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The map label for this gene is gshB [H]

Identifier: 126175724

GI number: 126175724

Start: 4146529

End: 4147482

Strand: Direct

Name: gshB [H]

Synonym: Sbal_3527

Alternate gene names: 126175724

Gene position: 4146529-4147482 (Clockwise)

Preceding gene: 126175723

Following gene: 126175726

Centisome position: 80.87

GC content: 50.1

Gene sequence:

>954_bases
ATGATAAAACTCGGCATAGTGATGGACCCCATCAGTGAGATCAACATTAAGAAAGACTCCAGTTTTGCCATGTTAATGGC
CGCACAGGACAGAGGTTATCAGCTGTTTTATATGGAAATGGCCGATCTTGCCATGGTCAACGGCGTGGCGATGGCCAATA
TGCGTCCGTTAACGGTCATCAATGATGCGAGTAAATGGTATGAGCTAGGTGAATCTCAAGACACGCCAATGAGCGAACTC
GACGTGATCTTAATGCGTAAAGACCCGCCATTTGATACTGAGTTCATCTACGCCACTTACATGCTTGAGCGCGCCGAAGA
GCAAGGCGTGTTGATCGTCAACAAGCCGCAAAGCCTACGCGATGCTAACGAAAAGCTGTTCACCGCATGGTTTAGCGAAT
TCACCCCAGAAACAATCGTGACCCGTGATGCCAAACGTATCCGCGCTTTCCACCAAGCTAAGGGCGATATCATTCTCAAG
CCTTTAGATGGCATGGGCGGCACCTCGATTTTCCGCGTGAAGCAGGATGACCCTAACCTTGGGGTGATCATCGAGACCTT
GACCAGTTATGGCCAACAATATGCGATGGCGCAAGCCTTTATCCCTGAAATTACTAAAGGCGATAAACGCATTTTAGTGA
TCGATGGCGAGCCTGTTCCTTACGCGTTGGCACGTATCCCAATGAAAGGCGAAACCCGTGGCAACTTAGCCGCCGGCGGT
AGCGGTGTGGCGCAGCCTTTATCCGACAGCGATTGGGCAATTGCCCGCGCCATTGGCCCTGAGCTTAAAAAACGTGGACT
GATTTTTGTCGGACTAGACGTGATTGGTGACAAGCTGACTGAGATCAATGTGACGAGCCCAACCTGTATCCGTGAAATCC
AAGCGGCATTTGATGTCGACATCACGGGCATGTTAATGGATGCTATCGAAGCACGCATTAACGCCACAGCATAA

Upstream 100 bases:

>100_bases
TTAGGCTAAACCGCAGCCGCAAGCTGCGCTGGCATAACAGACCCCATTATCCAATAAATGCTTTTTTGCCTGCAAACGGC
GACAAATAAGGAACAGATTA

Downstream 100 bases:

>100_bases
GAATTTACCAACGAGTTTGTTGATTGTAAGGCGCGAGCAAATCGCGAGCGCCGAACCACTTCTAGGGATGTGATATGAGT
TTCACCAAAGCGCCACTCTT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MIKLGIVMDPISEINIKKDSSFAMLMAAQDRGYQLFYMEMADLAMVNGVAMANMRPLTVINDASKWYELGESQDTPMSEL
DVILMRKDPPFDTEFIYATYMLERAEEQGVLIVNKPQSLRDANEKLFTAWFSEFTPETIVTRDAKRIRAFHQAKGDIILK
PLDGMGGTSIFRVKQDDPNLGVIIETLTSYGQQYAMAQAFIPEITKGDKRILVIDGEPVPYALARIPMKGETRGNLAAGG
SGVAQPLSDSDWAIARAIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIREIQAAFDVDITGMLMDAIEARINATA

Sequences:

>Translated_317_residues
MIKLGIVMDPISEINIKKDSSFAMLMAAQDRGYQLFYMEMADLAMVNGVAMANMRPLTVINDASKWYELGESQDTPMSEL
DVILMRKDPPFDTEFIYATYMLERAEEQGVLIVNKPQSLRDANEKLFTAWFSEFTPETIVTRDAKRIRAFHQAKGDIILK
PLDGMGGTSIFRVKQDDPNLGVIIETLTSYGQQYAMAQAFIPEITKGDKRILVIDGEPVPYALARIPMKGETRGNLAAGG
SGVAQPLSDSDWAIARAIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIREIQAAFDVDITGMLMDAIEARINATA
>Mature_317_residues
MIKLGIVMDPISEINIKKDSSFAMLMAAQDRGYQLFYMEMADLAMVNGVAMANMRPLTVINDASKWYELGESQDTPMSEL
DVILMRKDPPFDTEFIYATYMLERAEEQGVLIVNKPQSLRDANEKLFTAWFSEFTPETIVTRDAKRIRAFHQAKGDIILK
PLDGMGGTSIFRVKQDDPNLGVIIETLTSYGQQYAMAQAFIPEITKGDKRILVIDGEPVPYALARIPMKGETRGNLAAGG
SGVAQPLSDSDWAIARAIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIREIQAAFDVDITGMLMDAIEARINATA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=314, Percent_Identity=71.3375796178344, Blast_Score=474, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35022; Mature: 35022

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLGIVMDPISEINIKKDSSFAMLMAAQDRGYQLFYMEMADLAMVNGVAMANMRPLTVI
CEEEEEEECCHHHCCCCCCCCEEEEEEECCCCEEEEEEEHHHHHHHCCEEECCCCCEEEE
NDASKWYELGESQDTPMSELDVILMRKDPPFDTEFIYATYMLERAEEQGVLIVNKPQSLR
CCCHHHHHCCCCCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHH
DANEKLFTAWFSEFTPETIVTRDAKRIRAFHQAKGDIILKPLDGMGGTSIFRVKQDDPNL
HHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCC
GVIIETLTSYGQQYAMAQAFIPEITKGDKRILVIDGEPVPYALARIPMKGETRGNLAAGG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCEECCC
SGVAQPLSDSDWAIARAIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIREIQAAFDVD
CCCCCCCCCCCCHHHHHHCHHHHHCCEEEEEHHHHCCCEEEEECCCHHHHHHHHHHHCCC
ITGMLMDAIEARINATA
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKLGIVMDPISEINIKKDSSFAMLMAAQDRGYQLFYMEMADLAMVNGVAMANMRPLTVI
CEEEEEEECCHHHCCCCCCCCEEEEEEECCCCEEEEEEEHHHHHHHCCEEECCCCCEEEE
NDASKWYELGESQDTPMSELDVILMRKDPPFDTEFIYATYMLERAEEQGVLIVNKPQSLR
CCCHHHHHCCCCCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHH
DANEKLFTAWFSEFTPETIVTRDAKRIRAFHQAKGDIILKPLDGMGGTSIFRVKQDDPNL
HHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCC
GVIIETLTSYGQQYAMAQAFIPEITKGDKRILVIDGEPVPYALARIPMKGETRGNLAAGG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCEECCC
SGVAQPLSDSDWAIARAIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIREIQAAFDVD
CCCCCCCCCCCCHHHHHHCHHHHHCCEEEEEHHHHCCCEEEEECCCHHHHHHHHHHHCCC
ITGMLMDAIEARINATA
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]