Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 126207836

GI number: 126207836

Start: 393700

End: 395727

Strand: Reverse

Name: metG [H]

Synonym: APL_0352

Alternate gene names: 126207836

Gene position: 395727-393700 (Counterclockwise)

Preceding gene: 126207840

Following gene: 126207835

Centisome position: 17.4

GC content: 41.07

Gene sequence:

>2028_bases
ATGCTGGTTACTTGTGCCTTGCCTTACGCTAACGGCGCAATTCATTTAGGTCATATGCTCGAACATATCCAAGCGGATAT
TTGGGTTCGCTTCCAACGTATGCGCGGTAACGAAATTTACTTTGTCTGTGCGGACGATGCGCACGGCACGCCGATTATGC
TTAATGCGGCAAAACAAGGTATTACGCCGGAACAATTAATTGAAAAAGCCAAAGCGGATCACGTTGCGGATTTCAAAGGC
TTTAATATCAGTTTCGATAACTATCATTCGACGCACAGTGAAGAAAACCGTGAAATTACCACGGAAATGTATAAAAAATT
ACGCGCTAACGGTTTTATCAAAAGTCGTGTGATTTCTCAGTTATTCGACCCGGAAAAACAAATGTTTCTTCCGGATCGTT
TTGTCAAAGGTACGTGCCCGAAATGTAAAGCGGAAGATCAATACGGTGATAACTGTGAAGTGTGCGCTTCAACTTATAGC
CCGATGGATTTAATTAATCCTCGCTCCGCCATATCCGGTGCCACACCAATCGTAAAAGAATCCGAACACTTTTTCTTTGA
TTTACCAAACTTTGAAGGGATGTTGAAAGAATGGACACGTTCAGGCTCATTACAATCGGAAATCGCCAATAAAATGCAAG
AATGGTTTGAAAGCGGTTTACAGCAATGGGATATCAGCCGTGATGCGCCTTACTTCGGTTTTCCGATTCCGGATGCGGAA
AATAAATTCTTCTACGTATGGCTTGATGCACCTATCGGCTATATGGCGTCATTTAAAAATTTATGTGATCGCACCGGTTT
AAACTTTGACGAATTCTGGAAAAAAGATTCAGAAACCGAGCTTTATCATTTTATCGGTAAAGATATCGTTTACTTCCACA
GTCTTTTCTGGCCGGCAATGCTGGACGGTTGTGAATTACGTAAGCCGACTAATGTATTTGCACACGGTTATGTGACGGTG
GACGGAGTGAAAATGTCCAAATCACGCGGTACCTTTATTCAAGCAAGCACCTATTTGAAACATATCGATCCGGAATGTTT
ACGCTATTACTATGCGGCAAAACTGAACGAACGTATTGAAGACTTGGATTTAAGCCTTGAAGATTTCGTACAACGTGTAA
ACAGCGATATCGTCAATAAATTAGTGAACCTCGCTTCACGTAATGCGAGTTTTATCGCCAAACGTTTTGAAGGTAAACTG
GCCGATAAATTAGAAGACGAAGCACTTTTTGCAGAATTTATCGCACAATCGGAGCAAATCGCCGCACATTATGAAAATCG
TGAATTTAACAAAGCGATTCGTTTAATTATGGATCTTTGCGATAAAGCGAATAAATATGTCGATGATAAAGCGCCTTGGG
TAATTGCAAAACAAGAAGGCTGTGATGCTCAATTACAAGCGGTATGTTCAATGGGTATCGAGTTATTCCGTGTGTTAATG
AGTTACTTAAAACCGGTATTACCGCAGTTAGCAGAACGTGCCGAAGCCTTCTTACAAACGGAATTAACCTGGGATAACAT
TCAGCAACCGTTATTAGGTCAAAATGTTGCGCCGTTTAAATCATTGTTCTCTCGTCTTGAGAAAAAACAAATTGATGCGG
TGATTGAAGAAACCAAAGCACTGTTTGCAGCACAAAATAAAGAAAAAGGAAAACAAAAAGTGGAAAATACTGAAAATACG
GCTGTGGAGCCTATCGCAGCGGAAATTACGATTGATGATTTCGCAAAATTAGATTTACGTGTGGCAAAAGTAATTAGCTG
TGAAGCCGTACCGGAATCAAACAAATTATTGAAATTCCAATTGGATTTAGGCGATCACCAACGCCAAGTATTATCCGGTA
TTAAAGCGGCTTACAATAATCCGGAAGAATTAGTCGGTCGTTTTGTGATTATGGTAGCAAACCTTGCACCACGTAAAATG
AAATTCGGCGTATCGGAAGGGATGATTTTATCCGCAGGCACAGGCGGTGCGGATTTATTCCTACTTTCAGCGGATGAAGG
TATCCGCCCGGGTATGCAAGTAAAATAA

Upstream 100 bases:

>100_bases
AAGCTAAAATTAAAAGAATATTTTTAAATTTCCTATTCCCCCTCTTTACACAAAAGGGGGATTTTTTCCGATTGCAGAGA
AACAAATAATGTCTAGAAAA

Downstream 100 bases:

>100_bases
TAGCTATAGTAAAAAAGGCGGTAAAAATTACCGCTTTTTTTGCGAACAAGGAGAAAAAATGATTCAACAAACACTCTGTT
TAATTAAACCCGATGCGACA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 675; Mature: 675

Protein sequence:

>675_residues
MLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNEIYFVCADDAHGTPIMLNAAKQGITPEQLIEKAKADHVADFKG
FNISFDNYHSTHSEENREITTEMYKKLRANGFIKSRVISQLFDPEKQMFLPDRFVKGTCPKCKAEDQYGDNCEVCASTYS
PMDLINPRSAISGATPIVKESEHFFFDLPNFEGMLKEWTRSGSLQSEIANKMQEWFESGLQQWDISRDAPYFGFPIPDAE
NKFFYVWLDAPIGYMASFKNLCDRTGLNFDEFWKKDSETELYHFIGKDIVYFHSLFWPAMLDGCELRKPTNVFAHGYVTV
DGVKMSKSRGTFIQASTYLKHIDPECLRYYYAAKLNERIEDLDLSLEDFVQRVNSDIVNKLVNLASRNASFIAKRFEGKL
ADKLEDEALFAEFIAQSEQIAAHYENREFNKAIRLIMDLCDKANKYVDDKAPWVIAKQEGCDAQLQAVCSMGIELFRVLM
SYLKPVLPQLAERAEAFLQTELTWDNIQQPLLGQNVAPFKSLFSRLEKKQIDAVIEETKALFAAQNKEKGKQKVENTENT
AVEPIAAEITIDDFAKLDLRVAKVISCEAVPESNKLLKFQLDLGDHQRQVLSGIKAAYNNPEELVGRFVIMVANLAPRKM
KFGVSEGMILSAGTGGADLFLLSADEGIRPGMQVK

Sequences:

>Translated_675_residues
MLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNEIYFVCADDAHGTPIMLNAAKQGITPEQLIEKAKADHVADFKG
FNISFDNYHSTHSEENREITTEMYKKLRANGFIKSRVISQLFDPEKQMFLPDRFVKGTCPKCKAEDQYGDNCEVCASTYS
PMDLINPRSAISGATPIVKESEHFFFDLPNFEGMLKEWTRSGSLQSEIANKMQEWFESGLQQWDISRDAPYFGFPIPDAE
NKFFYVWLDAPIGYMASFKNLCDRTGLNFDEFWKKDSETELYHFIGKDIVYFHSLFWPAMLDGCELRKPTNVFAHGYVTV
DGVKMSKSRGTFIQASTYLKHIDPECLRYYYAAKLNERIEDLDLSLEDFVQRVNSDIVNKLVNLASRNASFIAKRFEGKL
ADKLEDEALFAEFIAQSEQIAAHYENREFNKAIRLIMDLCDKANKYVDDKAPWVIAKQEGCDAQLQAVCSMGIELFRVLM
SYLKPVLPQLAERAEAFLQTELTWDNIQQPLLGQNVAPFKSLFSRLEKKQIDAVIEETKALFAAQNKEKGKQKVENTENT
AVEPIAAEITIDDFAKLDLRVAKVISCEAVPESNKLLKFQLDLGDHQRQVLSGIKAAYNNPEELVGRFVIMVANLAPRKM
KFGVSEGMILSAGTGGADLFLLSADEGIRPGMQVK
>Mature_675_residues
MLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNEIYFVCADDAHGTPIMLNAAKQGITPEQLIEKAKADHVADFKG
FNISFDNYHSTHSEENREITTEMYKKLRANGFIKSRVISQLFDPEKQMFLPDRFVKGTCPKCKAEDQYGDNCEVCASTYS
PMDLINPRSAISGATPIVKESEHFFFDLPNFEGMLKEWTRSGSLQSEIANKMQEWFESGLQQWDISRDAPYFGFPIPDAE
NKFFYVWLDAPIGYMASFKNLCDRTGLNFDEFWKKDSETELYHFIGKDIVYFHSLFWPAMLDGCELRKPTNVFAHGYVTV
DGVKMSKSRGTFIQASTYLKHIDPECLRYYYAAKLNERIEDLDLSLEDFVQRVNSDIVNKLVNLASRNASFIAKRFEGKL
ADKLEDEALFAEFIAQSEQIAAHYENREFNKAIRLIMDLCDKANKYVDDKAPWVIAKQEGCDAQLQAVCSMGIELFRVLM
SYLKPVLPQLAERAEAFLQTELTWDNIQQPLLGQNVAPFKSLFSRLEKKQIDAVIEETKALFAAQNKEKGKQKVENTENT
AVEPIAAEITIDDFAKLDLRVAKVISCEAVPESNKLLKFQLDLGDHQRQVLSGIKAAYNNPEELVGRFVIMVANLAPRKM
KFGVSEGMILSAGTGGADLFLLSADEGIRPGMQVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=627, Percent_Identity=28.7081339712919, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI24308436, Length=515, Percent_Identity=26.4077669902913, Blast_Score=173, Evalue=4e-43,
Organism=Escherichia coli, GI1788432, Length=677, Percent_Identity=67.3559822747415, Blast_Score=995, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=505, Percent_Identity=29.7029702970297, Blast_Score=254, Evalue=9e-68,
Organism=Caenorhabditis elegans, GI212645096, Length=513, Percent_Identity=25.1461988304094, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71992781, Length=407, Percent_Identity=25.3071253071253, Blast_Score=117, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321704, Length=565, Percent_Identity=29.0265486725664, Blast_Score=255, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6321610, Length=534, Percent_Identity=21.3483146067416, Blast_Score=113, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24655311, Length=557, Percent_Identity=29.6229802513465, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI21358483, Length=513, Percent_Identity=24.1715399610136, Blast_Score=152, Evalue=7e-37,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 76616; Mature: 76616

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNEIYFVCADDAHGTPIMLNAAKQG
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECHHCC
ITPEQLIEKAKADHVADFKGFNISFDNYHSTHSEENREITTEMYKKLRANGFIKSRVISQ
CCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
LFDPEKQMFLPDRFVKGTCPKCKAEDQYGDNCEVCASTYSPMDLINPRSAISGATPIVKE
HHCCHHHHCCCCHHHCCCCCCCCCCCCCCCCHHHHHCCCCCHHHCCCHHHHCCCCCCEEC
SEHFFFDLPNFEGMLKEWTRSGSLQSEIANKMQEWFESGLQQWDISRDAPYFGFPIPDAE
CCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
NKFFYVWLDAPIGYMASFKNLCDRTGLNFDEFWKKDSETELYHFIGKDIVYFHSLFWPAM
CCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDGCELRKPTNVFAHGYVTVDGVKMSKSRGTFIQASTYLKHIDPECLRYYYAAKLNERIE
HCCCCCCCCCCEEEECEEEEECEEECCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHH
DLDLSLEDFVQRVNSDIVNKLVNLASRNASFIAKRFEGKLADKLEDEALFAEFIAQSEQI
HHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AAHYENREFNKAIRLIMDLCDKANKYVDDKAPWVIAKQEGCDAQLQAVCSMGIELFRVLM
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYLKPVLPQLAERAEAFLQTELTWDNIQQPLLGQNVAPFKSLFSRLEKKQIDAVIEETKA
HHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LFAAQNKEKGKQKVENTENTAVEPIAAEITIDDFAKLDLRVAKVISCEAVPESNKLLKFQ
HHHHCCHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
LDLGDHQRQVLSGIKAAYNNPEELVGRFVIMVANLAPRKMKFGVSEGMILSAGTGGADLF
ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEECCCCCCEEE
LLSADEGIRPGMQVK
EEECCCCCCCCCCCC
>Mature Secondary Structure
MLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNEIYFVCADDAHGTPIMLNAAKQG
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECHHCC
ITPEQLIEKAKADHVADFKGFNISFDNYHSTHSEENREITTEMYKKLRANGFIKSRVISQ
CCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
LFDPEKQMFLPDRFVKGTCPKCKAEDQYGDNCEVCASTYSPMDLINPRSAISGATPIVKE
HHCCHHHHCCCCHHHCCCCCCCCCCCCCCCCHHHHHCCCCCHHHCCCHHHHCCCCCCEEC
SEHFFFDLPNFEGMLKEWTRSGSLQSEIANKMQEWFESGLQQWDISRDAPYFGFPIPDAE
CCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
NKFFYVWLDAPIGYMASFKNLCDRTGLNFDEFWKKDSETELYHFIGKDIVYFHSLFWPAM
CCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDGCELRKPTNVFAHGYVTVDGVKMSKSRGTFIQASTYLKHIDPECLRYYYAAKLNERIE
HCCCCCCCCCCEEEECEEEEECEEECCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHH
DLDLSLEDFVQRVNSDIVNKLVNLASRNASFIAKRFEGKLADKLEDEALFAEFIAQSEQI
HHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AAHYENREFNKAIRLIMDLCDKANKYVDDKAPWVIAKQEGCDAQLQAVCSMGIELFRVLM
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYLKPVLPQLAERAEAFLQTELTWDNIQQPLLGQNVAPFKSLFSRLEKKQIDAVIEETKA
HHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LFAAQNKEKGKQKVENTENTAVEPIAAEITIDDFAKLDLRVAKVISCEAVPESNKLLKFQ
HHHHCCHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
LDLGDHQRQVLSGIKAAYNNPEELVGRFVIMVANLAPRKMKFGVSEGMILSAGTGGADLF
ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEECCCCCCEEE
LLSADEGIRPGMQVK
EEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA