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The map label for this gene is pilT [H]

Identifier: 126175225

GI number: 126175225

Start: 3547574

End: 3548686

Strand: Reverse

Name: pilT [H]

Synonym: Sbal_3023

Alternate gene names: 126175225

Gene position: 3548686-3547574 (Counterclockwise)

Preceding gene: 126175226

Following gene: 126175224

Centisome position: 69.21

GC content: 47.89

Gene sequence:

>1113_bases
ATGGATGTTCTTCCGTTTTTAAAAGTGATCGTTGAGCGCAAAGCCTCCGACTTGTTCATCACAGCGGGCTTTCCGCCAAG
TGCCAAGATTGACGGTGAGTTGCGTCCCTTGGCCGAAAGTGCCTTTACTCCGGCACAATCAATGGATTTTGTTGAATCCT
TAATGACCGAAGCGCAGAAACAAGAGTTTCATTCAACTCGTGAGTGTAACTTTGCTTTTGCTGCAAAGGATTTAGGTCGT
TTTCGGGTGAGCGCCTTTTGGCAGCGTGAATCGCCAGGCTGCGTGATGCGTCGTATCGAAACTAAAATCCCTGACGTCGA
TGATTTAAAACTGCCACCCATTCTGAAAGATCTGGTGATGAGTAAACGTGGTCTCATTATTATGGTGGGCGGAACGGGTA
CGGGTAAGTCAACATCTCTGGCGGCCTTGGTGGGATATCGTAATGGTCATGCTCGTGGTCATATTCTCACGATTGAAGAT
CCAGTCGAATTTGTTCACGACCACAGAAAAAGCATTATCACTCAGCGTGAAGTGGGCATAGATACTGAGTCTTTCGATGC
GGCGCTAAAAAGCTCCCTTCGCCAAGCACCCGATGTGATCTTGATTGGTGAAATTCGTACCCAAGAGACGATGGAGTTCG
CCCTGTCGTTTGCGGAAACAGGCCACCTCTGTATGGCAACCTTGCATGCCAACAACGCCAACCAAGCATTAGACCGCATT
ATGCACCTCGTGCCTGAGAGTAAACATCAACAGTTATTGTTCGATTTGTCATTGAATTTACGCGGGATTGTGGCGCAGCA
ATTGATCCCAAAAGTCGATGGCACTGGGCGTCGCGCCGCGATTGAAGTATTGATTAACACCCCAAGGGTGGCGAGTTTAA
TTGCGAAGAACGAGCTGCATTTGCTCAAAGAAACCATGGCCAAATCGAATGAGCAGGGCATGCAGACGTTCGATCAAGCC
TTGCTCAAACTTTATGTCGATGGCGAGATTAGTTACGCCGATGCGCTGCACCATGCTGATTCACCGAACGATTTACGTTT
GATGATTAAGTTGCAGAATAAAGAATCCACGGGCTCAGGCTTTATGGAAGGTGTCACCTTAGACATGGATTAA

Upstream 100 bases:

>100_bases
AGAGCAATGTTTACAGAACTTAGTCAATCGCGGTCTTATCACCCGTGAAGATGCAATGGCGAAGAGTTCGAATAAACAAG
CGACATTTTAAGGATTTATG

Downstream 100 bases:

>100_bases
CGCGAGAGCCAGGTTCTAGGTTAAGCAGCTCTTTATGGGCTGCTTTTTGTTAGTGTCGTTAGATTTTCGTTCAGCTCAAT
CGGTTTTACTTCATCGCTAT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSMDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Sequences:

>Translated_370_residues
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSMDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD
>Mature_370_residues
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSMDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=321, Percent_Identity=36.1370716510903, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789723, Length=159, Percent_Identity=40.251572327044, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786296, Length=282, Percent_Identity=28.7234042553192, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41005; Mature: 41005

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSMDFVESLMTEAQK
CCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
QEFHSTRECNFAFAAKDLGRFRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVM
HHHHCHHCCCEEEHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHH
SKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIEDPVEFVHDHRKSIITQREVGI
CCCCEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC
DTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELH
HHHCCCCCHHEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
LLKETMAKSNEQGMQTFDQALLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSG
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCC
FMEGVTLDMD
CCCCEEEECC
>Mature Secondary Structure
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSMDFVESLMTEAQK
CCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
QEFHSTRECNFAFAAKDLGRFRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVM
HHHHCHHCCCEEEHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHH
SKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIEDPVEFVHDHRKSIITQREVGI
CCCCEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC
DTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELH
HHHCCCCCHHEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
LLKETMAKSNEQGMQTFDQALLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSG
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCC
FMEGVTLDMD
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]