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The map label for this gene is rep [H]

Identifier: 126207767

GI number: 126207767

Start: 307806

End: 309824

Strand: Reverse

Name: rep [H]

Synonym: APL_0279

Alternate gene names: 126207767

Gene position: 309824-307806 (Counterclockwise)

Preceding gene: 126207773

Following gene: 126207762

Centisome position: 13.62

GC content: 42.25

Gene sequence:

>2019_bases
ATGAAATTAAATGATCAACAACAACAAGCGGTGGAATATGTAACAGGAGCTTGCCTTGTGTTAGCGGGTGCAGGCTCGGG
CAAAACACGTGTCATCATTAATAAAATCGCTCATCTGATTGCGCATTGCGGTTATTCACCGAAGCAAATCGCAGCGGTAA
CCTTTACCAATAAAGCGGCACGTGAAATGCGTGAGCGAGTTGCCCATTCCATCGGTAAAGAAAACAGCAAAGGGCTAACC
ATTTCGACTTTCCACACGCTTGGTTTTGAAATTTTAAAACGTGAGTATAAATTACTGGGTTTCAAATCCGGTATGACGTT
GTTTGATGAACACGATCAACTCGCATTACTCAAACATTTGTTACCGGAAAATGCGGCGGAAGATAAAGATTTACTCAAAG
CATTGATTTCTACTATTTCCAACTGGAAAAACGATCTGCTTTCGCCCGAAATGGTATTAACGCGTGTGCGAGATGAGCGC
GAGCGTGTGTTTTCGCATTTTTATCAGCTCTACCAAAATCAGCTAAAAGCCTATAATGCGCTTGATTTTGACGATTTAAT
TATGCTGCCGGTATTGCTGTTCCGCCAATTTCCGGAAGCGAAAACCCGTTGGCAGCAGAAAGTCCGTTATTTACTGGTGG
ACGAATACCAAGATACCAATACCAGCCAATATGAGTTGATCAAATTATTGGTAGGCAATGAAGCCAATTTCACTGTGGTA
GGTGATGACGACCAATCAATCTATTCGTGGCGTGGCGCAAAACCGGAAAATATGCAACGTTTACGTGAAGATTTTGATTT
GAAAGTGATTAAACTAGAACAAAATTACCGTTCCAGCCGACGTATTCTGCATTGTGCCAATATTTTGATTGATAACAACG
ATCATATTTTCCAAAAACGCTTATTTTCGAATTTAGGCGAAGGCGAAAAATTAAATGTTATCGAAGCCAAAAATGAAGAA
CACGAAGCGGAACGTATTGTTGGGGAATTAATCGCCCATCGTTTTTCCAACAAAACCAAATACAAAGATTATGCGATTTT
ATATCGTGGTAATCATCAGTCTCGCTTGCTGGAAAAATTGCTGATGCAAAACCGTATCCCCTATAAAATTTCCGGCGGAA
CGTCATTTTTCGCCCGTGCCGAAATTAAAGATATGATGGCTTACTTGCGTTTAGTAGTGAATCAAGACGATGATGCGGCG
TTTTTACGTATCGTTAATACCCCAAAACGAGAAATCGGTGCGGTAACGCTGGAAAAACTGGGCTTACTTGCCAATGAGAA
ACAGGTCAGCTTGTTTGAAGCGATTTTCGATTTTGAATTGATTCAACGCCTGACACCCAAACCCTATCAAGCACTACAGG
ATTTTGCTCGTTGGATTGTCGAAATTGCCGATCAAGCGGAACGTTCTGATCCAATTGAAGCGGTAAAAGATTTATTGGCA
AAAATTCAATACGAAGCCTATTTGTATGAAACGGCTACTTCGCCGAAAGCGGCAGAAATGCAAAGCCGTAACGTGCAAAC
ATTATTCGAATGGGTGCAAGGTATGTTGGAAGGCGATGAAATCGAAGAACCGATGACGTTAGTACAAGTGGTAAACCGCC
TGACGTTGCGTGATATGTTAGAACGTGGTGAAGACGATGATGAAGCGGACCAAGTGCAATTAATGACGCTGCACGCTTCA
AAAGGTTTGGAGTTTCCGCACGTTTTCTTAGTCGGGATGGAAGAAGGTATTCTGCCGCACCAAACCAGTCTGGATGAAGA
TAATGTCGAGGAAGAACGCCGTCTTGCTTACGTAGGCATTACTCGAGCGCAACAAACGCTAACCTTTTCACTCTGTAAAG
AACGCCGCCAGTACGGTGAAATTATCAAACCGGAACCAAGCCGCTTCTTACTTGAATTACCACAAGACGATTTAGTCTGG
GAAAGTGAAAAACCGAAAATGACCGAAGAACAGAAAGTCGAAAAAACTTCAGCCAATATCGCCAACCTAATGGCAATGCT
CAAGGCGAATAAAAAATAA

Upstream 100 bases:

>100_bases
GATCGCAAAATTTTAAAGAAATTCGACCGCTTGTTGTCTTTCGGCTAAAATATTGCGGTTTAAAAAGTGACTGAAGATAA
AGTAAATAAAAGTAGATAAA

Downstream 100 bases:

>100_bases
GATTTTCCTCACATTTTTATATAGGGGCTAACCGCGTTAGCCCTTATTAATAGTGAATTATTCGAGCGAATACTATTCGC
CCCTACCTAAACAAAACCGC

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MKLNDQQQQAVEYVTGACLVLAGAGSGKTRVIINKIAHLIAHCGYSPKQIAAVTFTNKAAREMRERVAHSIGKENSKGLT
ISTFHTLGFEILKREYKLLGFKSGMTLFDEHDQLALLKHLLPENAAEDKDLLKALISTISNWKNDLLSPEMVLTRVRDER
ERVFSHFYQLYQNQLKAYNALDFDDLIMLPVLLFRQFPEAKTRWQQKVRYLLVDEYQDTNTSQYELIKLLVGNEANFTVV
GDDDQSIYSWRGAKPENMQRLREDFDLKVIKLEQNYRSSRRILHCANILIDNNDHIFQKRLFSNLGEGEKLNVIEAKNEE
HEAERIVGELIAHRFSNKTKYKDYAILYRGNHQSRLLEKLLMQNRIPYKISGGTSFFARAEIKDMMAYLRLVVNQDDDAA
FLRIVNTPKREIGAVTLEKLGLLANEKQVSLFEAIFDFELIQRLTPKPYQALQDFARWIVEIADQAERSDPIEAVKDLLA
KIQYEAYLYETATSPKAAEMQSRNVQTLFEWVQGMLEGDEIEEPMTLVQVVNRLTLRDMLERGEDDDEADQVQLMTLHAS
KGLEFPHVFLVGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQYGEIIKPEPSRFLLELPQDDLVW
ESEKPKMTEEQKVEKTSANIANLMAMLKANKK

Sequences:

>Translated_672_residues
MKLNDQQQQAVEYVTGACLVLAGAGSGKTRVIINKIAHLIAHCGYSPKQIAAVTFTNKAAREMRERVAHSIGKENSKGLT
ISTFHTLGFEILKREYKLLGFKSGMTLFDEHDQLALLKHLLPENAAEDKDLLKALISTISNWKNDLLSPEMVLTRVRDER
ERVFSHFYQLYQNQLKAYNALDFDDLIMLPVLLFRQFPEAKTRWQQKVRYLLVDEYQDTNTSQYELIKLLVGNEANFTVV
GDDDQSIYSWRGAKPENMQRLREDFDLKVIKLEQNYRSSRRILHCANILIDNNDHIFQKRLFSNLGEGEKLNVIEAKNEE
HEAERIVGELIAHRFSNKTKYKDYAILYRGNHQSRLLEKLLMQNRIPYKISGGTSFFARAEIKDMMAYLRLVVNQDDDAA
FLRIVNTPKREIGAVTLEKLGLLANEKQVSLFEAIFDFELIQRLTPKPYQALQDFARWIVEIADQAERSDPIEAVKDLLA
KIQYEAYLYETATSPKAAEMQSRNVQTLFEWVQGMLEGDEIEEPMTLVQVVNRLTLRDMLERGEDDDEADQVQLMTLHAS
KGLEFPHVFLVGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQYGEIIKPEPSRFLLELPQDDLVW
ESEKPKMTEEQKVEKTSANIANLMAMLKANKK
>Mature_672_residues
MKLNDQQQQAVEYVTGACLVLAGAGSGKTRVIINKIAHLIAHCGYSPKQIAAVTFTNKAAREMRERVAHSIGKENSKGLT
ISTFHTLGFEILKREYKLLGFKSGMTLFDEHDQLALLKHLLPENAAEDKDLLKALISTISNWKNDLLSPEMVLTRVRDER
ERVFSHFYQLYQNQLKAYNALDFDDLIMLPVLLFRQFPEAKTRWQQKVRYLLVDEYQDTNTSQYELIKLLVGNEANFTVV
GDDDQSIYSWRGAKPENMQRLREDFDLKVIKLEQNYRSSRRILHCANILIDNNDHIFQKRLFSNLGEGEKLNVIEAKNEE
HEAERIVGELIAHRFSNKTKYKDYAILYRGNHQSRLLEKLLMQNRIPYKISGGTSFFARAEIKDMMAYLRLVVNQDDDAA
FLRIVNTPKREIGAVTLEKLGLLANEKQVSLFEAIFDFELIQRLTPKPYQALQDFARWIVEIADQAERSDPIEAVKDLLA
KIQYEAYLYETATSPKAAEMQSRNVQTLFEWVQGMLEGDEIEEPMTLVQVVNRLTLRDMLERGEDDDEADQVQLMTLHAS
KGLEFPHVFLVGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQYGEIIKPEPSRFLLELPQDDLVW
ESEKPKMTEEQKVEKTSANIANLMAMLKANKK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=672, Percent_Identity=66.6666666666667, Blast_Score=923, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=638, Percent_Identity=40.1253918495298, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI1787196, Length=351, Percent_Identity=29.0598290598291, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=792, Percent_Identity=28.2828282828283, Blast_Score=212, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77591; Mature: 77591

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNDQQQQAVEYVTGACLVLAGAGSGKTRVIINKIAHLIAHCGYSPKQIAAVTFTNKAA
CCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECCHHH
REMRERVAHSIGKENSKGLTISTFHTLGFEILKREYKLLGFKSGMTLFDEHDQLALLKHL
HHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHEECCCCCHHCCCHHHHHHHHH
LPENAAEDKDLLKALISTISNWKNDLLSPEMVLTRVRDERERVFSHFYQLYQNQLKAYNA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDFDDLIMLPVLLFRQFPEAKTRWQQKVRYLLVDEYQDTNTSQYELIKLLVGNEANFTVV
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEE
GDDDQSIYSWRGAKPENMQRLREDFDLKVIKLEQNYRSSRRILHCANILIDNNDHIFQKR
CCCCCHHHHCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHEECCCCHHHHHH
LFSNLGEGEKLNVIEAKNEEHEAERIVGELIAHRFSNKTKYKDYAILYRGNHQSRLLEKL
HHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHH
LMQNRIPYKISGGTSFFARAEIKDMMAYLRLVVNQDDDAAFLRIVNTPKREIGAVTLEKL
HHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHH
GLLANEKQVSLFEAIFDFELIQRLTPKPYQALQDFARWIVEIADQAERSDPIEAVKDLLA
HHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KIQYEAYLYETATSPKAAEMQSRNVQTLFEWVQGMLEGDEIEEPMTLVQVVNRLTLRDML
HHHHHHHEEECCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
ERGEDDDEADQVQLMTLHASKGLEFPHVFLVGMEEGILPHQTSLDEDNVEEERRLAYVGI
HCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHEEH
TRAQQTLTFSLCKERRQYGEIIKPEPSRFLLELPQDDLVWESEKPKMTEEQKVEKTSANI
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCCEECCCCCCCCHHHHHHHHHHHH
ANLMAMLKANKK
HHHHHHHHCCCC
>Mature Secondary Structure
MKLNDQQQQAVEYVTGACLVLAGAGSGKTRVIINKIAHLIAHCGYSPKQIAAVTFTNKAA
CCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECCHHH
REMRERVAHSIGKENSKGLTISTFHTLGFEILKREYKLLGFKSGMTLFDEHDQLALLKHL
HHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHEECCCCCHHCCCHHHHHHHHH
LPENAAEDKDLLKALISTISNWKNDLLSPEMVLTRVRDERERVFSHFYQLYQNQLKAYNA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDFDDLIMLPVLLFRQFPEAKTRWQQKVRYLLVDEYQDTNTSQYELIKLLVGNEANFTVV
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEE
GDDDQSIYSWRGAKPENMQRLREDFDLKVIKLEQNYRSSRRILHCANILIDNNDHIFQKR
CCCCCHHHHCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHEECCCCHHHHHH
LFSNLGEGEKLNVIEAKNEEHEAERIVGELIAHRFSNKTKYKDYAILYRGNHQSRLLEKL
HHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHH
LMQNRIPYKISGGTSFFARAEIKDMMAYLRLVVNQDDDAAFLRIVNTPKREIGAVTLEKL
HHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHH
GLLANEKQVSLFEAIFDFELIQRLTPKPYQALQDFARWIVEIADQAERSDPIEAVKDLLA
HHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KIQYEAYLYETATSPKAAEMQSRNVQTLFEWVQGMLEGDEIEEPMTLVQVVNRLTLRDML
HHHHHHHEEECCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
ERGEDDDEADQVQLMTLHASKGLEFPHVFLVGMEEGILPHQTSLDEDNVEEERRLAYVGI
HCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHEEH
TRAQQTLTFSLCKERRQYGEIIKPEPSRFLLELPQDDLVWESEKPKMTEEQKVEKTSANI
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCCEECCCCCCCCHHHHHHHHHHHH
ANLMAMLKANKK
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]