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The map label for this gene is nhaB

Identifier: 126207806

GI number: 126207806

Start: 358750

End: 360291

Strand: Reverse

Name: nhaB

Synonym: APL_0322

Alternate gene names: 126207806

Gene position: 360291-358750 (Counterclockwise)

Preceding gene: 126207809

Following gene: 126207805

Centisome position: 15.84

GC content: 40.92

Gene sequence:

>1542_bases
ATGGATAGCTCGAACGCTATATTTAAAAGCTTTTTAGGTAAAGCCCCCGAATGGTACAAAATCTGTATTATCGCTTTTTT
AGTTATTAATCCGTTAATTTACTTTTTTATTAGCCCTTTCGTTGCCGGCTGGGCATTGGTCGCAGAATTCATCTTTACCT
TATCCATGGCATTAAAATGCTATCCGTTACAACCCGGCGGTCTGTTAGCAATTGAAGCGGTTTTCATCGGGATGACCTCC
GCTCATCACGTTAAAGAAGAGATCATGGCTAACTTTGAAGTAATTTTATTATTAATGTTCATGGTTGCCGGTATTTATTT
TATGAAGCAATTGCTTTTATATGTATTTACCAAATTGTTAATTGTTATCCATTCTAAGAAAATTCTTTCTCTTGCGTTTT
GTTTAAGTGCGACCTTCCTCTCCGCATTCTTAGACGCATTAACCGTAATTGCCGTAATTATTAGTGTCGGTACCGGTTTC
TACGGTGTTTATCATAAAGTCGCTTCCGGTAATAGTTTCGAAGATTCGACGGATATTAGTAATGACGAAAAAATTATTAC
GAATAAAGAAATTCTCGAACAATTCCGTGCCTTCTTACGTAGTCCTTTAATGCACGCTGCCGTCGGTAGTGCTTTAGGCG
GTGTAATGACCATGGTTGGCGAGCCGCAAAACTTAATTATCGCCGGTCAAGCGGAATGGGGCTTCGTTGAATTCTTACTA
CGCGTATTGCCTGTTAGTTTACCGGTTTTAATTTGCGGTGTAATTACTTGTTTATTGTTAGAACACTTTAAAATTTTCGG
TTATGGAGCGCGTTTACCGCGCCGCGTATGGGGCGTACTTGCTCGTTATAACTTATTAAAAGAACAACGTATGACTCAAC
AAGATCGTGTAAAAATGGGCATCCAAGCGTTAGCGGGGATTTGGTTGGTTGTCGGTCTGGCGCTTCATCTTGCCGATGTC
GGTATTATCGGTTTAACCATTATCATTATCTGTACGGCGTTTTGCGGTATTACCGATGAACATGCAATCGGTCGTTCATT
CCAAGAACCGATGCCGTTTACCGCGTTAATCGTAGTCTTCTTTACGGTTGTTGCGGTCATTGTCGATCTGAAATTATTCG
AGCCGATTATCAGTTACGTGCTTTCTGCCGATCCTCATTCTCAATTGGCATTGTTCTATGTATTTAACGGCTTATTATCG
ATGATTTCGGATAACGTATTCGTCGGTACGGTGTATATTAACGAAGCGAAAACCGCACTCGAATCGGCAATTATTAGTCG
TGAACAATTCGATTTAATTGCGGTGGCAATCAATACCGGCACGAACTTACCGTCTGTCGCAACACCAAACGGACAAGCGG
CGTTCCTATTCTTGCTTACTTCACCGTTTGCACCGTTAATCCGACTTTCATACGGTAAAATGTTATATATGGCATTGCCT
TATACGATCGTGCTTTCTATCATCGGCTTCTTATCATTAGAGTTTTTACTCCCGCCGCTTACAGAGTTAATGTCTAACTG
GGGTTGGATCATTACTCGATAA

Upstream 100 bases:

>100_bases
AATCAAAAAAAATCTCGTAAAAAATGAGATAAAGGCTATTATCCTTTAATCTAAACTGGATTACAATTAACAAAACTTAT
TATTTTAATGGAGGTTCTTA

Downstream 100 bases:

>100_bases
TTCAAAGAATCTCCCCTAAAAGTGGGGAGATTTTTTTACGAAAACCGAACAAAATCAGGTACAATGTAAAAATTATTCAG
ACCAGAGGTGCCTATTATGC

Product: sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaB

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MDSSNAIFKSFLGKAPEWYKICIIAFLVINPLIYFFISPFVAGWALVAEFIFTLSMALKCYPLQPGGLLAIEAVFIGMTS
AHHVKEEIMANFEVILLLMFMVAGIYFMKQLLLYVFTKLLIVIHSKKILSLAFCLSATFLSAFLDALTVIAVIISVGTGF
YGVYHKVASGNSFEDSTDISNDEKIITNKEILEQFRAFLRSPLMHAAVGSALGGVMTMVGEPQNLIIAGQAEWGFVEFLL
RVLPVSLPVLICGVITCLLLEHFKIFGYGARLPRRVWGVLARYNLLKEQRMTQQDRVKMGIQALAGIWLVVGLALHLADV
GIIGLTIIIICTAFCGITDEHAIGRSFQEPMPFTALIVVFFTVVAVIVDLKLFEPIISYVLSADPHSQLALFYVFNGLLS
MISDNVFVGTVYINEAKTALESAIISREQFDLIAVAINTGTNLPSVATPNGQAAFLFLLTSPFAPLIRLSYGKMLYMALP
YTIVLSIIGFLSLEFLLPPLTELMSNWGWIITR

Sequences:

>Translated_513_residues
MDSSNAIFKSFLGKAPEWYKICIIAFLVINPLIYFFISPFVAGWALVAEFIFTLSMALKCYPLQPGGLLAIEAVFIGMTS
AHHVKEEIMANFEVILLLMFMVAGIYFMKQLLLYVFTKLLIVIHSKKILSLAFCLSATFLSAFLDALTVIAVIISVGTGF
YGVYHKVASGNSFEDSTDISNDEKIITNKEILEQFRAFLRSPLMHAAVGSALGGVMTMVGEPQNLIIAGQAEWGFVEFLL
RVLPVSLPVLICGVITCLLLEHFKIFGYGARLPRRVWGVLARYNLLKEQRMTQQDRVKMGIQALAGIWLVVGLALHLADV
GIIGLTIIIICTAFCGITDEHAIGRSFQEPMPFTALIVVFFTVVAVIVDLKLFEPIISYVLSADPHSQLALFYVFNGLLS
MISDNVFVGTVYINEAKTALESAIISREQFDLIAVAINTGTNLPSVATPNGQAAFLFLLTSPFAPLIRLSYGKMLYMALP
YTIVLSIIGFLSLEFLLPPLTELMSNWGWIITR
>Mature_513_residues
MDSSNAIFKSFLGKAPEWYKICIIAFLVINPLIYFFISPFVAGWALVAEFIFTLSMALKCYPLQPGGLLAIEAVFIGMTS
AHHVKEEIMANFEVILLLMFMVAGIYFMKQLLLYVFTKLLIVIHSKKILSLAFCLSATFLSAFLDALTVIAVIISVGTGF
YGVYHKVASGNSFEDSTDISNDEKIITNKEILEQFRAFLRSPLMHAAVGSALGGVMTMVGEPQNLIIAGQAEWGFVEFLL
RVLPVSLPVLICGVITCLLLEHFKIFGYGARLPRRVWGVLARYNLLKEQRMTQQDRVKMGIQALAGIWLVVGLALHLADV
GIIGLTIIIICTAFCGITDEHAIGRSFQEPMPFTALIVVFFTVVAVIVDLKLFEPIISYVLSADPHSQLALFYVFNGLLS
MISDNVFVGTVYINEAKTALESAIISREQFDLIAVAINTGTNLPSVATPNGQAAFLFLLTSPFAPLIRLSYGKMLYMALP
YTIVLSIIGFLSLEFLLPPLTELMSNWGWIITR

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3067

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaB Na(+)/H(+) (TC 2.A.34) antiporter family

Homologues:

Organism=Escherichia coli, GI1787435, Length=509, Percent_Identity=65.4223968565815, Blast_Score=665, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAB_ACTP2 (A3MZ40)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053031.1
- STRING:   A3MZ40
- GeneID:   4849254
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0322
- eggNOG:   COG3067
- HOGENOM:   HBG296776
- OMA:   CFLVEKF
- PhylomeDB:   A3MZ40
- ProtClustDB:   PRK09547
- BioCyc:   APLE416269:APL_0322-MONOMER
- HAMAP:   MF_01599
- InterPro:   IPR004671
- TIGRFAMs:   TIGR00774

Pfam domain/function: PF06450 NhaB

EC number: NA

Molecular weight: Translated: 56633; Mature: 56633

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b48c8b0)-; HASH(0x1bc5839c)-; HASH(0x1c99e15c)-; HASH(0x1ae4a320)-; HASH(0x1c73d130)-; HASH(0x1c8753bc)-; HASH(0x1b42fd9c)-; HASH(0x1c773fc0)-; HASH(0x1b433f1c)-;

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSSNAIFKSFLGKAPEWYKICIIAFLVINPLIYFFISPFVAGWALVAEFIFTLSMALKC
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
YPLQPGGLLAIEAVFIGMTSAHHVKEEIMANFEVILLLMFMVAGIYFMKQLLLYVFTKLL
ECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVIHSKKILSLAFCLSATFLSAFLDALTVIAVIISVGTGFYGVYHKVASGNSFEDSTDIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCC
NDEKIITNKEILEQFRAFLRSPLMHAAVGSALGGVMTMVGEPQNLIIAGQAEWGFVEFLL
CCCCEECCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHH
RVLPVSLPVLICGVITCLLLEHFKIFGYGARLPRRVWGVLARYNLLKEQRMTQQDRVKMG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQALAGIWLVVGLALHLADVGIIGLTIIIICTAFCGITDEHAIGRSFQEPMPFTALIVVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHH
FTVVAVIVDLKLFEPIISYVLSADPHSQLALFYVFNGLLSMISDNVFVGTVYINEAKTAL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHH
ESAIISREQFDLIAVAINTGTNLPSVATPNGQAAFLFLLTSPFAPLIRLSYGKMLYMALP
HHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
YTIVLSIIGFLSLEFLLPPLTELMSNWGWIITR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEH
>Mature Secondary Structure
MDSSNAIFKSFLGKAPEWYKICIIAFLVINPLIYFFISPFVAGWALVAEFIFTLSMALKC
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
YPLQPGGLLAIEAVFIGMTSAHHVKEEIMANFEVILLLMFMVAGIYFMKQLLLYVFTKLL
ECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVIHSKKILSLAFCLSATFLSAFLDALTVIAVIISVGTGFYGVYHKVASGNSFEDSTDIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCC
NDEKIITNKEILEQFRAFLRSPLMHAAVGSALGGVMTMVGEPQNLIIAGQAEWGFVEFLL
CCCCEECCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHH
RVLPVSLPVLICGVITCLLLEHFKIFGYGARLPRRVWGVLARYNLLKEQRMTQQDRVKMG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQALAGIWLVVGLALHLADVGIIGLTIIIICTAFCGITDEHAIGRSFQEPMPFTALIVVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHH
FTVVAVIVDLKLFEPIISYVLSADPHSQLALFYVFNGLLSMISDNVFVGTVYINEAKTAL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHH
ESAIISREQFDLIAVAINTGTNLPSVATPNGQAAFLFLLTSPFAPLIRLSYGKMLYMALP
HHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
YTIVLSIIGFLSLEFLLPPLTELMSNWGWIITR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3 Proton [Periplasm]; Na (I) [Cytoplasm] [C]

Specific reaction: 3 Proton [Periplasm] + 2 Na (I) [Cytoplasm] = 2 Na (I) [Periplasm] + 3 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA