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The map label for this gene is mglB [H]

Identifier: 126207934

GI number: 126207934

Start: 518260

End: 519231

Strand: Direct

Name: mglB [H]

Synonym: APL_0450

Alternate gene names: 126207934

Gene position: 518260-519231 (Clockwise)

Preceding gene: 126207933

Following gene: 126207941

Centisome position: 22.79

GC content: 38.17

Gene sequence:

>972_bases
ATGAAAAAATTAGCAATATGTTCATTAGCCGTCGGGATAAGTTTGTCTTCTTCCGTTATCGCTCAAGATACGCTGGGTGT
GAGCTTATATCAGTACGACGATAATTTTATTCGATTAATGCATGATGAATTAATCTCATATACGTCAGAAAATCCTTCTC
TTTCTCTATTAATGAATGATGCGCAAAATTCGCAATTTGTGCAAAATAACCAAGTACAAACTTTAGTTAATCATAAAGTA
GATGTGCTTGCAGTAAATTTAGTTGATCCGAATGCATGGGCAACAGTGATCGGTAAGGCTAAAGATGCGAACTTACCGAT
TATCTTGTTCAATAAAGATCCGGGTGCGAAAGCAATCAATAGTTATAAAAAGGTTTATTATGTCGGGTCTAATCCGGAAG
AAGCAGGAAAAGCACAGGCTAAGATGATTGCCAATCATTGGAAAAGCAGTGAAGCGTTAGATTTAAATCACGACGGCAAA
ATGCAATATGCGGTATTAAAAGGGGAAGTAGGGCACCCGGATGCGGAAAAACGTACTAAAGCCGTAATTGAAGAATTAAA
AACACAGGGTATTAAGACGGAACAATTGGCATTAGAATCCGCCGTATGGAATAAAGAAATTGCGAAAGCTAAAGTAAACG
CTTGGTTATCGACACCGCAAAATAATATTGAAGTGATTATTTCTAATAACGATAGTATGGCGATAGGTGCGTTAGAGGTG
GTAACCGAAAAAGATAAAGCATTGCCTATTTACGGTGTGGATGCGACCCCAGAAGCACTTAATTTAATCAGAAAAGGAAA
ATTAGCCGGTACGGTCACCAACGATTGGCGTTCTCAAAGTAGAGCGATTATTGATTTTTCTCGCTTACTTGCAAAAGGGG
AATTACCGGAAAACAGTCAATGGAAACTTGAGAACAGAGTGTTGCGGATTCCGGCAATGTCTATTGATAGAAGCAATATT
AGCGATTTTTAA

Upstream 100 bases:

>100_bases
GTGAATTATCTCACATTATTTCCCTATTTAATTGTGTACTATCTTTTATATAGTAGTTCTAATTTAAAAGTCTTATTGAT
AATCTTGAGGGGGGAATAAT

Downstream 100 bases:

>100_bases
TACTATCTAAAAGATATTAAGGACTGAGGGATGGGATATTTATCGCAACTCAGTCCTTTCGTTTGAACTCAATTGCGGTC
ATCTTATCATTTTTGCAAAT

Product: D-galactose-binding periplasmic protein precursor

Products: ADP; phosphate; beta-D-galactose [Cytoplasm] [C]

Alternate protein names: GBP; D-galactose/ D-glucose-binding protein; GGBP [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKKLAICSLAVGISLSSSVIAQDTLGVSLYQYDDNFIRLMHDELISYTSENPSLSLLMNDAQNSQFVQNNQVQTLVNHKV
DVLAVNLVDPNAWATVIGKAKDANLPIILFNKDPGAKAINSYKKVYYVGSNPEEAGKAQAKMIANHWKSSEALDLNHDGK
MQYAVLKGEVGHPDAEKRTKAVIEELKTQGIKTEQLALESAVWNKEIAKAKVNAWLSTPQNNIEVIISNNDSMAIGALEV
VTEKDKALPIYGVDATPEALNLIRKGKLAGTVTNDWRSQSRAIIDFSRLLAKGELPENSQWKLENRVLRIPAMSIDRSNI
SDF

Sequences:

>Translated_323_residues
MKKLAICSLAVGISLSSSVIAQDTLGVSLYQYDDNFIRLMHDELISYTSENPSLSLLMNDAQNSQFVQNNQVQTLVNHKV
DVLAVNLVDPNAWATVIGKAKDANLPIILFNKDPGAKAINSYKKVYYVGSNPEEAGKAQAKMIANHWKSSEALDLNHDGK
MQYAVLKGEVGHPDAEKRTKAVIEELKTQGIKTEQLALESAVWNKEIAKAKVNAWLSTPQNNIEVIISNNDSMAIGALEV
VTEKDKALPIYGVDATPEALNLIRKGKLAGTVTNDWRSQSRAIIDFSRLLAKGELPENSQWKLENRVLRIPAMSIDRSNI
SDF
>Mature_323_residues
MKKLAICSLAVGISLSSSVIAQDTLGVSLYQYDDNFIRLMHDELISYTSENPSLSLLMNDAQNSQFVQNNQVQTLVNHKV
DVLAVNLVDPNAWATVIGKAKDANLPIILFNKDPGAKAINSYKKVYYVGSNPEEAGKAQAKMIANHWKSSEALDLNHDGK
MQYAVLKGEVGHPDAEKRTKAVIEELKTQGIKTEQLALESAVWNKEIAKAKVNAWLSTPQNNIEVIISNNDSMAIGALEV
VTEKDKALPIYGVDATPEALNLIRKGKLAGTVTNDWRSQSRAIIDFSRLLAKGELPENSQWKLENRVLRIPAMSIDRSNI
SDF

Specific function: This protein is involved in the active transport of galactose and glucose [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788473, Length=326, Percent_Identity=45.7055214723926, Blast_Score=310, Evalue=9e-86,
Organism=Escherichia coli, GI1790192, Length=275, Percent_Identity=24, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 4720 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35614; Mature: 35614

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLAICSLAVGISLSSSVIAQDTLGVSLYQYDDNFIRLMHDELISYTSENPSLSLLMND
CCCEEEEEEEECCCCCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEC
AQNSQFVQNNQVQTLVNHKVDVLAVNLVDPNAWATVIGKAKDANLPIILFNKDPGAKAIN
CCCCCCCCCCCHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCHHHHC
SYKKVYYVGSNPEEAGKAQAKMIANHWKSSEALDLNHDGKMQYAVLKGEVGHPDAEKRTK
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEEEECCCCCCCHHHHHH
AVIEELKTQGIKTEQLALESAVWNKEIAKAKVNAWLSTPQNNIEVIISNNDSMAIGALEV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEE
VTEKDKALPIYGVDATPEALNLIRKGKLAGTVTNDWRSQSRAIIDFSRLLAKGELPENSQ
EECCCCCCEEEECCCCHHHHHHHHCCCCCEEECCCHHHHCCHHHHHHHHHHCCCCCCCCC
WKLENRVLRIPAMSIDRSNISDF
EEECCEEEEECCEECCCCCCCCC
>Mature Secondary Structure
MKKLAICSLAVGISLSSSVIAQDTLGVSLYQYDDNFIRLMHDELISYTSENPSLSLLMND
CCCEEEEEEEECCCCCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEC
AQNSQFVQNNQVQTLVNHKVDVLAVNLVDPNAWATVIGKAKDANLPIILFNKDPGAKAIN
CCCCCCCCCCCHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCHHHHC
SYKKVYYVGSNPEEAGKAQAKMIANHWKSSEALDLNHDGKMQYAVLKGEVGHPDAEKRTK
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEEEECCCCCCCHHHHHH
AVIEELKTQGIKTEQLALESAVWNKEIAKAKVNAWLSTPQNNIEVIISNNDSMAIGALEV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEE
VTEKDKALPIYGVDATPEALNLIRKGKLAGTVTNDWRSQSRAIIDFSRLLAKGELPENSQ
EECCCCCCEEEECCCCHHHHHHHHCCCCCEEECCCHHHHCCHHHHHHHHHHCCCCCCCCC
WKLENRVLRIPAMSIDRSNISDF
EEECCEEEEECCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; beta-D-galactose [Periplasm]; H2O [C]

Specific reaction: ATP + beta-D-galactose [Periplasm] + H2O = ADP + phosphate + beta-D-galactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]