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The map label for this gene is thrA [H]

Identifier: 126207738

GI number: 126207738

Start: 271317

End: 273773

Strand: Reverse

Name: thrA [H]

Synonym: APL_0250

Alternate gene names: 126207738

Gene position: 273773-271317 (Counterclockwise)

Preceding gene: 126207740

Following gene: 126207737

Centisome position: 12.04

GC content: 43.87

Gene sequence:

>2457_bases
ATGCGAGTTCTTAAATTTGGCGGTACATCCCTTGCAAACCCGGAGCGTTTTATCCAAGCGGCGGATATTATTGAAAAGGC
GCATTTGAAAGATCAAGCCGCAGGCGTACTTTCCGCACCGGCAAAAATCACCAACCACCTTGTTGCAATTGTCGATAAAG
CGATTGCAGGCGAATCATTCGAATCCAACCTAAAAGAAGCCACCGAAATTTTCCACAATATCATCAACGGATTATACGCA
ATCAATAATAAGGTTGATCGTGAAGGCTTATTAGCTTTAGTAGAAGCGGAACTCGCACAAATTCAAAGTGTGGCAAGTGA
AGCGGCAAAAAGCAAATCATTACCGGATAGTGTTGCGGCGACGATTCACTGCCGTGGCGAAAAACTATCGATTGCAATGA
TGCAAGCGTGGTTTGAAGCAAAAGGTTATGAAGTTACTCGTATTGATCCTGTCGAAAAATTATTAGCGCACGGCAGTTAT
TTAGAATCCTCCGTCGATATTGCCGAATCAACCAAACGTGTCGATGCTAATTCGATTCCAAAGAAAAATGTCGTATTAAT
GGCAGGTTTTACCGCTGGTAATGAAAACGGTGAACTGGTCTTACTCGGTCGTAACGGTTCCGACTATTCGGCAGCATGTT
TAGCCGCTTGTTTAAAAGCGGATTGTTGCGAAATTTGGACGGATGTGGACGGTGTTTATACTTGTGACCCTCGCTTAGTG
CCTGAAGCGATTTGTTTAGAATCAATGAGTTATCAAGAAGCGATGGAGCTTTCTTACTTCGGTGCGAAAGTCATTCACCC
TCGTACTATCGGTCCGTTAGTGCCGTTAAATATTCCATGTTTAATTAAAAATACCGCAAATCCTGATGCGAAAGGCACAT
TAATCGACGGCAACGTAGCGACTGACGGTTTAAAAGTAAAAGGCATTACTAATCTTGATAATGTTGCAATGTTTAACGTA
TCCGGTGCCGGTATGCAAGGTATGGTCGGTATGGCGGCTCGCGTATTCTCGACTATGTCGAAAGCCGGCATTTCGGTCAT
TCTAATTACTCAATCTTCTTCCGAATACAGTATCAGTTTCTGCGTACCTGCAAAATTTGAGGAAAAAGCGACCGCTTGCT
TAAATGCGGAATTTGCTCAAGAGTTAAGTAAAGGCGATTTGGATCCGATTGATATGATCCGTGAGCTTTCTATCATCTCG
GTAGTCGGTGACGGTATGCGTACCGCAAAGGGAATTGCCGCTCGTTTCTTCTCTTCACTGGCACAAGCCAATATCAGCAT
CGTTGCCATTGCACAAGGTTCGTCCGAGCGTTCAATTTCAGCGGTAGTGCCGATGAATAAAGCGATTGAAGCGGTTAAAG
CGACCCATCAAGGTTTATTCAGCAATAAAAAATCGATTGAAGTCTTTCTAGTCGGTGTAGGCGGAGTCGGAAGCGAACTT
ATCGATCAAATCAAACAACAAAAAGAATTTTTAGCAAAGAAGGATATTGAAATCCGTGTATGCGCATTGGCGAATGCGGA
TAAAATGCTCCTCAACGAAAACGGTTTAAATTTAGATAATTGGAAAAGCGATTTAGAAACGGCAACCCAACCGTCCGATT
TCGACGTTTTACTCTCGTTTATTAAGTTACACCACGTAGTAAATCCGGTATTTGTCGATTGTACCGCGGCGCAATCGGTA
GCAAACCTTTATACCCGAGCGTTAAAAGAAGGTTTCCACGTTGTTACCCCAAACAAGAAAGCCAATACCGGTAGTTACCG
ATATTACCAAGAATTACGTGAAGCTGCTCGCCAAGGTCAGCATAAATTCTTATACGAAACGAACGTAGGCGCAGGCTTAC
CGGTTATTGAAAACTTACAAAACTTGTTGGCTGCCGGTGACGAAGTGGAAAAATTCGAGGGGATTTTATCCGGCTCGCTT
TCATTTATTTTCGGTAAATTAGACGAAGGTTTAAGCCTTTCGGAAGCAACGTTAATCGCAAAAGAAAAAGGCTTTACCGA
GCCGGATCCGCGTGATGACCTTTCAGGTGCGGATGTCGCTCGTAAACTATTGATTCTTGCGCGCGAAACCGGTTTGGCAC
TGGAATTCGACGATATTGAAGTGGAAGGCGTACTACCGAAGGGCTTCTCGGACGGTATGAGTAAAGAAGAATTCTTAAAA
GTCTTGCCGGAAATCGACGCACAGTTTAATGAACGAGTTCAAGCGGCTAAAGCGGAAGGTAAAGTATTACGCTATGTCGG
TTCGATTACCGGCGATAAATGTCGTGTTGCTATTGAAGCGGTAGATGAAAATCACCCGCTTTATAAAGTAAAAGACGGTG
AAAATGCGCTGGCATTCTTAACCCGTTACTATAGCCCGATTCCGCTCTTATTAAGAGGTTACGGTGCAGGTACGGACGTA
ACTGCCGCCGGTATTTTTGCCGATATTCTGCGTACATTACACGGAGGTTCAAACTAA

Upstream 100 bases:

>100_bases
TACCAGTAAAACGGCAAGAAACAAACGGAACCCGCTAGCAAGCGGGTTTTTTTATATCCAAATTTGCAAATACCAATATC
TAAAATCATCAGGAGAAATT

Downstream 100 bases:

>100_bases
TGACGACATTACGAATTTATGCTCCGGCATCAAGTGCCAATCTAAGTGTCGGTTTTGACTCGTTAGGTGCGGCGATTTCA
CCGATTGACGGCTCGTTACT

Product: bifunctional aspartokinase I/homeserine dehydrogenase I

Products: NA

Alternate protein names: AK-HD; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 818; Mature: 818

Protein sequence:

>818_residues
MRVLKFGGTSLANPERFIQAADIIEKAHLKDQAAGVLSAPAKITNHLVAIVDKAIAGESFESNLKEATEIFHNIINGLYA
INNKVDREGLLALVEAELAQIQSVASEAAKSKSLPDSVAATIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSY
LESSVDIAESTKRVDANSIPKKNVVLMAGFTAGNENGELVLLGRNGSDYSAACLAACLKADCCEIWTDVDGVYTCDPRLV
PEAICLESMSYQEAMELSYFGAKVIHPRTIGPLVPLNIPCLIKNTANPDAKGTLIDGNVATDGLKVKGITNLDNVAMFNV
SGAGMQGMVGMAARVFSTMSKAGISVILITQSSSEYSISFCVPAKFEEKATACLNAEFAQELSKGDLDPIDMIRELSIIS
VVGDGMRTAKGIAARFFSSLAQANISIVAIAQGSSERSISAVVPMNKAIEAVKATHQGLFSNKKSIEVFLVGVGGVGSEL
IDQIKQQKEFLAKKDIEIRVCALANADKMLLNENGLNLDNWKSDLETATQPSDFDVLLSFIKLHHVVNPVFVDCTAAQSV
ANLYTRALKEGFHVVTPNKKANTGSYRYYQELREAARQGQHKFLYETNVGAGLPVIENLQNLLAAGDEVEKFEGILSGSL
SFIFGKLDEGLSLSEATLIAKEKGFTEPDPRDDLSGADVARKLLILARETGLALEFDDIEVEGVLPKGFSDGMSKEEFLK
VLPEIDAQFNERVQAAKAEGKVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFLTRYYSPIPLLLRGYGAGTDV
TAAGIFADILRTLHGGSN

Sequences:

>Translated_818_residues
MRVLKFGGTSLANPERFIQAADIIEKAHLKDQAAGVLSAPAKITNHLVAIVDKAIAGESFESNLKEATEIFHNIINGLYA
INNKVDREGLLALVEAELAQIQSVASEAAKSKSLPDSVAATIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSY
LESSVDIAESTKRVDANSIPKKNVVLMAGFTAGNENGELVLLGRNGSDYSAACLAACLKADCCEIWTDVDGVYTCDPRLV
PEAICLESMSYQEAMELSYFGAKVIHPRTIGPLVPLNIPCLIKNTANPDAKGTLIDGNVATDGLKVKGITNLDNVAMFNV
SGAGMQGMVGMAARVFSTMSKAGISVILITQSSSEYSISFCVPAKFEEKATACLNAEFAQELSKGDLDPIDMIRELSIIS
VVGDGMRTAKGIAARFFSSLAQANISIVAIAQGSSERSISAVVPMNKAIEAVKATHQGLFSNKKSIEVFLVGVGGVGSEL
IDQIKQQKEFLAKKDIEIRVCALANADKMLLNENGLNLDNWKSDLETATQPSDFDVLLSFIKLHHVVNPVFVDCTAAQSV
ANLYTRALKEGFHVVTPNKKANTGSYRYYQELREAARQGQHKFLYETNVGAGLPVIENLQNLLAAGDEVEKFEGILSGSL
SFIFGKLDEGLSLSEATLIAKEKGFTEPDPRDDLSGADVARKLLILARETGLALEFDDIEVEGVLPKGFSDGMSKEEFLK
VLPEIDAQFNERVQAAKAEGKVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFLTRYYSPIPLLLRGYGAGTDV
TAAGIFADILRTLHGGSN
>Mature_818_residues
MRVLKFGGTSLANPERFIQAADIIEKAHLKDQAAGVLSAPAKITNHLVAIVDKAIAGESFESNLKEATEIFHNIINGLYA
INNKVDREGLLALVEAELAQIQSVASEAAKSKSLPDSVAATIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSY
LESSVDIAESTKRVDANSIPKKNVVLMAGFTAGNENGELVLLGRNGSDYSAACLAACLKADCCEIWTDVDGVYTCDPRLV
PEAICLESMSYQEAMELSYFGAKVIHPRTIGPLVPLNIPCLIKNTANPDAKGTLIDGNVATDGLKVKGITNLDNVAMFNV
SGAGMQGMVGMAARVFSTMSKAGISVILITQSSSEYSISFCVPAKFEEKATACLNAEFAQELSKGDLDPIDMIRELSIIS
VVGDGMRTAKGIAARFFSSLAQANISIVAIAQGSSERSISAVVPMNKAIEAVKATHQGLFSNKKSIEVFLVGVGGVGSEL
IDQIKQQKEFLAKKDIEIRVCALANADKMLLNENGLNLDNWKSDLETATQPSDFDVLLSFIKLHHVVNPVFVDCTAAQSV
ANLYTRALKEGFHVVTPNKKANTGSYRYYQELREAARQGQHKFLYETNVGAGLPVIENLQNLLAAGDEVEKFEGILSGSL
SFIFGKLDEGLSLSEATLIAKEKGFTEPDPRDDLSGADVARKLLILARETGLALEFDDIEVEGVLPKGFSDGMSKEEFLK
VLPEIDAQFNERVQAAKAEGKVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFLTRYYSPIPLLLRGYGAGTDV
TAAGIFADILRTLHGGSN

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=814, Percent_Identity=62.1621621621622, Blast_Score=998, Evalue=0.0,
Organism=Escherichia coli, GI1790376, Length=813, Percent_Identity=29.7662976629766, Blast_Score=337, Evalue=2e-93,
Organism=Escherichia coli, GI1790455, Length=463, Percent_Identity=30.4535637149028, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6322599, Length=338, Percent_Identity=34.6153846153846, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6320893, Length=507, Percent_Identity=28.9940828402367, Blast_Score=155, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 88031; Mature: 88031

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLKFGGTSLANPERFIQAADIIEKAHLKDQAAGVLSAPAKITNHLVAIVDKAIAGESF
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCCH
ESNLKEATEIFHNIINGLYAINNKVDREGLLALVEAELAQIQSVASEAAKSKSLPDSVAA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEE
TIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSYLESSVDIAESTKRVDANSIP
EEEECCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
KKNVVLMAGFTAGNENGELVLLGRNGSDYSAACLAACLKADCCEIWTDVDGVYTCDPRLV
CCCEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
PEAICLESMSYQEAMELSYFGAKVIHPRTIGPLVPLNIPCLIKNTANPDAKGTLIDGNVA
HHHHHHHHCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCEEEECCCC
TDGLKVKGITNLDNVAMFNVSGAGMQGMVGMAARVFSTMSKAGISVILITQSSSEYSISF
CCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEE
CVPAKFEEKATACLNAEFAQELSKGDLDPIDMIRELSIISVVGDGMRTAKGIAARFFSSL
EECCCCHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AQANISIVAIAQGSSERSISAVVPMNKAIEAVKATHQGLFSNKKSIEVFLVGVGGVGSEL
HHCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHH
IDQIKQQKEFLAKKDIEIRVCALANADKMLLNENGLNLDNWKSDLETATQPSDFDVLLSF
HHHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHH
IKLHHVVNPVFVDCTAAQSVANLYTRALKEGFHVVTPNKKANTGSYRYYQELREAARQGQ
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCC
HKFLYETNVGAGLPVIENLQNLLAAGDEVEKFEGILSGSLSFIFGKLDEGLSLSEATLIA
CEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHEEE
KEKGFTEPDPRDDLSGADVARKLLILARETGLALEFDDIEVEGVLPKGFSDGMSKEEFLK
HHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEEEECCCCCCCCCCHHHHHH
VLPEIDAQFNERVQAAKAEGKVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFL
HHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCHHHHHH
TRYYSPIPLLLRGYGAGTDVTAAGIFADILRTLHGGSN
HHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRVLKFGGTSLANPERFIQAADIIEKAHLKDQAAGVLSAPAKITNHLVAIVDKAIAGESF
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCCH
ESNLKEATEIFHNIINGLYAINNKVDREGLLALVEAELAQIQSVASEAAKSKSLPDSVAA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEE
TIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSYLESSVDIAESTKRVDANSIP
EEEECCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
KKNVVLMAGFTAGNENGELVLLGRNGSDYSAACLAACLKADCCEIWTDVDGVYTCDPRLV
CCCEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
PEAICLESMSYQEAMELSYFGAKVIHPRTIGPLVPLNIPCLIKNTANPDAKGTLIDGNVA
HHHHHHHHCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCEEEECCCC
TDGLKVKGITNLDNVAMFNVSGAGMQGMVGMAARVFSTMSKAGISVILITQSSSEYSISF
CCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEE
CVPAKFEEKATACLNAEFAQELSKGDLDPIDMIRELSIISVVGDGMRTAKGIAARFFSSL
EECCCCHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AQANISIVAIAQGSSERSISAVVPMNKAIEAVKATHQGLFSNKKSIEVFLVGVGGVGSEL
HHCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHH
IDQIKQQKEFLAKKDIEIRVCALANADKMLLNENGLNLDNWKSDLETATQPSDFDVLLSF
HHHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHH
IKLHHVVNPVFVDCTAAQSVANLYTRALKEGFHVVTPNKKANTGSYRYYQELREAARQGQ
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCC
HKFLYETNVGAGLPVIENLQNLLAAGDEVEKFEGILSGSLSFIFGKLDEGLSLSEATLIA
CEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHEEE
KEKGFTEPDPRDDLSGADVARKLLILARETGLALEFDDIEVEGVLPKGFSDGMSKEEFLK
HHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEEEECCCCCCCCCCHHHHHH
VLPEIDAQFNERVQAAKAEGKVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFL
HHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCHHHHHH
TRYYSPIPLLLRGYGAGTDVTAAGIFADILRTLHGGSN
HHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]