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The map label for this gene is bioB

Identifier: 126207605

GI number: 126207605

Start: 133904

End: 134914

Strand: Direct

Name: bioB

Synonym: APL_0117

Alternate gene names: 126207605

Gene position: 133904-134914 (Clockwise)

Preceding gene: 126207604

Following gene: 126207607

Centisome position: 5.89

GC content: 45.6

Gene sequence:

>1011_bases
ATGACAACCTATAATTTGCGTTTAAAAGCAAAAAGTGAACTTACCCCCCACCCTACGGCACAATATTGGCGTAAATGCCA
AGTCGAAGAATTATTCGAAATGCCTTTTATGGAACTTATTTTTAAAGCGGCACAAGTCCATAGAGAGCATTTTGATCCGC
AAGCGATTCAATTATCGACGTTACTTTCGATTAAAACCGGAGGATGTCCGGAAGAATGCGAATATTGTCCTCAGTCCGCT
CGCTATGATACCGGGCTTGAAAAGCAAGTGATGATGGATGTGGAAGAAATTGTCGAGAAAGCCAAAATAGCCAAAGCGAG
AGGCGCGAGCCGTTTTTGTATGGGCGCCGCTTGGCGTGGACCGAAGCCGAAAGACATTAAAGTGGTGTCTGAAATTATCG
GTGCGGTAAAAGCGTTAGGTCTAGAGACTTGCGGTACTTTCGGTTTACTGGAAGACGGGATGGCGGAAGATTTAAAACAA
GCCGGATTAGACTATTACAATCACAATTTGGATACCGATCCGGAACGCTATAATAAAATCGTACATACGCGTAATCATGA
TGATCGGATGGATACGCTCGGTAAAGTGCGTCATGCCGGTTTAAAAGTATGCTGCGGCGGAATTGTCGGGATGAACGAAA
CTCGTCCGGAACGTGCCGGACTTATCGCAAGTTTAGCGAATCTCGATCCGCAACCGGAAAGCGTACCGATTAACCAATTA
ATTAAAGTCGAAGGTACGCCGTTGGAAAATGCCGAGGATCTGGATTGGACCGAATTTGTCAGAACGATTGCGGTTGCGCG
TATTACTATGCCGAAAAGCTATGTGCGTTTATCGGCAGGTCGAACCTCAATGCCGGAAGCAATGCAAACGTTATGTTTTA
TGGCGGGAGCGAATTCGATTTTCTATGGTGAGAAGTTATTGACAACTTGCAATCCGGAAGAAGACTCCGACCGCTTGTTA
ATGGATAAATTGGATTTGTATCCGCTGGCGTATGAGCCTAGCGACGCTTAG

Upstream 100 bases:

>100_bases
AATAACGTCAGTTAATGTATTGTATAAGCGGTCTATTTTTCTCAATAAATTGTAAGATTTTGAGAAAATACGACCGCTTC
GGTTTATTTAAGGAGATGAC

Downstream 100 bases:

>100_bases
TCCGAAGGAATGAAAAAAGCCAGCATTTGCTGGCTTTTAGATTCTTTATATACACGTTTAAAATTAAAGTGTTTTTACGT
TTACCGCTGAAGGACCGCGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTTYNLRLKAKSELTPHPTAQYWRKCQVEELFEMPFMELIFKAAQVHREHFDPQAIQLSTLLSIKTGGCPEECEYCPQSA
RYDTGLEKQVMMDVEEIVEKAKIAKARGASRFCMGAAWRGPKPKDIKVVSEIIGAVKALGLETCGTFGLLEDGMAEDLKQ
AGLDYYNHNLDTDPERYNKIVHTRNHDDRMDTLGKVRHAGLKVCCGGIVGMNETRPERAGLIASLANLDPQPESVPINQL
IKVEGTPLENAEDLDWTEFVRTIAVARITMPKSYVRLSAGRTSMPEAMQTLCFMAGANSIFYGEKLLTTCNPEEDSDRLL
MDKLDLYPLAYEPSDA

Sequences:

>Translated_336_residues
MTTYNLRLKAKSELTPHPTAQYWRKCQVEELFEMPFMELIFKAAQVHREHFDPQAIQLSTLLSIKTGGCPEECEYCPQSA
RYDTGLEKQVMMDVEEIVEKAKIAKARGASRFCMGAAWRGPKPKDIKVVSEIIGAVKALGLETCGTFGLLEDGMAEDLKQ
AGLDYYNHNLDTDPERYNKIVHTRNHDDRMDTLGKVRHAGLKVCCGGIVGMNETRPERAGLIASLANLDPQPESVPINQL
IKVEGTPLENAEDLDWTEFVRTIAVARITMPKSYVRLSAGRTSMPEAMQTLCFMAGANSIFYGEKLLTTCNPEEDSDRLL
MDKLDLYPLAYEPSDA
>Mature_335_residues
TTYNLRLKAKSELTPHPTAQYWRKCQVEELFEMPFMELIFKAAQVHREHFDPQAIQLSTLLSIKTGGCPEECEYCPQSAR
YDTGLEKQVMMDVEEIVEKAKIAKARGASRFCMGAAWRGPKPKDIKVVSEIIGAVKALGLETCGTFGLLEDGMAEDLKQA
GLDYYNHNLDTDPERYNKIVHTRNHDDRMDTLGKVRHAGLKVCCGGIVGMNETRPERAGLIASLANLDPQPESVPINQLI
KVEGTPLENAEDLDWTEFVRTIAVARITMPKSYVRLSAGRTSMPEAMQTLCFMAGANSIFYGEKLLTTCNPEEDSDRLLM
DKLDLYPLAYEPSDA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=306, Percent_Identity=61.1111111111111, Blast_Score=401, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321725, Length=318, Percent_Identity=44.6540880503145, Blast_Score=311, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_ACTP2 (A3MYI9)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052830.1
- ProteinModelPortal:   A3MYI9
- SMR:   A3MYI9
- STRING:   A3MYI9
- GeneID:   4849847
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0117
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   A3MYI9
- ProtClustDB:   CLSK2305620
- BioCyc:   APLE416269:APL_0117-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37602; Mature: 37470

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYNLRLKAKSELTPHPTAQYWRKCQVEELFEMPFMELIFKAAQVHREHFDPQAIQLST
CCEEEEEEEECCCCCCCCCHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHEEHHH
LLSIKTGGCPEECEYCPQSARYDTGLEKQVMMDVEEIVEKAKIAKARGASRFCMGAAWRG
HHEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
PKPKDIKVVSEIIGAVKALGLETCGTFGLLEDGMAEDLKQAGLDYYNHNLDTDPERYNKI
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHH
VHTRNHDDRMDTLGKVRHAGLKVCCGGIVGMNETRPERAGLIASLANLDPQPESVPINQL
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHE
IKVEGTPLENAEDLDWTEFVRTIAVARITMPKSYVRLSAGRTSMPEAMQTLCFMAGANSI
EEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHEECCCCCCHHHHHHHHHHHHCCCCE
FYGEKLLTTCNPEEDSDRLLMDKLDLYPLAYEPSDA
EECHHHHHCCCCCCCCCHHHHHHHCCCEECCCCCCC
>Mature Secondary Structure 
TTYNLRLKAKSELTPHPTAQYWRKCQVEELFEMPFMELIFKAAQVHREHFDPQAIQLST
CEEEEEEEECCCCCCCCCHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHEEHHH
LLSIKTGGCPEECEYCPQSARYDTGLEKQVMMDVEEIVEKAKIAKARGASRFCMGAAWRG
HHEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
PKPKDIKVVSEIIGAVKALGLETCGTFGLLEDGMAEDLKQAGLDYYNHNLDTDPERYNKI
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHH
VHTRNHDDRMDTLGKVRHAGLKVCCGGIVGMNETRPERAGLIASLANLDPQPESVPINQL
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHE
IKVEGTPLENAEDLDWTEFVRTIAVARITMPKSYVRLSAGRTSMPEAMQTLCFMAGANSI
EEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHEECCCCCCHHHHHHHHHHHHCCCCE
FYGEKLLTTCNPEEDSDRLLMDKLDLYPLAYEPSDA
EECHHHHHCCCCCCCCCHHHHHHHCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA