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The map label for this gene is pilT [H]

Identifier: 126175226

GI number: 126175226

Start: 3548696

End: 3549733

Strand: Reverse

Name: pilT [H]

Synonym: Sbal_3024

Alternate gene names: 126175226

Gene position: 3549733-3548696 (Counterclockwise)

Preceding gene: 126175236

Following gene: 126175225

Centisome position: 69.23

GC content: 48.07

Gene sequence:

>1038_bases
ATGGAAATCACTGAGTTATTGGCATTTAGTGTAAAACACAAAGCCTCGGATCTACACCTTTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAGATTAACCTGCCCGCGCTCGATCACCAAGGCGTACACAGCCTAGTGTACGACA
TAATGAATGATAAGCAGCGCAAGGACTACGAAGAACATTTAGAAATCGATTTCTCGTTCGAAGTCCCTAATCTTGCACGC
TTTCGTGTTAACGCATTCAATCAATCCCGTGGTGCCGCGGCCGTATTTCGTACCATTCCTAGCGACATCTTGTCACTTGA
GCAATTGGGTGCGCCGGAGATTTTTAAAAAAATCGCCAGTTTCCCTCGGGGATTAGTCCTAGTGACTGGGCCAACGGGTT
CGGGTAAGAGTACCACACTTGCCGCTATGGTGGATTACGTTAACGAAAATCGACACGATCACATCTTAACCATTGAAGAT
CCTATCGAATTCGTACACCAAAATAAGCAATGTTTGATTAACCAGCGTGAAGTTCACCGCCATACCCACAGCTTTAATGC
GGCGCTTAGAAGCGCACTGCGTGAAGATCCGGACGTGATTTTAGTCGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CGATGACCGCTGCAGAAACGGGCCACTTAGTGTTTGGTACCTTGCATACCACCTCCGCCGCGAAAACCATTGACCGTGTG
GTCGACGTATTCCCCGCCGGTGAAAAAGACATGGTGCGCACTATGTTGTCGGAATCACTGCAAGCGGTTATTTCACAAAC
CCTGATTAAAAAGGTAGGCGGAGGCCGTGTCGCCGCCCATGAAATCATGATGGGCACTCCCGCTATTCGTAACCTTATTC
GTGAAGACAAAGTGGCGCAGATGTATTCGGCGATTCAAACCAGTATGGCCCACGGAATGCAGACCTTAGAGCAATGTTTA
CAGAACTTAGTCAATCGCGGTCTTATCACCCGTGAAGATGCAATGGCGAAGAGTTCGAATAAACAAGCGACATTTTAA

Upstream 100 bases:

>100_bases
CATTTTTGCGCCGCTTGTGCGATCCTGCGCTGGGCGATTGCGAGTCTGTCTGCTATTGTTGTCATGATGTAATTATCTAG
TTGCAAGGATATCCCAAACG

Downstream 100 bases:

>100_bases
GGATTTATGATGGATGTTCTTCCGTTTTTAAAAGTGATCGTTGAGCGCAAAGCCTCCGACTTGTTCATCACAGCGGGCTT
TCCGCCAAGTGCCAAGATTG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTSMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Sequences:

>Translated_345_residues
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTSMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF
>Mature_345_residues
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTSMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=48.0122324159021, Blast_Score=300, Evalue=6e-83,
Organism=Escherichia coli, GI1789723, Length=356, Percent_Identity=32.0224719101124, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786296, Length=283, Percent_Identity=33.2155477031802, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38329; Mature: 38329

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQR
CCHHHHHHHHHHHCCCCEEEECCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHCCHHH
KDYEEHLEIDFSFEVPNLARFRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIAS
CCHHHHCEEEEEECCCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIEDPIEFVHQNKQCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHHCCHHHHHH
HTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHH
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
MYSAIQTSMAHGMQTLEQCLQNLVNRGLITREDAMAKSSNKQATF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQR
CCHHHHHHHHHHHCCCCEEEECCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHCCHHH
KDYEEHLEIDFSFEVPNLARFRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIAS
CCHHHHCEEEEEECCCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIEDPIEFVHQNKQCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHHCCHHHHHH
HTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHH
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
MYSAIQTSMAHGMQTLEQCLQNLVNRGLITREDAMAKSSNKQATF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]