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The map label for this gene is pksE [H]

Identifier: 126173660

GI number: 126173660

Start: 1638851

End: 1640497

Strand: Reverse

Name: pksE [H]

Synonym: Sbal_1422

Alternate gene names: 126173660

Gene position: 1640497-1638851 (Counterclockwise)

Preceding gene: 126173661

Following gene: 126173659

Centisome position: 31.99

GC content: 51.97

Gene sequence:

>1647_bases
ATGACTAGCCATACTCTAGATCAATTCAATAGTAATAACGAAAAACTCAGCCCTTGGCCGTGGCAAGTCAACGATACTGA
GCTGAGCTTTGATATCGACTCATTAGGCAAAAAACTCAAAGAGTTAAGCCAAGCCTGCTACTTAGTGAATCACAGCGAAA
AAGGCTTAGGCATAGCGCAAACGGCCGAAGTGACCACCAGTGACAGCCAAGCGCCACTAGGCTCACACCCTGTCAGCGCC
TTTGCCCCTGCCCTTGGCACCCAAAGTTTAGGCGACAGTAATTTTCGCCGCGTCCACGGCGTTAAATACGCTTACTACGC
CGGCGCTATGGCAAACGGTATTGCCTCGGAAGAACTGGTTATCGCCCTTGGCCAAGCGGGTATTTTATGCTCATTTGGCG
CGGCGGGGTTAATTCCATCGCGGGTTGAAGCGGCCATCAAACGTATTCAAGCAGCGCTGCCCAATGGCCCCTACGCCTTT
AACTTAATTCACAGCCCAAGCGAACCCGCATTAGAGCGCGGCAGTGTGGAGCTGTTTTTAAGACATAAAGTGCGCACGGT
CGAAGCCTCAGCATTTTTAGGTTTAACGCCGCAGATCGTCTATTACCGCGCGGCGGGTTTGAGCCGCGACGAACATGGCG
AGATAGTCATTGGCAATAAAGTCATCGCAAAAATCAGCCGCACCGAAGTCGCGACTAAGTTTATGGAGCCAGCGCCGGCC
AAAATCCTCCAGCAATTAGTGAGTGACGGTCTTATCAGCCAAGATCAAATGGCGATGGCGCAACTCGTGCCAATGGCAGA
CGACATCACAGCCGAAGCCGACTCAGGCGGCCATACCGACAATCGTCCATTGGTCACGCTATTGCCGACGATTTTGGCGC
TCAAAGATGAAATCCAAGCTAAGTATCAATATAAAACGCCCATCCGTGTGGGCGCAGGCGGCGGCGTCGGCACACCCGAT
GCAGCATTAGCCACCTTCAACATGGGCGCGGCCTTTATCGTCACAGGTTCAATCAATCAAGCTTGCGTCGAAGCGGGCGC
GAGCGAACACACCCGTAAGTTACTCGCCACCACAGAAATGGCCGATGTGACTATGGCGCCTGCCGCTGATATGTTCGAAA
TGGGCGTGAAATTACAAGTGGTTAAGCGCGGCACTTTGTTCCCGATGCGCGCCAATAAGCTTTATGAGATCTACACCCGT
TACGATTCGATTGATGCCATCCCTGCGGACGAGCGTAAAAAGCTCGAAGAGCAAGTGTTCCGCTCATCACTCGATGACAT
TTGGGCGGGCACTGTCGCCCACTTTAACGAGCGCGATCCTAAGCAAATCGAGCGCGCACAGGATAATCCAAAACGTAAGA
TGGCGCTGATTTTCCGCTGGTATTTAGGTCTGTCGAGCCGCTGGTCAAACACAGGTGAAGTCGGTCGTGAAATGGATTAC
CAAATATGGGCAGGCCCTGCGCTTGGTGCATTTAACGCTTGGGCGAAAGGCAGTTATTTAGATGACTATAAAGCCCGTAA
TGCGGTGGATTTAGCCAAACATTTAATGGTGGGCGCAGCGTATCAATCCCGCATTAACTTGCTGTTATCCCAAGGCGCGA
GCATTCCGGTCACACTACTACGCTGGAAACCGCTGAATCGTTTTTAA

Upstream 100 bases:

>100_bases
TGAATCACTTAAAAATAATCCTGCCGCTGCGTTGGCTTTTTACACCCGAACTTTTTACTCAAGTAATAGCAACGCAGCAG
CATTAAAAACGAGACCCAAG

Downstream 100 bases:

>100_bases
CCGCTTTTAGCCCTATTGCAGGCTGCTAACAAAGCGAATTAATGCAAGAGCCGACACTGATTAATGAATGGATTAATTGA
TTCACGCATTAACAGTGTCG

Product: PfaD family protein

Products: NA

Alternate protein names: Malonyl CoA-acyl carrier protein transacylase; MCT [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MTSHTLDQFNSNNEKLSPWPWQVNDTELSFDIDSLGKKLKELSQACYLVNHSEKGLGIAQTAEVTTSDSQAPLGSHPVSA
FAPALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELVIALGQAGILCSFGAAGLIPSRVEAAIKRIQAALPNGPYAF
NLIHSPSEPALERGSVELFLRHKVRTVEASAFLGLTPQIVYYRAAGLSRDEHGEIVIGNKVIAKISRTEVATKFMEPAPA
KILQQLVSDGLISQDQMAMAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQAKYQYKTPIRVGAGGGVGTPD
AALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTR
YDSIDAIPADERKKLEEQVFRSSLDDIWAGTVAHFNERDPKQIERAQDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDY
QIWAGPALGAFNAWAKGSYLDDYKARNAVDLAKHLMVGAAYQSRINLLLSQGASIPVTLLRWKPLNRF

Sequences:

>Translated_548_residues
MTSHTLDQFNSNNEKLSPWPWQVNDTELSFDIDSLGKKLKELSQACYLVNHSEKGLGIAQTAEVTTSDSQAPLGSHPVSA
FAPALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELVIALGQAGILCSFGAAGLIPSRVEAAIKRIQAALPNGPYAF
NLIHSPSEPALERGSVELFLRHKVRTVEASAFLGLTPQIVYYRAAGLSRDEHGEIVIGNKVIAKISRTEVATKFMEPAPA
KILQQLVSDGLISQDQMAMAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQAKYQYKTPIRVGAGGGVGTPD
AALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTR
YDSIDAIPADERKKLEEQVFRSSLDDIWAGTVAHFNERDPKQIERAQDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDY
QIWAGPALGAFNAWAKGSYLDDYKARNAVDLAKHLMVGAAYQSRINLLLSQGASIPVTLLRWKPLNRF
>Mature_547_residues
TSHTLDQFNSNNEKLSPWPWQVNDTELSFDIDSLGKKLKELSQACYLVNHSEKGLGIAQTAEVTTSDSQAPLGSHPVSAF
APALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELVIALGQAGILCSFGAAGLIPSRVEAAIKRIQAALPNGPYAFN
LIHSPSEPALERGSVELFLRHKVRTVEASAFLGLTPQIVYYRAAGLSRDEHGEIVIGNKVIAKISRTEVATKFMEPAPAK
ILQQLVSDGLISQDQMAMAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQAKYQYKTPIRVGAGGGVGTPDA
ALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRY
DSIDAIPADERKKLEEQVFRSSLDDIWAGTVAHFNERDPKQIERAQDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDYQ
IWAGPALGAFNAWAKGSYLDDYKARNAVDLAKHLMVGAAYQSRINLLLSQGASIPVTLLRWKPLNRF

Specific function: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductas

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the fabD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR001227
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013785
- InterPro:   IPR004410
- InterPro:   IPR016036
- InterPro:   IPR014179 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF03060 NPD [H]

EC number: =2.3.1.39 [H]

Molecular weight: Translated: 59746; Mature: 59615

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHTLDQFNSNNEKLSPWPWQVNDTELSFDIDSLGKKLKELSQACYLVNHSEKGLGIAQ
CCCCCCHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHEEEECCCCCCCCEE
TAEVTTSDSQAPLGSHPVSAFAPALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELV
ECEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEE
IALGQAGILCSFGAAGLIPSRVEAAIKRIQAALPNGPYAFNLIHSPSEPALERGSVELFL
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCCEEEEE
RHKVRTVEASAFLGLTPQIVYYRAAGLSRDEHGEIVIGNKVIAKISRTEVATKFMEPAPA
EHHHHEEEHHHHCCCCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCHH
KILQQLVSDGLISQDQMAMAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQA
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KYQYKTPIRVGAGGGVGTPDAALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEM
EEEECCCEEEECCCCCCCCCHHEEEECCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHH
ADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRYDSIDAIPADERKKLEEQVF
HHEEECCCHHHHHCCCEEEEEECCCCCEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
RSSLDDIWAGTVAHFNERDPKQIERAQDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDY
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
QIWAGPALGAFNAWAKGSYLDDYKARNAVDLAKHLMVGAAYQSRINLLLSQGASIPVTLL
EEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
RWKPLNRF
ECCCCCCC
>Mature Secondary Structure 
TSHTLDQFNSNNEKLSPWPWQVNDTELSFDIDSLGKKLKELSQACYLVNHSEKGLGIAQ
CCCCCHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHEEEECCCCCCCCEE
TAEVTTSDSQAPLGSHPVSAFAPALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELV
ECEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEE
IALGQAGILCSFGAAGLIPSRVEAAIKRIQAALPNGPYAFNLIHSPSEPALERGSVELFL
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCCEEEEE
RHKVRTVEASAFLGLTPQIVYYRAAGLSRDEHGEIVIGNKVIAKISRTEVATKFMEPAPA
EHHHHEEEHHHHCCCCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCHH
KILQQLVSDGLISQDQMAMAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQA
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KYQYKTPIRVGAGGGVGTPDAALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEM
EEEECCCEEEECCCCCCCCCHHEEEECCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHH
ADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRYDSIDAIPADERKKLEEQVF
HHEEECCCHHHHHCCCEEEEEECCCCCEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
RSSLDDIWAGTVAHFNERDPKQIERAQDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDY
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
QIWAGPALGAFNAWAKGSYLDDYKARNAVDLAKHLMVGAAYQSRINLLLSQGASIPVTLL
EEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
RWKPLNRF
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]