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The map label for this gene is hupA [H]

Identifier: 126207602

GI number: 126207602

Start: 131960

End: 132232

Strand: Direct

Name: hupA [H]

Synonym: APL_0114

Alternate gene names: 126207602

Gene position: 131960-132232 (Clockwise)

Preceding gene: 126207601

Following gene: 126207604

Centisome position: 5.8

GC content: 42.86

Gene sequence:

>273_bases
ATGAACAAAACTGAGTTAATCGATGCAATCGCAGCTGGTGCAGAGTTAAGCAAGAAAGACGCGAAAGCGGCATTAGAAGC
GACTTTAAATGCGATCTCTGAAAGCCTAAAAAATGGCGACACCGTTCAGTTAATCGGCTTCGGTACTTTTAAAGTAAACG
AGCGTAATGCACGTACGGGTCGTAACCCACGTACCGGCGAAGAAATCAAAATCGCAGCATCTAAAGTGCCGGCGTTTGTT
GCAGGTAAAGCATTAAAAGATTTAGTAAAATAA

Upstream 100 bases:

>100_bases
TGCGAAAAGGAAAGTTTCAATTAAGCTAGTAAACGTTTTACGCAACAATTTCGGACGTGTAACGTCATTTAATCTAAAAT
AAATCTTAGAGGATACAATC

Downstream 100 bases:

>100_bases
TTCTTTGCAAAGTTTTTAAAGCCTAGATTCATTCTAGGCTTTTTTGTTTCTCTTATACCGAGAACGGATATTGGATTGCC
GGATGATATTCGTAACCTTC

Product: DNA-binding protein HU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKTELIDAIAAGAELSKKDAKAALEATLNAISESLKNGDTVQLIGFGTFKVNERNARTGRNPRTGEEIKIAASKVPAFV
AGKALKDLVK

Sequences:

>Translated_90_residues
MNKTELIDAIAAGAELSKKDAKAALEATLNAISESLKNGDTVQLIGFGTFKVNERNARTGRNPRTGEEIKIAASKVPAFV
AGKALKDLVK
>Mature_90_residues
MNKTELIDAIAAGAELSKKDAKAALEATLNAISESLKNGDTVQLIGFGTFKVNERNARTGRNPRTGEEIKIAASKVPAFV
AGKALKDLVK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1790433, Length=90, Percent_Identity=76.6666666666667, Blast_Score=137, Evalue=1e-34,
Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=66.2921348314607, Blast_Score=118, Evalue=9e-29,
Organism=Escherichia coli, GI1788005, Length=90, Percent_Identity=41.1111111111111, Blast_Score=74, Evalue=1e-15,
Organism=Escherichia coli, GI1787141, Length=88, Percent_Identity=35.2272727272727, Blast_Score=69, Evalue=5e-14,

Paralogues:

None

Copy number: 1380 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9497; Mature: 9497

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTELIDAIAAGAELSKKDAKAALEATLNAISESLKNGDTVQLIGFGTFKVNERNARTG
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECEEEECCCCCCCC
RNPRTGEEIKIAASKVPAFVAGKALKDLVK
CCCCCCCEEEEEHHHCCHHHHHHHHHHHHC
>Mature Secondary Structure
MNKTELIDAIAAGAELSKKDAKAALEATLNAISESLKNGDTVQLIGFGTFKVNERNARTG
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECEEEECCCCCCCC
RNPRTGEEIKIAASKVPAFVAGKALKDLVK
CCCCCCCEEEEEHHHCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]