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The map label for this gene is proS

Identifier: 124265710

GI number: 124265710

Start: 555190

End: 556935

Strand: Direct

Name: proS

Synonym: Mpe_A0517

Alternate gene names: 124265710

Gene position: 555190-556935 (Clockwise)

Preceding gene: 124265708

Following gene: 124265711

Centisome position: 13.73

GC content: 66.49

Gene sequence:

>1746_bases
ATGAAAGCCTCCCAGTTCTTCGTCTCCACCTTGAAAGAAGCACCGGCCGACGCCGAGATCACGAGCCACAAGCTGATGAT
GCGCGCCGGCATGATCAAGCGGCTCGGAGCCGGCATCTACAGCTACATGCCGATGGGGCTGCGCGTGATCCGCAAGGTCG
AGGCGATCATCCGCGAGGAAATGAACCGGGCGGGCGCCGTCGAACTGCTGATGCCCGTGGTGCAGCCGGCCGAGCTGTGG
CAGGAGACCGGTCGCTTCGAGAAGATGGGGCCCGAGCTGATGCGCGTGAAGGACCGCCACGACCGCGACTTCATCATCCA
GCCGACCAGCGAGGAGGTCGTCACCGACATCGCGCGGCAGGAACTGCGCAGCTACCGTCAGCTGCCGAAGAATTTCTATC
ACATCCAGACCAAGTTCCGTGACGAGCGACGGCCGCGCTTCGGCGTGATGCGCGGGCGCGAGTTCACCATGAAGGATGCC
TACTCCTTCGACCGTGATGAAGCGGCTGCCGGCAGGAGCTACGACGCGATGTACGCTGCCTACGGACGCATCTTCGACCG
TTTCGGCCTCAGCTACCGTGCGGTGGCGGCCGACACCGGCGCGATCGGTGGCGACCGCTCGCACGAGTTCCAGGTCATCG
CCGAGACGGGTGAGGATGCGATCGTCTACTGCCCGACCAGCGATTACGCGGCCAACATCGAGCTGGCCGAGTGTCTGCCG
CTCGCCGCGCAACGGCCGGTGCCGATTCAGCCGCTGACCAAGACGCCGACGCCCGGCAAGGCGACCTGCGCCGATGTGGC
GGCCCTGCTGAATCTGCCGCTGACGCAGACAGTGAAATCGCTGGTGCTGGCGACTGACGATCGCAATGACGGCGGCGACA
TCGTCAAGACCACCGTCTGGCTGCTGCTGGTGCGGGGCGATCATGAGCTCAATGAGGTCAAGGCCGGCAAGCTGCCAGGC
CTGAAGGCGGGCTTTCGCTTCGCGACCGTCGGTGAGATCGATGCGCACTTCGGCTGCAAGCCGGGCTACCTGGGACCGAT
CGGCCTGAAGCAGCCGGTGCGTGTGATCGCCGACCGGACGGTGGCGCACATGGGCGACTTCGTCTGCGGCGCCAACGACG
CCGACTTCCACTACACCGGCGTCAACTGGGGCCGCGACCTGCCCGAGCCCGACCTCGTGGCCGACATCCGCAATGCCGTC
GTGGGCGACCCTTCGCCGGACGGCAAGGGCGTGCTGGCGATCCAGCGTGGTATCGAGGTCGGCCACGTGTTCTACCTCGG
CACCAAGTACAGCGCCGCGATGAACGCCACCTACCTCGATGAGACCGGTAAGCCGCGGCTGATGGAGATGGGCTGCTACG
GCATCGGCGTCACGCGCATCCTCGGCGCGGCGATCGAGCAGCGCCACGACGCGCGAGGCATCGTGTGGCCGACCGCGATC
GCGCCCTTCGAGGTGGTCATCTGCCCGATCGGTTACGACCGTTCGGCCGAGGTCAGGCAGGCCGCCGACACTCTGCACGA
CGAACTGCAGGCACTCGGCATGGACTTGATGCTCGACGACCGCGGTGAGCGTCCCGGTGCCATGTTCGCCGATTGGGAAC
TGATCGGCATTCCGCAGCGCGTGGTGATCTCGGACCGCGGGCTGAAGGAGGGGCAGGTCGAGTTGCAGGGCCGGCAGGAG
GCCGAGGCCGGCAAGCTGGCAGTCGCCGAGGTGGTCGCGCAGTTGCGTGCGCGCTTGCGCGACTGA

Upstream 100 bases:

>100_bases
CGCGATGCAAGCCCGCTCCGCCCCAGGTTGCGGGGTCGGCCTGGCGGCATCGCTCCCAGCTCATCACGCAGCCTCCCTCT
CGCATGCCCCGGCCGACGCC

Downstream 100 bases:

>100_bases
TGCAGGGCACCCGCCGGCTCGTCCTGTTCGCTGGCCTGGGCCTCGCCGCAGGCCTGTCGCCCGCGCCGGCCCGTGCCGGA
TCGCAGATCGAGGAGCCTCT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MKASQFFVSTLKEAPADAEITSHKLMMRAGMIKRLGAGIYSYMPMGLRVIRKVEAIIREEMNRAGAVELLMPVVQPAELW
QETGRFEKMGPELMRVKDRHDRDFIIQPTSEEVVTDIARQELRSYRQLPKNFYHIQTKFRDERRPRFGVMRGREFTMKDA
YSFDRDEAAAGRSYDAMYAAYGRIFDRFGLSYRAVAADTGAIGGDRSHEFQVIAETGEDAIVYCPTSDYAANIELAECLP
LAAQRPVPIQPLTKTPTPGKATCADVAALLNLPLTQTVKSLVLATDDRNDGGDIVKTTVWLLLVRGDHELNEVKAGKLPG
LKAGFRFATVGEIDAHFGCKPGYLGPIGLKQPVRVIADRTVAHMGDFVCGANDADFHYTGVNWGRDLPEPDLVADIRNAV
VGDPSPDGKGVLAIQRGIEVGHVFYLGTKYSAAMNATYLDETGKPRLMEMGCYGIGVTRILGAAIEQRHDARGIVWPTAI
APFEVVICPIGYDRSAEVRQAADTLHDELQALGMDLMLDDRGERPGAMFADWELIGIPQRVVISDRGLKEGQVELQGRQE
AEAGKLAVAEVVAQLRARLRD

Sequences:

>Translated_581_residues
MKASQFFVSTLKEAPADAEITSHKLMMRAGMIKRLGAGIYSYMPMGLRVIRKVEAIIREEMNRAGAVELLMPVVQPAELW
QETGRFEKMGPELMRVKDRHDRDFIIQPTSEEVVTDIARQELRSYRQLPKNFYHIQTKFRDERRPRFGVMRGREFTMKDA
YSFDRDEAAAGRSYDAMYAAYGRIFDRFGLSYRAVAADTGAIGGDRSHEFQVIAETGEDAIVYCPTSDYAANIELAECLP
LAAQRPVPIQPLTKTPTPGKATCADVAALLNLPLTQTVKSLVLATDDRNDGGDIVKTTVWLLLVRGDHELNEVKAGKLPG
LKAGFRFATVGEIDAHFGCKPGYLGPIGLKQPVRVIADRTVAHMGDFVCGANDADFHYTGVNWGRDLPEPDLVADIRNAV
VGDPSPDGKGVLAIQRGIEVGHVFYLGTKYSAAMNATYLDETGKPRLMEMGCYGIGVTRILGAAIEQRHDARGIVWPTAI
APFEVVICPIGYDRSAEVRQAADTLHDELQALGMDLMLDDRGERPGAMFADWELIGIPQRVVISDRGLKEGQVELQGRQE
AEAGKLAVAEVVAQLRARLRD
>Mature_581_residues
MKASQFFVSTLKEAPADAEITSHKLMMRAGMIKRLGAGIYSYMPMGLRVIRKVEAIIREEMNRAGAVELLMPVVQPAELW
QETGRFEKMGPELMRVKDRHDRDFIIQPTSEEVVTDIARQELRSYRQLPKNFYHIQTKFRDERRPRFGVMRGREFTMKDA
YSFDRDEAAAGRSYDAMYAAYGRIFDRFGLSYRAVAADTGAIGGDRSHEFQVIAETGEDAIVYCPTSDYAANIELAECLP
LAAQRPVPIQPLTKTPTPGKATCADVAALLNLPLTQTVKSLVLATDDRNDGGDIVKTTVWLLLVRGDHELNEVKAGKLPG
LKAGFRFATVGEIDAHFGCKPGYLGPIGLKQPVRVIADRTVAHMGDFVCGANDADFHYTGVNWGRDLPEPDLVADIRNAV
VGDPSPDGKGVLAIQRGIEVGHVFYLGTKYSAAMNATYLDETGKPRLMEMGCYGIGVTRILGAAIEQRHDARGIVWPTAI
APFEVVICPIGYDRSAEVRQAADTLHDELQALGMDLMLDDRGERPGAMFADWELIGIPQRVVISDRGLKEGQVELQGRQE
AEAGKLAVAEVVAQLRARLRD

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=249, Percent_Identity=41.7670682730924, Blast_Score=210, Evalue=3e-54,
Organism=Escherichia coli, GI1786392, Length=580, Percent_Identity=54.8275862068965, Blast_Score=657, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=238, Percent_Identity=36.5546218487395, Blast_Score=161, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI193203271, Length=86, Percent_Identity=38.3720930232558, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=221, Percent_Identity=34.841628959276, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24656200, Length=244, Percent_Identity=37.2950819672131, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_METPP (A2SD40)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019714.1
- ProteinModelPortal:   A2SD40
- SMR:   A2SD40
- STRING:   A2SD40
- GeneID:   4787093
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0517
- NMPDR:   fig|279263.3.peg.2761
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   A2SD40
- ProtClustDB:   PRK09194
- BioCyc:   MPET420662:MPE_A0517-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64155; Mature: 64155

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASQFFVSTLKEAPADAEITSHKLMMRAGMIKRLGAGIYSYMPMGLRVIRKVEAIIREE
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAVELLMPVVQPAELWQETGRFEKMGPELMRVKDRHDRDFIIQPTSEEVVTDIARQ
HHHCCHHHHHHHHCCHHHHHHHCCCHHHCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
ELRSYRQLPKNFYHIQTKFRDERRPRFGVMRGREFTMKDAYSFDRDEAAAGRSYDAMYAA
HHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHCCCCHHHHHHH
YGRIFDRFGLSYRAVAADTGAIGGDRSHEFQVIAETGEDAIVYCPTSDYAANIELAECLP
HHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHH
LAAQRPVPIQPLTKTPTPGKATCADVAALLNLPLTQTVKSLVLATDDRNDGGDIVKTTVW
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCCHHHHEEEE
LLLVRGDHELNEVKAGKLPGLKAGFRFATVGEIDAHFGCKPGYLGPIGLKQPVRVIADRT
EEEEECCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VAHMGDFVCGANDADFHYTGVNWGRDLPEPDLVADIRNAVVGDPSPDGKGVLAIQRGIEV
HHHHCCHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCE
GHVFYLGTKYSAAMNATYLDETGKPRLMEMGCYGIGVTRILGAAIEQRHDARGIVWPTAI
EEEEEECCCHHHHCCCEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCC
APFEVVICPIGYDRSAEVRQAADTLHDELQALGMDLMLDDRGERPGAMFADWELIGIPQR
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECEEEECCCCE
VVISDRGLKEGQVELQGRQEAEAGKLAVAEVVAQLRARLRD
EEEECCCCCCCCEEECCCCCCHHCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKASQFFVSTLKEAPADAEITSHKLMMRAGMIKRLGAGIYSYMPMGLRVIRKVEAIIREE
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAVELLMPVVQPAELWQETGRFEKMGPELMRVKDRHDRDFIIQPTSEEVVTDIARQ
HHHCCHHHHHHHHCCHHHHHHHCCCHHHCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
ELRSYRQLPKNFYHIQTKFRDERRPRFGVMRGREFTMKDAYSFDRDEAAAGRSYDAMYAA
HHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHCCCCHHHHHHH
YGRIFDRFGLSYRAVAADTGAIGGDRSHEFQVIAETGEDAIVYCPTSDYAANIELAECLP
HHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHH
LAAQRPVPIQPLTKTPTPGKATCADVAALLNLPLTQTVKSLVLATDDRNDGGDIVKTTVW
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCCHHHHEEEE
LLLVRGDHELNEVKAGKLPGLKAGFRFATVGEIDAHFGCKPGYLGPIGLKQPVRVIADRT
EEEEECCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VAHMGDFVCGANDADFHYTGVNWGRDLPEPDLVADIRNAVVGDPSPDGKGVLAIQRGIEV
HHHHCCHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCE
GHVFYLGTKYSAAMNATYLDETGKPRLMEMGCYGIGVTRILGAAIEQRHDARGIVWPTAI
EEEEEECCCHHHHCCCEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCC
APFEVVICPIGYDRSAEVRQAADTLHDELQALGMDLMLDDRGERPGAMFADWELIGIPQR
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECEEEECCCCE
VVISDRGLKEGQVELQGRQEAEAGKLAVAEVVAQLRARLRD
EEEECCCCCCCCEEECCCCCCHHCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA