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The map label for this gene is gdhA [H]

Identifier: 124485529

GI number: 124485529

Start: 680054

End: 681304

Strand: Reverse

Name: gdhA [H]

Synonym: Mlab_0706

Alternate gene names: 124485529

Gene position: 681304-680054 (Counterclockwise)

Preceding gene: 124485530

Following gene: 124485527

Centisome position: 37.75

GC content: 53.24

Gene sequence:

>1251_bases
ATGTCGAAGGTAAACCCGTTTGAAATGGCCCAGCATCAGCTTATGGATTGTGCAAAAATCCTCAAGCTCGACCAGGGCGT
TGTTGATATCCTCATGCAACCGCAGAGACAGATTCAGGTATCTATCCCCGTCAAGATGGACGACGGCACCACCCGGGTTT
TCCAGGGATTCCGTGTACAGTACAACAATGCACTCGGACTCTATAAGGGAGGAATCCGGTATCACCCTGAAGAGACGATC
GACACCGTTCGTGCTCTCTCAGCATGGATGACCTGGAAATGCGCAGTACTGAACCTTCCGCTCGGCGGAGGAAAGGGCGG
TATCATCTGCAACCCGAAGGAAATGTCCAAGGGCGAACTTGAACGCATGAGCCGCGGTTATATCCGTGCAATCTGGAAGA
ACATCGGTCCCGACACTGATGTCCCGGCACCGGATGTATACACCGACGGTCAGATCATGGCATGGATGATGGATGAGTAC
TCCACCATTCAGGGCAAGAACCAGTTCGGTCTTCTGACTGGCAAACCACTCGTTATCGGCGGCTCACTCGGCCGCGGTGA
CTCCACCGCAAAAGGCGGTCTGTTCACCCTCCGTGAAGCAGCAAAAGAACTCAACATTGACCTGAAGACTGCAAAAGTCG
CAATCCTCGGATACGGAAACGCAGGTGCATTCGCATCCACGCTCGTCCAGGAAATGTTCGGCTCCAAAGTCGTCGCAGTC
ACCGATTCGAAAGGCGGTATCTACGATGAGAACGGACTTGACATCGCAGCAGTCGCAGCCCACAAGGCACAGACCAAATC
CGTCGTCGGCTACAAAGGTCTCAAGACCCTCACCAACGATGAAGTCATGGCACTCCCTGTTGATGTCATTGTCGCCGCAG
CACCCGATGAGGGAGCAATCAACGAGAAGGTCGCCCCGACCGTCAAGGCAAAGGTCATCTGCGAACTCGCAAACGGCCCG
ACCACTCCGGAAGGAGACGCAATCCTCTACAAGAACGGCGTTCACGTTATCCCCGACTTCCTGTGCAATGCAGGCGGAGT
AACTGTATCTTACTACGAGATGGTCCAGAATATGTATATGCACTACTGGACTCTCGACGACGTCTACGCAAAACTCGATG
CAGCAATGACCAAGTCCTATCACGAAGTCCTGAAAGCATCCAAAGAATACAAGATCAACATGCGTCAGGCAGCATACGTT
GTTGCAGTCTCCCGCGTAGTTGAAGGTATGAAAGTCCGCGGCTGGGTCTAA

Upstream 100 bases:

>100_bases
TCGGAAATAACACGATATGTTTTATATGTGAGAATCCCAACATTAATTGTCACAACGCAGTTGAACTCCAGAGTTCCGTG
CGATTGGCAGGAAACAAACC

Downstream 100 bases:

>100_bases
AACCCCCGCAAATTACCTTCACTATTTTTTTTATTCTTTTCTCACTCAACTGTAGTCAGAGAGTTGCCTCTGCAAAAACA
GGAATATCAGCCCAATTTAG

Product: glutamate dehydrogenase (NAD/NADP)

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSKVNPFEMAQHQLMDCAKILKLDQGVVDILMQPQRQIQVSIPVKMDDGTTRVFQGFRVQYNNALGLYKGGIRYHPEETI
DTVRALSAWMTWKCAVLNLPLGGGKGGIICNPKEMSKGELERMSRGYIRAIWKNIGPDTDVPAPDVYTDGQIMAWMMDEY
STIQGKNQFGLLTGKPLVIGGSLGRGDSTAKGGLFTLREAAKELNIDLKTAKVAILGYGNAGAFASTLVQEMFGSKVVAV
TDSKGGIYDENGLDIAAVAAHKAQTKSVVGYKGLKTLTNDEVMALPVDVIVAAAPDEGAINEKVAPTVKAKVICELANGP
TTPEGDAILYKNGVHVIPDFLCNAGGVTVSYYEMVQNMYMHYWTLDDVYAKLDAAMTKSYHEVLKASKEYKINMRQAAYV
VAVSRVVEGMKVRGWV

Sequences:

>Translated_416_residues
MSKVNPFEMAQHQLMDCAKILKLDQGVVDILMQPQRQIQVSIPVKMDDGTTRVFQGFRVQYNNALGLYKGGIRYHPEETI
DTVRALSAWMTWKCAVLNLPLGGGKGGIICNPKEMSKGELERMSRGYIRAIWKNIGPDTDVPAPDVYTDGQIMAWMMDEY
STIQGKNQFGLLTGKPLVIGGSLGRGDSTAKGGLFTLREAAKELNIDLKTAKVAILGYGNAGAFASTLVQEMFGSKVVAV
TDSKGGIYDENGLDIAAVAAHKAQTKSVVGYKGLKTLTNDEVMALPVDVIVAAAPDEGAINEKVAPTVKAKVICELANGP
TTPEGDAILYKNGVHVIPDFLCNAGGVTVSYYEMVQNMYMHYWTLDDVYAKLDAAMTKSYHEVLKASKEYKINMRQAAYV
VAVSRVVEGMKVRGWV
>Mature_415_residues
SKVNPFEMAQHQLMDCAKILKLDQGVVDILMQPQRQIQVSIPVKMDDGTTRVFQGFRVQYNNALGLYKGGIRYHPEETID
TVRALSAWMTWKCAVLNLPLGGGKGGIICNPKEMSKGELERMSRGYIRAIWKNIGPDTDVPAPDVYTDGQIMAWMMDEYS
TIQGKNQFGLLTGKPLVIGGSLGRGDSTAKGGLFTLREAAKELNIDLKTAKVAILGYGNAGAFASTLVQEMFGSKVVAVT
DSKGGIYDENGLDIAAVAAHKAQTKSVVGYKGLKTLTNDEVMALPVDVIVAAAPDEGAINEKVAPTVKAKVICELANGPT
TPEGDAILYKNGVHVIPDFLCNAGGVTVSYYEMVQNMYMHYWTLDDVYAKLDAAMTKSYHEVLKASKEYKINMRQAAYVV
AVSRVVEGMKVRGWV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=374, Percent_Identity=35.0267379679144, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI4885281, Length=361, Percent_Identity=35.4570637119114, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI1788059, Length=403, Percent_Identity=31.7617866004963, Blast_Score=184, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI17544676, Length=351, Percent_Identity=34.7578347578348, Blast_Score=190, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6319256, Length=398, Percent_Identity=31.6582914572864, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6324951, Length=393, Percent_Identity=31.8066157760814, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI45553475, Length=443, Percent_Identity=30.6997742663657, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI45549226, Length=357, Percent_Identity=34.733893557423, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24649283, Length=376, Percent_Identity=33.5106382978723, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24649460, Length=296, Percent_Identity=34.4594594594595, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 45291; Mature: 45160

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVNPFEMAQHQLMDCAKILKLDQGVVDILMQPQRQIQVSIPVKMDDGTTRVFQGFRVQ
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEEEECCCHHHHHHCEEEE
YNNALGLYKGGIRYHPEETIDTVRALSAWMTWKCAVLNLPLGGGKGGIICNPKEMSKGEL
ECCEEEEEECCCEECCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCEEECHHHCCHHHH
ERMSRGYIRAIWKNIGPDTDVPAPDVYTDGQIMAWMMDEYSTIQGKNQFGLLTGKPLVIG
HHHHHHHHHHHHHHCCCCCCCCCCCCEECCCEEEEEHHHHHHHCCCCCEEEEECCCEEEE
GSLGRGDSTAKGGLFTLREAAKELNIDLKTAKVAILGYGNAGAFASTLVQEMFGSKVVAV
CCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEECCCCHHHHHHHHHHHHCCEEEEE
TDSKGGIYDENGLDIAAVAAHKAQTKSVVGYKGLKTLTNDEVMALPVDVIVAAAPDEGAI
ECCCCCEECCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCC
NEKVAPTVKAKVICELANGPTTPEGDAILYKNGVHVIPDFLCNAGGVTVSYYEMVQNMYM
CCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCEEHHHHHHCCCCCEEHHHHHHHHHHH
HYWTLDDVYAKLDAAMTKSYHEVLKASKEYKINMRQAAYVVAVSRVVEGMKVRGWV
HEEEHHHHHHHHHHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SKVNPFEMAQHQLMDCAKILKLDQGVVDILMQPQRQIQVSIPVKMDDGTTRVFQGFRVQ
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEEEECCCHHHHHHCEEEE
YNNALGLYKGGIRYHPEETIDTVRALSAWMTWKCAVLNLPLGGGKGGIICNPKEMSKGEL
ECCEEEEEECCCEECCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCEEECHHHCCHHHH
ERMSRGYIRAIWKNIGPDTDVPAPDVYTDGQIMAWMMDEYSTIQGKNQFGLLTGKPLVIG
HHHHHHHHHHHHHHCCCCCCCCCCCCEECCCEEEEEHHHHHHHCCCCCEEEEECCCEEEE
GSLGRGDSTAKGGLFTLREAAKELNIDLKTAKVAILGYGNAGAFASTLVQEMFGSKVVAV
CCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEECCCCHHHHHHHHHHHHCCEEEEE
TDSKGGIYDENGLDIAAVAAHKAQTKSVVGYKGLKTLTNDEVMALPVDVIVAAAPDEGAI
ECCCCCEECCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCC
NEKVAPTVKAKVICELANGPTTPEGDAILYKNGVHVIPDFLCNAGGVTVSYYEMVQNMYM
CCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCEEHHHHHHCCCCCEEHHHHHHHHHHH
HYWTLDDVYAKLDAAMTKSYHEVLKASKEYKINMRQAAYVVAVSRVVEGMKVRGWV
HEEEHHHHHHHHHHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8135516 [H]