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The map label for this gene is prfB [H]

Identifier: 125624350

GI number: 125624350

Start: 1519901

End: 1520998

Strand: Reverse

Name: prfB [H]

Synonym: llmg_1547

Alternate gene names: 125624350

Gene position: 1520998-1519901 (Counterclockwise)

Preceding gene: 125624354

Following gene: 125624349

Centisome position: 60.13

GC content: 40.44

Gene sequence:

>1098_bases
ATGGAATTATCAGAAATTAGAAATTTACTTGAAGGATATAGCGAGAAGATTAATGGCTTTCGTGACTCCTTAGATCTTGA
CCGTTTGGAAGAAGAAATTGCTTTATTAGAAAATGATATGGCGCAGCCTGAATTTTGGAATGATCAAGCAGCGGCTCAAA
AAGTAATTGACGAATCAAATGCTTTAAAAGCAAAATACGACAATTATCAGGCCATGAATACCATGCTTGAAGAAGCTCAA
ACCATGCTTGAAATGCTTCAGGAAGAAGCTGATGAAGACATGCAAGTCGAATTAGAAGAGATGACCACAGCTCTTGGTCA
AAAAATTGAATCTTACGAACTCGAAATCATGCTAAATCAGCCTTATGACCACATGAATGCGGTACTTGAAATCCATCCGG
GTTCTGGTGGAACAGAGTCGCAGGACTGGGGCTCAATGCTCATGCGGATGTATACCCGCTGGGGTGAAGCGCATGGCTTT
AAAGTAGAAATTTTGGACTATCAAGATGGAGATGTTGCTGGACTTAAATCAGTAGCCATTCGTTTTGTTGGACGAAATGC
ATATGGTTTTTTGCGCGGCGAAAAAGGAGTTCATCGTTTAGTTCGCATATCGCCATTTGATTCTGCTAATCGTCGTCATA
CTTCATTTACTTCAGTAGACGTGATGCCAGAACTGGATGATTCGATTGAAGTTGAAGTTCGTGATGCTGATGTGAAAATG
GATACCTTCCGTTCTGGAGGAGCAGGTGGACAAAATGTCAATAAAGTATCCACAGGGGTTCGCTTGACTCACGTTCCAAC
AGGGATTGTTGTCCAATCAACAATGGATAGAACACAATATGGTAACCGTGACAAGGCCATGGCTATGTTGAAGTCAAAAC
TCTATCAATTAGAAATGGATAAAAAACAAGCAGAAGTTGATGAACTTCGAGGAGACCAATCAGAAATCTCTTGGGGAAGT
CAAATTCGTTCTTATGTTTTCATGCCATATCAGTTAGTCAAAGACACACGAACTGGTTATGAAACAGGACAAATTTCAAA
CGTTATGGACGGAGAAATAGACGGATTTATCAATGCTTACCTTCGTTGGAACCTCTAA

Upstream 100 bases:

>100_bases
ATATAATTTTTGAGATTCCTATTATAAAAAAGTTTAAGTCCATTTTCTATTGAATAGCTTAGGTTAGGCTTATAGGCGGT
GAATATGGTATAATGCAACT

Downstream 100 bases:

>100_bases
AATTAAGCTGGTTCTTGGAGTTATTGAAATGATTACGAAACATTAAAGTTATTAATGAAAAGATTATAAAATAAAAGTGC
TACCTAAAGCACTTTTATTT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MELSEIRNLLEGYSEKINGFRDSLDLDRLEEEIALLENDMAQPEFWNDQAAAQKVIDESNALKAKYDNYQAMNTMLEEAQ
TMLEMLQEEADEDMQVELEEMTTALGQKIESYELEIMLNQPYDHMNAVLEIHPGSGGTESQDWGSMLMRMYTRWGEAHGF
KVEILDYQDGDVAGLKSVAIRFVGRNAYGFLRGEKGVHRLVRISPFDSANRRHTSFTSVDVMPELDDSIEVEVRDADVKM
DTFRSGGAGGQNVNKVSTGVRLTHVPTGIVVQSTMDRTQYGNRDKAMAMLKSKLYQLEMDKKQAEVDELRGDQSEISWGS
QIRSYVFMPYQLVKDTRTGYETGQISNVMDGEIDGFINAYLRWNL

Sequences:

>Translated_365_residues
MELSEIRNLLEGYSEKINGFRDSLDLDRLEEEIALLENDMAQPEFWNDQAAAQKVIDESNALKAKYDNYQAMNTMLEEAQ
TMLEMLQEEADEDMQVELEEMTTALGQKIESYELEIMLNQPYDHMNAVLEIHPGSGGTESQDWGSMLMRMYTRWGEAHGF
KVEILDYQDGDVAGLKSVAIRFVGRNAYGFLRGEKGVHRLVRISPFDSANRRHTSFTSVDVMPELDDSIEVEVRDADVKM
DTFRSGGAGGQNVNKVSTGVRLTHVPTGIVVQSTMDRTQYGNRDKAMAMLKSKLYQLEMDKKQAEVDELRGDQSEISWGS
QIRSYVFMPYQLVKDTRTGYETGQISNVMDGEIDGFINAYLRWNL
>Mature_365_residues
MELSEIRNLLEGYSEKINGFRDSLDLDRLEEEIALLENDMAQPEFWNDQAAAQKVIDESNALKAKYDNYQAMNTMLEEAQ
TMLEMLQEEADEDMQVELEEMTTALGQKIESYELEIMLNQPYDHMNAVLEIHPGSGGTESQDWGSMLMRMYTRWGEAHGF
KVEILDYQDGDVAGLKSVAIRFVGRNAYGFLRGEKGVHRLVRISPFDSANRRHTSFTSVDVMPELDDSIEVEVRDADVKM
DTFRSGGAGGQNVNKVSTGVRLTHVPTGIVVQSTMDRTQYGNRDKAMAMLKSKLYQLEMDKKQAEVDELRGDQSEISWGS
QIRSYVFMPYQLVKDTRTGYETGQISNVMDGEIDGFINAYLRWNL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=308, Percent_Identity=32.7922077922078, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI166795303, Length=303, Percent_Identity=29.042904290429, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI166795305, Length=213, Percent_Identity=29.1079812206573, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI2367172, Length=365, Percent_Identity=45.4794520547945, Blast_Score=328, Evalue=2e-91,
Organism=Escherichia coli, GI1787462, Length=303, Percent_Identity=35.6435643564356, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17542784, Length=252, Percent_Identity=31.3492063492063, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=317, Percent_Identity=28.0757097791798, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19921226, Length=260, Percent_Identity=31.1538461538462, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41547; Mature: 41547

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSEIRNLLEGYSEKINGFRDSLDLDRLEEEIALLENDMAQPEFWNDQAAAQKVIDESN
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
ALKAKYDNYQAMNTMLEEAQTMLEMLQEEADEDMQVELEEMTTALGQKIESYELEIMLNQ
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCEEEEEEECC
PYDHMNAVLEIHPGSGGTESQDWGSMLMRMYTRWGEAHGFKVEILDYQDGDVAGLKSVAI
CHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
RFVGRNAYGFLRGEKGVHRLVRISPFDSANRRHTSFTSVDVMPELDDSIEVEVRDADVKM
HHCCCCCEEEECCCCCHHHEEEECCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCEEE
DTFRSGGAGGQNVNKVSTGVRLTHVPTGIVVQSTMDRTQYGNRDKAMAMLKSKLYQLEMD
HHCCCCCCCCCCCHHHHCCEEEEECCCCEEEECHHHHHHCCCHHHHHHHHHHHHHHHHCC
KKQAEVDELRGDQSEISWGSQIRSYVFMPYQLVKDTRTGYETGQISNVMDGEIDGFINAY
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEE
LRWNL
EEECC
>Mature Secondary Structure
MELSEIRNLLEGYSEKINGFRDSLDLDRLEEEIALLENDMAQPEFWNDQAAAQKVIDESN
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
ALKAKYDNYQAMNTMLEEAQTMLEMLQEEADEDMQVELEEMTTALGQKIESYELEIMLNQ
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCEEEEEEECC
PYDHMNAVLEIHPGSGGTESQDWGSMLMRMYTRWGEAHGFKVEILDYQDGDVAGLKSVAI
CHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
RFVGRNAYGFLRGEKGVHRLVRISPFDSANRRHTSFTSVDVMPELDDSIEVEVRDADVKM
HHCCCCCEEEECCCCCHHHEEEECCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCEEE
DTFRSGGAGGQNVNKVSTGVRLTHVPTGIVVQSTMDRTQYGNRDKAMAMLKSKLYQLEMD
HHCCCCCCCCCCCHHHHCCEEEEECCCCEEEECHHHHHHCCCHHHHHHHHHHHHHHHHCC
KKQAEVDELRGDQSEISWGSQIRSYVFMPYQLVKDTRTGYETGQISNVMDGEIDGFINAY
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEE
LRWNL
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]