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The map label for this gene is dppA [H]

Identifier: 126207552

GI number: 126207552

Start: 76770

End: 78371

Strand: Direct

Name: dppA [H]

Synonym: APL_0064

Alternate gene names: 126207552

Gene position: 76770-78371 (Clockwise)

Preceding gene: 126207551

Following gene: 126207553

Centisome position: 3.38

GC content: 39.89

Gene sequence:

>1602_bases
ATGAAAAAACTTACAAAACTCTCGCTTCTCTCATTAGCTATAGCGGCTTCAAGTTCCGCTTTAGCTGCCCCAAAAACATT
TGTTTACTGCTTAGAAGCCTCACCGACTTACTTAAATCCGCAATTTGCTACAGACGGCGGTACGCTTGATGCAACCAGCC
AAGCGATTTTTAATCGTTTAGTTGATTTTGCTAAAGGAGAAACAACAGTTATACCTTCTTTAGCGGAAAAATGGGATGTG
TCGGAAGACGGCAAAAGTTATACCTTTTATTTACGTAAAGGGGTGAAATTCCATTCGACTAAAGATTTTAAACCGAGTCG
TGATTTTAATGCGGACGATGTTTTGTTCTCATTCAATCGTCAATTAGATCCGAATCATCCGTATCATAAAATATCCGGTG
GTAACTATGAATATTTTATCGGTATGGATATGGGCAACATTATTCAAAATGTTGAGAAAGTGGATGATTATACGGTTAAA
ATTAATCTAAAAGTTCCGAATGCACCATTCTTAGCAAATATTGCGATGGATTTTGCTTCGATTTTCTCTGCAGAATATGC
GGATAAATTATTGAAAGCGGGAACGCCTGAAAAATTAGATCAAAATCCTATCGGCACCGGTCCATTCCAATTTGTCGATT
ACCAAAAGGATGCTCAGATTCGTTACAAAGGCTTTGATCAATATTGGGCCGGCAAGCCGGCGATTGATCGCTTAGTCTTT
GCAATTACACCGGATGCTTCGGTGCGTATGGCGAAATTACAAAAAGGCGAATGTCATGCAGCACCTTACCCGAATCCGGC
TGATCTGGAAGCATTGAAAAAAGATCCGAATATCAATTTAATGACCAAACCGGGTTTAAATATCGGTTATTTACATTTCA
ATACGCAGAAAAAACCGTTTGATGATGTGAAAGTGCGTCAAGCGTTAAATCATGCAATTAACAAAGATGCGATTATTCAG
TCGGTTTATCAAGGTTCCGGTGAAAAAGCGAAAAATCCGATTCCACCGACAATGTGGAGTTATAACGATGACGTGAAGGA
TTATGACTATAATCCGGAAAAAGCCAAAGCCCTGTTAAAAGAAGCCGGACTTGAAAAAGGTTTTGATACTGAAGTGTGGG
CAATGCCGGTTTCTCGTCCGTATAATCCGAACGCACGTCGTATGGCGGAGCTTATCCAAGAAGATTGGAAAAAAGTGGGT
GTAAATGCCAAAATCGTGAGTTACGAATGGGGTGAATATCTCAATCGTATGCGTGACGGCGAACATACAACCGGTATGAT
GGGCTGGAACGGTGATAACGGTGATCCGGATAACTTCCTAAATACCTTATTAAGTTGTGCGGCGGTAAAACAAGGTTCTA
ACTATGCAAAATTCTGTCATAAAGATTTTGATAAGCTAATGACCGATGCGGTTCAAACAACTGATAAAGCAAAACGTACC
GAGCTTTATAAACAAGCGCAAGTACTTTTCAAAGAACAAGCACCATGGGTAACCATTGCTCACTCAACCACTTATTTCCC
TGTTCGTAAGGAAGTAAAAGGTTATGTGATCAGCCCATTTAGCTTACATAATTTCTACGGTGTTGATTTAGAGGCAAAAT
AA

Upstream 100 bases:

>100_bases
ACCGCTTTTTACTGAAAAAGTATTGAACTTCATCGAAAAACTTGTATATTCAAAGAAAACATAAAAAATAATAATCCTAA
ACACAACACCGGAGTATTCA

Downstream 100 bases:

>100_bases
GTTCTCTTATTAACCATTGAAAAGCACGGCAGAGAGTTATCGGCTATTTGTAAAAAATTCGCTGAATTTTACCGCTTAAT
GCCGTGTTTTTCATATTTTC

Product: periplasmic dipeptide transport protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKKLTKLSLLSLAIAASSSALAAPKTFVYCLEASPTYLNPQFATDGGTLDATSQAIFNRLVDFAKGETTVIPSLAEKWDV
SEDGKSYTFYLRKGVKFHSTKDFKPSRDFNADDVLFSFNRQLDPNHPYHKISGGNYEYFIGMDMGNIIQNVEKVDDYTVK
INLKVPNAPFLANIAMDFASIFSAEYADKLLKAGTPEKLDQNPIGTGPFQFVDYQKDAQIRYKGFDQYWAGKPAIDRLVF
AITPDASVRMAKLQKGECHAAPYPNPADLEALKKDPNINLMTKPGLNIGYLHFNTQKKPFDDVKVRQALNHAINKDAIIQ
SVYQGSGEKAKNPIPPTMWSYNDDVKDYDYNPEKAKALLKEAGLEKGFDTEVWAMPVSRPYNPNARRMAELIQEDWKKVG
VNAKIVSYEWGEYLNRMRDGEHTTGMMGWNGDNGDPDNFLNTLLSCAAVKQGSNYAKFCHKDFDKLMTDAVQTTDKAKRT
ELYKQAQVLFKEQAPWVTIAHSTTYFPVRKEVKGYVISPFSLHNFYGVDLEAK

Sequences:

>Translated_533_residues
MKKLTKLSLLSLAIAASSSALAAPKTFVYCLEASPTYLNPQFATDGGTLDATSQAIFNRLVDFAKGETTVIPSLAEKWDV
SEDGKSYTFYLRKGVKFHSTKDFKPSRDFNADDVLFSFNRQLDPNHPYHKISGGNYEYFIGMDMGNIIQNVEKVDDYTVK
INLKVPNAPFLANIAMDFASIFSAEYADKLLKAGTPEKLDQNPIGTGPFQFVDYQKDAQIRYKGFDQYWAGKPAIDRLVF
AITPDASVRMAKLQKGECHAAPYPNPADLEALKKDPNINLMTKPGLNIGYLHFNTQKKPFDDVKVRQALNHAINKDAIIQ
SVYQGSGEKAKNPIPPTMWSYNDDVKDYDYNPEKAKALLKEAGLEKGFDTEVWAMPVSRPYNPNARRMAELIQEDWKKVG
VNAKIVSYEWGEYLNRMRDGEHTTGMMGWNGDNGDPDNFLNTLLSCAAVKQGSNYAKFCHKDFDKLMTDAVQTTDKAKRT
ELYKQAQVLFKEQAPWVTIAHSTTYFPVRKEVKGYVISPFSLHNFYGVDLEAK
>Mature_533_residues
MKKLTKLSLLSLAIAASSSALAAPKTFVYCLEASPTYLNPQFATDGGTLDATSQAIFNRLVDFAKGETTVIPSLAEKWDV
SEDGKSYTFYLRKGVKFHSTKDFKPSRDFNADDVLFSFNRQLDPNHPYHKISGGNYEYFIGMDMGNIIQNVEKVDDYTVK
INLKVPNAPFLANIAMDFASIFSAEYADKLLKAGTPEKLDQNPIGTGPFQFVDYQKDAQIRYKGFDQYWAGKPAIDRLVF
AITPDASVRMAKLQKGECHAAPYPNPADLEALKKDPNINLMTKPGLNIGYLHFNTQKKPFDDVKVRQALNHAINKDAIIQ
SVYQGSGEKAKNPIPPTMWSYNDDVKDYDYNPEKAKALLKEAGLEKGFDTEVWAMPVSRPYNPNARRMAELIQEDWKKVG
VNAKIVSYEWGEYLNRMRDGEHTTGMMGWNGDNGDPDNFLNTLLSCAAVKQGSNYAKFCHKDFDKLMTDAVQTTDKAKRT
ELYKQAQVLFKEQAPWVTIAHSTTYFPVRKEVKGYVISPFSLHNFYGVDLEAK

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=525, Percent_Identity=66.4761904761905, Blast_Score=741, Evalue=0.0,
Organism=Escherichia coli, GI1787551, Length=531, Percent_Identity=37.2881355932203, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1787052, Length=537, Percent_Identity=29.7951582867784, Blast_Score=212, Evalue=6e-56,
Organism=Escherichia coli, GI1787762, Length=538, Percent_Identity=27.8810408921933, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1789887, Length=548, Percent_Identity=24.6350364963504, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1789397, Length=541, Percent_Identity=27.3567467652495, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1787495, Length=466, Percent_Identity=27.2532188841202, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081878, Length=541, Percent_Identity=26.0628465804067, Blast_Score=115, Evalue=9e-27,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60004; Mature: 60004

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTKLSLLSLAIAASSSALAAPKTFVYCLEASPTYLNPQFATDGGTLDATSQAIFNRL
CCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHHHHHHHHH
VDFAKGETTVIPSLAEKWDVSEDGKSYTFYLRKGVKFHSTKDFKPSRDFNADDVLFSFNR
HHHHCCCEEECCHHHHHCCCCCCCCEEEEEEECCCEECCCCCCCCCCCCCCCCEEEEECC
QLDPNHPYHKISGGNYEYFIGMDMGNIIQNVEKVDDYTVKINLKVPNAPFLANIAMDFAS
CCCCCCCCEECCCCCEEEEEECCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHH
IFSAEYADKLLKAGTPEKLDQNPIGTGPFQFVDYQKDAQIRYKGFDQYWAGKPAIDRLVF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCHHHCCCCCCHHCEEE
AITPDASVRMAKLQKGECHAAPYPNPADLEALKKDPNINLMTKPGLNIGYLHFNTQKKPF
EECCCCCEEHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCEEEEEEECCCCCCC
DDVKVRQALNHAINKDAIIQSVYQGSGEKAKNPIPPTMWSYNDDVKDYDYNPEKAKALLK
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EAGLEKGFDTEVWAMPVSRPYNPNARRMAELIQEDWKKVGVNAKIVSYEWGEYLNRMRDG
HHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHCCC
EHTTGMMGWNGDNGDPDNFLNTLLSCAAVKQGSNYAKFCHKDFDKLMTDAVQTTDKAKRT
CCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELYKQAQVLFKEQAPWVTIAHSTTYFPVRKEVKGYVISPFSLHNFYGVDLEAK
HHHHHHHHHHHCCCCEEEEECCCEECCCHHCCCCEEECCEEECCEECCEEECC
>Mature Secondary Structure
MKKLTKLSLLSLAIAASSSALAAPKTFVYCLEASPTYLNPQFATDGGTLDATSQAIFNRL
CCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHHHHHHHHH
VDFAKGETTVIPSLAEKWDVSEDGKSYTFYLRKGVKFHSTKDFKPSRDFNADDVLFSFNR
HHHHCCCEEECCHHHHHCCCCCCCCEEEEEEECCCEECCCCCCCCCCCCCCCCEEEEECC
QLDPNHPYHKISGGNYEYFIGMDMGNIIQNVEKVDDYTVKINLKVPNAPFLANIAMDFAS
CCCCCCCCEECCCCCEEEEEECCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHH
IFSAEYADKLLKAGTPEKLDQNPIGTGPFQFVDYQKDAQIRYKGFDQYWAGKPAIDRLVF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCHHHCCCCCCHHCEEE
AITPDASVRMAKLQKGECHAAPYPNPADLEALKKDPNINLMTKPGLNIGYLHFNTQKKPF
EECCCCCEEHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCEEEEEEECCCCCCC
DDVKVRQALNHAINKDAIIQSVYQGSGEKAKNPIPPTMWSYNDDVKDYDYNPEKAKALLK
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EAGLEKGFDTEVWAMPVSRPYNPNARRMAELIQEDWKKVGVNAKIVSYEWGEYLNRMRDG
HHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHCCC
EHTTGMMGWNGDNGDPDNFLNTLLSCAAVKQGSNYAKFCHKDFDKLMTDAVQTTDKAKRT
CCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELYKQAQVLFKEQAPWVTIAHSTTYFPVRKEVKGYVISPFSLHNFYGVDLEAK
HHHHHHHHHHHCCCCEEEEECCCEECCCHHCCCCEEECCEEECCEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]