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The map label for this gene is phoB [H]

Identifier: 126175726

GI number: 126175726

Start: 4148666

End: 4149943

Strand: Direct

Name: phoB [H]

Synonym: Sbal_3529

Alternate gene names: 126175726

Gene position: 4148666-4149943 (Clockwise)

Preceding gene: 126175724

Following gene: 126175727

Centisome position: 80.91

GC content: 50.23

Gene sequence:

>1278_bases
TTGCTGTTATCTACGCCAGTTTGGGCAGATGAAACGGGTCCAACGAAAGCGCCATCGCGACCGAAAAATATCGTCATTAT
GATAGGTGATGGTATGGGGCCGTCTTACACCAGCGCCTACCGCTATTACAAAGATAATCCTGACACTGAAGAAGTCGAGC
AAACCGTATTTGACCGTTTACTGGTGGGCATGGCGAGCACCTATCCCGCGAGCGTCAGTGGTTATGTGACTGACTCAGCC
GCCGCGGCAACCGCACTCGCCACTGGGGTAAAATCGTATAATGGGGCGATTTCGGTCGATACCCAAAAGCAACCACTGCC
GACCATACTCGAAAAAGCCAAATCCCTTGGCCTCAGCACAGGTGTCGCGGTAACGTCGCAAATCAACCACGCCACACCAG
CCGCCTTTTTGGCCCACAACGAGAGCCGTAAAAACTACGACGCTCTGGCACTCAGCTATTTAGAAACCAACGCCGATGTA
TTTTTAGGCGGTGGTCAAAAATATTTCCCCCCTGCGCTATTAGAGCAGTTCAAAGCCAAGGGCTATCAACACATCAGCCG
TTTTGAGGATCTCGCCAGCATCACCCAACCAAAAGTGTTGGGCCTGTTTGCCGAAGTACAATTGCCTTGGGCGATTGATG
AGAAAGATGCAAGAAAACTCAGCACCTTAACTCAAAAGGCACTCAGCTTACTGTCGCAAAATGACCAAGGTTTTGTGTTG
TTAGTCGAAGGGAGTCTCATCGACTGGGCGGGCCACAATAACGATATCGCCACTGCCATGGGTGAGATGGATGAATTCTC
CAATGCCATTGAAGTGGTTGAGCAATTTGTGCGTGAACATCCAGATACCCTGATGGTGATCACCGCCGACCACAATACCG
GTGGCCTATCGATTGGCGTCGATGGCAACTACAGCTGGAACCCTGAAATCCTGCGTAATATCTCGGCCAGCACAGACACT
TTGGCCTTAGCCGCCATCACTGGTGAACAATGGCAAGCCGATCTCGCCCGTGGTTTAGGCTTTGAGCTTAGCGAAGAAGA
AGTCGCAAAATTAAACGTCGCCAGAATGCAGGGATTAGAAACCATGGCAGTAGCGATTCGCCATCTTATTGATAAACGTA
CTGATACAGGTTGGACAACCGACGGCCACACAGGCACAGACGTACAAGTGTTTGCCGCAGGTCCGGCGTCTGAGCTGTTT
AACGGCCATCAGGACAATACCGATATCGCCAATAAGATTTTTAGCTTATTGCCAAAACCGAAGAAACCTAAAAGCTAA

Upstream 100 bases:

>100_bases
GTGAGGTTTTGACACGGTTGATGGGTAAGTTGATAGGCGATGCCGATCAGATCTGCGGCTACGGTTTTAGTTCAACCTAT
TTAGGAAACAAGGCCTCAGT

Downstream 100 bases:

>100_bases
GCGTTAATCGATACCGTTCATTAAAGGCAACCGCATAATGCAGTTGCCTTTTTTATTGGGTCTTTTTATTAGGGATTTTA
TTAAGTCTTAGCGCAGGGAT

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MLLSTPVWADETGPTKAPSRPKNIVIMIGDGMGPSYTSAYRYYKDNPDTEEVEQTVFDRLLVGMASTYPASVSGYVTDSA
AAATALATGVKSYNGAISVDTQKQPLPTILEKAKSLGLSTGVAVTSQINHATPAAFLAHNESRKNYDALALSYLETNADV
FLGGGQKYFPPALLEQFKAKGYQHISRFEDLASITQPKVLGLFAEVQLPWAIDEKDARKLSTLTQKALSLLSQNDQGFVL
LVEGSLIDWAGHNNDIATAMGEMDEFSNAIEVVEQFVREHPDTLMVITADHNTGGLSIGVDGNYSWNPEILRNISASTDT
LALAAITGEQWQADLARGLGFELSEEEVAKLNVARMQGLETMAVAIRHLIDKRTDTGWTTDGHTGTDVQVFAAGPASELF
NGHQDNTDIANKIFSLLPKPKKPKS

Sequences:

>Translated_425_residues
MLLSTPVWADETGPTKAPSRPKNIVIMIGDGMGPSYTSAYRYYKDNPDTEEVEQTVFDRLLVGMASTYPASVSGYVTDSA
AAATALATGVKSYNGAISVDTQKQPLPTILEKAKSLGLSTGVAVTSQINHATPAAFLAHNESRKNYDALALSYLETNADV
FLGGGQKYFPPALLEQFKAKGYQHISRFEDLASITQPKVLGLFAEVQLPWAIDEKDARKLSTLTQKALSLLSQNDQGFVL
LVEGSLIDWAGHNNDIATAMGEMDEFSNAIEVVEQFVREHPDTLMVITADHNTGGLSIGVDGNYSWNPEILRNISASTDT
LALAAITGEQWQADLARGLGFELSEEEVAKLNVARMQGLETMAVAIRHLIDKRTDTGWTTDGHTGTDVQVFAAGPASELF
NGHQDNTDIANKIFSLLPKPKKPKS
>Mature_425_residues
MLLSTPVWADETGPTKAPSRPKNIVIMIGDGMGPSYTSAYRYYKDNPDTEEVEQTVFDRLLVGMASTYPASVSGYVTDSA
AAATALATGVKSYNGAISVDTQKQPLPTILEKAKSLGLSTGVAVTSQINHATPAAFLAHNESRKNYDALALSYLETNADV
FLGGGQKYFPPALLEQFKAKGYQHISRFEDLASITQPKVLGLFAEVQLPWAIDEKDARKLSTLTQKALSLLSQNDQGFVL
LVEGSLIDWAGHNNDIATAMGEMDEFSNAIEVVEQFVREHPDTLMVITADHNTGGLSIGVDGNYSWNPEILRNISASTDT
LALAAITGEQWQADLARGLGFELSEEEVAKLNVARMQGLETMAVAIRHLIDKRTDTGWTTDGHTGTDVQVFAAGPASELF
NGHQDNTDIANKIFSLLPKPKKPKS

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=454, Percent_Identity=29.9559471365639, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI116734717, Length=456, Percent_Identity=28.2894736842105, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI94721246, Length=452, Percent_Identity=28.7610619469027, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI294660770, Length=452, Percent_Identity=28.0973451327434, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI157266292, Length=452, Percent_Identity=28.0973451327434, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI48994877, Length=328, Percent_Identity=35.6707317073171, Blast_Score=162, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320689, Length=435, Percent_Identity=31.264367816092, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21355981, Length=449, Percent_Identity=27.8396436525612, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24651554, Length=463, Percent_Identity=29.8056155507559, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI19921912, Length=460, Percent_Identity=26.9565217391304, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI85725200, Length=444, Percent_Identity=26.5765765765766, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI85815833, Length=444, Percent_Identity=26.5765765765766, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24657842, Length=449, Percent_Identity=27.3942093541203, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859923, Length=293, Percent_Identity=28.6689419795222, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21358067, Length=449, Percent_Identity=27.3942093541203, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21355151, Length=448, Percent_Identity=25.2232142857143, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24657835, Length=448, Percent_Identity=27.0089285714286, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24657827, Length=445, Percent_Identity=26.0674157303371, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 45938; Mature: 45938

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSTPVWADETGPTKAPSRPKNIVIMIGDGMGPSYTSAYRYYKDNPDTEEVEQTVFDRL
CCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHEECCCCCHHHHHHHHHHHH
LVGMASTYPASVSGYVTDSAAAATALATGVKSYNGAISVDTQKQPLPTILEKAKSLGLST
HHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCC
GVAVTSQINHATPAAFLAHNESRKNYDALALSYLETNADVFLGGGQKYFPPALLEQFKAK
CEEEECCCCCCCCHHHEECCCCCCCHHHHHHHEEECCCCEEECCCCCCCCHHHHHHHHHH
GYQHISRFEDLASITQPKVLGLFAEVQLPWAIDEKDARKLSTLTQKALSLLSQNDQGFVL
HHHHHHHHHHHHHHCCCHHHEEEEEEECCEEECHHHHHHHHHHHHHHHHHHCCCCCCEEE
LVEGSLIDWAGHNNDIATAMGEMDEFSNAIEVVEQFVREHPDTLMVITADHNTGGLSIGV
EEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEE
DGNYSWNPEILRNISASTDTLALAAITGEQWQADLARGLGFELSEEEVAKLNVARMQGLE
CCCCCCCHHHHHHCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHH
TMAVAIRHLIDKRTDTGWTTDGHTGTDVQVFAAGPASELFNGHQDNTDIANKIFSLLPKP
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCCHHHHHHHHHHCCCC
KKPKS
CCCCC
>Mature Secondary Structure
MLLSTPVWADETGPTKAPSRPKNIVIMIGDGMGPSYTSAYRYYKDNPDTEEVEQTVFDRL
CCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHEECCCCCHHHHHHHHHHHH
LVGMASTYPASVSGYVTDSAAAATALATGVKSYNGAISVDTQKQPLPTILEKAKSLGLST
HHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCC
GVAVTSQINHATPAAFLAHNESRKNYDALALSYLETNADVFLGGGQKYFPPALLEQFKAK
CEEEECCCCCCCCHHHEECCCCCCCHHHHHHHEEECCCCEEECCCCCCCCHHHHHHHHHH
GYQHISRFEDLASITQPKVLGLFAEVQLPWAIDEKDARKLSTLTQKALSLLSQNDQGFVL
HHHHHHHHHHHHHHCCCHHHEEEEEEECCEEECHHHHHHHHHHHHHHHHHHCCCCCCEEE
LVEGSLIDWAGHNNDIATAMGEMDEFSNAIEVVEQFVREHPDTLMVITADHNTGGLSIGV
EEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEE
DGNYSWNPEILRNISASTDTLALAAITGEQWQADLARGLGFELSEEEVAKLNVARMQGLE
CCCCCCCHHHHHHCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHH
TMAVAIRHLIDKRTDTGWTTDGHTGTDVQVFAAGPASELFNGHQDNTDIANKIFSLLPKP
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCCHHHHHHHHHHCCCC
KKPKS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]