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The map label for this gene is apbE [H]

Identifier: 125624258

GI number: 125624258

Start: 1418101

End: 1419186

Strand: Reverse

Name: apbE [H]

Synonym: llmg_1450

Alternate gene names: 125624258

Gene position: 1419186-1418101 (Counterclockwise)

Preceding gene: 125624259

Following gene: 125624257

Centisome position: 56.11

GC content: 33.43

Gene sequence:

>1086_bases
ATGAAAAAAATAATAATTTGGGTTGCAGCTGCATTGAGTTTACTAAGTCTAAGCGCTTGTAGCAATGGAAAATCGAGTCA
AAAAGATAATCGTGATTTATTAAAAACACCAATCAATAAAGATGTGACAACCATGGGAACTTACATCAAAGTCACTGTTT
ATGACAAGGATAAAGAAGCAGCAGTTGATAAAGCCTTAAAACTTCCTGTCGAATATAATAAATTAACTACTGTTAATCAA
TCGGGTTCACAAATTGATGATATTAATCAAAATGCGGGAATCAAACCTGTCAAAGTAGACGCAAGTGTCTATGAATTGGT
TAAAGCTGGTTACAATTATTCAGCTAAAGATTTAGGTTATGATATTACCATTGGTTCTCTGACAAGTCTGTGGAATATTG
GGATGGATAATGCTAGAAAACCTGCTCAATCAGAAATTGACCAAGTTTTGCCCTTGATTAATTATCATGATATAAAATTT
AATGATAAAAATCAGACGATTTATCTGACTAAAAAAGGTGTTCGCTTAGACTTGGGCTCAATTGTTAAAGGTTTTGTTGC
TATGAAAATGGTGGATTCACTCAAAAAAGATGGGGTGACCACAGGAATCGTGAATCTTGGCTCTAGTTCAATTTATGTCA
TTGGTCATTCACCGCGTGGGGCTGACAGTCCTTGGACGATTGGTATCAAGAATCCAAATGACCCGACTGGAACGCAAATG
GGGATTATTGAAGCTTCAAATGAACATGTCAATACCTCAGGAATCTATGAGCGTTATTTAGAAGTTGATGGTCATAAATA
TAGTCACATTTTAAATCCTAAAACAGGTTACCCATTTGAAAATGATATTGCAAGCATTACCTTATTAATTTCTGGCAAAG
ATAAGACAAATGGTGACGGTTTATCAACCATGATTTATGCTATGGGGACTAAAAAAGGCTATGAATATGTTGAAAAATTA
AAAAATGTAGAGGCCGTATTTGTTGATAAAAACAACAAAGTCTATATCACTCCAGGATTAAAAGATAAATTTCAACTTTC
AGAAAAGACTTCTTTTAAAATCGAAAATATTACAAACTTAAAATAA

Upstream 100 bases:

>100_bases
TGACACAAATGAAGAATAAATAAAATGAAAGTCTGCCAGTTACTGACAGACTTTCTGATTAAATAAATGGTGCTGACTAG
TCTGTCAGTAAAAATTGAAC

Downstream 100 bases:

>100_bases
TTGTTTAAATCTTGCTATATGCAAGATTTTTTTGTAAAAGAATTAAAACTTTTGACTTGGAACTTAAATCGTGATACAAT
AAGTGGAAAGAGTACATTAA

Product: thiamine biosynthesis lipoprotein apbE precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKKIIIWVAAALSLLSLSACSNGKSSQKDNRDLLKTPINKDVTTMGTYIKVTVYDKDKEAAVDKALKLPVEYNKLTTVNQ
SGSQIDDINQNAGIKPVKVDASVYELVKAGYNYSAKDLGYDITIGSLTSLWNIGMDNARKPAQSEIDQVLPLINYHDIKF
NDKNQTIYLTKKGVRLDLGSIVKGFVAMKMVDSLKKDGVTTGIVNLGSSSIYVIGHSPRGADSPWTIGIKNPNDPTGTQM
GIIEASNEHVNTSGIYERYLEVDGHKYSHILNPKTGYPFENDIASITLLISGKDKTNGDGLSTMIYAMGTKKGYEYVEKL
KNVEAVFVDKNNKVYITPGLKDKFQLSEKTSFKIENITNLK

Sequences:

>Translated_361_residues
MKKIIIWVAAALSLLSLSACSNGKSSQKDNRDLLKTPINKDVTTMGTYIKVTVYDKDKEAAVDKALKLPVEYNKLTTVNQ
SGSQIDDINQNAGIKPVKVDASVYELVKAGYNYSAKDLGYDITIGSLTSLWNIGMDNARKPAQSEIDQVLPLINYHDIKF
NDKNQTIYLTKKGVRLDLGSIVKGFVAMKMVDSLKKDGVTTGIVNLGSSSIYVIGHSPRGADSPWTIGIKNPNDPTGTQM
GIIEASNEHVNTSGIYERYLEVDGHKYSHILNPKTGYPFENDIASITLLISGKDKTNGDGLSTMIYAMGTKKGYEYVEKL
KNVEAVFVDKNNKVYITPGLKDKFQLSEKTSFKIENITNLK
>Mature_361_residues
MKKIIIWVAAALSLLSLSACSNGKSSQKDNRDLLKTPINKDVTTMGTYIKVTVYDKDKEAAVDKALKLPVEYNKLTTVNQ
SGSQIDDINQNAGIKPVKVDASVYELVKAGYNYSAKDLGYDITIGSLTSLWNIGMDNARKPAQSEIDQVLPLINYHDIKF
NDKNQTIYLTKKGVRLDLGSIVKGFVAMKMVDSLKKDGVTTGIVNLGSSSIYVIGHSPRGADSPWTIGIKNPNDPTGTQM
GIIEASNEHVNTSGIYERYLEVDGHKYSHILNPKTGYPFENDIASITLLISGKDKTNGDGLSTMIYAMGTKKGYEYVEKL
KNVEAVFVDKNNKVYITPGLKDKFQLSEKTSFKIENITNLK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=317, Percent_Identity=23.3438485804416, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 39751; Mature: 39751

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIIWVAAALSLLSLSACSNGKSSQKDNRDLLKTPINKDVTTMGTYIKVTVYDKDKEA
CCEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCEECCEEEEEEEEECCHHH
AVDKALKLPVEYNKLTTVNQSGSQIDDINQNAGIKPVKVDASVYELVKAGYNYSAKDLGY
HHHHHHCCCEECCEEEEECCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCHHHCCE
DITIGSLTSLWNIGMDNARKPAQSEIDQVLPLINYHDIKFNDKNQTIYLTKKGVRLDLGS
EEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCEEEHHH
IVKGFVAMKMVDSLKKDGVTTGIVNLGSSSIYVIGHSPRGADSPWTIGIKNPNDPTGTQM
HHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCEE
GIIEASNEHVNTSGIYERYLEVDGHKYSHILNPKTGYPFENDIASITLLISGKDKTNGDG
EEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCC
LSTMIYAMGTKKGYEYVEKLKNVEAVFVDKNNKVYITPGLKDKFQLSEKTSFKIENITNL
CEEEEEECCCCHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCEECCCCCCEEECCCCCC
K
C
>Mature Secondary Structure
MKKIIIWVAAALSLLSLSACSNGKSSQKDNRDLLKTPINKDVTTMGTYIKVTVYDKDKEA
CCEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCEECCEEEEEEEEECCHHH
AVDKALKLPVEYNKLTTVNQSGSQIDDINQNAGIKPVKVDASVYELVKAGYNYSAKDLGY
HHHHHHCCCEECCEEEEECCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCHHHCCE
DITIGSLTSLWNIGMDNARKPAQSEIDQVLPLINYHDIKFNDKNQTIYLTKKGVRLDLGS
EEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCEEEHHH
IVKGFVAMKMVDSLKKDGVTTGIVNLGSSSIYVIGHSPRGADSPWTIGIKNPNDPTGTQM
HHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCEE
GIIEASNEHVNTSGIYERYLEVDGHKYSHILNPKTGYPFENDIASITLLISGKDKTNGDG
EEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCC
LSTMIYAMGTKKGYEYVEKLKNVEAVFVDKNNKVYITPGLKDKFQLSEKTSFKIENITNL
CEEEEEECCCCHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCEECCCCCCEEECCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]