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The map label for this gene is dusA [H]

Identifier: 126207634

GI number: 126207634

Start: 162938

End: 163915

Strand: Reverse

Name: dusA [H]

Synonym: APL_0146

Alternate gene names: 126207634

Gene position: 163915-162938 (Counterclockwise)

Preceding gene: 126207637

Following gene: 126207633

Centisome position: 7.21

GC content: 44.99

Gene sequence:

>978_bases
ATGCAAAATCAATTTTATCGCGGACGTTTTTCCGTTGCGCCAATGCTGGATTGGACCACTCGTCATTGTCGCTACTTCCA
TCGTCAATTCAGCCGACACGCTTTGCTTTATACCGAAATGATCACCGCACCGGCGATCATTCACGCCAAATACGATCTAC
TTGAATACGATCCGAGTGAAAATCCGGTTGCGTTACAGCTCGGCGGTAGCGATCCGGCACAGCTTGCGCATTGTGCCAAA
TTAGTTGAAGAACGCGGCTATGCCGAAATCAATTTAAACGTCGGTTGCCCTTCCGACCGTGTGCAAAACGGGATGTTCGG
CGCTTGTTTAATGGCAAAAGCCGATTTAGTTGCCGACTGTATCAAAGCGATGCAAGATGCGGTGCAAATTCCAGTGACGG
TTAAACATCGTATCGGTATTGATGACTTGGACAGTTACGAATTTTTATGTGATTTTATTGAGAAAGTACAGCCGTATAGC
AACGATTTCATCGTTCATGCTCGCAAAGCGTGGTTGTCCGGTTTAAGTCCGAAACAAAATCGTGAAATTCCACCGCTTGA
TTATGAGCGTGTCTATCAACTGAAACGTGATTTTCCGCATTTGAATATTTCAATTAACGGCGGGATTAAAACTATTGAAG
AAATCAAACGGCACTTAGCATTTGTCGATGGCGTAATGGTGGGGCGTGAAGCCTATCAGAATCCGTCACTATTAGGTGAA
ATCGATTCGCAAATTTTCGGTGAAAATCGACCGCTTGTCACCGCCCGTGAAGCGGTGGAAAAAATGTTGCCTTATATTGC
AGTGCAAACGCAGAAAGGTGTGTATTTAAATCATATCGTTCGTCATATGTTAGGCGCATTCCAAAACTGTAAAGGCGCTC
GTCAGTGGCGTCGTCATTTAAGCGAAAATGCGACTAAACAAGGCGCCGGCGTTGAAGTGGTGGAACAAGCGCTTAGCTTT
GTACAAGAAAGTAATTAA

Upstream 100 bases:

>100_bases
ATAAAATTTTGAACTCTATACAAGCGGTCAAATTTAGCCCCAAATTTACCAAAAATCGGCTAAATTCCACCGCTTGTTAT
TTTTATTTATGAATAATCTT

Downstream 100 bases:

>100_bases
ATTTGCTATTTCCCCCTTAAATTCAGATAATGTTTTTATTTGGATTTAAGGGAAAACTTATGGCCGTTCATTGCGTCAAT
CCAAAAGGTAGCCTTGACCA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MQNQFYRGRFSVAPMLDWTTRHCRYFHRQFSRHALLYTEMITAPAIIHAKYDLLEYDPSENPVALQLGGSDPAQLAHCAK
LVEERGYAEINLNVGCPSDRVQNGMFGACLMAKADLVADCIKAMQDAVQIPVTVKHRIGIDDLDSYEFLCDFIEKVQPYS
NDFIVHARKAWLSGLSPKQNREIPPLDYERVYQLKRDFPHLNISINGGIKTIEEIKRHLAFVDGVMVGREAYQNPSLLGE
IDSQIFGENRPLVTAREAVEKMLPYIAVQTQKGVYLNHIVRHMLGAFQNCKGARQWRRHLSENATKQGAGVEVVEQALSF
VQESN

Sequences:

>Translated_325_residues
MQNQFYRGRFSVAPMLDWTTRHCRYFHRQFSRHALLYTEMITAPAIIHAKYDLLEYDPSENPVALQLGGSDPAQLAHCAK
LVEERGYAEINLNVGCPSDRVQNGMFGACLMAKADLVADCIKAMQDAVQIPVTVKHRIGIDDLDSYEFLCDFIEKVQPYS
NDFIVHARKAWLSGLSPKQNREIPPLDYERVYQLKRDFPHLNISINGGIKTIEEIKRHLAFVDGVMVGREAYQNPSLLGE
IDSQIFGENRPLVTAREAVEKMLPYIAVQTQKGVYLNHIVRHMLGAFQNCKGARQWRRHLSENATKQGAGVEVVEQALSF
VQESN
>Mature_325_residues
MQNQFYRGRFSVAPMLDWTTRHCRYFHRQFSRHALLYTEMITAPAIIHAKYDLLEYDPSENPVALQLGGSDPAQLAHCAK
LVEERGYAEINLNVGCPSDRVQNGMFGACLMAKADLVADCIKAMQDAVQIPVTVKHRIGIDDLDSYEFLCDFIEKVQPYS
NDFIVHARKAWLSGLSPKQNREIPPLDYERVYQLKRDFPHLNISINGGIKTIEEIKRHLAFVDGVMVGREAYQNPSLLGE
IDSQIFGENRPLVTAREAVEKMLPYIAVQTQKGVYLNHIVRHMLGAFQNCKGARQWRRHLSENATKQGAGVEVVEQALSF
VQESN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=247, Percent_Identity=28.7449392712551, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI145693211, Length=320, Percent_Identity=68.4375, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1788462, Length=230, Percent_Identity=29.1304347826087, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789660, Length=301, Percent_Identity=23.5880398671096, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=237, Percent_Identity=27.0042194092827, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24580595, Length=258, Percent_Identity=25.1937984496124, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI19920448, Length=258, Percent_Identity=25.1937984496124, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36920; Mature: 36920

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNQFYRGRFSVAPMLDWTTRHCRYFHRQFSRHALLYTEMITAPAIIHAKYDLLEYDPSE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCC
NPVALQLGGSDPAQLAHCAKLVEERGYAEINLNVGCPSDRVQNGMFGACLMAKADLVADC
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHH
IKAMQDAVQIPVTVKHRIGIDDLDSYEFLCDFIEKVQPYSNDFIVHARKAWLSGLSPKQN
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCC
REIPPLDYERVYQLKRDFPHLNISINGGIKTIEEIKRHLAFVDGVMVGREAYQNPSLLGE
CCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IDSQIFGENRPLVTAREAVEKMLPYIAVQTQKGVYLNHIVRHMLGAFQNCKGARQWRRHL
HHHHHHCCCCCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
SENATKQGAGVEVVEQALSFVQESN
HHCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQNQFYRGRFSVAPMLDWTTRHCRYFHRQFSRHALLYTEMITAPAIIHAKYDLLEYDPSE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCC
NPVALQLGGSDPAQLAHCAKLVEERGYAEINLNVGCPSDRVQNGMFGACLMAKADLVADC
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHH
IKAMQDAVQIPVTVKHRIGIDDLDSYEFLCDFIEKVQPYSNDFIVHARKAWLSGLSPKQN
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCC
REIPPLDYERVYQLKRDFPHLNISINGGIKTIEEIKRHLAFVDGVMVGREAYQNPSLLGE
CCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IDSQIFGENRPLVTAREAVEKMLPYIAVQTQKGVYLNHIVRHMLGAFQNCKGARQWRRHL
HHHHHHCCCCCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
SENATKQGAGVEVVEQALSFVQESN
HHCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA