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The map label for this gene is dctA [H]

Identifier: 126443302

GI number: 126443302

Start: 470984

End: 472225

Strand: Reverse

Name: dctA [H]

Synonym: BURPS668_A0518

Alternate gene names: 126443302

Gene position: 472225-470984 (Counterclockwise)

Preceding gene: 126443644

Following gene: 126445248

Centisome position: 15.1

GC content: 67.87

Gene sequence:

>1242_bases
ATGACACTGGCCAATAAAAAAATTTCGTTGCCGCTGCAAATGATCCTCGGCCTCGCGCTCGGCGTGGTCTTCGGCCTGCT
CGCGCCCGCCGCGAGCCGCGACCTGGCGTTCATTTCGACGCTGTTCGGCCACGCGATCAAGATGGTGGTGCTGCCGCTGA
TCCTGCTGTCGGTGACGCTCGGCGCGTTCCGCGCGGGCACGCAGCGCGGCCGGCTCGGCAAGACGGCCGCGTTCAGCCTC
GTGTTCTTCGTGCTGATGACGGTGATCGCCGCGGCGCTCGGCCTCGCGCTCAACTGGCTGTTCCGGCCCGGCATCGGCGC
GAGCCACGCGCAAACGGCATCGATGCCGGCGAACCTCGCGAGCGGCATCGACTGGATGAAGTTCCTGACCGACATGATCC
CGTCGAACATCGTCGGCGCGCTCGCGGCCGGCAATTCGCTGCCGGTGCTCGTGTTCGGCGTGCTGCTCGGCTGCGCGCTC
GCCGCGGTCGAGGACCGCGCGGCGCCGTTCGTCGCCGTCTGCGAATCGATGCTCGCCGCGTTCTTCAAGATGACCGAGTG
GGTCGTGTCGCTGTCCCCGATCGCGATCTTCGCCGCGATCGCGGTGCTGCTGTCGTCGAAGGGGCTCGCCGCGATGGCGC
CGCTCGCGAAGCTGCTCGGCATCGCGTACCTCGGCATGGCGCTGCTCGCCGCGTGGCTCACGCTGATCGTCAAGCTCGCC
GGCCATTCGCCGCGCGCGGTCGTGCGCAAGGTGAGCGAGCCGCTGATCCTCGGCTTCACGACGCGCTCGTCCGAAATCAC
GTTCCCCGTGCATCTGAAGAAGCTCACGGAAATGGGCGTGCCCTCCTCGGTCGCGTCGACCGTGCTGCCGCTGTCGTACA
TTTTCAATCGCGAAGGCGCGGTGCTCTACACGGTGCTCGCGGTCTGCTATCTCGCCGATGCGTATCAGCTCGCATGGAGC
TGGCCGCTGATGATCACCATCGCGGTCCTGACGATCATCACGATCGACGGCGCGGCCAACGTGCCTTCGGGCGCGGTGGT
CGCGATCACGGTGATCCTCGCCGCGATCGGATTGCCCGCCGATGCGGTGCTGCTGATTCTGGGCGTCGACGCGTTCTTCG
ACATGGGCCGCACCGCGCTGAACGTCTATGCGAGCACCGTCGCGACGACGCTCGCGAGCCGCGTGTCCGGCGCCGCGCCG
GAGATCGCGCAGGCGACGACGGCGCACGCATCGCGCGCGTGA

Upstream 100 bases:

>100_bases
GCGGCCATCGACGCGCGGGACGCGGAAACGGCGCGGCACCGGATGTGGAATCACGTGATCGACGGGACTCCGGACAGAAT
CCGATTAATTCGAGCTCAAT

Downstream 100 bases:

>100_bases
CGCCGCGCGGTTGCTCGCGCGGTTGAACATGTGCGCCGGCGTTGTCGGCGCGCACAACGGGATTGAAACCGGCGAGCGAA
TCCGGGAATTGAGACCGGCG

Product: dicarboxylate/amino acid:cation (Na+ or H+) symporter family protein

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTLANKKISLPLQMILGLALGVVFGLLAPAASRDLAFISTLFGHAIKMVVLPLILLSVTLGAFRAGTQRGRLGKTAAFSL
VFFVLMTVIAAALGLALNWLFRPGIGASHAQTASMPANLASGIDWMKFLTDMIPSNIVGALAAGNSLPVLVFGVLLGCAL
AAVEDRAAPFVAVCESMLAAFFKMTEWVVSLSPIAIFAAIAVLLSSKGLAAMAPLAKLLGIAYLGMALLAAWLTLIVKLA
GHSPRAVVRKVSEPLILGFTTRSSEITFPVHLKKLTEMGVPSSVASTVLPLSYIFNREGAVLYTVLAVCYLADAYQLAWS
WPLMITIAVLTIITIDGAANVPSGAVVAITVILAAIGLPADAVLLILGVDAFFDMGRTALNVYASTVATTLASRVSGAAP
EIAQATTAHASRA

Sequences:

>Translated_413_residues
MTLANKKISLPLQMILGLALGVVFGLLAPAASRDLAFISTLFGHAIKMVVLPLILLSVTLGAFRAGTQRGRLGKTAAFSL
VFFVLMTVIAAALGLALNWLFRPGIGASHAQTASMPANLASGIDWMKFLTDMIPSNIVGALAAGNSLPVLVFGVLLGCAL
AAVEDRAAPFVAVCESMLAAFFKMTEWVVSLSPIAIFAAIAVLLSSKGLAAMAPLAKLLGIAYLGMALLAAWLTLIVKLA
GHSPRAVVRKVSEPLILGFTTRSSEITFPVHLKKLTEMGVPSSVASTVLPLSYIFNREGAVLYTVLAVCYLADAYQLAWS
WPLMITIAVLTIITIDGAANVPSGAVVAITVILAAIGLPADAVLLILGVDAFFDMGRTALNVYASTVATTLASRVSGAAP
EIAQATTAHASRA
>Mature_412_residues
TLANKKISLPLQMILGLALGVVFGLLAPAASRDLAFISTLFGHAIKMVVLPLILLSVTLGAFRAGTQRGRLGKTAAFSLV
FFVLMTVIAAALGLALNWLFRPGIGASHAQTASMPANLASGIDWMKFLTDMIPSNIVGALAAGNSLPVLVFGVLLGCALA
AVEDRAAPFVAVCESMLAAFFKMTEWVVSLSPIAIFAAIAVLLSSKGLAAMAPLAKLLGIAYLGMALLAAWLTLIVKLAG
HSPRAVVRKVSEPLILGFTTRSSEITFPVHLKKLTEMGVPSSVASTVLPLSYIFNREGAVLYTVLAVCYLADAYQLAWSW
PLMITIAVLTIITIDGAANVPSGAVVAITVILAAIGLPADAVLLILGVDAFFDMGRTALNVYASTVATTLASRVSGAAPE
IAQATTAHASRA

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI5032093, Length=385, Percent_Identity=28.051948051948, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI169790839, Length=305, Percent_Identity=23.9344262295082, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI21314632, Length=426, Percent_Identity=24.4131455399061, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI4827012, Length=268, Percent_Identity=26.865671641791, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI194239697, Length=456, Percent_Identity=23.6842105263158, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI223468564, Length=278, Percent_Identity=28.0575539568345, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI223468566, Length=287, Percent_Identity=28.2229965156794, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI66773030, Length=392, Percent_Identity=23.7244897959184, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI40254478, Length=467, Percent_Identity=22.4839400428266, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1790514, Length=412, Percent_Identity=31.3106796116505, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1789947, Length=395, Percent_Identity=29.1139240506329, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788024, Length=401, Percent_Identity=26.9326683291771, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17537407, Length=454, Percent_Identity=24.6696035242291, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71996953, Length=450, Percent_Identity=27.3333333333333, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71983099, Length=439, Percent_Identity=25.7403189066059, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71983106, Length=439, Percent_Identity=25.7403189066059, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI193206654, Length=418, Percent_Identity=26.0765550239234, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI193206505, Length=449, Percent_Identity=22.71714922049, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17541374, Length=409, Percent_Identity=24.4498777506112, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24583025, Length=436, Percent_Identity=22.4770642201835, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17137668, Length=436, Percent_Identity=22.4770642201835, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24583023, Length=436, Percent_Identity=22.4770642201835, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 42869; Mature: 42738

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLANKKISLPLQMILGLALGVVFGLLAPAASRDLAFISTLFGHAIKMVVLPLILLSVTL
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAFRAGTQRGRLGKTAAFSLVFFVLMTVIAAALGLALNWLFRPGIGASHAQTASMPANLA
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHH
SGIDWMKFLTDMIPSNIVGALAAGNSLPVLVFGVLLGCALAAVEDRAAPFVAVCESMLAA
HHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
FFKMTEWVVSLSPIAIFAAIAVLLSSKGLAAMAPLAKLLGIAYLGMALLAAWLTLIVKLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GHSPRAVVRKVSEPLILGFTTRSSEITFPVHLKKLTEMGVPSSVASTVLPLSYIFNREGA
CCCHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCH
VLYTVLAVCYLADAYQLAWSWPLMITIAVLTIITIDGAANVPSGAVVAITVILAAIGLPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCH
DAVLLILGVDAFFDMGRTALNVYASTVATTLASRVSGAAPEIAQATTAHASRA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TLANKKISLPLQMILGLALGVVFGLLAPAASRDLAFISTLFGHAIKMVVLPLILLSVTL
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAFRAGTQRGRLGKTAAFSLVFFVLMTVIAAALGLALNWLFRPGIGASHAQTASMPANLA
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHH
SGIDWMKFLTDMIPSNIVGALAAGNSLPVLVFGVLLGCALAAVEDRAAPFVAVCESMLAA
HHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
FFKMTEWVVSLSPIAIFAAIAVLLSSKGLAAMAPLAKLLGIAYLGMALLAAWLTLIVKLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GHSPRAVVRKVSEPLILGFTTRSSEITFPVHLKKLTEMGVPSSVASTVLPLSYIFNREGA
CCCHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCH
VLYTVLAVCYLADAYQLAWSWPLMITIAVLTIITIDGAANVPSGAVVAITVILAAIGLPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCH
DAVLLILGVDAFFDMGRTALNVYASTVATTLASRVSGAAPEIAQATTAHASRA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]