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The map label for this gene is wecE [H]

Identifier: 126209013

GI number: 126209013

Start: 1771474

End: 1772619

Strand: Reverse

Name: wecE [H]

Synonym: APL_1549

Alternate gene names: 126209013

Gene position: 1772619-1771474 (Counterclockwise)

Preceding gene: 126209014

Following gene: 126209012

Centisome position: 77.94

GC content: 44.59

Gene sequence:

>1146_bases
ATGCAAATTCCTTTTAATAAACCGCCGATTGTCGGTACGGAACTCGGTTATATGCAACAAGCGATGGCAAGCGGGAAATT
ATCCGGCGACGGCTTTTATAGCCGGCGTTGCGAGCGGTGGCTGGAAAATCAATTCGGTACGGCAAAAGCATTGCTTACGC
CGTCTTGTACCGCTGCATTGGAAATGGCGGCGATATTAATCGATATTCAAGCCGGCGATGAAGTGATTATGCCGAGTTAT
ACCTTTGTTTCGACCGCTAACGCATTTGTGTTGCGTGGAGCAAAGATTGTGTTTGTCGATATTCGTCCCGATACGATGAA
TATTGATGAAACGAAAATTGAAGCGGCGATTACACCGAAAACCAAAGCGATTGTGCCGGTACATTACGCCGGTATCGCCT
GTGAAATGGATACGATTATGGCAATAGCCGAAAAATATCGGTTGTATGTGATCGAAGATGCGGCGCAAGCGGTAATGTCC
TCTTACAAAGGTAAAGCATTAGGCACTATTGGGCATTTCGGCTGTTATAGTTTTCACGAAACTAAGAATTATAGTGCCGG
CGGCGAAGGCGGTGCTTTATTGATTAATGATGATCGATTTATTGCTCGTGCGGAAGTGATCCGAGAAAAAGGCACAAATC
GTAGTCAATTCTTTCGTGGTGAAACGGATAAATACACTTGGCGAGATCTCGGATCCAGTTTTTTAATGTCGGAATTACAA
GCGGCCTATTTATATGCACAATTGGAAGTCGCACAACAAATCAAGCAGACCCGTTTAGCCGTTTGGCAACACTATTTTGA
AGTCTTACGTCCGTTTGCCGAACAAGGTCGTCTTGAGTTACCGACTTGCCCGCCCGAATGTGAACAAAACGGCCATCTGT
TTTATCTGAAATGGCGCAATTCGGATGAACGCAATGACTTTATCGAACGGCTAAAACAACACGGAATTTTAGCGGTATTT
CATTATGTGCCGTTACATAGCAGCCCTGCCGGTCAGCGGTTCGGCTCGTTTATCGGCGAAGACCGTTTTACTACTTCGGA
AAGCGAACGCTTGGTGCGTTTACCGCTATTTTATAATTTACAACCGCAAGAATCGGATAAAATTATTCGGGTTATATTGG
AATTTCTACATCAATGCAATCATTGA

Upstream 100 bases:

>100_bases
TTCGACTCAAATCGGTAACTTGCCTGCGATGAATCTCTATTTAACGCAGGGTGCGAGCATATTAGCGACGGCTTATTGGT
TTTATCGGAAATAATAATGT

Downstream 100 bases:

>100_bases
GCAAAACGTCCGTTTGGACGGCAAGCGCCACCGCATTTAAAATCGCTATTAGTTTAATACTGGTAAAGCTGTTTGCCTTG
CAATTCGGGTTGGAAGGCTT

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MQIPFNKPPIVGTELGYMQQAMASGKLSGDGFYSRRCERWLENQFGTAKALLTPSCTAALEMAAILIDIQAGDEVIMPSY
TFVSTANAFVLRGAKIVFVDIRPDTMNIDETKIEAAITPKTKAIVPVHYAGIACEMDTIMAIAEKYRLYVIEDAAQAVMS
SYKGKALGTIGHFGCYSFHETKNYSAGGEGGALLINDDRFIARAEVIREKGTNRSQFFRGETDKYTWRDLGSSFLMSELQ
AAYLYAQLEVAQQIKQTRLAVWQHYFEVLRPFAEQGRLELPTCPPECEQNGHLFYLKWRNSDERNDFIERLKQHGILAVF
HYVPLHSSPAGQRFGSFIGEDRFTTSESERLVRLPLFYNLQPQESDKIIRVILEFLHQCNH

Sequences:

>Translated_381_residues
MQIPFNKPPIVGTELGYMQQAMASGKLSGDGFYSRRCERWLENQFGTAKALLTPSCTAALEMAAILIDIQAGDEVIMPSY
TFVSTANAFVLRGAKIVFVDIRPDTMNIDETKIEAAITPKTKAIVPVHYAGIACEMDTIMAIAEKYRLYVIEDAAQAVMS
SYKGKALGTIGHFGCYSFHETKNYSAGGEGGALLINDDRFIARAEVIREKGTNRSQFFRGETDKYTWRDLGSSFLMSELQ
AAYLYAQLEVAQQIKQTRLAVWQHYFEVLRPFAEQGRLELPTCPPECEQNGHLFYLKWRNSDERNDFIERLKQHGILAVF
HYVPLHSSPAGQRFGSFIGEDRFTTSESERLVRLPLFYNLQPQESDKIIRVILEFLHQCNH
>Mature_381_residues
MQIPFNKPPIVGTELGYMQQAMASGKLSGDGFYSRRCERWLENQFGTAKALLTPSCTAALEMAAILIDIQAGDEVIMPSY
TFVSTANAFVLRGAKIVFVDIRPDTMNIDETKIEAAITPKTKAIVPVHYAGIACEMDTIMAIAEKYRLYVIEDAAQAVMS
SYKGKALGTIGHFGCYSFHETKNYSAGGEGGALLINDDRFIARAEVIREKGTNRSQFFRGETDKYTWRDLGSSFLMSELQ
AAYLYAQLEVAQQIKQTRLAVWQHYFEVLRPFAEQGRLELPTCPPECEQNGHLFYLKWRNSDERNDFIERLKQHGILAVF
HYVPLHSSPAGQRFGSFIGEDRFTTSESERLVRLPLFYNLQPQESDKIIRVILEFLHQCNH

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=374, Percent_Identity=71.1229946524064, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI145693159, Length=393, Percent_Identity=31.0432569974555, Blast_Score=164, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 43143; Mature: 43143

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIPFNKPPIVGTELGYMQQAMASGKLSGDGFYSRRCERWLENQFGTAKALLTPSCTAAL
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHH
EMAAILIDIQAGDEVIMPSYTFVSTANAFVLRGAKIVFVDIRPDTMNIDETKIEAAITPK
HHHHHEEEECCCCCEECCCCHHHHCCCEEEEECCEEEEEEECCCCCCCCHHHEEEEECCC
TKAIVPVHYAGIACEMDTIMAIAEKYRLYVIEDAAQAVMSSYKGKALGTIGHFGCYSFHE
CCEEEEEEECCEEEHHHHHHHHHHHHEEEEEHHHHHHHHHHCCCCEEECHHHHCCEEHHC
TKNYSAGGEGGALLINDDRFIARAEVIREKGTNRSQFFRGETDKYTWRDLGSSFLMSELQ
CCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHH
AAYLYAQLEVAQQIKQTRLAVWQHYFEVLRPFAEQGRLELPTCPPECEQNGHLFYLKWRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEECC
SDERNDFIERLKQHGILAVFHYVPLHSSPAGQRFGSFIGEDRFTTSESERLVRLPLFYNL
CCCHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECEEECC
QPQESDKIIRVILEFLHQCNH
CCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQIPFNKPPIVGTELGYMQQAMASGKLSGDGFYSRRCERWLENQFGTAKALLTPSCTAAL
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHH
EMAAILIDIQAGDEVIMPSYTFVSTANAFVLRGAKIVFVDIRPDTMNIDETKIEAAITPK
HHHHHEEEECCCCCEECCCCHHHHCCCEEEEECCEEEEEEECCCCCCCCHHHEEEEECCC
TKAIVPVHYAGIACEMDTIMAIAEKYRLYVIEDAAQAVMSSYKGKALGTIGHFGCYSFHE
CCEEEEEEECCEEEHHHHHHHHHHHHEEEEEHHHHHHHHHHCCCCEEECHHHHCCEEHHC
TKNYSAGGEGGALLINDDRFIARAEVIREKGTNRSQFFRGETDKYTWRDLGSSFLMSELQ
CCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHH
AAYLYAQLEVAQQIKQTRLAVWQHYFEVLRPFAEQGRLELPTCPPECEQNGHLFYLKWRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEECC
SDERNDFIERLKQHGILAVFHYVPLHSSPAGQRFGSFIGEDRFTTSESERLVRLPLFYNL
CCCHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECEEECC
QPQESDKIIRVILEFLHQCNH
CCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]