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The map label for this gene is ntrX [H]

Identifier: 124265468

GI number: 124265468

Start: 299019

End: 299708

Strand: Direct

Name: ntrX [H]

Synonym: Mpe_A0275

Alternate gene names: 124265468

Gene position: 299019-299708 (Clockwise)

Preceding gene: 124265467

Following gene: 124265469

Centisome position: 7.39

GC content: 69.13

Gene sequence:

>690_bases
ATGGCGACCATCCTTGTTGTAGACGACGAACTCGGCATCCGCGCGCTGCTGTCGGACATCCTGAGCGACGAGGGCCATAG
CGTCGAACTGGCCGAGAACGCCGCCGAGGCCCGGGCGTCGAGAGAGCGTGCGCGGCCCGATCTGGTGCTGCTCGACATCT
GGATGCCGGACGTCGATGGCGTCACGCTGCTCAAGGAATGGGGCAGCTGCAACCTGCTCACGATGCCGGTGATCATGATG
TCGGGCCACGGCACGATCGACACGGCCGTCGAGGCGATCAAGTTCGGCGCGATGGCCTTCCTCGAGAAGCCGATCACGCT
GCAGAAGCTGCTGCGGGCCGTCGAGCAGGGCCTGGCCAAGCCGGCCTCGCGGCCGGTGGCCACCGTGACGCCGCTGCGTC
CCGGCGAGCGCGGTGACGCGCTCGCCATCGATTCGCAGCACGGCGGGCTGCCCGGCGCCCAGCCGACCGCCCTCGGCCTG
CAACCCGAGCAGAGCTTCGAACTCGACCGGCCGCTGCGTGAAGCGCGCGACGCCTTCGAGAAGGCCTACTTCGAGTTCCA
CCTCGCACGCGAGAACGGCAGCATGACGCGCGTGGCCGAGAAGACCGGGCTGGAGCGCACCCACCTGTACCGCAAGCTCA
AGCAGCTCGGCGTGGACCTCAGCCGGGGCAAGAAGGGCGCGGCCGCCTGA

Upstream 100 bases:

>100_bases
CGGGGGGGCACAAGTTTCGCTATCATTTTCGAAATGGCTGCCCCCTCGGGGTCAGCCTTGAGGCCACGCCGACACGGCAT
GTGCCACGCACCGGGACCCC

Downstream 100 bases:

>100_bases
ACGGCCGGCGGTGCTGCTACACTGCGGCCCCTTGGCTCGGTAGCTCAGTTGGTAGAGCAGCGGATTGAAAATCCGCGTGT
CGGTGGTTCGATTCCGCCCC

Product: putative two component response regulator transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MATILVVDDELGIRALLSDILSDEGHSVELAENAAEARASRERARPDLVLLDIWMPDVDGVTLLKEWGSCNLLTMPVIMM
SGHGTIDTAVEAIKFGAMAFLEKPITLQKLLRAVEQGLAKPASRPVATVTPLRPGERGDALAIDSQHGGLPGAQPTALGL
QPEQSFELDRPLREARDAFEKAYFEFHLARENGSMTRVAEKTGLERTHLYRKLKQLGVDLSRGKKGAAA

Sequences:

>Translated_229_residues
MATILVVDDELGIRALLSDILSDEGHSVELAENAAEARASRERARPDLVLLDIWMPDVDGVTLLKEWGSCNLLTMPVIMM
SGHGTIDTAVEAIKFGAMAFLEKPITLQKLLRAVEQGLAKPASRPVATVTPLRPGERGDALAIDSQHGGLPGAQPTALGL
QPEQSFELDRPLREARDAFEKAYFEFHLARENGSMTRVAEKTGLERTHLYRKLKQLGVDLSRGKKGAAA
>Mature_228_residues
ATILVVDDELGIRALLSDILSDEGHSVELAENAAEARASRERARPDLVLLDIWMPDVDGVTLLKEWGSCNLLTMPVIMMS
GHGTIDTAVEAIKFGAMAFLEKPITLQKLLRAVEQGLAKPASRPVATVTPLRPGERGDALAIDSQHGGLPGAQPTALGLQ
PEQSFELDRPLREARDAFEKAYFEFHLARENGSMTRVAEKTGLERTHLYRKLKQLGVDLSRGKKGAAA

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=115, Percent_Identity=39.1304347826087, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790437, Length=120, Percent_Identity=36.6666666666667, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790299, Length=116, Percent_Identity=35.3448275862069, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788905, Length=121, Percent_Identity=38.0165289256198, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787375, Length=131, Percent_Identity=30.5343511450382, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 24902; Mature: 24771

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATILVVDDELGIRALLSDILSDEGHSVELAENAAEARASRERARPDLVLLDIWMPDVDG
CEEEEEEECCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
VTLLKEWGSCNLLTMPVIMMSGHGTIDTAVEAIKFGAMAFLEKPITLQKLLRAVEQGLAK
HHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
PASRPVATVTPLRPGERGDALAIDSQHGGLPGAQPTALGLQPEQSFELDRPLREARDAFE
CCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHH
KAYFEFHLARENGSMTRVAEKTGLERTHLYRKLKQLGVDLSRGKKGAAA
HHHHHEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCCCC
>Mature Secondary Structure 
ATILVVDDELGIRALLSDILSDEGHSVELAENAAEARASRERARPDLVLLDIWMPDVDG
EEEEEEECCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
VTLLKEWGSCNLLTMPVIMMSGHGTIDTAVEAIKFGAMAFLEKPITLQKLLRAVEQGLAK
HHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
PASRPVATVTPLRPGERGDALAIDSQHGGLPGAQPTALGLQPEQSFELDRPLREARDAFE
CCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHH
KAYFEFHLARENGSMTRVAEKTGLERTHLYRKLKQLGVDLSRGKKGAAA
HHHHHEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]