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The map label for this gene is xapA [H]

Identifier: 126175276

GI number: 126175276

Start: 3596525

End: 3597352

Strand: Reverse

Name: xapA [H]

Synonym: Sbal_3075

Alternate gene names: 126175276

Gene position: 3597352-3596525 (Counterclockwise)

Preceding gene: 126175282

Following gene: 126175274

Centisome position: 70.16

GC content: 50.24

Gene sequence:

>828_bases
ATGAGCCATGATCCTGTTATTAATGCCCTTGCGACGATTCGAGCCCGTAAACCGAATTTCTCTCCCCGCGCCGCGATGAT
TTTGGGCTCAGGGCTTGGCGCGTTTGCCGATAATTTAGATAACAAAGTAGTGATCCCCTATGAAGAGTTAGATGGCTTTC
CCGTGAGTACCGTCGTCGGTCACTCGGGAGAGCTGGTGCTCGGCAGCTTACATGGCATCGATATAGTGTGCATGAAAGGC
CGTGGGCATTTTTACGAGCACCAAAGCATGAAGGTGATGACGACGCCGGTACGTACTTTCAAACGTTTAGGGTGTGAATT
GTTACTCGTGACTAATGCTGCGGGCTCATTACGACCAGAGCGGATCGGCGTGGGTTCATTAGTGATTTTCAGCGATCACA
TCAACACTATGCCGGGTACGCCTATGACGGGCGCAAACGATGATAGTTACGGTCCACGTTTTTTTAGCCTAGCCAATGCT
TATGATAAAGACCTCAGAGCAGAAGCCTTAAGTGTCGCAAAAGCGGCTGGGATTACAGTGAATCAAGGGGTCTTTGTGTC
TTACTCTGGCCCGTGTTTTGAAACCGCGGCTGAAATCCGCATGATGCAAATTATCGGTGGCGATGTGGTGGGAATGTCTG
TGGTGCCAGAGGTGATTTCGGCGGCGCATTGTGGTTTGCCCGTCTTGGCTGTGTGTGCCATTACCAATATGGCCGAAGGA
TTGGGGGATGTGCAGTTATCCCATGAACAAACCTTAACATCCGCCAAGCTTGCCGAAGCCGACTTTATCCGTTTGATTGA
GCGTTTTACCGCCAGTCATTTTGCTTAA

Upstream 100 bases:

>100_bases
TCAGCGCAATAGTGCGGTTAATCGCGATTATTTGCAACCCCAAGCCCGCTTATACTGAGGGTCGTTTACTCTTTCATTCC
TTTGCTATGAGGTACCTTGT

Downstream 100 bases:

>100_bases
CATCTGGTTTAACTGGCTGCTATATAGGGTAGTGCTGCGATAAAGGGCTGCTATTTTAAAAGCTCGTATTTAACAGGTCG
AATTAAGATCTCGTATTTAA

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSHDPVINALATIRARKPNFSPRAAMILGSGLGAFADNLDNKVVIPYEELDGFPVSTVVGHSGELVLGSLHGIDIVCMKG
RGHFYEHQSMKVMTTPVRTFKRLGCELLLVTNAAGSLRPERIGVGSLVIFSDHINTMPGTPMTGANDDSYGPRFFSLANA
YDKDLRAEALSVAKAAGITVNQGVFVSYSGPCFETAAEIRMMQIIGGDVVGMSVVPEVISAAHCGLPVLAVCAITNMAEG
LGDVQLSHEQTLTSAKLAEADFIRLIERFTASHFA

Sequences:

>Translated_275_residues
MSHDPVINALATIRARKPNFSPRAAMILGSGLGAFADNLDNKVVIPYEELDGFPVSTVVGHSGELVLGSLHGIDIVCMKG
RGHFYEHQSMKVMTTPVRTFKRLGCELLLVTNAAGSLRPERIGVGSLVIFSDHINTMPGTPMTGANDDSYGPRFFSLANA
YDKDLRAEALSVAKAAGITVNQGVFVSYSGPCFETAAEIRMMQIIGGDVVGMSVVPEVISAAHCGLPVLAVCAITNMAEG
LGDVQLSHEQTLTSAKLAEADFIRLIERFTASHFA
>Mature_274_residues
SHDPVINALATIRARKPNFSPRAAMILGSGLGAFADNLDNKVVIPYEELDGFPVSTVVGHSGELVLGSLHGIDIVCMKGR
GHFYEHQSMKVMTTPVRTFKRLGCELLLVTNAAGSLRPERIGVGSLVIFSDHINTMPGTPMTGANDDSYGPRFFSLANAY
DKDLRAEALSVAKAAGITVNQGVFVSYSGPCFETAAEIRMMQIIGGDVVGMSVVPEVISAAHCGLPVLAVCAITNMAEGL
GDVQLSHEQTLTSAKLAEADFIRLIERFTASHFA

Specific function: The nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the nucleoside molecule, with the formation of the corresponding free bases and pentose-1-phosphate. This protein can degrade all purine nucleosides except ade

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=261, Percent_Identity=42.911877394636, Blast_Score=194, Evalue=5e-50,
Organism=Homo sapiens, GI47132622, Length=197, Percent_Identity=28.4263959390863, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788746, Length=269, Percent_Identity=67.2862453531598, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17541190, Length=266, Percent_Identity=37.593984962406, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6323238, Length=273, Percent_Identity=40.2930402930403, Blast_Score=170, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24656090, Length=273, Percent_Identity=39.1941391941392, Blast_Score=197, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24656093, Length=273, Percent_Identity=39.1941391941392, Blast_Score=197, Evalue=5e-51,
Organism=Drosophila melanogaster, GI45552887, Length=273, Percent_Identity=39.1941391941392, Blast_Score=197, Evalue=6e-51,
Organism=Drosophila melanogaster, GI45552885, Length=273, Percent_Identity=39.1941391941392, Blast_Score=197, Evalue=6e-51,
Organism=Drosophila melanogaster, GI24762376, Length=263, Percent_Identity=32.6996197718631, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI20130079, Length=225, Percent_Identity=27.5555555555556, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221459247, Length=207, Percent_Identity=27.0531400966184, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR010943
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: 2.4.2.-

Molecular weight: Translated: 29265; Mature: 29134

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01240 PNP_MTAP_2 ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDPVINALATIRARKPNFSPRAAMILGSGLGAFADNLDNKVVIPYEELDGFPVSTVVG
CCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCCCEEEEEHHHCCCCCCHHEEC
HSGELVLGSLHGIDIVCMKGRGHFYEHQSMKVMTTPVRTFKRLGCELLLVTNAAGSLRPE
CCCCEEEEEECCEEEEEECCCCCEEECCCCEEEEHHHHHHHHCCCEEEEEECCCCCCCCC
RIGVGSLVIFSDHINTMPGTPMTGANDDSYGPRFFSLANAYDKDLRAEALSVAKAAGITV
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
NQGVFVSYSGPCFETAAEIRMMQIIGGDVVGMSVVPEVISAAHCGLPVLAVCAITNMAEG
CCCEEEEECCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
LGDVQLSHEQTLTSAKLAEADFIRLIERFTASHFA
CCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHDPVINALATIRARKPNFSPRAAMILGSGLGAFADNLDNKVVIPYEELDGFPVSTVVG
CCCHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCCCEEEEEHHHCCCCCCHHEEC
HSGELVLGSLHGIDIVCMKGRGHFYEHQSMKVMTTPVRTFKRLGCELLLVTNAAGSLRPE
CCCCEEEEEECCEEEEEECCCCCEEECCCCEEEEHHHHHHHHCCCEEEEEECCCCCCCCC
RIGVGSLVIFSDHINTMPGTPMTGANDDSYGPRFFSLANAYDKDLRAEALSVAKAAGITV
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
NQGVFVSYSGPCFETAAEIRMMQIIGGDVVGMSVVPEVISAAHCGLPVLAVCAITNMAEG
CCCEEEEECCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
LGDVQLSHEQTLTSAKLAEADFIRLIERFTASHFA
CCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559336; 9205837; 9278503 [H]