Click here to switch to the map view.

The map label for this gene is trmB [H]

Identifier: 126175240

GI number: 126175240

Start: 3562311

End: 3563027

Strand: Reverse

Name: trmB [H]

Synonym: Sbal_3038

Alternate gene names: 126175240

Gene position: 3563027-3562311 (Counterclockwise)

Preceding gene: 126175243

Following gene: 126175239

Centisome position: 69.49

GC content: 48.26

Gene sequence:

>717_bases
ATGAGCGAAGTCACTACCGCTGAATTTAATGAAGAAGGCAAGTATCTGCGTAAGATCAGAAGCTTTGTCCTAAGAGAAGG
TCGCTTAACTAAAGGCCAAGCCCAAGCTATTGAAAGCCAGTGGCCAACAATGGGGTTAGATTACAGCCCAACGCCATTGG
TTCTGAGTGACGTTTTTGGCCGTGAAGCCGATACTGTGCTGGAAATTGGCTTTGGCATGGGCGCCTCTTTAGTGCAAATG
GCTAAAGACGCACCAGAGCAAAATTTTATTGGTATTGAAGTGCATAAACCGGGTGTCGGTTCTTGCTTAAGCGATGCCGC
CATCGCGGGTGTGACTAATTTACGTGTGTATCATCATGATGCCATGGAAGTATTAGAACATGCGATTGCCGACGGTTCAT
TGGCCCGAGTGCAATTGTTTTTCCCCGATCCTTGGCATAAGAAACGTCATCATAAGCGTCGTATCGTACAAGCTGAATTT
GCTGAACTGATCCGCCGCAAGCTAAAAATTGGCGGCGTGTTCCATATGGCAACTGATTGGGAAGAATACAGCGAGCACAT
GTTGGAAGTGATGCAAGCGGCTCCTGGTTATCAAAACCAGTCGAGTGACGGCACTGTGGTGCCACGTCCTGATCATCGCC
CACTGACAAAGTTTGAAGCCCGCGGCCATAGATTGGGTCACGGTGTATGGGATCTGATGTTTGAGCGTATCGCTTAA

Upstream 100 bases:

>100_bases
AATCTTGGTATACTTTCGCCCCATTAGTGCTTGCACTGTATGTTGAACTTTGGATAATGCCCTTCTTTTATGATGGTGCT
GTCCACTAGACGAGGCAAAA

Downstream 100 bases:

>100_bases
TCTAGATATTTCAATTGATTAATTAGTTTTTTAACACAACGGGGAAAGTCACTATGGCAACTAATCGTAGCCGTCGTTTA
CGTAAGAAAATGCGCGTTGA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPTPLVLSDVFGREADTVLEIGFGMGASLVQM
AKDAPEQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHDAMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEF
AELIRRKLKIGGVFHMATDWEEYSEHMLEVMQAAPGYQNQSSDGTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIA

Sequences:

>Translated_238_residues
MSEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPTPLVLSDVFGREADTVLEIGFGMGASLVQM
AKDAPEQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHDAMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEF
AELIRRKLKIGGVFHMATDWEEYSEHMLEVMQAAPGYQNQSSDGTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIA
>Mature_237_residues
SEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPTPLVLSDVFGREADTVLEIGFGMGASLVQMA
KDAPEQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHDAMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEFA
ELIRRKLKIGGVFHMATDWEEYSEHMLEVMQAAPGYQNQSSDGTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=236, Percent_Identity=65.2542372881356, Blast_Score=327, Evalue=5e-91,
Organism=Saccharomyces cerevisiae, GI6320000, Length=148, Percent_Identity=28.3783783783784, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 26831; Mature: 26700

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPTPLVLSDVFG
CCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHC
READTVLEIGFGMGASLVQMAKDAPEQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHD
CCHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHEEEEHHH
AMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEFAELIRRKLKIGGVFHMATDW
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCH
EEYSEHMLEVMQAAPGYQNQSSDGTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIA
HHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPTPLVLSDVFG
CCCCCCHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHC
READTVLEIGFGMGASLVQMAKDAPEQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHD
CCHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHEEEEHHH
AMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEFAELIRRKLKIGGVFHMATDW
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCH
EEYSEHMLEVMQAAPGYQNQSSDGTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIA
HHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA