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The map label for this gene is prs [H]

Identifier: 124485597

GI number: 124485597

Start: 756359

End: 757204

Strand: Reverse

Name: prs [H]

Synonym: Mlab_0775

Alternate gene names: 124485597

Gene position: 757204-756359 (Counterclockwise)

Preceding gene: 124485605

Following gene: 124485596

Centisome position: 41.95

GC content: 54.14

Gene sequence:

>846_bases
ATGAAAGTAGTTTATACCGAAAAAAGCCAGGTTCTCGCGGCACGCACGGCGCAGAAGCTCGGCTGTCATCTATCTGAAGT
AAAATACACTACATTCCCGGATGGTGAGCAGTCCATACGCATAATGGATGATGATGACCAGATGATAATCATTGCAAGCA
CGGTCGATCCGGAGTCCACACTGCAGGCAATTCTCCTGCTCGATGCATGCGAGGGAAAAGAGACAACGCTTGTTCTGCCA
TATATGGGATACGCACGGCAGGACAAGAAGTTCAATGACGGCGAGCCGATCTCAGCACGGGCAATGGCACGGGCACTCTC
GACCGGCGCAGACAGGGTCTTTACCATCAATATCCATGATACCTCGATTCTCGGACATTTCCGCTGTCCTGCAAAGAATC
TGACGATTGCGCCGGAGATCGGAGCATACATCGGCACAATGGCTCTGGACAATCCGCTGATCCTTTCTCCCGATGAGGGT
GCATGGGAGTTTGCCAAAGGCGTGGCGGCGGTTGGAAAATGGGACTGTGATCATCTGGATAAGACGCGGCTCTCGGGTTC
GGAAGTTACTATGGCCCCAAAACACCTGGAAGCAAAAGGAAGAGACTGTATCATCGTGGATGATATCATCGCGACGGGCG
GTTCGATGGCAAAGGCCGCCGGAATGCTGCTCGAACAGGGGGCAACCTCGGTCCGGGCCGCGGGTGTTCACGGCGTTTTT
GCTTCCGGCGGATACATCAAACTTATGCAGGCCGGCCTTGCCGACATTGCATCCTCGGACACGATCGAGCGTGCCTCAAG
CAGGATCACGGCATCGGGTGTCATAGCAGACGCCATACGTAAATGA

Upstream 100 bases:

>100_bases
TGTTCCATATTGTGTTCCATACCTCAATTAAAGATGCTAATATTTGTACTTGATTATGCTTTAAGTAGTTTCAGTAAGAG
ATAGTTGTTAGAGTCTTATC

Downstream 100 bases:

>100_bases
AATATATTCTGGATTCATCCTATTTTTTCGGCGATTATACGCTTGACGGAGAGCTTTTTACCACTCCCGAGGTTGTCAGC
GAACTTAAGGATCTGGCATC

Product: hypothetical protein

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKVVYTEKSQVLAARTAQKLGCHLSEVKYTTFPDGEQSIRIMDDDDQMIIIASTVDPESTLQAILLLDACEGKETTLVLP
YMGYARQDKKFNDGEPISARAMARALSTGADRVFTINIHDTSILGHFRCPAKNLTIAPEIGAYIGTMALDNPLILSPDEG
AWEFAKGVAAVGKWDCDHLDKTRLSGSEVTMAPKHLEAKGRDCIIVDDIIATGGSMAKAAGMLLEQGATSVRAAGVHGVF
ASGGYIKLMQAGLADIASSDTIERASSRITASGVIADAIRK

Sequences:

>Translated_281_residues
MKVVYTEKSQVLAARTAQKLGCHLSEVKYTTFPDGEQSIRIMDDDDQMIIIASTVDPESTLQAILLLDACEGKETTLVLP
YMGYARQDKKFNDGEPISARAMARALSTGADRVFTINIHDTSILGHFRCPAKNLTIAPEIGAYIGTMALDNPLILSPDEG
AWEFAKGVAAVGKWDCDHLDKTRLSGSEVTMAPKHLEAKGRDCIIVDDIIATGGSMAKAAGMLLEQGATSVRAAGVHGVF
ASGGYIKLMQAGLADIASSDTIERASSRITASGVIADAIRK
>Mature_281_residues
MKVVYTEKSQVLAARTAQKLGCHLSEVKYTTFPDGEQSIRIMDDDDQMIIIASTVDPESTLQAILLLDACEGKETTLVLP
YMGYARQDKKFNDGEPISARAMARALSTGADRVFTINIHDTSILGHFRCPAKNLTIAPEIGAYIGTMALDNPLILSPDEG
AWEFAKGVAAVGKWDCDHLDKTRLSGSEVTMAPKHLEAKGRDCIIVDDIIATGGSMAKAAGMLLEQGATSVRAAGVHGVF
ASGGYIKLMQAGLADIASSDTIERASSRITASGVIADAIRK

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=290, Percent_Identity=30.6896551724138, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI84875539, Length=293, Percent_Identity=31.0580204778157, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI4506127, Length=299, Percent_Identity=29.7658862876254, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI28557709, Length=299, Percent_Identity=28.7625418060201, Blast_Score=104, Evalue=9e-23,
Organism=Escherichia coli, GI1787458, Length=299, Percent_Identity=32.1070234113712, Blast_Score=140, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI25149168, Length=288, Percent_Identity=28.4722222222222, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17554704, Length=288, Percent_Identity=28.4722222222222, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17554702, Length=288, Percent_Identity=28.4722222222222, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71989924, Length=288, Percent_Identity=28.4722222222222, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319403, Length=290, Percent_Identity=32.7586206896552, Blast_Score=128, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6320946, Length=289, Percent_Identity=31.8339100346021, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321776, Length=288, Percent_Identity=31.9444444444444, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355239, Length=263, Percent_Identity=29.277566539924, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI45551540, Length=284, Percent_Identity=28.169014084507, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 29947; Mature: 29947

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVYTEKSQVLAARTAQKLGCHLSEVKYTTFPDGEQSIRIMDDDDQMIIIASTVDPEST
CEEEEECCHHHHHHHHHHHHCCCHHHEEEEECCCCCCEEEEEECCCCEEEEEECCCCHHH
LQAILLLDACEGKETTLVLPYMGYARQDKKFNDGEPISARAMARALSTGADRVFTINIHD
HHEEHEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEC
TSILGHFRCPAKNLTIAPEIGAYIGTMALDNPLILSPDEGAWEFAKGVAAVGKWDCDHLD
CEEEEEEECCCCCCEECCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCH
KTRLSGSEVTMAPKHLEAKGRDCIIVDDIIATGGSMAKAAGMLLEQGATSVRAAGVHGVF
HHCCCCCEEEECCHHHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHCCCCCEEECCCCEEE
ASGGYIKLMQAGLADIASSDTIERASSRITASGVIADAIRK
CCCCEEEEHHHHHHHHHCCHHHHHHHCCEEHHHHHHHHHCC
>Mature Secondary Structure
MKVVYTEKSQVLAARTAQKLGCHLSEVKYTTFPDGEQSIRIMDDDDQMIIIASTVDPEST
CEEEEECCHHHHHHHHHHHHCCCHHHEEEEECCCCCCEEEEEECCCCEEEEEECCCCHHH
LQAILLLDACEGKETTLVLPYMGYARQDKKFNDGEPISARAMARALSTGADRVFTINIHD
HHEEHEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEC
TSILGHFRCPAKNLTIAPEIGAYIGTMALDNPLILSPDEGAWEFAKGVAAVGKWDCDHLD
CEEEEEEECCCCCCEECCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCH
KTRLSGSEVTMAPKHLEAKGRDCIIVDDIIATGGSMAKAAGMLLEQGATSVRAAGVHGVF
HHCCCCCEEEECCHHHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHCCCCCEEECCCCEEE
ASGGYIKLMQAGLADIASSDTIERASSRITASGVIADAIRK
CCCCEEEEHHHHHHHHHCCHHHHHHHCCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]