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The map label for this gene is phrA [H]

Identifier: 126175256

GI number: 126175256

Start: 3575430

End: 3576947

Strand: Reverse

Name: phrA [H]

Synonym: Sbal_3054

Alternate gene names: 126175256

Gene position: 3576947-3575430 (Counterclockwise)

Preceding gene: 126175257

Following gene: 126175255

Centisome position: 69.76

GC content: 48.88

Gene sequence:

>1518_bases
ATGACATCAGTTAACGATTCAATGGCAATAGATTCAATGCCAATAGATTCAATGTCAATAAAAGTGCAGGGCAATAATGC
ATTGATGTGGTTTCGCCAAGATTTACGCCTTGCCGATAATCAGGCATTAACCGCTGCTTGCGATTGGGCGCGGGCGAATG
GCGTGGCACTTAAGGCGATTTATATCGCTACGCCAACCCAATGGCAACGCCATGATGTGGCGCCCATTCAGTTGGATTTT
ATCGAACGTCACATCAATCTGCTTGCCCAAGGGCTGGCGAGTTTGGGTATTACATTCGAATTAATTCAGTTAGATGCCTT
TGCCGATGTGCCAGCGTTTATCGGTCATTATTGTCAGCAGCAGGGTATTAGCCGCGTGTTTGCCGGTAGTGAGCCTGAGA
TTAACGAGCAACAGCGTGACCAAGCCTGTATTCAGGCGGGTATTCCTCTGGTGCTAACCGATGAGCATTGTTTGCTTAAG
CCGGGCACAGTGCTGAATTTGTCCGGCGACATGTATAAGGTGTTCACCCCTTTTAGCCGTAAGTGGCGAGAAATTGCCGC
CCGTCATGCCATTTTTCTGCTTCCAGTTCCGGCGCCGCTTGGACCTGTGTTGACCGAGCCAAAGCCGCATTTGTTTAACA
ATGTCACTAAAGTATCGAGTGAACTTTGGGCTGCGGGAGAAGGGCAGGCAAAGCGTATACTTTCAGCATTTATTCAGCAG
AAAGTGCAGGATTATAAGCAAGATAGGGATTTCCCCGCCATCGATGGCACCAGTTCAATTTCACCTTATCTGGCCATTGG
CGTCATTAGCCCAAGACAATGCGTTGCCGCGTTGCTGCATGACTTCCCCGAGGTGATTGTCGACGATACTAGTCCAGCCC
GTACTTGGTTAAATGAGCTTACCTGGCGTGAGTTTTATCGTCATTTGCTGGTGGCATTTCCTGATTTATCTAAGAATCAT
AATTTCAATCGACAAGCCGACCATGTGCAGTGGCGAAACAATCCGCAGGAGTTTGCTGCTTGGTGTGAAGGTCGTACTGG
CTATCCGATTGTCGATGCGGCTATGCGCCAACTCAATCAAACGGGTTGGATGCACAACCGTTTGCGTATGGTGGTGGCGA
GTTTCCTGACTAAACATCTACTGATTGATTGGCGTTGGGGCGAGCGTTATTTCCGCCAAAAGCTCATCGATGGTGACTTG
GCTGCAAATAATGGCGGCTGGCAGTGGTCCGCCGGTTGTGGCTGCGATGCCCAGCCTTATTTCCGCATTTTTAATCCTAT
GAGCCAGAGTGAAAAGTTTGATCCAGATGGCAGCTTCATCCGTAAGTACTTACCAGAGCTCGCATCTTGGGGAATTAAGC
AATTACATCAACCCAGTACAGCGCAGACACCGTCCTTGTTTGAACAGCCGTTATTTAACATTAAAACGGCTTATCCGAGT
GCGATTGTCGAGCATAGTGCAGCAAGAGCAAGGGCGTTAACCGTACTAGGCGTATTGAAAAAGGCCGCGGCCCAATAA

Upstream 100 bases:

>100_bases
ATACCGTCTCGCTCCATGGGTAAGGTCAACATAAGTCACAAAGGCAAAGGGAAAAGTATTGGCGTGAGTCAAAGCGAGCA
GGGCAATAGTGGAGAAAACA

Downstream 100 bases:

>100_bases
ATAGGATCAGGAGCAATAACCAGATGACAAACCAAGCGACGACTGAGACTAATACGCTTATTGATGATTTTATTCAGCTT
TATCAAGCCTTGAATAAAGA

Product: deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MTSVNDSMAIDSMPIDSMSIKVQGNNALMWFRQDLRLADNQALTAACDWARANGVALKAIYIATPTQWQRHDVAPIQLDF
IERHINLLAQGLASLGITFELIQLDAFADVPAFIGHYCQQQGISRVFAGSEPEINEQQRDQACIQAGIPLVLTDEHCLLK
PGTVLNLSGDMYKVFTPFSRKWREIAARHAIFLLPVPAPLGPVLTEPKPHLFNNVTKVSSELWAAGEGQAKRILSAFIQQ
KVQDYKQDRDFPAIDGTSSISPYLAIGVISPRQCVAALLHDFPEVIVDDTSPARTWLNELTWREFYRHLLVAFPDLSKNH
NFNRQADHVQWRNNPQEFAAWCEGRTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQKLIDGDL
AANNGGWQWSAGCGCDAQPYFRIFNPMSQSEKFDPDGSFIRKYLPELASWGIKQLHQPSTAQTPSLFEQPLFNIKTAYPS
AIVEHSAARARALTVLGVLKKAAAQ

Sequences:

>Translated_505_residues
MTSVNDSMAIDSMPIDSMSIKVQGNNALMWFRQDLRLADNQALTAACDWARANGVALKAIYIATPTQWQRHDVAPIQLDF
IERHINLLAQGLASLGITFELIQLDAFADVPAFIGHYCQQQGISRVFAGSEPEINEQQRDQACIQAGIPLVLTDEHCLLK
PGTVLNLSGDMYKVFTPFSRKWREIAARHAIFLLPVPAPLGPVLTEPKPHLFNNVTKVSSELWAAGEGQAKRILSAFIQQ
KVQDYKQDRDFPAIDGTSSISPYLAIGVISPRQCVAALLHDFPEVIVDDTSPARTWLNELTWREFYRHLLVAFPDLSKNH
NFNRQADHVQWRNNPQEFAAWCEGRTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQKLIDGDL
AANNGGWQWSAGCGCDAQPYFRIFNPMSQSEKFDPDGSFIRKYLPELASWGIKQLHQPSTAQTPSLFEQPLFNIKTAYPS
AIVEHSAARARALTVLGVLKKAAAQ
>Mature_504_residues
TSVNDSMAIDSMPIDSMSIKVQGNNALMWFRQDLRLADNQALTAACDWARANGVALKAIYIATPTQWQRHDVAPIQLDFI
ERHINLLAQGLASLGITFELIQLDAFADVPAFIGHYCQQQGISRVFAGSEPEINEQQRDQACIQAGIPLVLTDEHCLLKP
GTVLNLSGDMYKVFTPFSRKWREIAARHAIFLLPVPAPLGPVLTEPKPHLFNNVTKVSSELWAAGEGQAKRILSAFIQQK
VQDYKQDRDFPAIDGTSSISPYLAIGVISPRQCVAALLHDFPEVIVDDTSPARTWLNELTWREFYRHLLVAFPDLSKNHN
FNRQADHVQWRNNPQEFAAWCEGRTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQKLIDGDLA
ANNGGWQWSAGCGCDAQPYFRIFNPMSQSEKFDPDGSFIRKYLPELASWGIKQLHQPSTAQTPSLFEQPLFNIKTAYPSA
IVEHSAARARALTVLGVLKKAAAQ

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=492, Percent_Identity=26.0162601626016, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI4758072, Length=491, Percent_Identity=26.2729124236253, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI188536103, Length=399, Percent_Identity=26.3157894736842, Blast_Score=142, Evalue=8e-34,
Organism=Escherichia coli, GI1786926, Length=480, Percent_Identity=45, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324962, Length=497, Percent_Identity=31.9919517102616, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17137248, Length=512, Percent_Identity=26.5625, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24585455, Length=512, Percent_Identity=26.5625, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24648152, Length=530, Percent_Identity=26.2264150943396, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 56864; Mature: 56733

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVNDSMAIDSMPIDSMSIKVQGNNALMWFRQDLRLADNQALTAACDWARANGVALKAI
CCCCCCCCEECCCCCCCEEEEEECCCEEEEEHHHHHHCCCCHHHHHHHHHCCCCEEEEEE
YIATPTQWQRHDVAPIQLDFIERHINLLAQGLASLGITFELIQLDAFADVPAFIGHYCQQ
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHCCHHHHHHHHHH
QGISRVFAGSEPEINEQQRDQACIQAGIPLVLTDEHCLLKPGTVLNLSGDMYKVFTPFSR
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCEEECCCCEEECCCCEEEECCHHHH
KWREIAARHAIFLLPVPAPLGPVLTEPKPHLFNNVTKVSSELWAAGEGQAKRILSAFIQQ
HHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
KVQDYKQDRDFPAIDGTSSISPYLAIGVISPRQCVAALLHDFPEVIVDDTSPARTWLNEL
HHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCHHHEECCCCHHHHHHHHH
TWREFYRHLLVAFPDLSKNHNFNRQADHVQWRNNPQEFAAWCEGRTGYPIVDAAMRQLNQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
TGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQKLIDGDLAANNGGWQWSAGCGCDAQPY
HCHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCH
FRIFNPMSQSEKFDPDGSFIRKYLPELASWGIKQLHQPSTAQTPSLFEQPLFNIKTAYPS
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHH
AIVEHSAARARALTVLGVLKKAAAQ
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSVNDSMAIDSMPIDSMSIKVQGNNALMWFRQDLRLADNQALTAACDWARANGVALKAI
CCCCCCCEECCCCCCCEEEEEECCCEEEEEHHHHHHCCCCHHHHHHHHHCCCCEEEEEE
YIATPTQWQRHDVAPIQLDFIERHINLLAQGLASLGITFELIQLDAFADVPAFIGHYCQQ
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHCCHHHHHHHHHH
QGISRVFAGSEPEINEQQRDQACIQAGIPLVLTDEHCLLKPGTVLNLSGDMYKVFTPFSR
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCEEECCCCEEECCCCEEEECCHHHH
KWREIAARHAIFLLPVPAPLGPVLTEPKPHLFNNVTKVSSELWAAGEGQAKRILSAFIQQ
HHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
KVQDYKQDRDFPAIDGTSSISPYLAIGVISPRQCVAALLHDFPEVIVDDTSPARTWLNEL
HHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCHHHEECCCCHHHHHHHHH
TWREFYRHLLVAFPDLSKNHNFNRQADHVQWRNNPQEFAAWCEGRTGYPIVDAAMRQLNQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
TGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQKLIDGDLAANNGGWQWSAGCGCDAQPY
HCHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCH
FRIFNPMSQSEKFDPDGSFIRKYLPELASWGIKQLHQPSTAQTPSLFEQPLFNIKTAYPS
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHH
AIVEHSAARARALTVLGVLKKAAAQ
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]