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The map label for this gene is gltD [H]

Identifier: 125624014

GI number: 125624014

Start: 1155761

End: 1157200

Strand: Direct

Name: gltD [H]

Synonym: llmg_1184

Alternate gene names: 125624014

Gene position: 1155761-1157200 (Clockwise)

Preceding gene: 125624013

Following gene: 125624015

Centisome position: 45.69

GC content: 41.39

Gene sequence:

>1440_bases
ATGGCTGATCCAAATGGATTTTTAAATTATCCAAGAAATGATAATCCTTACCGTGAATTAGCGGAGCGGATAAAAGATTT
TGCTGAATTGCAAGTTCCCTTATCCACTGAAGAACGACAAAAACAAGCGGCACGTTGTATGCATTGTGATGTTCCTTTTT
GTCATCAAGGGATATTTTATGGGGGCAAACGAGCTGTCTCAGGCTGTCCAAATGATAATCATATTCCAGAGTGGAATGAT
TTGATTTATCGCGGTCTGCAAAGAAAAGCTTATGAACGCTTGATTTTAACCAATCCATTTCCAGAATTCACTGGACGAGT
CTGTCCGGCTCCTTGTGAAAAATCTTGTGCCGAGGCCTTAAACGGTGCAGGAGTAACCATTAAAGATAATGAGCGATTCT
TAGGTGATTTAGGAAATAATGAAGGTTGGGTAGCTGAAATTGGCAAAGCGGCAAAACCAACAGGCTATAAAGTAGCTATT
ATCGGTTCTGGACCTGCGGGTCTGTCAGCTGCATGGCGTCTTAATCAGTTGGGACATAGCGTTACCGTTTTTGAGAAGTC
AGACCGGTTGGGTGGCTTGTTGATGTATGGTATCCCAAATATGAAATTGGATAAAAAAGTCGTCCAAAAACGGATTGATT
TAATGACAGAACTTGGCGTGACTTTTGTCACAAATAGTGAAATTGGAAAAACAATCAGCTACGAAGAGCTATCAGTAAAT
TTTGATCGAATGATTCTGGCGATTGGCGCAGGAATTCCTCGTGATTTAAAAATTTCAGGTCGTGAATTGTCAGGGATTCG
TTTTGCAATGGATTTTTTGACAGAAACAACTAAAAAAGTGTTGGAGCATGGCGAAAAAAATATTGCCCAATCTCTTAAAG
GAAAAAAAGTTGTGGTCATTGGAGGTGGAGATACAGGAAATGACTGTATTGGGACGGCAGTACGCTTGGGTGCTGAATCG
GTTCAACAACTTGAAATTACACCGAGCCTACCAACTAATCGCCTAGAAAACAATCCCTGGCCTGAATGGCCAATGACCTT
AAGAGAGGGTTATGGGCAACAAGAAGCTCAATTTGCAGGAAATTCAGATTTGACGACTTATCAGATGACAGCCACAGCTT
TTAAAGCTGATGAAAATGGTCATCTAAAAGGCTTACAAGTGGCAAAAGTCGGTCCAGATTTCAGAGCAATTGAAGGTAGT
GAGCAATTGATTGAAGCAGATTTAGTCTTGCTTGCGATGGGATTTGTGGGTACGGACACAGGCTTGCTTGATAAATTTGG
TGTTCAAGAAATTTATGATGATTATCGAACAAACAATGATAAAGTGCTTGTCGCTGGAGATGCCAGACGTGGTCCGTCAT
TGGTTATCTGGGCAATCCGCGAAGGAAGAAAAACCGCTGAAATTGTTGACGAAGAGCTACTGAAAGTAGCCACGGCATAA

Upstream 100 bases:

>100_bases
ATCATCACATTAAAAATATTGAAGAAGATTTGGCTAAAACAGGATTGTCAGGCGAAGCACTTACAATGACAACCTTTAAA
AAAGTAACGGAGGTGAGCTA

Downstream 100 bases:

>100_bases
TTTATAACTCTGTCAGTAAAAAAGTTTCTGTCAGTAAAATGATGGGAACTTTTTTGTGTTTAAGTTTTTGACGATATGCT
ATACTTATAAATAATCTAAA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MADPNGFLNYPRNDNPYRELAERIKDFAELQVPLSTEERQKQAARCMHCDVPFCHQGIFYGGKRAVSGCPNDNHIPEWND
LIYRGLQRKAYERLILTNPFPEFTGRVCPAPCEKSCAEALNGAGVTIKDNERFLGDLGNNEGWVAEIGKAAKPTGYKVAI
IGSGPAGLSAAWRLNQLGHSVTVFEKSDRLGGLLMYGIPNMKLDKKVVQKRIDLMTELGVTFVTNSEIGKTISYEELSVN
FDRMILAIGAGIPRDLKISGRELSGIRFAMDFLTETTKKVLEHGEKNIAQSLKGKKVVVIGGGDTGNDCIGTAVRLGAES
VQQLEITPSLPTNRLENNPWPEWPMTLREGYGQQEAQFAGNSDLTTYQMTATAFKADENGHLKGLQVAKVGPDFRAIEGS
EQLIEADLVLLAMGFVGTDTGLLDKFGVQEIYDDYRTNNDKVLVAGDARRGPSLVIWAIREGRKTAEIVDEELLKVATA

Sequences:

>Translated_479_residues
MADPNGFLNYPRNDNPYRELAERIKDFAELQVPLSTEERQKQAARCMHCDVPFCHQGIFYGGKRAVSGCPNDNHIPEWND
LIYRGLQRKAYERLILTNPFPEFTGRVCPAPCEKSCAEALNGAGVTIKDNERFLGDLGNNEGWVAEIGKAAKPTGYKVAI
IGSGPAGLSAAWRLNQLGHSVTVFEKSDRLGGLLMYGIPNMKLDKKVVQKRIDLMTELGVTFVTNSEIGKTISYEELSVN
FDRMILAIGAGIPRDLKISGRELSGIRFAMDFLTETTKKVLEHGEKNIAQSLKGKKVVVIGGGDTGNDCIGTAVRLGAES
VQQLEITPSLPTNRLENNPWPEWPMTLREGYGQQEAQFAGNSDLTTYQMTATAFKADENGHLKGLQVAKVGPDFRAIEGS
EQLIEADLVLLAMGFVGTDTGLLDKFGVQEIYDDYRTNNDKVLVAGDARRGPSLVIWAIREGRKTAEIVDEELLKVATA
>Mature_478_residues
ADPNGFLNYPRNDNPYRELAERIKDFAELQVPLSTEERQKQAARCMHCDVPFCHQGIFYGGKRAVSGCPNDNHIPEWNDL
IYRGLQRKAYERLILTNPFPEFTGRVCPAPCEKSCAEALNGAGVTIKDNERFLGDLGNNEGWVAEIGKAAKPTGYKVAII
GSGPAGLSAAWRLNQLGHSVTVFEKSDRLGGLLMYGIPNMKLDKKVVQKRIDLMTELGVTFVTNSEIGKTISYEELSVNF
DRMILAIGAGIPRDLKISGRELSGIRFAMDFLTETTKKVLEHGEKNIAQSLKGKKVVVIGGGDTGNDCIGTAVRLGAESV
QQLEITPSLPTNRLENNPWPEWPMTLREGYGQQEAQFAGNSDLTTYQMTATAFKADENGHLKGLQVAKVGPDFRAIEGSE
QLIEADLVLLAMGFVGTDTGLLDKFGVQEIYDDYRTNNDKVLVAGDARRGPSLVIWAIREGRKTAEIVDEELLKVATA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=309, Percent_Identity=27.1844660194175, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789606, Length=478, Percent_Identity=32.8451882845188, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1788811, Length=479, Percent_Identity=32.9853862212944, Blast_Score=219, Evalue=5e-58,
Organism=Escherichia coli, GI87082180, Length=476, Percent_Identity=30.672268907563, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1788468, Length=442, Percent_Identity=27.6018099547511, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789243, Length=423, Percent_Identity=27.1867612293144, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17570289, Length=492, Percent_Identity=41.6666666666667, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71984108, Length=314, Percent_Identity=26.7515923566879, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=492, Percent_Identity=43.0894308943089, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI28574881, Length=486, Percent_Identity=44.8559670781893, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24665539, Length=486, Percent_Identity=44.8559670781893, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24665547, Length=486, Percent_Identity=44.8559670781893, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24665543, Length=486, Percent_Identity=44.8559670781893, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24640763, Length=321, Percent_Identity=27.1028037383178, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI18858217, Length=321, Percent_Identity=27.1028037383178, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52642; Mature: 52510

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPNGFLNYPRNDNPYRELAERIKDFAELQVPLSTEERQKQAARCMHCDVPFCHQGIFY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHEECCCCHHHCCCEE
GGKRAVSGCPNDNHIPEWNDLIYRGLQRKAYERLILTNPFPEFTGRVCPAPCEKSCAEAL
CCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHCCCCCCCCHHHHHHHHH
NGAGVTIKDNERFLGDLGNNEGWVAEIGKAAKPTGYKVAIIGSGPAGLSAAWRLNQLGHS
CCCCEEEECCCHHHHCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCE
VTVFEKSDRLGGLLMYGIPNMKLDKKVVQKRIDLMTELGVTFVTNSEIGKTISYEELSVN
EEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCEECHHHHCCC
FDRMILAIGAGIPRDLKISGRELSGIRFAMDFLTETTKKVLEHGEKNIAQSLKGKKVVVI
HHHEEEECCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEE
GGGDTGNDCIGTAVRLGAESVQQLEITPSLPTNRLENNPWPEWPMTLREGYGQQEAQFAG
ECCCCCCHHHHHHHHHCHHHHHEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHCCC
NSDLTTYQMTATAFKADENGHLKGLQVAKVGPDFRAIEGSEQLIEADLVLLAMGFVGTDT
CCCCEEEEEEEEEEECCCCCCCCEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHCCCC
GLLDKFGVQEIYDDYRTNNDKVLVAGDARRGPSLVIWAIREGRKTAEIVDEELLKVATA
CHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADPNGFLNYPRNDNPYRELAERIKDFAELQVPLSTEERQKQAARCMHCDVPFCHQGIFY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHEECCCCHHHCCCEE
GGKRAVSGCPNDNHIPEWNDLIYRGLQRKAYERLILTNPFPEFTGRVCPAPCEKSCAEAL
CCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHCCCCCCCCHHHHHHHHH
NGAGVTIKDNERFLGDLGNNEGWVAEIGKAAKPTGYKVAIIGSGPAGLSAAWRLNQLGHS
CCCCEEEECCCHHHHCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCE
VTVFEKSDRLGGLLMYGIPNMKLDKKVVQKRIDLMTELGVTFVTNSEIGKTISYEELSVN
EEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCEECHHHHCCC
FDRMILAIGAGIPRDLKISGRELSGIRFAMDFLTETTKKVLEHGEKNIAQSLKGKKVVVI
HHHEEEECCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEE
GGGDTGNDCIGTAVRLGAESVQQLEITPSLPTNRLENNPWPEWPMTLREGYGQQEAQFAG
ECCCCCCHHHHHHHHHCHHHHHEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHCCC
NSDLTTYQMTATAFKADENGHLKGLQVAKVGPDFRAIEGSEQLIEADLVLLAMGFVGTDT
CCCCEEEEEEEEEEECCCCCCCCEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHCCCC
GLLDKFGVQEIYDDYRTNNDKVLVAGDARRGPSLVIWAIREGRKTAEIVDEELLKVATA
CHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]