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The map label for this gene is rluB [H]

Identifier: 125624024

GI number: 125624024

Start: 1163540

End: 1164313

Strand: Direct

Name: rluB [H]

Synonym: llmg_1194

Alternate gene names: 125624024

Gene position: 1163540-1164313 (Clockwise)

Preceding gene: 125624023

Following gene: 125624025

Centisome position: 46.0

GC content: 37.98

Gene sequence:

>774_bases
ATGAGTCAAGATATGTGGCAGAACAACAAACCCTATTTAACGAAAGTGAAGAAAATGAGAATCAATAAATATTTGGCCCA
TGCAGGAGTGGCAAGCAGACGTAAAGCAGAAGAACTAATTTTGGCGGGAAAAGTGACAGTTAATAATGTCCCGATGACAA
ATTTAGGTTATCAAGTTTCAACTGGAGATGTCGTCGAAGTTAATGGAGTGGCAGTTTATAATGAAGAACCTGTTTATTAT
CTATTGAACAAACCAAGAGGTTATATTTCTTCTGTCAGTGATGATAAAGGACGTCAAACGGTGATGGATTTAATGCCTCA
AGTTAAAGAACGTATCTATCCAGTAGGGCGTTTGGACTGGGATACTAGTGGCTTACTTTTACTCACTAACGATGGTGAAT
TCACAAATATGATGACTCACCCAAGTCACGGTGTTGATAAAGTTTATGTGGCCAAGGTTGAAGGACAAGCGAACAAGGAA
AACTTGCGTCCCTTAACACTTGGAATGACGATTGAAGGTAAGAAAGTTAGCCCGGCTCGTTATGAAATTATCAAACAAGA
AAAAACAAAAAACCATTCAATTGTTAGCTTGACGATTCATGAAGGTCAAAATCATCAAGTTAAGAAGATGTTTGCTGCTG
CTGGACTTCCAGTACAAAAATTAAGTCGTATTCAATATGGAACACTTACATTGGATGGACTGCCTACAGGACAATTCCGT
CGTTTGAGTAAAAAAGAAGTTTCTCAATTGATTAATGCTGCGCAAGGAAAATAG

Upstream 100 bases:

>100_bases
GGGCAAGTTGAAGCACCGGGACGTCCTAGCCTTTATGCGACTACTGAATTTTTCCTAGATTATATTGGTATCAATAATTT
AGAAGAATTACCTGAAATTG

Downstream 100 bases:

>100_bases
TTTAAATAAAATAAAGACAATTCTCTATAAAGAGTTGTTTTTTTATTTCTTAAATAAATCTTATTCTTGATATGCTAAGT
ATTTTTGTGCTATAATAAAA

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSQDMWQNNKPYLTKVKKMRINKYLAHAGVASRRKAEELILAGKVTVNNVPMTNLGYQVSTGDVVEVNGVAVYNEEPVYY
LLNKPRGYISSVSDDKGRQTVMDLMPQVKERIYPVGRLDWDTSGLLLLTNDGEFTNMMTHPSHGVDKVYVAKVEGQANKE
NLRPLTLGMTIEGKKVSPARYEIIKQEKTKNHSIVSLTIHEGQNHQVKKMFAAAGLPVQKLSRIQYGTLTLDGLPTGQFR
RLSKKEVSQLINAAQGK

Sequences:

>Translated_257_residues
MSQDMWQNNKPYLTKVKKMRINKYLAHAGVASRRKAEELILAGKVTVNNVPMTNLGYQVSTGDVVEVNGVAVYNEEPVYY
LLNKPRGYISSVSDDKGRQTVMDLMPQVKERIYPVGRLDWDTSGLLLLTNDGEFTNMMTHPSHGVDKVYVAKVEGQANKE
NLRPLTLGMTIEGKKVSPARYEIIKQEKTKNHSIVSLTIHEGQNHQVKKMFAAAGLPVQKLSRIQYGTLTLDGLPTGQFR
RLSKKEVSQLINAAQGK
>Mature_256_residues
SQDMWQNNKPYLTKVKKMRINKYLAHAGVASRRKAEELILAGKVTVNNVPMTNLGYQVSTGDVVEVNGVAVYNEEPVYYL
LNKPRGYISSVSDDKGRQTVMDLMPQVKERIYPVGRLDWDTSGLLLLTNDGEFTNMMTHPSHGVDKVYVAKVEGQANKEN
LRPLTLGMTIEGKKVSPARYEIIKQEKTKNHSIVSLTIHEGQNHQVKKMFAAAGLPVQKLSRIQYGTLTLDGLPTGQFRR
LSKKEVSQLINAAQGK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=233, Percent_Identity=34.7639484978541, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=33.1932773109244, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=32.6271186440678, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=32.3863636363636, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 28771; Mature: 28639

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDMWQNNKPYLTKVKKMRINKYLAHAGVASRRKAEELILAGKVTVNNVPMTNLGYQVS
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECEEEECCCCCCCCCEEEC
TGDVVEVNGVAVYNEEPVYYLLNKPRGYISSVSDDKGRQTVMDLMPQVKERIYPVGRLDW
CCCEEEECCEEEECCCCEEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
DTSGLLLLTNDGEFTNMMTHPSHGVDKVYVAKVEGQANKENLRPLTLGMTIEGKKVSPAR
CCCCEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCHHH
YEIIKQEKTKNHSIVSLTIHEGQNHQVKKMFAAAGLPVQKLSRIQYGTLTLDGLPTGQFR
HHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHEEECEEEECCCCCHHHH
RLSKKEVSQLINAAQGK
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQDMWQNNKPYLTKVKKMRINKYLAHAGVASRRKAEELILAGKVTVNNVPMTNLGYQVS
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECEEEECCCCCCCCCEEEC
TGDVVEVNGVAVYNEEPVYYLLNKPRGYISSVSDDKGRQTVMDLMPQVKERIYPVGRLDW
CCCEEEECCEEEECCCCEEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
DTSGLLLLTNDGEFTNMMTHPSHGVDKVYVAKVEGQANKENLRPLTLGMTIEGKKVSPAR
CCCCEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCHHH
YEIIKQEKTKNHSIVSLTIHEGQNHQVKKMFAAAGLPVQKLSRIQYGTLTLDGLPTGQFR
HHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHEEECEEEECCCCCHHHH
RLSKKEVSQLINAAQGK
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]