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The map label for this gene is htrB [H]

Identifier: 126175621

GI number: 126175621

Start: 4013272

End: 4014198

Strand: Direct

Name: htrB [H]

Synonym: Sbal_3424

Alternate gene names: 126175621

Gene position: 4013272-4014198 (Clockwise)

Preceding gene: 126175616

Following gene: 126175624

Centisome position: 78.27

GC content: 49.08

Gene sequence:

>927_bases
GTGGTCGAGAAAGCCGAGTTTTCATCTTCTTTATATCATCCTAAGCATTGGCCCATGTGGTTTGGCGTATTTTTAATGCG
CTTAACTCAGCTTCTACCGCTCTCTTGGCAGATGAAACTGGGTAAAGGTTTAGGTCGCTTAGTGATGAAATTTGCTGGCA
GTCGCACTCATACGGCACGCCGTAACTTGAGCCTCTGTTTCCCTGAAATGTCAGAAGCACAACGAGAAACCCTACTCACG
CGTAACTTTGAAGAAACGGGCAAAGCCATTTTCGATACCATCAATGCATGGTGGTGGTCCGATGAAAAAGTGCAGCAGCA
CATGAGCATCACAGGCAAAGAACACGTGCAAGACACCCTCGATGCTGGCCACGGCGTTATCCTGTTTGCCGTGCATTGTT
TACCGCTGGAAATGGGCGCACGTATCTTCGGCCAATTCCAACCCGGCGTGGGGGTTTATCGCCCACACAACAATCCTGTG
ATGGAATACCTCCAAGTTAAAGGCCGTTTACGCTCGAATAAAGGGCTTGTTTCTAAACGCGACTTGCGCCAAATGGTGCG
CTGCCTGCGCAATCCCGATGTCATTTGGTACACGGCCGATCAAGACTTTGGCCGATCCAGCGCGGTATTTATCCCGTTTT
ATGCCATGCCAGATGCGGCAACGATTACGGGGGCCACCACGTTAGCCAAACTCGGCAAAGCCAAAGTCTTACCCTTTTTT
GTGGAACGTACTGAAGGTGATGAGGGCTATCGGATTGAAGTGATGCCACCGCTGGATAATTTCCCCGGCGAAGATGAATT
AGCCGATGCCATTCGCGGTAACAAAATTATCGAATCCATCATAGATAAAAACCGCGCCCAATATATGTGGTTGCATCGCC
GCTTTAAAACCCGTCCAGATCCAACGGACAAATCTTTATATCAGTAA

Upstream 100 bases:

>100_bases
GCTCTTATCTTTATGCAGAAATTAAACCGTGATTGTACCTAATTTTCAGTAAAAATTGAGTTAGAATAAGCAGTAATTTT
CAAAAGTAGTGAGTATTTTT

Downstream 100 bases:

>100_bases
CTCATTCCGTTTTTGAATACAAAAATGCCAGCAAATAAGCTGGCATTTTTTATGGCGACTTTCGATTACAATCGTCGATG
ATAACTTTCAATGGCAATTA

Product: lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MVEKAEFSSSLYHPKHWPMWFGVFLMRLTQLLPLSWQMKLGKGLGRLVMKFAGSRTHTARRNLSLCFPEMSEAQRETLLT
RNFEETGKAIFDTINAWWWSDEKVQQHMSITGKEHVQDTLDAGHGVILFAVHCLPLEMGARIFGQFQPGVGVYRPHNNPV
MEYLQVKGRLRSNKGLVSKRDLRQMVRCLRNPDVIWYTADQDFGRSSAVFIPFYAMPDAATITGATTLAKLGKAKVLPFF
VERTEGDEGYRIEVMPPLDNFPGEDELADAIRGNKIIESIIDKNRAQYMWLHRRFKTRPDPTDKSLYQ

Sequences:

>Translated_308_residues
MVEKAEFSSSLYHPKHWPMWFGVFLMRLTQLLPLSWQMKLGKGLGRLVMKFAGSRTHTARRNLSLCFPEMSEAQRETLLT
RNFEETGKAIFDTINAWWWSDEKVQQHMSITGKEHVQDTLDAGHGVILFAVHCLPLEMGARIFGQFQPGVGVYRPHNNPV
MEYLQVKGRLRSNKGLVSKRDLRQMVRCLRNPDVIWYTADQDFGRSSAVFIPFYAMPDAATITGATTLAKLGKAKVLPFF
VERTEGDEGYRIEVMPPLDNFPGEDELADAIRGNKIIESIIDKNRAQYMWLHRRFKTRPDPTDKSLYQ
>Mature_308_residues
MVEKAEFSSSLYHPKHWPMWFGVFLMRLTQLLPLSWQMKLGKGLGRLVMKFAGSRTHTARRNLSLCFPEMSEAQRETLLT
RNFEETGKAIFDTINAWWWSDEKVQQHMSITGKEHVQDTLDAGHGVILFAVHCLPLEMGARIFGQFQPGVGVYRPHNNPV
MEYLQVKGRLRSNKGLVSKRDLRQMVRCLRNPDVIWYTADQDFGRSSAVFIPFYAMPDAATITGATTLAKLGKAKVLPFF
VERTEGDEGYRIEVMPPLDNFPGEDELADAIRGNKIIESIIDKNRAQYMWLHRRFKTRPDPTDKSLYQ

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=300, Percent_Identity=41.3333333333333, Blast_Score=274, Evalue=7e-75,
Organism=Escherichia coli, GI87082094, Length=307, Percent_Identity=37.1335504885993, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1788161, Length=295, Percent_Identity=28.4745762711864, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35439; Mature: 35439

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKAEFSSSLYHPKHWPMWFGVFLMRLTQLLPLSWQMKLGKGLGRLVMKFAGSRTHTAR
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
RNLSLCFPEMSEAQRETLLTRNFEETGKAIFDTINAWWWSDEKVQQHMSITGKEHVQDTL
CCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHH
DAGHGVILFAVHCLPLEMGARIFGQFQPGVGVYRPHNNPVMEYLQVKGRLRSNKGLVSKR
HCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCHH
DLRQMVRCLRNPDVIWYTADQDFGRSSAVFIPFYAMPDAATITGATTLAKLGKAKVLPFF
HHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHE
VERTEGDEGYRIEVMPPLDNFPGEDELADAIRGNKIIESIIDKNRAQYMWLHRRFKTRPD
EEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
PTDKSLYQ
CCCCCCCC
>Mature Secondary Structure
MVEKAEFSSSLYHPKHWPMWFGVFLMRLTQLLPLSWQMKLGKGLGRLVMKFAGSRTHTAR
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
RNLSLCFPEMSEAQRETLLTRNFEETGKAIFDTINAWWWSDEKVQQHMSITGKEHVQDTL
CCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHH
DAGHGVILFAVHCLPLEMGARIFGQFQPGVGVYRPHNNPVMEYLQVKGRLRSNKGLVSKR
HCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCHH
DLRQMVRCLRNPDVIWYTADQDFGRSSAVFIPFYAMPDAATITGATTLAKLGKAKVLPFF
HHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHE
VERTEGDEGYRIEVMPPLDNFPGEDELADAIRGNKIIESIIDKNRAQYMWLHRRFKTRPD
EEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
PTDKSLYQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]