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The map label for this gene is rnfA [H]

Identifier: 126174475

GI number: 126174475

Start: 2616802

End: 2617380

Strand: Direct

Name: rnfA [H]

Synonym: Sbal_2259

Alternate gene names: 126174475

Gene position: 2616802-2617380 (Clockwise)

Preceding gene: 126174474

Following gene: 126174476

Centisome position: 51.04

GC content: 46.8

Gene sequence:

>579_bases
ATGACTGAATATCTCCTCTTGTTGATCAGTACAGTGCTGGTCAACAATTTCGTTTTAGTGAAATTCTTAGGCCTGTGCCC
TTTTATGGGGGTCTCCAGCAAATTGGAGTCCGCTATTGGCATGTCTATGGCGACCACTTTTGTGCTGACGTTAGCCTCTA
TTCTCAGTTATCTCGTCAACCAGTATCTGTTATTACCCTTTGACCTGAGTTATCTACGAACCATGAGCTTTATTTTAGTG
ATTGCCGTTGTTGTGCAATTTACTGAAATGGTCGTGCAAAAGACCAGTGCCGCTCTGCACCGTGCCTTAGGGATTTACCT
GCCGCTTATCACGACTAACTGCGCGGTATTGGGCGTAGCCTTATTAAACGTGAATGAGAAGCACGATTTTATCCAATCGG
CTATCTATGGCTTTGGTGCTGCGTTAGGCTTCTCATTAGTGTTAATTCTCTTCTCGGCGATGCGCGAGCGTTTAGCGGCC
GCCGATGTCCCCTTACCTTTCAAAGGCGGCGCGATTGCGATGATCACCGCAGGTCTTATGTCCCTCGCCTTTATGGGATT
CACAGGATTGGTTAAGTAA

Upstream 100 bases:

>100_bases
AAAATTTACAGTAAAGATGGTTAACCCTGCATTCTATATGTTGTTTATGCATACGGAAAAGTCGATAATACGGCCAAGAT
CCGTTTAGAAAAGTATTAGG

Downstream 100 bases:

>100_bases
GATGTCCGGAATTTTAATTGCAGTCATATTGTTGACCCTATTGGCATTAGTCTTTGGGGTATTGCTCGGTTTTGCCGCCC
TTAAATTCAAGGTCGAAGGC

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MTEYLLLLISTVLVNNFVLVKFLGLCPFMGVSSKLESAIGMSMATTFVLTLASILSYLVNQYLLLPFDLSYLRTMSFILV
IAVVVQFTEMVVQKTSAALHRALGIYLPLITTNCAVLGVALLNVNEKHDFIQSAIYGFGAALGFSLVLILFSAMRERLAA
ADVPLPFKGGAIAMITAGLMSLAFMGFTGLVK

Sequences:

>Translated_192_residues
MTEYLLLLISTVLVNNFVLVKFLGLCPFMGVSSKLESAIGMSMATTFVLTLASILSYLVNQYLLLPFDLSYLRTMSFILV
IAVVVQFTEMVVQKTSAALHRALGIYLPLITTNCAVLGVALLNVNEKHDFIQSAIYGFGAALGFSLVLILFSAMRERLAA
ADVPLPFKGGAIAMITAGLMSLAFMGFTGLVK
>Mature_191_residues
TEYLLLLISTVLVNNFVLVKFLGLCPFMGVSSKLESAIGMSMATTFVLTLASILSYLVNQYLLLPFDLSYLRTMSFILVI
AVVVQFTEMVVQKTSAALHRALGIYLPLITTNCAVLGVALLNVNEKHDFIQSAIYGFGAALGFSLVLILFSAMRERLAAA
DVPLPFKGGAIAMITAGLMSLAFMGFTGLVK

Specific function: May be part of a membrane complex involved in electron transport [H]

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787914, Length=192, Percent_Identity=75, Blast_Score=299, Evalue=8e-83,
Organism=Escherichia coli, GI1787919, Length=160, Percent_Identity=31.875, Blast_Score=67, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR011293 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 20715; Mature: 20584

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYLLLLISTVLVNNFVLVKFLGLCPFMGVSSKLESAIGMSMATTFVLTLASILSYLVN
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYLLLPFDLSYLRTMSFILVIAVVVQFTEMVVQKTSAALHRALGIYLPLITTNCAVLGVA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNVNEKHDFIQSAIYGFGAALGFSLVLILFSAMRERLAAADVPLPFKGGAIAMITAGLM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SLAFMGFTGLVK
HHHHHHHHHCCC
>Mature Secondary Structure 
TEYLLLLISTVLVNNFVLVKFLGLCPFMGVSSKLESAIGMSMATTFVLTLASILSYLVN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYLLLPFDLSYLRTMSFILVIAVVVQFTEMVVQKTSAALHRALGIYLPLITTNCAVLGVA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNVNEKHDFIQSAIYGFGAALGFSLVLILFSAMRERLAAADVPLPFKGGAIAMITAGLM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SLAFMGFTGLVK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA