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The map label for this gene is purF [H]

Identifier: 126207909

GI number: 126207909

Start: 481684

End: 483201

Strand: Direct

Name: purF [H]

Synonym: APL_0425

Alternate gene names: 126207909

Gene position: 481684-483201 (Clockwise)

Preceding gene: 126207908

Following gene: 126207914

Centisome position: 21.18

GC content: 43.87

Gene sequence:

>1518_bases
ATGTGCGGCATTGTCGGCATTATTGGGGGATCGCCGGTTAATCAGGCGATTTATGATGGCTTAACATTACTTCAACATCG
CGGGCAAGATGCCGCAGGTATCGTCACAATTGATGATGAAAACCGCTTCCGCCTACGTAAAGCTAACGGCTTGGTAAGTG
ACGTATTTCGTCAAGAACATATGCTCAGATTACAAGGTAATGCGGGGATCGGACATGTTCGTTATCCGACCGCCGGCTCG
TCCAGTGTTTCGGAAGCACAACCTTTCTATGTAAACTCGCCTTTCGGTTTAACCTTAGTCCATAACGGCAACTTAACTAA
TAACGCCGAATTAAAAGAACGTTTATTCAAAGAAGCTCGTCGTCACGTGAATACCAATTCGGATTCGGAATCGTTACTGA
ATATTTTTGCGCATTATTTGGATCAGTATCCGACATCACATTTAACGCCGGAAAATATTTTTGAAACGGTACGTAAAACC
AACGAAGCGATTCGCGGTGCTTATGCGTGTATCGCGATGATTATCGGTCACGGTATGGTGGCATTCCGAGATCCGTTCGG
TATTCGTCCGCTAGTATTAGGTAAACGTGTTGTAGAAGGCAAAACGGAGTATATGTTTGCTTCCGAAAGCGTTGCTTTAG
ATATTGTCGGTTTTGAATTTGTCCGTGACGTAAATCCGGGCGAAGCGGTTTATATTACCTTCGACGGCGAATTACATTCC
GCAATTTGTGCGGATAATCCGAAATTAAACCCATGTATTTTTGAGTACGTTTATTTCGCTCGTCCGGATTCCGTGATTGA
CGGCGTATCCGTTTATGGTGCGCGTGTACATATGGGGGAATTATTAGGCGAAAAAATTAAACGCGAATGGGGCAGAATGG
TCGATGAAATTGATGTAGTGATTCCGATTCCTGAAACCTCAAACGATATTGCGGTACGCATCGCAAACGTTTTATACAAA
CCTTATCGCCAAGGTTTTGTGAAAAACCGCTATGTCGCTCGTACCTTTATTATGCCGGGACAAGCGCAGCGTAAAAGCTC
GGTACGCCGTAAATTAAATGCGATTGCCAGCGAGTTTAAAGGCAAAAACGTGTTATTAGTCGATGACTCTATCGTTCGCG
GAACAACATCCGAACAGATCGTTGAAATGGCACGTGCCGCTGGTGCGAAGAAAGTTTACTTCGCTTCAGCCGCACCGGAA
ATTCGTTACCCGAACGTATATGGTATTGATATGCCGACTTGTGAAGAGTTAGTGGCTTATGATCGCTCAGTAGAAGAAGT
GGCGGAAATGATTGGTGTAGATAAACTGATTTTCCAAGATCTCGAAGCATTATACAAAGCGGTTCAGGCAGAAAACCCAA
CAATTCAACATTTTGACGCATCAGTATTTACCGGCGAATATATTACCGGCGATGTAGATAAAGCTTACCTTGACGCAATT
GCACACGCTCGTAATGACAAAGCAAAAGCCAAAGCCGCAAAACAAGCAACCAATTTAGAAATTCATAACGAAAACTAA

Upstream 100 bases:

>100_bases
CAACTGATTCCGCATTTTGACTTTATTGTGAAATGGTTTTTTGAACAATTACAAGCTAATTCCAGCTTTTTAAATTCGAC
TAAATAGAGAGGTTTTTTAT

Downstream 100 bases:

>100_bases
ATTCAAGCATAAAAACAAGCCCTTGAGTCCGCGAGGATTCAAGGGCTTTTCTGTATGATATTTTTATCTTACTTATACAC
CTGCGGTATAGAGTATTCAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MCGIVGIIGGSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFRLRKANGLVSDVFRQEHMLRLQGNAGIGHVRYPTAGS
SSVSEAQPFYVNSPFGLTLVHNGNLTNNAELKERLFKEARRHVNTNSDSESLLNIFAHYLDQYPTSHLTPENIFETVRKT
NEAIRGAYACIAMIIGHGMVAFRDPFGIRPLVLGKRVVEGKTEYMFASESVALDIVGFEFVRDVNPGEAVYITFDGELHS
AICADNPKLNPCIFEYVYFARPDSVIDGVSVYGARVHMGELLGEKIKREWGRMVDEIDVVIPIPETSNDIAVRIANVLYK
PYRQGFVKNRYVARTFIMPGQAQRKSSVRRKLNAIASEFKGKNVLLVDDSIVRGTTSEQIVEMARAAGAKKVYFASAAPE
IRYPNVYGIDMPTCEELVAYDRSVEEVAEMIGVDKLIFQDLEALYKAVQAENPTIQHFDASVFTGEYITGDVDKAYLDAI
AHARNDKAKAKAAKQATNLEIHNEN

Sequences:

>Translated_505_residues
MCGIVGIIGGSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFRLRKANGLVSDVFRQEHMLRLQGNAGIGHVRYPTAGS
SSVSEAQPFYVNSPFGLTLVHNGNLTNNAELKERLFKEARRHVNTNSDSESLLNIFAHYLDQYPTSHLTPENIFETVRKT
NEAIRGAYACIAMIIGHGMVAFRDPFGIRPLVLGKRVVEGKTEYMFASESVALDIVGFEFVRDVNPGEAVYITFDGELHS
AICADNPKLNPCIFEYVYFARPDSVIDGVSVYGARVHMGELLGEKIKREWGRMVDEIDVVIPIPETSNDIAVRIANVLYK
PYRQGFVKNRYVARTFIMPGQAQRKSSVRRKLNAIASEFKGKNVLLVDDSIVRGTTSEQIVEMARAAGAKKVYFASAAPE
IRYPNVYGIDMPTCEELVAYDRSVEEVAEMIGVDKLIFQDLEALYKAVQAENPTIQHFDASVFTGEYITGDVDKAYLDAI
AHARNDKAKAKAAKQATNLEIHNEN
>Mature_505_residues
MCGIVGIIGGSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFRLRKANGLVSDVFRQEHMLRLQGNAGIGHVRYPTAGS
SSVSEAQPFYVNSPFGLTLVHNGNLTNNAELKERLFKEARRHVNTNSDSESLLNIFAHYLDQYPTSHLTPENIFETVRKT
NEAIRGAYACIAMIIGHGMVAFRDPFGIRPLVLGKRVVEGKTEYMFASESVALDIVGFEFVRDVNPGEAVYITFDGELHS
AICADNPKLNPCIFEYVYFARPDSVIDGVSVYGARVHMGELLGEKIKREWGRMVDEIDVVIPIPETSNDIAVRIANVLYK
PYRQGFVKNRYVARTFIMPGQAQRKSSVRRKLNAIASEFKGKNVLLVDDSIVRGTTSEQIVEMARAAGAKKVYFASAAPE
IRYPNVYGIDMPTCEELVAYDRSVEEVAEMIGVDKLIFQDLEALYKAVQAENPTIQHFDASVFTGEYITGDVDKAYLDAI
AHARNDKAKAKAAKQATNLEIHNEN

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=474, Percent_Identity=35.6540084388186, Blast_Score=255, Evalue=8e-68,
Organism=Escherichia coli, GI1788651, Length=506, Percent_Identity=70.5533596837945, Blast_Score=724, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=224, Percent_Identity=31.25, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=486, Percent_Identity=32.3045267489712, Blast_Score=221, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6323958, Length=503, Percent_Identity=50.4970178926441, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24659598, Length=500, Percent_Identity=35.6, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24659604, Length=476, Percent_Identity=35.9243697478992, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI28573187, Length=508, Percent_Identity=34.8425196850394, Blast_Score=254, Evalue=7e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56061; Mature: 56061

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIIGGSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFRLRKANGLVSDVFRQEH
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEHHCCHHHHHHHHHH
MLRLQGNAGIGHVRYPTAGSSSVSEAQPFYVNSPFGLTLVHNGNLTNNAELKERLFKEAR
EEEEECCCCCCEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHH
RHVNTNSDSESLLNIFAHYLDQYPTSHLTPENIFETVRKTNEAIRGAYACIAMIIGHGMV
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AFRDPFGIRPLVLGKRVVEGKTEYMFASESVALDIVGFEFVRDVNPGEAVYITFDGELHS
EECCCCCCCHHHHHHHHHCCCCCEEEECCCEEEEEECHHHHHCCCCCCEEEEEECCCCHH
AICADNPKLNPCIFEYVYFARPDSVIDGVSVYGARVHMGELLGEKIKREWGRMVDEIDVV
HHCCCCCCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
IPIPETSNDIAVRIANVLYKPYRQGFVKNRYVARTFIMPGQAQRKSSVRRKLNAIASEFK
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHC
GKNVLLVDDSIVRGTTSEQIVEMARAAGAKKVYFASAAPEIRYPNVYGIDMPTCEELVAY
CCEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECCCCCCHHHHHHH
DRSVEEVAEMIGVDKLIFQDLEALYKAVQAENPTIQHFDASVFTGEYITGDVDKAYLDAI
CCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEECCCEECCCHHHHHHHH
AHARNDKAKAKAAKQATNLEIHNEN
HHHCCCHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MCGIVGIIGGSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFRLRKANGLVSDVFRQEH
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEHHCCHHHHHHHHHH
MLRLQGNAGIGHVRYPTAGSSSVSEAQPFYVNSPFGLTLVHNGNLTNNAELKERLFKEAR
EEEEECCCCCCEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHH
RHVNTNSDSESLLNIFAHYLDQYPTSHLTPENIFETVRKTNEAIRGAYACIAMIIGHGMV
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AFRDPFGIRPLVLGKRVVEGKTEYMFASESVALDIVGFEFVRDVNPGEAVYITFDGELHS
EECCCCCCCHHHHHHHHHCCCCCEEEECCCEEEEEECHHHHHCCCCCCEEEEEECCCCHH
AICADNPKLNPCIFEYVYFARPDSVIDGVSVYGARVHMGELLGEKIKREWGRMVDEIDVV
HHCCCCCCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
IPIPETSNDIAVRIANVLYKPYRQGFVKNRYVARTFIMPGQAQRKSSVRRKLNAIASEFK
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHC
GKNVLLVDDSIVRGTTSEQIVEMARAAGAKKVYFASAAPEIRYPNVYGIDMPTCEELVAY
CCEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECCCCCCHHHHHHH
DRSVEEVAEMIGVDKLIFQDLEALYKAVQAENPTIQHFDASVFTGEYITGDVDKAYLDAI
CCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEECCCEECCCHHHHHHHH
AHARNDKAKAKAAKQATNLEIHNEN
HHHCCCHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10873488; 11248100 [H]