Click here to switch to the map view.

The map label for this gene is grpE [H]

Identifier: 125624377

GI number: 125624377

Start: 1551527

End: 1552066

Strand: Reverse

Name: grpE [H]

Synonym: llmg_1575

Alternate gene names: 125624377

Gene position: 1552066-1551527 (Counterclockwise)

Preceding gene: 125624378

Following gene: 125624376

Centisome position: 61.36

GC content: 38.33

Gene sequence:

>540_bases
ATGTCCGAAGAAACTAAAGAAGAAATCAAAAACGAAAAAGTTGATGAAGAAGTGACTGAGGAGTTAACAGAAGAAGCGCT
TGAAGATATCGTTGAAGAAGAAATCAATGAACTTGATGAAGCGCAAAAGTTAGCAACTGAGTGGGAAAATAAATTCCTTC
GTGTTTCTGCTGAAATGCAAAATGTGCAACGTCGCGGAAATGAAGAACGTTTACAACTTGTCAAGTATCGCTCGCAAGAT
TTAGCAAAAAAAATCTTGTCTTCATTAGATAATCTTGAACGTGCACTTGCTGTTGAAGGTTTGACAGATGATGTTAAAAA
AGGGCTTGAAATGGTACAAGAAAGTTTAATTTCAGCCCTTAAAGAAGAAGGGGTTGAAGAAGTTTCTTATGAAAGTTTCG
ACCATAACCTTCACATGGCTGTTCAAACTGTTCCAGCTGATGACGAACATCCAGCTGACAGCATTGTGCAAGTCTTCCAA
AAAGGCTACCAACTTCACGAACGTTTACTACGTCCTGCAATGGTTGTTGTAGCACAATAA

Upstream 100 bases:

>100_bases
AAGTTCTAACAATGAAATTAAGTGATTATTATCGTTACCTTGATGGTAACCATTATGAAATTAGTAAATGATTAAAAAGT
TATTTAAAAAAGGAGGGCCT

Downstream 100 bases:

>100_bases
TTTAATAAAAAATATTGACCGCAAAGTCTTTAAACTATTCATAAGTAAAAGAAATAAAGGAGATTTAAAATATGTCTAAA
ATTATCGGTATTGACCTTGG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MSEETKEEIKNEKVDEEVTEELTEEALEDIVEEEINELDEAQKLATEWENKFLRVSAEMQNVQRRGNEERLQLVKYRSQD
LAKKILSSLDNLERALAVEGLTDDVKKGLEMVQESLISALKEEGVEEVSYESFDHNLHMAVQTVPADDEHPADSIVQVFQ
KGYQLHERLLRPAMVVVAQ

Sequences:

>Translated_179_residues
MSEETKEEIKNEKVDEEVTEELTEEALEDIVEEEINELDEAQKLATEWENKFLRVSAEMQNVQRRGNEERLQLVKYRSQD
LAKKILSSLDNLERALAVEGLTDDVKKGLEMVQESLISALKEEGVEEVSYESFDHNLHMAVQTVPADDEHPADSIVQVFQ
KGYQLHERLLRPAMVVVAQ
>Mature_178_residues
SEETKEEIKNEKVDEEVTEELTEEALEDIVEEEINELDEAQKLATEWENKFLRVSAEMQNVQRRGNEERLQLVKYRSQDL
AKKILSSLDNLERALAVEGLTDDVKKGLEMVQESLISALKEEGVEEVSYESFDHNLHMAVQTVPADDEHPADSIVQVFQK
GYQLHERLLRPAMVVVAQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=149, Percent_Identity=32.2147651006711, Blast_Score=72, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324806, Length=181, Percent_Identity=29.2817679558011, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 20567; Mature: 20436

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEETKEEIKNEKVDEEVTEELTEEALEDIVEEEINELDEAQKLATEWENKFLRVSAEMQ
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVQRRGNEERLQLVKYRSQDLAKKILSSLDNLERALAVEGLTDDVKKGLEMVQESLISAL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KEEGVEEVSYESFDHNLHMAVQTVPADDEHPADSIVQVFQKGYQLHERLLRPAMVVVAQ
HHCCHHHHHHHHHCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEETKEEIKNEKVDEEVTEELTEEALEDIVEEEINELDEAQKLATEWENKFLRVSAEMQ
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVQRRGNEERLQLVKYRSQDLAKKILSSLDNLERALAVEGLTDDVKKGLEMVQESLISAL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KEEGVEEVSYESFDHNLHMAVQTVPADDEHPADSIVQVFQKGYQLHERLLRPAMVVVAQ
HHCCHHHHHHHHHCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]