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The map label for this gene is dusC [H]

Identifier: 126207791

GI number: 126207791

Start: 339383

End: 340345

Strand: Direct

Name: dusC [H]

Synonym: APL_0307

Alternate gene names: 126207791

Gene position: 339383-340345 (Clockwise)

Preceding gene: 126207790

Following gene: 126207792

Centisome position: 14.92

GC content: 44.55

Gene sequence:

>963_bases
ATGATAAATAATATACCCAAAGTGATTTTAGCACCGATGCAAGGTGTGCTTGATCCTTTTGTCCGTAAATTACTCACTGC
CGTAAATGATTACGATCTCTGTATTTCCGAATTTGTACGAGTGGTGGATCAAAAATTGCCGAAGAAAGCGTTTTATCGCC
GTGCCCCTGAATTGTTGCAAGGCGGTTTAACCGATTCAGGAACGCCTGTGAGAGTGCAGCTATTGGGGCAACATCCGCAA
TGGCTGGCGGAGAATGCCCGCTTGGCGATAGAGTTGGGATCGCAGGGAGTGGATTTGAATTGCGGTTGTCCGTCTAAAAC
GGTAAACGGTAGTAATGGCGGCGCATCATTGTTAAAAGATCCGGATTTAATTTATCGTGCAACCAAAGCAATGCGAGAAG
CGGTTCCACAGGATCAGATCGTTTCGGTGAAAGTACGTTTGGGCTGGGATTCTGCGGATCAAAGTTTTGAAATTGCCGAT
GCGGTGGCACAAGGCGGTGCAAATGAAATTACCGTACACGGTCGCACTAAACAAGACGGCTATCGAGCGGAACGAATTAA
TTGGCAGGCAATCGGCGAAATTCAAAAACGCCTTTCGATTCCTGTGATCGCCAATGGTGAAATTTGGGATTTTGAATCGG
CAAAAAACTGTCAAAATACGACCGCTTGTTCGAGCCTTATGATTGGACGAGGCGCATTAAACACACCGAACCTCAGCCGA
GTGGTGAAATTTAATCAGCCAAAAATGGCGTGGAATGAAGTGCTGCAATTACTGTTTCAATATGTGAATATGGAAAATGA
GCATGACTCGGGCTTTTATCATGTGGCGAGAATTAAGCAATGGCTACATTATTTGGATAAAGAATATCCGCAAGCGAAAG
ATTTGTTCCAGATACTGAAAACGGAACACGGCTATGACGGTCTAAAAACGCATATTGAACGAGCGGTAAATCAAGAGTTT
TAA

Upstream 100 bases:

>100_bases
GAAAGGTTGGAAATAATAAAAACATCTCAAGCGGTCGTTTTTGGTAAAAATTTTGCAAAAATCGACCGCTTACTTTTTGA
ATCGAATAAGTAAAAATTGA

Downstream 100 bases:

>100_bases
CCACGGAAAACACTATTAGCATTATCCGTGCTTAATTGAAAAGAGAAAATGATGAAGAAACAAATATTTAGTGAGCAAGA
GATCACAACAGAGCAGCAAT

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MINNIPKVILAPMQGVLDPFVRKLLTAVNDYDLCISEFVRVVDQKLPKKAFYRRAPELLQGGLTDSGTPVRVQLLGQHPQ
WLAENARLAIELGSQGVDLNCGCPSKTVNGSNGGASLLKDPDLIYRATKAMREAVPQDQIVSVKVRLGWDSADQSFEIAD
AVAQGGANEITVHGRTKQDGYRAERINWQAIGEIQKRLSIPVIANGEIWDFESAKNCQNTTACSSLMIGRGALNTPNLSR
VVKFNQPKMAWNEVLQLLFQYVNMENEHDSGFYHVARIKQWLHYLDKEYPQAKDLFQILKTEHGYDGLKTHIERAVNQEF

Sequences:

>Translated_320_residues
MINNIPKVILAPMQGVLDPFVRKLLTAVNDYDLCISEFVRVVDQKLPKKAFYRRAPELLQGGLTDSGTPVRVQLLGQHPQ
WLAENARLAIELGSQGVDLNCGCPSKTVNGSNGGASLLKDPDLIYRATKAMREAVPQDQIVSVKVRLGWDSADQSFEIAD
AVAQGGANEITVHGRTKQDGYRAERINWQAIGEIQKRLSIPVIANGEIWDFESAKNCQNTTACSSLMIGRGALNTPNLSR
VVKFNQPKMAWNEVLQLLFQYVNMENEHDSGFYHVARIKQWLHYLDKEYPQAKDLFQILKTEHGYDGLKTHIERAVNQEF
>Mature_320_residues
MINNIPKVILAPMQGVLDPFVRKLLTAVNDYDLCISEFVRVVDQKLPKKAFYRRAPELLQGGLTDSGTPVRVQLLGQHPQ
WLAENARLAIELGSQGVDLNCGCPSKTVNGSNGGASLLKDPDLIYRATKAMREAVPQDQIVSVKVRLGWDSADQSFEIAD
AVAQGGANEITVHGRTKQDGYRAERINWQAIGEIQKRLSIPVIANGEIWDFESAKNCQNTTACSSLMIGRGALNTPNLSR
VVKFNQPKMAWNEVLQLLFQYVNMENEHDSGFYHVARIKQWLHYLDKEYPQAKDLFQILKTEHGYDGLKTHIERAVNQEF

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=247, Percent_Identity=29.9595141700405, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI40807366, Length=268, Percent_Identity=28.3582089552239, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI239788483, Length=228, Percent_Identity=28.0701754385965, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI8923374, Length=198, Percent_Identity=27.7777777777778, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI239788462, Length=228, Percent_Identity=28.0701754385965, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788462, Length=295, Percent_Identity=68.4745762711864, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1789660, Length=295, Percent_Identity=30.8474576271186, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI145693211, Length=260, Percent_Identity=25.3846153846154, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=31.6279069767442, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=178, Percent_Identity=28.6516853932584, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=249, Percent_Identity=26.5060240963855, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=146, Percent_Identity=28.0821917808219, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19921524, Length=172, Percent_Identity=29.6511627906977, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24585320, Length=239, Percent_Identity=30.9623430962343, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24580595, Length=293, Percent_Identity=24.2320819112628, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19920448, Length=293, Percent_Identity=24.2320819112628, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35888; Mature: 35888

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINNIPKVILAPMQGVLDPFVRKLLTAVNDYDLCISEFVRVVDQKLPKKAFYRRAPELLQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHCHHHHH
GGLTDSGTPVRVQLLGQHPQWLAENARLAIELGSQGVDLNCGCPSKTVNGSNGGASLLKD
CCCCCCCCCEEEEEECCCCHHHHCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCHHHCC
PDLIYRATKAMREAVPQDQIVSVKVRLGWDSADQSFEIADAVAQGGANEITVHGRTKQDG
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCC
YRAERINWQAIGEIQKRLSIPVIANGEIWDFESAKNCQNTTACSSLMIGRGALNTPNLSR
CHHHHCCHHHHHHHHHHHCCCEEECCCEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
VVKFNQPKMAWNEVLQLLFQYVNMENEHDSGFYHVARIKQWLHYLDKEYPQAKDLFQILK
HEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TEHGYDGLKTHIERAVNQEF
HCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MINNIPKVILAPMQGVLDPFVRKLLTAVNDYDLCISEFVRVVDQKLPKKAFYRRAPELLQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHCHHHHH
GGLTDSGTPVRVQLLGQHPQWLAENARLAIELGSQGVDLNCGCPSKTVNGSNGGASLLKD
CCCCCCCCCEEEEEECCCCHHHHCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCHHHCC
PDLIYRATKAMREAVPQDQIVSVKVRLGWDSADQSFEIADAVAQGGANEITVHGRTKQDG
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCC
YRAERINWQAIGEIQKRLSIPVIANGEIWDFESAKNCQNTTACSSLMIGRGALNTPNLSR
CHHHHCCHHHHHHHHHHHCCCEEECCCEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
VVKFNQPKMAWNEVLQLLFQYVNMENEHDSGFYHVARIKQWLHYLDKEYPQAKDLFQILK
HEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TEHGYDGLKTHIERAVNQEF
HCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA