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The map label for this gene is btuB [H]

Identifier: 126174266

GI number: 126174266

Start: 2369627

End: 2371756

Strand: Direct

Name: btuB [H]

Synonym: Sbal_2044

Alternate gene names: 126174266

Gene position: 2369627-2371756 (Clockwise)

Preceding gene: 126174265

Following gene: 126174271

Centisome position: 46.22

GC content: 43.9

Gene sequence:

>2130_bases
ATGATCAAAAGAACCAAAGTTGCGTCGGCTATTAACTTAGCTATCGTTGCATCTATCACTTCAGGTGCATTTGTTACGAC
AAGCGTATTTGCAGCGGAAGAAAAAGCAAAAGTTGAACGTATTGAAGTCACGGGTTCACGCATCCAACGCCAAGATATGG
AAACAGCTTCTCCTGTGACCGTTATTGATGCAGCCGCGATTAAAGCAGAAGGTTTCACTTCTGTAGATCAAATGTTACAA
GTGCAAACATCTATGGCGGGTGCAGCCATTGGTTCAACTACGAACAACGGTGCAGATGGTGTTGCGCAAGTTGATTTACG
TGGTATGGGCTCACAACGTACGCTTGTGTTGCTGAATGGCCGTCGTATGGTGAACTCAGGTTCTGGCGCCAATAGTTCTG
TCGATTTAAACTCTATCCCAGTTGCTATGATCGCCCGTGTTGAAATTCTGAAAGACGGCGCTTCGGCAGTTTACGGTTCA
GATGCCATCGCTGGTGTAGTCAACATTATCACTAAGAAAGACTTTGAGGGTTTCCAATTAGACTTTAACGGTAGTGGCAC
TGATAAAGGCGATGGCCGTAATGGCGACGTAAGTGCTTTATACGGTTTCAATACTGATGGTGGTAACTACACTATTGGTG
CCGCGTTCTCTGACCGCCGTGGTGTCATTCAGGGTGATCGTGATTGGACTGAACCTGGTGCAAGTTCATTTATTCCAACG
GGTACATTAGGTGGTAAAGTTCAAGATGCTAATGGTAACTGGGTAGCACGTAATACTGGTTACGATTTCACTCAAGATAG
TTATTATCAAACACCGAGTGAACGTCGTAGTTTATTTGCAAACATGACTCAAGAGCTAGGTAACGATGTTGTATTAACAG
CAGATGCTATTTATACCAACCGTCGATCTAACCAGCAAATGGCTGCTCAACCTGCTGACATTATGCTAAATGTTTGTGGC
GCAGCTGGCGTCGCAGCTGGTGCACCATGTGTAACACTTGATAGTGCGATGCTTGCTGCAGGTATTAAAGCCGATGATAA
AGGTTTAGTGAACTATCGTCGTCGTACTAACGATGTTGGTCCTCGTATTTATACTCAAGATACCGACACTTGGCGTTTAT
CAGGTGGTTTAGCAGGTACCTTAGATGTTCATACTGGTATGAACTGGGATCTAACTTACACCTATGGTAAAAACCAAGCT
AAAACCGCGGTAGATAACTCTATCAATGCATCAGACATGACGGCGTCAATTTACGCAAATCCAGATGCATGGTTTAGCGG
TGCACCGCTAACGCAGCAAATGATCAATGAAATTGGTTATTCTGAACAAGCCAATGGTGGTAACGAGCAACAAACCCTTT
CTGCAGGTTTAAATGGTGAATTGTTTGACTTGAAAGCGGGTGCAGTTGGCTTTGCTATTGGTGCTGAATACCGTCGCGAA
AGTGGTTTCTTTAATCCAGATCCAGTTGTTGTTGCTGGGGAAAGTACTGCGGCGCAACAGGATCCTACAGATGGTAACTA
CAACGTTATCTCTATTTTCCAAGAAGTGAGCGTGCCTTTCACTGAAAAATTAACGGGTGAATTTGCGCTGCGTTTAGATG
ATTACTCTACTTTCGGTAAAGCGACGACTTGGAAGATTGGTTTAACCTACACAGCAACAGATGATTTAATGATACGTGGT
GTGGCTGCTACTGGTTTCCGTGCACCAAGTGTGAGTGAATTATACGGCGGTAACTCTGGTTCATTTGATTACTTAGATGA
TCCATGGGGCAATGCTCAAGATCCACAAATTTTAGTAAACCGTACATCAGATCCAGATCTAAAACCTGAAGAATCAGAGC
CTTATACAACTGGTCTAGTCTATTCTCCAAGCTATGTTGATGGTATGTCAGTGACTCTTGACTATTGGCGTTTCAAAGTC
GGTGTTTTTCAAGATAGTTGTCACTCTTTGTTTAAAAATCAAGCTGCATTTATTACGCCTTCTACTGGTGCCTTATCTGG
GTTGAGTGTTACAGCGCCAACCGCTTCACAATTTCTAATCTCAGTAGACCAACGCATCGGTTTTCTTTCCTTCGCTGCCT
CAAGCACTTTCTTGCGCTTGGCTAAAATAGCGCCATCTTGCAGTGCATGA

Upstream 100 bases:

>100_bases
AATTAAAGTGAATCCTCAGTATCGACTGCAACACTAGTACAGCAATAGTTGTACAAGCTGTAAGCATAAAAATAATAGTA
AGACTAGGGAGCATTCATAC

Downstream 100 bases:

>100_bases
CGTTGCCCTGGTGAGACAAAATTTAGCTGGCTGTGTTTATGCTCATCGTTGTACCAGCTTACAAACTTTTCCACCCATTC
TCTTGCTTATAGCGCGTCAA

Product: TonB-dependent receptor, plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MIKRTKVASAINLAIVASITSGAFVTTSVFAAEEKAKVERIEVTGSRIQRQDMETASPVTVIDAAAIKAEGFTSVDQMLQ
VQTSMAGAAIGSTTNNGADGVAQVDLRGMGSQRTLVLLNGRRMVNSGSGANSSVDLNSIPVAMIARVEILKDGASAVYGS
DAIAGVVNIITKKDFEGFQLDFNGSGTDKGDGRNGDVSALYGFNTDGGNYTIGAAFSDRRGVIQGDRDWTEPGASSFIPT
GTLGGKVQDANGNWVARNTGYDFTQDSYYQTPSERRSLFANMTQELGNDVVLTADAIYTNRRSNQQMAAQPADIMLNVCG
AAGVAAGAPCVTLDSAMLAAGIKADDKGLVNYRRRTNDVGPRIYTQDTDTWRLSGGLAGTLDVHTGMNWDLTYTYGKNQA
KTAVDNSINASDMTASIYANPDAWFSGAPLTQQMINEIGYSEQANGGNEQQTLSAGLNGELFDLKAGAVGFAIGAEYRRE
SGFFNPDPVVVAGESTAAQQDPTDGNYNVISIFQEVSVPFTEKLTGEFALRLDDYSTFGKATTWKIGLTYTATDDLMIRG
VAATGFRAPSVSELYGGNSGSFDYLDDPWGNAQDPQILVNRTSDPDLKPEESEPYTTGLVYSPSYVDGMSVTLDYWRFKV
GVFQDSCHSLFKNQAAFITPSTGALSGLSVTAPTASQFLISVDQRIGFLSFAASSTFLRLAKIAPSCSA

Sequences:

>Translated_709_residues
MIKRTKVASAINLAIVASITSGAFVTTSVFAAEEKAKVERIEVTGSRIQRQDMETASPVTVIDAAAIKAEGFTSVDQMLQ
VQTSMAGAAIGSTTNNGADGVAQVDLRGMGSQRTLVLLNGRRMVNSGSGANSSVDLNSIPVAMIARVEILKDGASAVYGS
DAIAGVVNIITKKDFEGFQLDFNGSGTDKGDGRNGDVSALYGFNTDGGNYTIGAAFSDRRGVIQGDRDWTEPGASSFIPT
GTLGGKVQDANGNWVARNTGYDFTQDSYYQTPSERRSLFANMTQELGNDVVLTADAIYTNRRSNQQMAAQPADIMLNVCG
AAGVAAGAPCVTLDSAMLAAGIKADDKGLVNYRRRTNDVGPRIYTQDTDTWRLSGGLAGTLDVHTGMNWDLTYTYGKNQA
KTAVDNSINASDMTASIYANPDAWFSGAPLTQQMINEIGYSEQANGGNEQQTLSAGLNGELFDLKAGAVGFAIGAEYRRE
SGFFNPDPVVVAGESTAAQQDPTDGNYNVISIFQEVSVPFTEKLTGEFALRLDDYSTFGKATTWKIGLTYTATDDLMIRG
VAATGFRAPSVSELYGGNSGSFDYLDDPWGNAQDPQILVNRTSDPDLKPEESEPYTTGLVYSPSYVDGMSVTLDYWRFKV
GVFQDSCHSLFKNQAAFITPSTGALSGLSVTAPTASQFLISVDQRIGFLSFAASSTFLRLAKIAPSCSA
>Mature_709_residues
MIKRTKVASAINLAIVASITSGAFVTTSVFAAEEKAKVERIEVTGSRIQRQDMETASPVTVIDAAAIKAEGFTSVDQMLQ
VQTSMAGAAIGSTTNNGADGVAQVDLRGMGSQRTLVLLNGRRMVNSGSGANSSVDLNSIPVAMIARVEILKDGASAVYGS
DAIAGVVNIITKKDFEGFQLDFNGSGTDKGDGRNGDVSALYGFNTDGGNYTIGAAFSDRRGVIQGDRDWTEPGASSFIPT
GTLGGKVQDANGNWVARNTGYDFTQDSYYQTPSERRSLFANMTQELGNDVVLTADAIYTNRRSNQQMAAQPADIMLNVCG
AAGVAAGAPCVTLDSAMLAAGIKADDKGLVNYRRRTNDVGPRIYTQDTDTWRLSGGLAGTLDVHTGMNWDLTYTYGKNQA
KTAVDNSINASDMTASIYANPDAWFSGAPLTQQMINEIGYSEQANGGNEQQTLSAGLNGELFDLKAGAVGFAIGAEYRRE
SGFFNPDPVVVAGESTAAQQDPTDGNYNVISIFQEVSVPFTEKLTGEFALRLDDYSTFGKATTWKIGLTYTATDDLMIRG
VAATGFRAPSVSELYGGNSGSFDYLDDPWGNAQDPQILVNRTSDPDLKPEESEPYTTGLVYSPSYVDGMSVTLDYWRFKV
GVFQDSCHSLFKNQAAFITPSTGALSGLSVTAPTASQFLISVDQRIGFLSFAASSTFLRLAKIAPSCSA

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=180, Percent_Identity=31.1111111111111, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788478, Length=137, Percent_Identity=35.7664233576642, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1786798, Length=102, Percent_Identity=35.2941176470588, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 75265; Mature: 75265

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRTKVASAINLAIVASITSGAFVTTSVFAAEEKAKVERIEVTGSRIQRQDMETASPVT
CCCCHHHHHHHEEEEEEEECCCCEEEEEEHHHHHHCCEEEEEECHHHHHHHHCCCCCCEE
VIDAAAIKAEGFTSVDQMLQVQTSMAGAAIGSTTNNGADGVAQVDLRGMGSQRTLVLLNG
EEEHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEEECCCCCCEEEEEEC
RRMVNSGSGANSSVDLNSIPVAMIARVEILKDGASAVYGSDAIAGVVNIITKKDFEGFQL
CEEECCCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCEEE
DFNGSGTDKGDGRNGDVSALYGFNTDGGNYTIGAAFSDRRGVIQGDRDWTEPGASSFIPT
EECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCEECCCCCCCCCCCCCCCC
GTLGGKVQDANGNWVARNTGYDFTQDSYYQTPSERRSLFANMTQELGNDVVLTADAIYTN
CCCCCEEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHEECC
RRSNQQMAAQPADIMLNVCGAAGVAAGAPCVTLDSAMLAAGIKADDKGLVNYRRRTNDVG
CCCCCHHHCCCHHHHHHHHCCCCCCCCCCEEEECCHHHHCCCCCCCCCHHHHHHHCCCCC
PRIYTQDTDTWRLSGGLAGTLDVHTGMNWDLTYTYGKNQAKTAVDNSINASDMTASIYAN
CEEEECCCCCEEECCCCEEEEEECCCCCEEEEEEECCCCCHHHHCCCCCCCCEEEEEEEC
PDAWFSGAPLTQQMINEIGYSEQANGGNEQQTLSAGLNGELFDLKAGAVGFAIGAEYRRE
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCEEEEECCHHHHH
SGFFNPDPVVVAGESTAAQQDPTDGNYNVISIFQEVSVPFTEKLTGEFALRLDDYSTFGK
CCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCCHHHHCCCEEEEEECCCCCCCC
ATTWKIGLTYTATDDLMIRGVAATGFRAPSVSELYGGNSGSFDYLDDPWGNAQDPQILVN
CEEEEEEEEEEECCCEEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
RTSDPDLKPEESEPYTTGLVYSPSYVDGMSVTLDYWRFKVGVFQDSCHSLFKNQAAFITP
CCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEEEEEHHHHHHHHHCCCCEEEEC
STGALSGLSVTAPTASQFLISVDQRIGFLSFAASSTFLRLAKIAPSCSA
CCCCCCCCEEECCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIKRTKVASAINLAIVASITSGAFVTTSVFAAEEKAKVERIEVTGSRIQRQDMETASPVT
CCCCHHHHHHHEEEEEEEECCCCEEEEEEHHHHHHCCEEEEEECHHHHHHHHCCCCCCEE
VIDAAAIKAEGFTSVDQMLQVQTSMAGAAIGSTTNNGADGVAQVDLRGMGSQRTLVLLNG
EEEHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEEECCCCCCEEEEEEC
RRMVNSGSGANSSVDLNSIPVAMIARVEILKDGASAVYGSDAIAGVVNIITKKDFEGFQL
CEEECCCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCEEE
DFNGSGTDKGDGRNGDVSALYGFNTDGGNYTIGAAFSDRRGVIQGDRDWTEPGASSFIPT
EECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCEECCCCCCCCCCCCCCCC
GTLGGKVQDANGNWVARNTGYDFTQDSYYQTPSERRSLFANMTQELGNDVVLTADAIYTN
CCCCCEEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHEECC
RRSNQQMAAQPADIMLNVCGAAGVAAGAPCVTLDSAMLAAGIKADDKGLVNYRRRTNDVG
CCCCCHHHCCCHHHHHHHHCCCCCCCCCCEEEECCHHHHCCCCCCCCCHHHHHHHCCCCC
PRIYTQDTDTWRLSGGLAGTLDVHTGMNWDLTYTYGKNQAKTAVDNSINASDMTASIYAN
CEEEECCCCCEEECCCCEEEEEECCCCCEEEEEEECCCCCHHHHCCCCCCCCEEEEEEEC
PDAWFSGAPLTQQMINEIGYSEQANGGNEQQTLSAGLNGELFDLKAGAVGFAIGAEYRRE
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCEEEEECCHHHHH
SGFFNPDPVVVAGESTAAQQDPTDGNYNVISIFQEVSVPFTEKLTGEFALRLDDYSTFGK
CCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCCHHHHCCCEEEEEECCCCCCCC
ATTWKIGLTYTATDDLMIRGVAATGFRAPSVSELYGGNSGSFDYLDDPWGNAQDPQILVN
CEEEEEEEEEEECCCEEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
RTSDPDLKPEESEPYTTGLVYSPSYVDGMSVTLDYWRFKVGVFQDSCHSLFKNQAAFITP
CCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEEEEEHHHHHHHHHCCCCEEEEC
STGALSGLSVTAPTASQFLISVDQRIGFLSFAASSTFLRLAKIAPSCSA
CCCCCCCCEEECCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA