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The map label for this gene is nfuA

Identifier: 126207637

GI number: 126207637

Start: 167032

End: 167631

Strand: Reverse

Name: nfuA

Synonym: APL_0149

Alternate gene names: 126207637

Gene position: 167631-167032 (Counterclockwise)

Preceding gene: 126207647

Following gene: 126207634

Centisome position: 7.37

GC content: 44.0

Gene sequence:

>600_bases
ATGGAACAACAAGAAACTATTCATATTTCAATTTCGGAAGCGGCGCAAACACATTTTCGCCGTCTTTTAGAGCAACAGGA
AGAAAATACCAACATTCGTATCTTTGTAGTAAATCCGGGTACGCCTAATGCCGAATGCGGCGTATCTTATTGCCCGCCGA
ATGCGGTTGAAGAAACCGATACTCAGTTCGAATACAACGGTTTCTCCGCTTTTGTCGATGAAATCAGCTTACCGTTTTTA
GATGAAGCCGAAATTGATTATGTAACGGATCCGATGGGATCGCAGCTTACCTTAAAAGCGCCGAATGCGAAAATGCGTAA
AGTTGCCGATGACGCCCCTTTCATCGAACGTTTGGATTATGTCATTCAAACTCAAGTAAATCCGCAATTGGCAAGCCACG
GCGGTCGAGTGACATTAATCGAAGTAACCGAAGATAAATATGCGATTCTGCAATTCGGCGGCGGCTGTAACGGCTGTTCA
ATGGTGGACGTTACCTTAAAAGAAGGGATTGAGAAACAATTGTTAGCAATGTTCCCTGATGAATTGGCGGGCGTAAAAGA
TGTAACCGAACATCAACGCGGCGAACATTCTTACTACTAA

Upstream 100 bases:

>100_bases
AATTCGGATTTGTAAAATTTTGAGTAGAAAAAATCGCCTGTAAGGCGTAGAATATCCGATAAATTTAGTCAATTAATTTA
TTACACTTGGAACAAAACTA

Downstream 100 bases:

>100_bases
GCCTAGATAAGTTATAAATGGAAAGCACGAGTAATTTACTCGTGCTTTTTTGTAATCGGGCAAACCGACAGGTTACCAAA
TGCTAAAGACAGATATAAAA

Product: putative DNA uptake protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MEQQETIHISISEAAQTHFRRLLEQQEENTNIRIFVVNPGTPNAECGVSYCPPNAVEETDTQFEYNGFSAFVDEISLPFL
DEAEIDYVTDPMGSQLTLKAPNAKMRKVADDAPFIERLDYVIQTQVNPQLASHGGRVTLIEVTEDKYAILQFGGGCNGCS
MVDVTLKEGIEKQLLAMFPDELAGVKDVTEHQRGEHSYY

Sequences:

>Translated_199_residues
MEQQETIHISISEAAQTHFRRLLEQQEENTNIRIFVVNPGTPNAECGVSYCPPNAVEETDTQFEYNGFSAFVDEISLPFL
DEAEIDYVTDPMGSQLTLKAPNAKMRKVADDAPFIERLDYVIQTQVNPQLASHGGRVTLIEVTEDKYAILQFGGGCNGCS
MVDVTLKEGIEKQLLAMFPDELAGVKDVTEHQRGEHSYY
>Mature_199_residues
MEQQETIHISISEAAQTHFRRLLEQQEENTNIRIFVVNPGTPNAECGVSYCPPNAVEETDTQFEYNGFSAFVDEISLPFL
DEAEIDYVTDPMGSQLTLKAPNAKMRKVADDAPFIERLDYVIQTQVNPQLASHGGRVTLIEVTEDKYAILQFGGGCNGCS
MVDVTLKEGIEKQLLAMFPDELAGVKDVTEHQRGEHSYY

Specific function: Involved in iron-sulfur cluster biogenesis. Binds a 4Fe- 4S cluster, can transfer this cluster to apoproteins, and thereby intervenes in the maturation of Fe/S proteins. Could also act as a scaffold/chaperone for damaged Fe/S proteins

COG id: COG0694

COG function: function code O; Thioredoxin-like proteins and domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nfuA family

Homologues:

Organism=Escherichia coli, GI1789819, Length=191, Percent_Identity=74.3455497382199, Blast_Score=311, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFUA_ACTP2 (A3MYM1)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052862.1
- ProteinModelPortal:   A3MYM1
- STRING:   A3MYM1
- GeneID:   4850137
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0149
- eggNOG:   COG0694
- HOGENOM:   HBG289644
- OMA:   CQGCSAV
- PhylomeDB:   A3MYM1
- ProtClustDB:   PRK11190
- BioCyc:   APLE416269:APL_0149-MONOMER
- HAMAP:   MF_01637
- InterPro:   IPR017726
- InterPro:   IPR000361
- InterPro:   IPR001075
- Gene3D:   G3DSA:2.60.300.12
- PANTHER:   PTHR10072
- ProDom:   PD002830
- TIGRFAMs:   TIGR03341

Pfam domain/function: PF01521 Fe-S_biosyn; PF01106 NifU; SSF89360 HesB_yadR_yfhF-like

EC number: NA

Molecular weight: Translated: 22250; Mature: 22250

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQQETIHISISEAAQTHFRRLLEQQEENTNIRIFVVNPGTPNAECGVSYCPPNAVEETD
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCC
TQFEYNGFSAFVDEISLPFLDEAEIDYVTDPMGSQLTLKAPNAKMRKVADDAPFIERLDY
CEEEECCHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHCCCCHHHHHHHH
VIQTQVNPQLASHGGRVTLIEVTEDKYAILQFGGGCNGCSMVDVTLKEGIEKQLLAMFPD
HHHCCCCHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEHHHCCHHHHHHHHCCH
ELAGVKDVTEHQRGEHSYY
HHCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEQQETIHISISEAAQTHFRRLLEQQEENTNIRIFVVNPGTPNAECGVSYCPPNAVEETD
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCC
TQFEYNGFSAFVDEISLPFLDEAEIDYVTDPMGSQLTLKAPNAKMRKVADDAPFIERLDY
CEEEECCHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHCCCCHHHHHHHH
VIQTQVNPQLASHGGRVTLIEVTEDKYAILQFGGGCNGCSMVDVTLKEGIEKQLLAMFPD
HHHCCCCHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEHHHCCHHHHHHHHCCH
ELAGVKDVTEHQRGEHSYY
HHCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA