCCCHHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCC
PQGVGYASTPGIWSPEQVDGWRLVTDAVHAAGGRIFLQLWHVGRVSDPVFLDGALPVAPS
CCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCEEEEEEEECCCCCCCEEECCCCCCCCC
AIAPGGHVSLVRPQRPYVTPRALELDEIPGVVAAFRRGAENARAAGFDGVEVHGANGYLL
CCCCCCCEEEECCCCCCCCCCCCCHHHCCHHHHHHHCCCCCCCCCCCCCEEEECCCCHHH
DQFLQDSANRRTDAYGGSIENRARLLLEVVDAAIDVWSAARVGVHLAPRGDAHTMGDSDP
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCH
AATFGHVARELGRRRIAFLFARESFGGDAIGQQLKAAFGGPFIVNENFTLDSAQAALDAG
HHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHCCC
QADAVAWGKLFIANPDLPRRFKLNAPLNEPNAATFYAQGEVGYTDYPALESAA
CCCEEEEEEEEEECCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]