Click here to switch to the map view.

The map label for this gene is leuA2 [H]

Identifier: 124485428

GI number: 124485428

Start: 573235

End: 574803

Strand: Reverse

Name: leuA2 [H]

Synonym: Mlab_0603

Alternate gene names: 124485428

Gene position: 574803-573235 (Counterclockwise)

Preceding gene: 124485435

Following gene: 124485427

Centisome position: 31.85

GC content: 50.29

Gene sequence:

>1569_bases
ATGGGATCGGAGCAGTCACTGCGAAAGTGGCGGATTGCATTCTATTGCGATAAAAGTACAGTCAACACGAACAGAAGAGT
GACTATTTTAGACACCACACTTCGTGATGGTGAACAGACTCCCGGCGTGTCATTCACACTGGAGCAGAAAATTGAAATTG
CGCATCAGCTGTCCGATATCGGCGTTGATGTGATTGAAGCAGGCTTTCCGGCATCTTCGGATGTCGAATTTGAAACGGTC
AAGAGGATTTGCGCTGAAGAGGGTATCCGCCCCAAGATCTGCGGACTCGCACGCTCTGTCAAAGCGGATGTAGACCGTTG
TATCGAGGCCGGCGTTGATATGGTCCATGTGTTTATTCCCACATCTGAGATTCAGAGAACCTATACCATCAAAAAAAGTC
ATGCAGAAGTCCTGGCGATCACCCGGGAGATCATTACCTATGCACGTTCGAAGTGCGATTATGTGATGTTCTCGCCGATG
GATGCAACCAGAACTGAACCGTCGGAACTGATTGAAATCTGCAAAGCAGCAGATGAAGCCGGCACGACGATAATCAACAT
TCCCGACACAGTCGGTGTAAGCACCCCTTCGATGATAAAACCCCTCATCGCAATGATTCGCGAAAACGTCAAATGCAAAA
TCGATGTGCATTGTCACAACGATTTCGGCCTGGCGACCGCAAACACCATTGCAGCAGTTGAAGGAGGAGCTGACCAGATT
CAGGTCACCGTAAACGGTATCGGCGAACGGGCAGGTAACGCGGATTTAGCCCAGACGGTTATGATCCTGAAATCGATCTA
TGGAATCGAAACCAATATTCGGACCGAAAAACTGGTGGAGACTTCAAGAATGGTTTCCAGATTTTCACAGATTGCCGTTC
TGCCGATCCAGCCGGTCGTAGGCGAAAATGCATTTTCGCATGAAAGCGGCATCCATTCCCACGGCGTAATGGCTAACCCC
GGAACATTCGAGCCGGGAATCATGACGCCCGAAATGGTGGGTCACCGCCGGCGCTTAAAGCTTGGAAAGCATGTTGGAAA
ACATGCGGTCCGGCAGATGCTGGAGGACATAAACGTAAATCCGTCCGACCCCGAACTGGACATGATCGTCGCTAAAGTCA
AGGAGATCTCCGGACGCGGGCGAAAAGTAACCGAGTTCGATCTCTTCGAGATCGCAAAAATCATCACCGGCAGTCATAAC
GACAAAAAAATGATCGAGCTGGATGATATTTCGGTATTTACCGGGAGCCATGCGATTCCGACTGCAAGCGTACAGGCCGT
AGTCCACGGTGAAAACAAGATCTGTTCCAAAACGGGAGACGGACCGGTGGATGCAGCAATGAAGGCGCTTTTAGCGATCG
CACCTGGAAAAGTTCAGTTGAAGAGCTTTCAGATCGAGGCGATCTCCGGTGGAAGCGATGCGCTCGGATGCGTCACTATC
GAAGTCGAGGATGAAAAAGGCAGGATCTTTGATGCAGCCTCGTCAAACAGTGACATCGTGATTGCATCGGCTGAAGCGAT
GGTGAATGCCCTCAATGTCGTTTACCGCTCGGGTGGTTTCGACAGATAA

Upstream 100 bases:

>100_bases
CAGAAGTGCGCAGATATGGGGGTCTAACCCACTTTTTTTAGACACTAAACGAAAGTATTATTATGTATCGATGCATTGAT
ACTGGGTATGTCTCAGGAAG

Downstream 100 bases:

>100_bases
ACAATTAAATCGTAAGGAAGTTAATTTACTCAGTATCTATCAAAAAGGAAGGAAAAAAGATGATGGAAAAATATCATGAA
ACGGATGCGGACCTGAAAGA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MGSEQSLRKWRIAFYCDKSTVNTNRRVTILDTTLRDGEQTPGVSFTLEQKIEIAHQLSDIGVDVIEAGFPASSDVEFETV
KRICAEEGIRPKICGLARSVKADVDRCIEAGVDMVHVFIPTSEIQRTYTIKKSHAEVLAITREIITYARSKCDYVMFSPM
DATRTEPSELIEICKAADEAGTTIINIPDTVGVSTPSMIKPLIAMIRENVKCKIDVHCHNDFGLATANTIAAVEGGADQI
QVTVNGIGERAGNADLAQTVMILKSIYGIETNIRTEKLVETSRMVSRFSQIAVLPIQPVVGENAFSHESGIHSHGVMANP
GTFEPGIMTPEMVGHRRRLKLGKHVGKHAVRQMLEDINVNPSDPELDMIVAKVKEISGRGRKVTEFDLFEIAKIITGSHN
DKKMIELDDISVFTGSHAIPTASVQAVVHGENKICSKTGDGPVDAAMKALLAIAPGKVQLKSFQIEAISGGSDALGCVTI
EVEDEKGRIFDAASSNSDIVIASAEAMVNALNVVYRSGGFDR

Sequences:

>Translated_522_residues
MGSEQSLRKWRIAFYCDKSTVNTNRRVTILDTTLRDGEQTPGVSFTLEQKIEIAHQLSDIGVDVIEAGFPASSDVEFETV
KRICAEEGIRPKICGLARSVKADVDRCIEAGVDMVHVFIPTSEIQRTYTIKKSHAEVLAITREIITYARSKCDYVMFSPM
DATRTEPSELIEICKAADEAGTTIINIPDTVGVSTPSMIKPLIAMIRENVKCKIDVHCHNDFGLATANTIAAVEGGADQI
QVTVNGIGERAGNADLAQTVMILKSIYGIETNIRTEKLVETSRMVSRFSQIAVLPIQPVVGENAFSHESGIHSHGVMANP
GTFEPGIMTPEMVGHRRRLKLGKHVGKHAVRQMLEDINVNPSDPELDMIVAKVKEISGRGRKVTEFDLFEIAKIITGSHN
DKKMIELDDISVFTGSHAIPTASVQAVVHGENKICSKTGDGPVDAAMKALLAIAPGKVQLKSFQIEAISGGSDALGCVTI
EVEDEKGRIFDAASSNSDIVIASAEAMVNALNVVYRSGGFDR
>Mature_521_residues
GSEQSLRKWRIAFYCDKSTVNTNRRVTILDTTLRDGEQTPGVSFTLEQKIEIAHQLSDIGVDVIEAGFPASSDVEFETVK
RICAEEGIRPKICGLARSVKADVDRCIEAGVDMVHVFIPTSEIQRTYTIKKSHAEVLAITREIITYARSKCDYVMFSPMD
ATRTEPSELIEICKAADEAGTTIINIPDTVGVSTPSMIKPLIAMIRENVKCKIDVHCHNDFGLATANTIAAVEGGADQIQ
VTVNGIGERAGNADLAQTVMILKSIYGIETNIRTEKLVETSRMVSRFSQIAVLPIQPVVGENAFSHESGIHSHGVMANPG
TFEPGIMTPEMVGHRRRLKLGKHVGKHAVRQMLEDINVNPSDPELDMIVAKVKEISGRGRKVTEFDLFEIAKIITGSHND
KKMIELDDISVFTGSHAIPTASVQAVVHGENKICSKTGDGPVDAAMKALLAIAPGKVQLKSFQIEAISGGSDALGCVTIE
VEDEKGRIFDAASSNSDIVIASAEAMVNALNVVYRSGGFDR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=284, Percent_Identity=26.7605633802817, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI109150427, Length=286, Percent_Identity=26.9230769230769, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI109150422, Length=286, Percent_Identity=26.9230769230769, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1786261, Length=511, Percent_Identity=36.5949119373777, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1786548, Length=244, Percent_Identity=26.6393442622951, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320019, Length=374, Percent_Identity=32.620320855615, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6320071, Length=355, Percent_Identity=33.2394366197183, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324682, Length=312, Percent_Identity=28.8461538461538, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324225, Length=317, Percent_Identity=28.0757097791798, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24582381, Length=278, Percent_Identity=25.1798561151079, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56587; Mature: 56456

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSEQSLRKWRIAFYCDKSTVNTNRRVTILDTTLRDGEQTPGVSFTLEQKIEIAHQLSDI
CCCHHCHHEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHC
GVDVIEAGFPASSDVEFETVKRICAEEGIRPKICGLARSVKADVDRCIEAGVDMVHVFIP
CHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TSEIQRTYTIKKSHAEVLAITREIITYARSKCDYVMFSPMDATRTEPSELIEICKAADEA
HHHHHEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHC
GTTIINIPDTVGVSTPSMIKPLIAMIRENVKCKIDVHCHNDFGLATANTIAAVEGGADQI
CCEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEEECCCCCEE
QVTVNGIGERAGNADLAQTVMILKSIYGIETNIRTEKLVETSRMVSRFSQIAVLPIQPVV
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCC
GENAFSHESGIHSHGVMANPGTFEPGIMTPEMVGHRRRLKLGKHVGKHAVRQMLEDINVN
CCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCC
PSDPELDMIVAKVKEISGRGRKVTEFDLFEIAKIITGSHNDKKMIELDDISVFTGSHAIP
CCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCCCEEEEEECEEEEECCCCCC
TASVQAVVHGENKICSKTGDGPVDAAMKALLAIAPGKVQLKSFQIEAISGGSDALGCVTI
CCEEEEEEECCCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCEEEEEE
EVEDEKGRIFDAASSNSDIVIASAEAMVNALNVVYRSGGFDR
EEECCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GSEQSLRKWRIAFYCDKSTVNTNRRVTILDTTLRDGEQTPGVSFTLEQKIEIAHQLSDI
CCHHCHHEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHC
GVDVIEAGFPASSDVEFETVKRICAEEGIRPKICGLARSVKADVDRCIEAGVDMVHVFIP
CHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TSEIQRTYTIKKSHAEVLAITREIITYARSKCDYVMFSPMDATRTEPSELIEICKAADEA
HHHHHEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHC
GTTIINIPDTVGVSTPSMIKPLIAMIRENVKCKIDVHCHNDFGLATANTIAAVEGGADQI
CCEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEEECCCCCEE
QVTVNGIGERAGNADLAQTVMILKSIYGIETNIRTEKLVETSRMVSRFSQIAVLPIQPVV
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCC
GENAFSHESGIHSHGVMANPGTFEPGIMTPEMVGHRRRLKLGKHVGKHAVRQMLEDINVN
CCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCC
PSDPELDMIVAKVKEISGRGRKVTEFDLFEIAKIITGSHNDKKMIELDDISVFTGSHAIP
CCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCCCEEEEEECEEEEECCCCCC
TASVQAVVHGENKICSKTGDGPVDAAMKALLAIAPGKVQLKSFQIEAISGGSDALGCVTI
CCEEEEEEECCCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCEEEEEE
EVEDEKGRIFDAASSNSDIVIASAEAMVNALNVVYRSGGFDR
EEECCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]