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The map label for this gene is eno [H]

Identifier: 126175328

GI number: 126175328

Start: 3661298

End: 3662593

Strand: Reverse

Name: eno [H]

Synonym: Sbal_3127

Alternate gene names: 126175328

Gene position: 3662593-3661298 (Counterclockwise)

Preceding gene: 126175329

Following gene: 126175327

Centisome position: 71.43

GC content: 46.53

Gene sequence:

>1296_bases
ATGGCTAAGATTATTAACGTGATTGGTCGCGAGATTATGGATTCTCGTGGTAACCCAACTGTTGAAGCCGAAGTGCATTT
AGAAGGTGGTTTTGTGGGTATGGCTGCGGCGCCTTCTGGCGCATCTACCGGTAGCCGCGAAGCGTTAGAATTACGTGATG
GCGATAAAAGCCGTTATTTAGGCAAAGGCGTATTAACGGCTGTTGCCAACGTAAACGATTTAATCCGCACTGCGTTATTA
GGCAAAGATGCCACTGCACAAGCTGAGCTTGATCAAATCATGATCGACTTAGACGGCACAGAAAACAAAGACAAGTTAGG
CGCAAACGCGATTCTGGCTGTGTCTTTAGCGGCTGCCAAAGCGGCTGCAGCATTTAAAGGTATTCCTTTATACGCGCACA
TCGCTGAATTGAACGGCACGCCAGGCCAATATTCTATGCCTGTGCCTATGATGAACATCTTAAACGGTGGCGAGCACGCG
GATAACAACGTTGATATCCAAGAATTCATGGTGCAACCAGTTGGCGCGAAAACTTTCCGTGAAGCACTGCGCATGGGCGC
TGAAATCTTCCATACGCTGAAAAAAGTATTGCACGACAAAGGTCTAAGCACTTCTGTTGGTGACGAAGGTGGTTTTGCAC
CTAACTTAGCGTCAAACGCTGATGCGTTAGCCATTATCAAAGAAGCGGTTGAACTGGCTGGCTACAAGTTAGGTACGGAT
GTGACCTTAGCGCTGGATTGCGCCGCATCTGAATTCTACAAAGACGGCAAATACGATTTAGCGGGCGAAGGCAAAGTATT
CGACTCGAACGGTTTCTCTGATTTCTTAAAATCATTGGCTGATCAATATCCAATCGTGTCTATCGAAGACGGCTTAGACG
AATCAGATTGGGATGGTTGGGCTTACCAAACTCAAATCATGGGCGACAAGATCCAATTAGTGGGCGACGATTTATTCGTT
ACTAACACTAAAATCTTAACCCGTGGTATCGAAAACGGTATTGCAAACTCAATCCTTATCAAGTTCAACCAAATCGGTTC
ATTGACTGAGACTTTAGCGGCCATTCGCATGGCGAAAGAAGCGGGTTACACAGCAGTGATTTCACACCGTAGTGGTGAAA
CTGAAGATTCAACGATTGCTGACTTAGCCGTAGGTACTGCTGCTGGCCAAATCAAAACCGGTTCACTGTGTCGTTCTGAC
CGTGTTGCTAAGTACAACCAATTGCTGCGCATCGAAGAGCAATTAGGTGAAAAAGCGCCATACCGTGGTTTGAAAGAGAT
CAAAGGTCAGGCGTAA

Upstream 100 bases:

>100_bases
CCGTAAATGGAGTTTTGTTATTTTCTGCGGTATGTGACTTCCATTTATTCCTATAGGTATTGGTTTTTTCTTTTATCTTT
AAACTTAAATCGAGGGTGTT

Downstream 100 bases:

>100_bases
TTTAAACTTTGATGTAAAAAGGCCACCACTTGGTGGCCTTTTTTGTTGTACTATCTGCTGTATATTTTTTTAATCCAAGA
TACTGACGCTAATTCCTCCA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAKIINVIGREIMDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRYLGKGVLTAVANVNDLIRTALL
GKDATAQAELDQIMIDLDGTENKDKLGANAILAVSLAAAKAAAAFKGIPLYAHIAELNGTPGQYSMPVPMMNILNGGEHA
DNNVDIQEFMVQPVGAKTFREALRMGAEIFHTLKKVLHDKGLSTSVGDEGGFAPNLASNADALAIIKEAVELAGYKLGTD
VTLALDCAASEFYKDGKYDLAGEGKVFDSNGFSDFLKSLADQYPIVSIEDGLDESDWDGWAYQTQIMGDKIQLVGDDLFV
TNTKILTRGIENGIANSILIKFNQIGSLTETLAAIRMAKEAGYTAVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSD
RVAKYNQLLRIEEQLGEKAPYRGLKEIKGQA

Sequences:

>Translated_431_residues
MAKIINVIGREIMDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRYLGKGVLTAVANVNDLIRTALL
GKDATAQAELDQIMIDLDGTENKDKLGANAILAVSLAAAKAAAAFKGIPLYAHIAELNGTPGQYSMPVPMMNILNGGEHA
DNNVDIQEFMVQPVGAKTFREALRMGAEIFHTLKKVLHDKGLSTSVGDEGGFAPNLASNADALAIIKEAVELAGYKLGTD
VTLALDCAASEFYKDGKYDLAGEGKVFDSNGFSDFLKSLADQYPIVSIEDGLDESDWDGWAYQTQIMGDKIQLVGDDLFV
TNTKILTRGIENGIANSILIKFNQIGSLTETLAAIRMAKEAGYTAVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSD
RVAKYNQLLRIEEQLGEKAPYRGLKEIKGQA
>Mature_430_residues
AKIINVIGREIMDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRYLGKGVLTAVANVNDLIRTALLG
KDATAQAELDQIMIDLDGTENKDKLGANAILAVSLAAAKAAAAFKGIPLYAHIAELNGTPGQYSMPVPMMNILNGGEHAD
NNVDIQEFMVQPVGAKTFREALRMGAEIFHTLKKVLHDKGLSTSVGDEGGFAPNLASNADALAIIKEAVELAGYKLGTDV
TLALDCAASEFYKDGKYDLAGEGKVFDSNGFSDFLKSLADQYPIVSIEDGLDESDWDGWAYQTQIMGDKIQLVGDDLFVT
NTKILTRGIENGIANSILIKFNQIGSLTETLAAIRMAKEAGYTAVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDR
VAKYNQLLRIEEQLGEKAPYRGLKEIKGQA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=428, Percent_Identity=53.2710280373832, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI301897469, Length=428, Percent_Identity=53.2710280373832, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=52.6806526806527, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=52.3255813953488, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI301897479, Length=426, Percent_Identity=48.3568075117371, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=27.6785714285714, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=27.6785714285714, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=27.6785714285714, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI1789141, Length=432, Percent_Identity=81.712962962963, Blast_Score=707, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=53.3642691415313, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=53.3642691415313, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI32563855, Length=208, Percent_Identity=49.0384615384615, Blast_Score=194, Evalue=9e-50,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=50.8120649651972, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.1916859122402, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.1916859122402, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.1916859122402, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=49.8839907192575, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580918, Length=427, Percent_Identity=51.0538641686183, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580916, Length=427, Percent_Identity=51.0538641686183, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580920, Length=427, Percent_Identity=51.0538641686183, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580914, Length=427, Percent_Identity=51.0538641686183, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI281360527, Length=424, Percent_Identity=51.4150943396226, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI17137654, Length=424, Percent_Identity=51.4150943396226, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45818; Mature: 45687

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIINVIGREIMDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRYL
CHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCEEEECCCCCHHHH
GKGVLTAVANVNDLIRTALLGKDATAQAELDQIMIDLDGTENKDKLGANAILAVSLAAAK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCEEEEECCCCCCCHHHCCCHHHHHHHHHHH
AAAAFKGIPLYAHIAELNGTPGQYSMPVPMMNILNGGEHADNNVDIQEFMVQPVGAKTFR
HHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRMGAEIFHTLKKVLHDKGLSTSVGDEGGFAPNLASNADALAIIKEAVELAGYKLGTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEECCCC
VTLALDCAASEFYKDGKYDLAGEGKVFDSNGFSDFLKSLADQYPIVSIEDGLDESDWDGW
EEEEEEHHHHHHHCCCCEEECCCCEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCE
AYQTQIMGDKIQLVGDDLFVTNTKILTRGIENGIANSILIKFNQIGSLTETLAAIRMAKE
EEEEEECCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEHHHCHHHHHHHHHHHHHH
AGYTAVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVAKYNQLLRIEEQLGEKAP
CCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
YRGLKEIKGQA
CCCHHHHCCCC
>Mature Secondary Structure 
AKIINVIGREIMDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRYL
HHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCEEEECCCCCHHHH
GKGVLTAVANVNDLIRTALLGKDATAQAELDQIMIDLDGTENKDKLGANAILAVSLAAAK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCEEEEECCCCCCCHHHCCCHHHHHHHHHHH
AAAAFKGIPLYAHIAELNGTPGQYSMPVPMMNILNGGEHADNNVDIQEFMVQPVGAKTFR
HHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRMGAEIFHTLKKVLHDKGLSTSVGDEGGFAPNLASNADALAIIKEAVELAGYKLGTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEECCCC
VTLALDCAASEFYKDGKYDLAGEGKVFDSNGFSDFLKSLADQYPIVSIEDGLDESDWDGW
EEEEEEHHHHHHHCCCCEEECCCCEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCE
AYQTQIMGDKIQLVGDDLFVTNTKILTRGIENGIANSILIKFNQIGSLTETLAAIRMAKE
EEEEEECCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEHHHCHHHHHHHHHHHHHH
AGYTAVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVAKYNQLLRIEEQLGEKAP
CCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
YRGLKEIKGQA
CCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA