Click here to switch to the map view.

The map label for this gene is pntA [H]

Identifier: 126175614

GI number: 126175614

Start: 4005722

End: 4007248

Strand: Direct

Name: pntA [H]

Synonym: Sbal_3417

Alternate gene names: 126175614

Gene position: 4005722-4007248 (Clockwise)

Preceding gene: 126175608

Following gene: 126175615

Centisome position: 78.12

GC content: 52.0

Gene sequence:

>1527_bases
ATGCAGATTGGAATACCGAGAGAAAGTCTCGCCGGTGAAACTAGGGTCGCCGCGACTCCAGCCACCGTCGAGCAGCTTAA
AAAACTCGGCTTTGAAGTTGTTATTGAGGCCAATGCGGGTCAACTGTCAAGTTTCGATGATGCAGCCTTTGAAGCTGCAG
GCGCGAAAGTGACTACTGACGTTTGGCAAGCCGATCTGATTTTTAAGGTCAATGCGCCAAGCGACGCCGAAATCGAACAA
ATCAAAGACGGAGCAACTGTCGTCAGCTTTATTTGGCCCGCACAAAACCCTGAGCTGGTTGAGAAGTTATCTAAACGCAA
AATTAACGTGATGGCGATGGACATGGTGCCTCGCATCTCCCGTGCCCAGTCACTCGATGCTCTCTCCTCTATGGCCAACA
TTGGCGGTTATCGTGCCGTTGTTGAAGCCGCGCATCAATTTGGCCGTTTCTTTACCGGTCAAATTACCGCTGCCGGTAAA
GTGCCGCCCGCTAAAGTGCTAGTGATTGGTGCCGGTGTTGCAGGCCTTGCCGCCATTGGTACTGCTGGTTCGCTTGGCGC
CATAGTGCGCGCCTTCGATACGCGCTTAGAAGTGGCCGAGCAAATCGAATCCATGGGCGGCCAGTTCCTTAAACTGGATT
TTGGTAACGAAGAAGGTGGCTCATCCGATGGTTATGCTAAAACCATGTCGGATGAATTTATTGCCGCCGAAATGGCGCTG
TTTGCTGAGCAAGCCAAAGAAGTCGATATCATCATCACTACGGCGCTGATCCCTGGTCGTCCTGCGCCTAAGCTCATCAC
CAAAGCTATGGTCGATAGCATGAAGAGCGGCTCAGTGATCGTCGATTTAGCCGCTGCAACTGGCGGTAACTGCGAATACA
CGCAATCGGGCGAGCTGTTTGTCACCCCGAACGGCGTAAAAGTGATTGGTTATACCGATCTACCCGGTCGTCTGCCCGCG
CAATCGTCACAGCTTTACGGCACTAACTTAGTCAATCTGATGAAGTTAATGTGTAAAGAGAAAGACGGTACTGCCAGCAT
CAACTTCGATGATGTGGTCATGCGCAACATGACTGTGATTAAAGAAGGTGAAGTGACCTTCCCGCCACCACCGATTTCGG
TTTCTGCGGCGCCAGCAAAACCTGCCGCAAAAATCGCGCCTAAAGACACCACCCCGAAAGCGCCATCTAAGCTCAAATAC
GTGCTCGCCGCCTTAGGCGTTGCTGGATTTGCAGCCGTGGCATCGGTTGCTCCGCCAGAGTTCCTGTCCCATTTCACGGT
ATTCTTGCTGTCCTGCGTCGTGGGTTATTATGTGGTGTGGAACGTATCTCACTCACTGCATACGCCTTTGATGTCAGTCA
CTAACGCGATTTCGGGCATTATCGTGGTCGGCGCCCTACTGCAAATCGGTCAGGGTTCAACGTTGGTCACTGTACTGGCG
TTTATCGCCGTGCTGATCGCCAGTATTAACATCTTCGGTGGCTTTACCGTCACTCAGCGCATGCTGAAGATGTTCCGTAA
GGATTAA

Upstream 100 bases:

>100_bases
CACCAGCCGAGTAATCGCTGGCGATGACAGATTTTGCAGTTGAGTACGATGAAGGAAGCACAGCAGGCGGAACGATTATA
TAAACCATCAGGGAGAAGTC

Downstream 100 bases:

>100_bases
GGGGGTTTAACGTGTCTCAAGGACTGGTAACAGCAGCTTACATTATTGCCGCCATTCTCTTCATCTTCAGTCTCGCGGGA
CTGTCGAAGCAAGAGACGGC

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVVIEANAGQLSSFDDAAFEAAGAKVTTDVWQADLIFKVNAPSDAEIEQ
IKDGATVVSFIWPAQNPELVEKLSKRKINVMAMDMVPRISRAQSLDALSSMANIGGYRAVVEAAHQFGRFFTGQITAAGK
VPPAKVLVIGAGVAGLAAIGTAGSLGAIVRAFDTRLEVAEQIESMGGQFLKLDFGNEEGGSSDGYAKTMSDEFIAAEMAL
FAEQAKEVDIIITTALIPGRPAPKLITKAMVDSMKSGSVIVDLAAATGGNCEYTQSGELFVTPNGVKVIGYTDLPGRLPA
QSSQLYGTNLVNLMKLMCKEKDGTASINFDDVVMRNMTVIKEGEVTFPPPPISVSAAPAKPAAKIAPKDTTPKAPSKLKY
VLAALGVAGFAAVASVAPPEFLSHFTVFLLSCVVGYYVVWNVSHSLHTPLMSVTNAISGIIVVGALLQIGQGSTLVTVLA
FIAVLIASINIFGGFTVTQRMLKMFRKD

Sequences:

>Translated_508_residues
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVVIEANAGQLSSFDDAAFEAAGAKVTTDVWQADLIFKVNAPSDAEIEQ
IKDGATVVSFIWPAQNPELVEKLSKRKINVMAMDMVPRISRAQSLDALSSMANIGGYRAVVEAAHQFGRFFTGQITAAGK
VPPAKVLVIGAGVAGLAAIGTAGSLGAIVRAFDTRLEVAEQIESMGGQFLKLDFGNEEGGSSDGYAKTMSDEFIAAEMAL
FAEQAKEVDIIITTALIPGRPAPKLITKAMVDSMKSGSVIVDLAAATGGNCEYTQSGELFVTPNGVKVIGYTDLPGRLPA
QSSQLYGTNLVNLMKLMCKEKDGTASINFDDVVMRNMTVIKEGEVTFPPPPISVSAAPAKPAAKIAPKDTTPKAPSKLKY
VLAALGVAGFAAVASVAPPEFLSHFTVFLLSCVVGYYVVWNVSHSLHTPLMSVTNAISGIIVVGALLQIGQGSTLVTVLA
FIAVLIASINIFGGFTVTQRMLKMFRKD
>Mature_508_residues
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVVIEANAGQLSSFDDAAFEAAGAKVTTDVWQADLIFKVNAPSDAEIEQ
IKDGATVVSFIWPAQNPELVEKLSKRKINVMAMDMVPRISRAQSLDALSSMANIGGYRAVVEAAHQFGRFFTGQITAAGK
VPPAKVLVIGAGVAGLAAIGTAGSLGAIVRAFDTRLEVAEQIESMGGQFLKLDFGNEEGGSSDGYAKTMSDEFIAAEMAL
FAEQAKEVDIIITTALIPGRPAPKLITKAMVDSMKSGSVIVDLAAATGGNCEYTQSGELFVTPNGVKVIGYTDLPGRLPA
QSSQLYGTNLVNLMKLMCKEKDGTASINFDDVVMRNMTVIKEGEVTFPPPPISVSAAPAKPAAKIAPKDTTPKAPSKLKY
VLAALGVAGFAAVASVAPPEFLSHFTVFLLSCVVGYYVVWNVSHSLHTPLMSVTNAISGIIVVGALLQIGQGSTLVTVLA
FIAVLIASINIFGGFTVTQRMLKMFRKD

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=539, Percent_Identity=48.9795918367347, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI122939153, Length=539, Percent_Identity=48.9795918367347, Blast_Score=463, Evalue=1e-130,
Organism=Escherichia coli, GI1787887, Length=512, Percent_Identity=73.4375, Blast_Score=749, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=520, Percent_Identity=50.5769230769231, Blast_Score=464, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 53478; Mature: 53478

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVVIEANAGQLSSFDDAAFEAAGAKVTTD
CCCCCCHHHHCCCCEEECCHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCEEEHH
VWQADLIFKVNAPSDAEIEQIKDGATVVSFIWPAQNPELVEKLSKRKINVMAMDMVPRIS
HCCEEEEEEECCCCCCHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEHHHHHHH
RAQSLDALSSMANIGGYRAVVEAAHQFGRFFTGQITAAGKVPPAKVLVIGAGVAGLAAIG
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEECEEEECCCCCCCEEEEEECCHHHHHHHC
TAGSLGAIVRAFDTRLEVAEQIESMGGQFLKLDFGNEEGGSSDGYAKTMSDEFIAAEMAL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHCCHHHHHHHHHH
FAEQAKEVDIIITTALIPGRPAPKLITKAMVDSMKSGSVIVDLAAATGGNCEYTQSGELF
HHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCEE
VTPNGVKVIGYTDLPGRLPAQSSQLYGTNLVNLMKLMCKEKDGTASINFDDVVMRNMTVI
ECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHCEEEE
KEGEVTFPPPPISVSAAPAKPAAKIAPKDTTPKAPSKLKYVLAALGVAGFAAVASVAPPE
ECCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
FLSHFTVFLLSCVVGYYVVWNVSHSLHTPLMSVTNAISGIIVVGALLQIGQGSTLVTVLA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FIAVLIASINIFGGFTVTQRMLKMFRKD
HHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVVIEANAGQLSSFDDAAFEAAGAKVTTD
CCCCCCHHHHCCCCEEECCHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCEEEHH
VWQADLIFKVNAPSDAEIEQIKDGATVVSFIWPAQNPELVEKLSKRKINVMAMDMVPRIS
HCCEEEEEEECCCCCCHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEHHHHHHH
RAQSLDALSSMANIGGYRAVVEAAHQFGRFFTGQITAAGKVPPAKVLVIGAGVAGLAAIG
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEECEEEECCCCCCCEEEEEECCHHHHHHHC
TAGSLGAIVRAFDTRLEVAEQIESMGGQFLKLDFGNEEGGSSDGYAKTMSDEFIAAEMAL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHCCHHHHHHHHHH
FAEQAKEVDIIITTALIPGRPAPKLITKAMVDSMKSGSVIVDLAAATGGNCEYTQSGELF
HHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCEE
VTPNGVKVIGYTDLPGRLPAQSSQLYGTNLVNLMKLMCKEKDGTASINFDDVVMRNMTVI
ECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHCEEEE
KEGEVTFPPPPISVSAAPAKPAAKIAPKDTTPKAPSKLKYVLAALGVAGFAAVASVAPPE
ECCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
FLSHFTVFLLSCVVGYYVVWNVSHSLHTPLMSVTNAISGIIVVGALLQIGQGSTLVTVLA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FIAVLIASINIFGGFTVTQRMLKMFRKD
HHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]