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The map label for this gene is lpxB

Identifier: 126207495

GI number: 126207495

Start: 7413

End: 8594

Strand: Direct

Name: lpxB

Synonym: APL_0007

Alternate gene names: 126207495

Gene position: 7413-8594 (Clockwise)

Preceding gene: 126207493

Following gene: 126207496

Centisome position: 0.33

GC content: 43.99

Gene sequence:

>1182_bases
ATGATGACAAAAGAAGCGCCTCTTATTGCTCTAGTTGCCGGTGAGATTTCTGGTGATATTCTTGGTGCTGGGTTGATTAA
CGCCCTTAAATTACATTATCCGAACGCCCGTTTTATCGGAGTTGCCGGCCCCCGAATGATACAAGCCGGTTGTGAAACGC
TCTTCGATATGGAAGAACTGGCGGTAATGGGATTGGCGGAAGTGGTCAAACATTTGCCTCGTTTGCTTAAGCGTCGTAAA
CAAGTGATTGAAACCATGCTTGCGGAAAAGCCGGATATTTTTATCGGTATTGATGCGCCGGATTTTAATTTAACCGTTGA
AGAAAAACTCAAAGCGAGCGGAATTAAAACCATTCATTATGTCAGTCCGTCGGTGTGGGCGTGGCGACAAAATCGAGTAC
ATAAAATCGCACGGGCAACCAACTTAGTGTTGGCTTTTTTACCGTTTGAAAAAGCGTTTTATGATCGCTTTAATGTGCCT
TGTCGTTTTATCGGACATACGATGGCGGATACGATTGCGCTTAAGCCGAATCGTGCGGAAGCCTGTGTCGGTTTAAATTT
GGACGAAGCACAACGTTACTTGGCGATTTTAGTCGGCAGTCGGGCGAGCGAAGTCGGCTTTTTAGCGGAACCTTTCTTAA
AAGCGGCGCAAATTCTAAAACAACAGTATCCGGACTTACAATTTTTAGTGCCGTTGGTTAATGACAAGCGTATCGCCCAA
TTCGAGCAAATTAAAGCGCAAGTTGCTCCTGAATTATCGGTACATATTCTGAAAGGCAACGCCCGTCAAGCGATGATTGC
GGCGGAAGCCTCATTACTTGCTTCCGGTACGGCGGCATTAGAGGGAATGTTATGCAAATCACCGATGGTTGTCGGCTATA
AAATGAAAGCGATGACTTATTGGTTGGCGAAACGCTTAGTGAAGACCAAATATATTTCCCTGCCTAATTTATTAGCGGAT
GAAATGCTCGTGCCGGAATTAATTCAAGACGAATGTAATCCAGAAAATTTAGCTTGGTATTTGGGTAATTATTTGGCGGA
TGATGCAGACCATAGAAAACAGCGAAACGAATTAAAACAACGTTTTACTGAATTACATAAACTGATTCAATGTGATGCGG
ACGCTCAAGCGGCTCAAGCGGTTGTCGATGTATTGGAAGCGAACACAAGCGATCAAAATTAA

Upstream 100 bases:

>100_bases
GAAAAATTCAATCAATGTGATTTTGTTAGTTTATATTGTGATTGGTTATAATCAGCCTATAATAAAAGTAATTAAATGAA
TAATTAATGAGAGTGAAATT

Downstream 100 bases:

>100_bases
TTTTATTTTTACAAAAGGAATCTCACGGCATGTGGAAAGAAATTTTACTTAATTACGGTATTTTTTTATTAGAATTACTG
ACGTTGTTCGGCATTGTTGC

Product: lipid-A-disaccharide synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MMTKEAPLIALVAGEISGDILGAGLINALKLHYPNARFIGVAGPRMIQAGCETLFDMEELAVMGLAEVVKHLPRLLKRRK
QVIETMLAEKPDIFIGIDAPDFNLTVEEKLKASGIKTIHYVSPSVWAWRQNRVHKIARATNLVLAFLPFEKAFYDRFNVP
CRFIGHTMADTIALKPNRAEACVGLNLDEAQRYLAILVGSRASEVGFLAEPFLKAAQILKQQYPDLQFLVPLVNDKRIAQ
FEQIKAQVAPELSVHILKGNARQAMIAAEASLLASGTAALEGMLCKSPMVVGYKMKAMTYWLAKRLVKTKYISLPNLLAD
EMLVPELIQDECNPENLAWYLGNYLADDADHRKQRNELKQRFTELHKLIQCDADAQAAQAVVDVLEANTSDQN

Sequences:

>Translated_393_residues
MMTKEAPLIALVAGEISGDILGAGLINALKLHYPNARFIGVAGPRMIQAGCETLFDMEELAVMGLAEVVKHLPRLLKRRK
QVIETMLAEKPDIFIGIDAPDFNLTVEEKLKASGIKTIHYVSPSVWAWRQNRVHKIARATNLVLAFLPFEKAFYDRFNVP
CRFIGHTMADTIALKPNRAEACVGLNLDEAQRYLAILVGSRASEVGFLAEPFLKAAQILKQQYPDLQFLVPLVNDKRIAQ
FEQIKAQVAPELSVHILKGNARQAMIAAEASLLASGTAALEGMLCKSPMVVGYKMKAMTYWLAKRLVKTKYISLPNLLAD
EMLVPELIQDECNPENLAWYLGNYLADDADHRKQRNELKQRFTELHKLIQCDADAQAAQAVVDVLEANTSDQN
>Mature_393_residues
MMTKEAPLIALVAGEISGDILGAGLINALKLHYPNARFIGVAGPRMIQAGCETLFDMEELAVMGLAEVVKHLPRLLKRRK
QVIETMLAEKPDIFIGIDAPDFNLTVEEKLKASGIKTIHYVSPSVWAWRQNRVHKIARATNLVLAFLPFEKAFYDRFNVP
CRFIGHTMADTIALKPNRAEACVGLNLDEAQRYLAILVGSRASEVGFLAEPFLKAAQILKQQYPDLQFLVPLVNDKRIAQ
FEQIKAQVAPELSVHILKGNARQAMIAAEASLLASGTAALEGMLCKSPMVVGYKMKAMTYWLAKRLVKTKYISLPNLLAD
EMLVPELIQDECNPENLAWYLGNYLADDADHRKQRNELKQRFTELHKLIQCDADAQAAQAVVDVLEANTSDQN

Specific function: Condensation of UDP-2,3-diacylglucosamine and 2,3- diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell

COG id: COG0763

COG function: function code M; Lipid A disaccharide synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lpxB family

Homologues:

Organism=Escherichia coli, GI1786379, Length=384, Percent_Identity=62.5, Blast_Score=466, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXB_ACTP2 (A3MY79)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052720.1
- ProteinModelPortal:   A3MY79
- STRING:   A3MY79
- GeneID:   4850465
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0007
- eggNOG:   COG0763
- HOGENOM:   HBG584837
- OMA:   DFTHRVA
- PhylomeDB:   A3MY79
- ProtClustDB:   PRK00025
- BioCyc:   APLE416269:APL_0007-MONOMER
- HAMAP:   MF_00392
- InterPro:   IPR003835
- TIGRFAMs:   TIGR00215

Pfam domain/function: PF02684 LpxB

EC number: =2.4.1.182

Molecular weight: Translated: 43750; Mature: 43750

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTKEAPLIALVAGEISGDILGAGLINALKLHYPNARFIGVAGPRMIQAGCETLFDMEEL
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEECCCHHHHHHHHHHHHHHHHH
AVMGLAEVVKHLPRLLKRRKQVIETMLAEKPDIFIGIDAPDFNLTVEEKLKASGIKTIHY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEE
VSPSVWAWRQNRVHKIARATNLVLAFLPFEKAFYDRFNVPCRFIGHTMADTIALKPNRAE
CCCCHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCC
ACVGLNLDEAQRYLAILVGSRASEVGFLAEPFLKAAQILKQQYPDLQFLVPLVNDKRIAQ
CEECCCHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHH
FEQIKAQVAPELSVHILKGNARQAMIAAEASLLASGTAALEGMLCKSPMVVGYKMKAMTY
HHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEHHHHHHH
WLAKRLVKTKYISLPNLLADEMLVPELIQDECNPENLAWYLGNYLADDADHRKQRNELKQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
RFTELHKLIQCDADAQAAQAVVDVLEANTSDQN
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMTKEAPLIALVAGEISGDILGAGLINALKLHYPNARFIGVAGPRMIQAGCETLFDMEEL
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEECCCHHHHHHHHHHHHHHHHH
AVMGLAEVVKHLPRLLKRRKQVIETMLAEKPDIFIGIDAPDFNLTVEEKLKASGIKTIHY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEE
VSPSVWAWRQNRVHKIARATNLVLAFLPFEKAFYDRFNVPCRFIGHTMADTIALKPNRAE
CCCCHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCC
ACVGLNLDEAQRYLAILVGSRASEVGFLAEPFLKAAQILKQQYPDLQFLVPLVNDKRIAQ
CEECCCHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHH
FEQIKAQVAPELSVHILKGNARQAMIAAEASLLASGTAALEGMLCKSPMVVGYKMKAMTY
HHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEHHHHHHH
WLAKRLVKTKYISLPNLLADEMLVPELIQDECNPENLAWYLGNYLADDADHRKQRNELKQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
RFTELHKLIQCDADAQAAQAVVDVLEANTSDQN
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA