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The map label for this gene is thrS

Identifier: 126207707

GI number: 126207707

Start: 241585

End: 243516

Strand: Direct

Name: thrS

Synonym: APL_0219

Alternate gene names: 126207707

Gene position: 241585-243516 (Clockwise)

Preceding gene: 126207695

Following gene: 126207708

Centisome position: 10.62

GC content: 44.36

Gene sequence:

>1932_bases
ATGCCTATTATCACTTTACCAGACGGCTCACAACGCCAATTCGATAATCCCGTTTCTGTAATGGAAGTTGCGCAATCAAT
CGGTGCAGGCTTAGCAAAGGCTACGATTGCCGGTCGTGTAAACGGTGAGCGCCGTGATGCGTGTGACATCATTTCTGAAG
ATAGCTCACTTGAAATTATTACCGCAAAAGATGAAGACGGTTTAGAGATCATTCGTCACTCATGTGCGCACTTATTAGGT
CACGCGATCAAACAATTATTCCCGAATGTAAAAATGGCGATTGGCCCGACAATCGACAACGGTTTCTATTATGATGTGGA
TTTAGATCGCTCGCTCACACAAGAAGATTTAGATGCGATTGAAAAGCGTATGCTTGAGCTGGCTAAAACCAACTATGACG
TAGTGAAAAAACGTGTGAGCTGGCAAGAAGCGCGTGATACGTTTGAAAAACGTGGCGAGCCGTACAAAATGGCGATTTTA
GATGAAAATATCGAACGTACTGCAACGCCAGCGTTATATCATCACGAAGAATATATCGATATGTGTCGTGGTCCGCACGT
ACCGAATATGCGTTTCTGCCACCACTTCAAATTACAGAAAGTAGCGGGTGCATACTGGCGTGGCGACAGCAAAAACAAAA
TGCTGCAACGTATTTACGGCACGGCTTGGGCGGATAAAAAACAATTAGCGGAATACTTAACCCGTTTAGAAGAAGCGGCA
AAACGTGACCACCGCCGTATCGGTAAAGCGTTAGACTTATACCATATGCAAGAAGAAGCGCCGGGTTTGGTGTTCTGGCA
CAATGACGGTTGGACGATTTTCCGTGAACTTGAGACTTTCGTACGTACTAAATTAAAAGAGTACGATTACCAAGAAGTAA
AAGGTCCGTTTATGATGGACAGAGTGCTTTGGGAACGTACCGGTCACTGGCAAAACTATGCGGATTTAATGTTCACGACT
CAATCTGAAAACCGTGAATATGCAATCAAACCGATGAACTGCCCGGGTCACGTTCAAATCTTCAATCAAGGTTTAAAATC
ATACCGTGACTTACCAATCCGTATGGCAGAATTCGGTTCATGCCACCGTAACGAGCCGTCTGGTTCATTACACGGCTTAA
TGCGTGTACGTGGTTTTACCCAAGATGATGCGCATATTTTCTGTACCGAAGATCAAATTGAAAGCGAAGTAACTAGCTGT
ATCCGTATGGTTTACGATATCTACAGCACTTTTGGTTTCAGCAACATTCAAGTGAAACTTTCAACTCGTCCAGAAAACCG
TATCGGCGATGATGCGATGTGGGATCGTGCGGAAGACGGCTTAGCAAAAGCATTAACGGCAAACGGCTTAAGCTATGAAA
TCCAAGAAGGCGAAGGCGCATTCTACGGTCCGAAAATTGAGTTTGCATTACGTGACTGTTTAGATCGTGAATGGCAATGC
GGTACGATTCAATTAGACTTCGCATTACCGGGTCGCTTAGATGCGTCTTATGTAGCGGAAGATAACGGTCGCCGTACACC
GGTTATGATTCACCGTGCGATTTTAGGTTCTATCGAACGCTTTATCGGTATCATCACCGAAGAATACGCAGGTTTCTTCC
CAACGTGGTTAGCACCGACTCAAGCAGTGGTAATGAATATTACCGATAGCCAAGCAGACTACGTACAGAAAGTAACGAAA
GCGTTATCTGATGCGGGTATCCGTGTGAAATCAGACTTACGTAACGAAAAAGTCGGCTTTAAAGTACGTGAACACACCTT
ACGTCGAGTACCTTATATGCTTGTGTGTGGCGATAAAGAAATCGAAGCAGGCAAAGTATCGGTGCGTACCCGTAAAGGTG
CGGATTTAGGCACATTCACCATTGATGAATTTGTTGAAATCCTTAAAAACCAAGTGAAAGCTCGTGGACTTAAATTATTA
GGCGAAGAGTAA

Upstream 100 bases:

>100_bases
TGTAAAAAAAGATTCAAATCAGACCGCTTGTCAGCGGTTTTTATTTTTAAACCAACAAGTCACCTCTCGTATGGGTGACC
ACTGTAACAAGGATATAAAA

Downstream 100 bases:

>100_bases
TTTCATTATTTGTAAAAAAGCGGTCAAATTTGACCGCTTTTTTGTTTATGTATAAGTAAAGTCAATTTTGTATGTTTTAA
AAATATTCTTTACATGTTAT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MPIITLPDGSQRQFDNPVSVMEVAQSIGAGLAKATIAGRVNGERRDACDIISEDSSLEIITAKDEDGLEIIRHSCAHLLG
HAIKQLFPNVKMAIGPTIDNGFYYDVDLDRSLTQEDLDAIEKRMLELAKTNYDVVKKRVSWQEARDTFEKRGEPYKMAIL
DENIERTATPALYHHEEYIDMCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAA
KRDHRRIGKALDLYHMQEEAPGLVFWHNDGWTIFRELETFVRTKLKEYDYQEVKGPFMMDRVLWERTGHWQNYADLMFTT
QSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSC
IRMVYDIYSTFGFSNIQVKLSTRPENRIGDDAMWDRAEDGLAKALTANGLSYEIQEGEGAFYGPKIEFALRDCLDREWQC
GTIQLDFALPGRLDASYVAEDNGRRTPVMIHRAILGSIERFIGIITEEYAGFFPTWLAPTQAVVMNITDSQADYVQKVTK
ALSDAGIRVKSDLRNEKVGFKVREHTLRRVPYMLVCGDKEIEAGKVSVRTRKGADLGTFTIDEFVEILKNQVKARGLKLL
GEE

Sequences:

>Translated_643_residues
MPIITLPDGSQRQFDNPVSVMEVAQSIGAGLAKATIAGRVNGERRDACDIISEDSSLEIITAKDEDGLEIIRHSCAHLLG
HAIKQLFPNVKMAIGPTIDNGFYYDVDLDRSLTQEDLDAIEKRMLELAKTNYDVVKKRVSWQEARDTFEKRGEPYKMAIL
DENIERTATPALYHHEEYIDMCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAA
KRDHRRIGKALDLYHMQEEAPGLVFWHNDGWTIFRELETFVRTKLKEYDYQEVKGPFMMDRVLWERTGHWQNYADLMFTT
QSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSC
IRMVYDIYSTFGFSNIQVKLSTRPENRIGDDAMWDRAEDGLAKALTANGLSYEIQEGEGAFYGPKIEFALRDCLDREWQC
GTIQLDFALPGRLDASYVAEDNGRRTPVMIHRAILGSIERFIGIITEEYAGFFPTWLAPTQAVVMNITDSQADYVQKVTK
ALSDAGIRVKSDLRNEKVGFKVREHTLRRVPYMLVCGDKEIEAGKVSVRTRKGADLGTFTIDEFVEILKNQVKARGLKLL
GEE
>Mature_642_residues
PIITLPDGSQRQFDNPVSVMEVAQSIGAGLAKATIAGRVNGERRDACDIISEDSSLEIITAKDEDGLEIIRHSCAHLLGH
AIKQLFPNVKMAIGPTIDNGFYYDVDLDRSLTQEDLDAIEKRMLELAKTNYDVVKKRVSWQEARDTFEKRGEPYKMAILD
ENIERTATPALYHHEEYIDMCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAAK
RDHRRIGKALDLYHMQEEAPGLVFWHNDGWTIFRELETFVRTKLKEYDYQEVKGPFMMDRVLWERTGHWQNYADLMFTTQ
SENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSCI
RMVYDIYSTFGFSNIQVKLSTRPENRIGDDAMWDRAEDGLAKALTANGLSYEIQEGEGAFYGPKIEFALRDCLDREWQCG
TIQLDFALPGRLDASYVAEDNGRRTPVMIHRAILGSIERFIGIITEEYAGFFPTWLAPTQAVVMNITDSQADYVQKVTKA
LSDAGIRVKSDLRNEKVGFKVREHTLRRVPYMLVCGDKEIEAGKVSVRTRKGADLGTFTIDEFVEILKNQVKARGLKLLG
EE

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=619, Percent_Identity=40.2261712439418, Blast_Score=467, Evalue=1e-131,
Organism=Homo sapiens, GI38202255, Length=633, Percent_Identity=39.3364928909953, Blast_Score=464, Evalue=1e-130,
Organism=Homo sapiens, GI20070344, Length=629, Percent_Identity=35.6120826709062, Blast_Score=396, Evalue=1e-110,
Organism=Escherichia coli, GI1788013, Length=642, Percent_Identity=77.8816199376947, Blast_Score=1088, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984192, Length=629, Percent_Identity=38.6327503974563, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71984184, Length=629, Percent_Identity=38.6327503974563, Blast_Score=461, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322112, Length=650, Percent_Identity=36.4615384615385, Blast_Score=433, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322654, Length=419, Percent_Identity=37.4701670644391, Blast_Score=303, Evalue=5e-83,
Organism=Drosophila melanogaster, GI24583839, Length=632, Percent_Identity=39.5569620253165, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24583842, Length=632, Percent_Identity=39.5569620253165, Blast_Score=480, Evalue=1e-135,
Organism=Drosophila melanogaster, GI28574141, Length=632, Percent_Identity=39.5569620253165, Blast_Score=480, Evalue=1e-135,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_ACTP2 (A3MYU1)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052932.1
- ProteinModelPortal:   A3MYU1
- SMR:   A3MYU1
- STRING:   A3MYU1
- GeneID:   4850216
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0219
- eggNOG:   COG0441
- HOGENOM:   HBG352811
- OMA:   FHPKGMV
- PhylomeDB:   A3MYU1
- ProtClustDB:   PRK00413
- BioCyc:   APLE416269:APL_0219-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 73561; Mature: 73429

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIITLPDGSQRQFDNPVSVMEVAQSIGAGLAKATIAGRVNGERRDACDIISEDSSLEII
CCEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHCCCCCEEEE
TAKDEDGLEIIRHSCAHLLGHAIKQLFPNVKMAIGPTIDNGFYYDVDLDRSLTQEDLDAI
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCEEEEEECCCCCCHHHHHHH
EKRMLELAKTNYDVVKKRVSWQEARDTFEKRGEPYKMAILDENIERTATPALYHHEEYID
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCHHHHHHHHHH
MCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAA
HHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KRDHRRIGKALDLYHMQEEAPGLVFWHNDGWTIFRELETFVRTKLKEYDYQEVKGPFMMD
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHH
RVLWERTGHWQNYADLMFTTQSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGS
HHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEHHHHCC
CHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSCIRMVYDIYSTFGFSNIQVKL
CCCCCCCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
STRPENRIGDDAMWDRAEDGLAKALTANGLSYEIQEGEGAFYGPKIEFALRDCLDREWQC
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCCHHHHHHHHCCCCCCC
GTIQLDFALPGRLDASYVAEDNGRRTPVMIHRAILGSIERFIGIITEEYAGFFPTWLAPT
CEEEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCC
QAVVMNITDSQADYVQKVTKALSDAGIRVKSDLRNEKVGFKVREHTLRRVPYMLVCGDKE
EEEEEECCCCHHHHHHHHHHHHHHCCCEEHHHHCCCCCCCHHHHHHHHHCCEEEEECCCC
IEAGKVSVRTRKGADLGTFTIDEFVEILKNQVKARGLKLLGEE
CCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCHHHCCCC
>Mature Secondary Structure 
PIITLPDGSQRQFDNPVSVMEVAQSIGAGLAKATIAGRVNGERRDACDIISEDSSLEII
CEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHCCCCCEEEE
TAKDEDGLEIIRHSCAHLLGHAIKQLFPNVKMAIGPTIDNGFYYDVDLDRSLTQEDLDAI
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCEEEEEECCCCCCHHHHHHH
EKRMLELAKTNYDVVKKRVSWQEARDTFEKRGEPYKMAILDENIERTATPALYHHEEYID
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCHHHHHHHHHH
MCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAA
HHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KRDHRRIGKALDLYHMQEEAPGLVFWHNDGWTIFRELETFVRTKLKEYDYQEVKGPFMMD
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHH
RVLWERTGHWQNYADLMFTTQSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGS
HHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEHHHHCC
CHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSCIRMVYDIYSTFGFSNIQVKL
CCCCCCCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
STRPENRIGDDAMWDRAEDGLAKALTANGLSYEIQEGEGAFYGPKIEFALRDCLDREWQC
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCCHHHHHHHHCCCCCCC
GTIQLDFALPGRLDASYVAEDNGRRTPVMIHRAILGSIERFIGIITEEYAGFFPTWLAPT
CEEEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCC
QAVVMNITDSQADYVQKVTKALSDAGIRVKSDLRNEKVGFKVREHTLRRVPYMLVCGDKE
EEEEEECCCCHHHHHHHHHHHHHHCCCEEHHHHCCCCCCCHHHHHHHHHCCEEEEECCCC
IEAGKVSVRTRKGADLGTFTIDEFVEILKNQVKARGLKLLGEE
CCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA