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The map label for this gene is rlmN [H]

Identifier: 126175195

GI number: 126175195

Start: 3520261

End: 3521382

Strand: Reverse

Name: rlmN [H]

Synonym: Sbal_2993

Alternate gene names: 126175195

Gene position: 3521382-3520261 (Counterclockwise)

Preceding gene: 126175198

Following gene: 126175194

Centisome position: 68.68

GC content: 45.9

Gene sequence:

>1122_bases
ATGAGTGAAAAGAAGATCAATTTATTAGACCTTGATCGTAAGGCGATGCGCGCATTATTTGCGGACATGGGAGAGAAACC
CTTTCGTGCCGATCAGCTCATGAAATGGCTATATCATTTCGGTGTCAGTGACTTCGAAGAGATGACCAACATCAACAAAG
TGTTACGTCAAAAATTGGCGGCACGCTGCGAGATCGTTGCGCCGGAAATTTCGAGCTTCCAAAAATCGACCGATGGGACG
ATTAAATTCGCGATCAACGTTGGTCAAGGCCAAGAAGTTGAAACGGTTTATATTCCTGAGGATGACCGCGCGACTTTGTG
TGTCTCATCCCAAGTGGGTTGTGCGCTGGAATGTACTTTCTGCTCTACGGGTCAGCAAGGTTTTAACCGTAACTTAACGG
TTTCTGAAATCGTCGGCCAAATTTGGCGTGTATCGCACTTTTTAGGTTTTGCTAAAGACACGGGCGAGCGTCCGATCACT
AACGTGGTGATGATGGGTATGGGTGAGCCATTGCTCAACCTAGCCAATGTCATACCAGCAATGGACATCATGCTCGATGA
TTTTGGTTTTAGCTTGTCAAAACGCCGCGTGACCTTATCAACCTCAGGCGTAGTGCCTGCGTTAGATAAACTGGGCGATG
CCATTGATGTGGCACTTGCCGTCAGTATCCATGCGCCAAATGATGAGCTGCGTGACATTCTGGTCCCAATCAATAAAAAA
TATCAGCTGGATGAGTTTTTAGCGGGTATTCGTCGTTATATCGCTAAATCTAATGCAAACCGTGGCCGTGTGACGGTTGA
ATACGTGATGCTAGATCACATCAACGACAGCACAGATCAAGCCCATGAGTTAGCTAAGTTGATGAAAGATACACCTTGTA
AGATAAATCTGATCCCATTTAACCCGTATCCAGGCTCGCCTTACGGCCGTTCATCTAACTCTCGTATCGACCGTTTTTCT
AAGGTGCTTATGGAGTACGGTTTTACTGTGATCGTGCGTAAAACCCGTGGCGATGATATTGATGCCGCCTGTGGTCAGTT
AGCTGGGGATATTCGCGATAGAACGAAACGTTTAGCAAAAAAACGCATGCAAGAAAACCAGATTTCAGTCACAATGAATT
AA

Upstream 100 bases:

>100_bases
TTCTAGCATAGGTTAATTGAGTTACTGGCTAAAATTTATTTGCGTCTGTATAATTCGCCGCCCTGTTACAGGATCCGGTT
TTGGTGTTGAGGTTAGCTAT

Downstream 100 bases:

>100_bases
CTCTTTGTTTGCACAGATTTATAGGCTTGCCCATGAAGCATGGATTGCCAAGGTTTTCGTTGATTTTAACCCTGTCATTA
TTGATGACAGGGTGCGTCAC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSEKKINLLDLDRKAMRALFADMGEKPFRADQLMKWLYHFGVSDFEEMTNINKVLRQKLAARCEIVAPEISSFQKSTDGT
IKFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTGQQGFNRNLTVSEIVGQIWRVSHFLGFAKDTGERPIT
NVVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDAIDVALAVSIHAPNDELRDILVPINKK
YQLDEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKINLIPFNPYPGSPYGRSSNSRIDRFS
KVLMEYGFTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN

Sequences:

>Translated_373_residues
MSEKKINLLDLDRKAMRALFADMGEKPFRADQLMKWLYHFGVSDFEEMTNINKVLRQKLAARCEIVAPEISSFQKSTDGT
IKFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTGQQGFNRNLTVSEIVGQIWRVSHFLGFAKDTGERPIT
NVVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDAIDVALAVSIHAPNDELRDILVPINKK
YQLDEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKINLIPFNPYPGSPYGRSSNSRIDRFS
KVLMEYGFTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN
>Mature_372_residues
SEKKINLLDLDRKAMRALFADMGEKPFRADQLMKWLYHFGVSDFEEMTNINKVLRQKLAARCEIVAPEISSFQKSTDGTI
KFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTGQQGFNRNLTVSEIVGQIWRVSHFLGFAKDTGERPITN
VVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDAIDVALAVSIHAPNDELRDILVPINKKY
QLDEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKINLIPFNPYPGSPYGRSSNSRIDRFSK
VLMEYGFTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=366, Percent_Identity=75.9562841530055, Blast_Score=598, Evalue=1e-172,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41729; Mature: 41597

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKKINLLDLDRKAMRALFADMGEKPFRADQLMKWLYHFGVSDFEEMTNINKVLRQKLA
CCCCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ARCEIVAPEISSFQKSTDGTIKFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTF
HHHEEECCCHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
CSTGQQGFNRNLTVSEIVGQIWRVSHFLGFAKDTGERPITNVVMMGMGEPLLNLANVIPA
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHH
MDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDAIDVALAVSIHAPNDELRDILVPINKK
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCCCEEEEEEEEEECCCHHHHHHHCCCCCC
YQLDEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKINLIPF
CCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYGRSSNSRIDRFSKVLMEYGFTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KRMQENQISVTMN
HHHHCCCEEEEEC
>Mature Secondary Structure 
SEKKINLLDLDRKAMRALFADMGEKPFRADQLMKWLYHFGVSDFEEMTNINKVLRQKLA
CCCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ARCEIVAPEISSFQKSTDGTIKFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTF
HHHEEECCCHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
CSTGQQGFNRNLTVSEIVGQIWRVSHFLGFAKDTGERPITNVVMMGMGEPLLNLANVIPA
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHH
MDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDAIDVALAVSIHAPNDELRDILVPINKK
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCCCEEEEEEEEEECCCHHHHHHHCCCCCC
YQLDEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKINLIPF
CCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYGRSSNSRIDRFSKVLMEYGFTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KRMQENQISVTMN
HHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA