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The map label for this gene is rsmB [H]

Identifier: 124265465

GI number: 124265465

Start: 294607

End: 295956

Strand: Direct

Name: rsmB [H]

Synonym: Mpe_A0272

Alternate gene names: 124265465

Gene position: 294607-295956 (Clockwise)

Preceding gene: 124265464

Following gene: 124265466

Centisome position: 7.28

GC content: 72.44

Gene sequence:

>1350_bases
GTGAGCGCTCCTGTACCGCCGTCCCTCCCGCTGGCCACCCTGCTGGCGCACACCGCCGACGCGGTGGCTGCGGTGTGTGC
CGGGCGCTCGTTGACCGATGCGCTGGCGACCGTGCCGGCGGCGCCGCGCCCGGGCACGCAGGCGCTGGCGTTTCGCGTGC
TGCGCGGCCTCGGCCTCGCCCGGGCGCTGCGATGCCAACTCGTACCGAAGGCACCGCCGGCCTGGGTCGATGCACTGCTG
CTCAGTGCGCTCGCCCTGTCGCACCAGGATGCGGAGAGCGCCGACACCCCACCCTACGCGCCGCACACGCTGGTGAGCCA
GGCGGTGGAGGCGGCCAAGCAGCGCGCACGGGCACAGGCCGGCTTCGTCAACGCGGTGCTGCGGCGCCATCTGCGGGAGC
AGGCAACGCAGGCCGCGGCCGCGGCGAAGGATCCTGTCGCCGTATGGAACCATCCGCGCTGGTGGATCGAGACACTGCAG
CGCGACTGGCCAGGGCAGTGGGCCGAGCTGTTGCGTGCCAACGACCGGCGTGCGCCGATGACGTTGCGCGTCAATCAACG
GCAGCAGGACAGAGCCAGCTACCTGCTGCGCCTGGAGCAGGCGGGCATCGCTGCCCATGCCGTGACCCGGCCTGGCGCGA
TCGATGCCCAGGCCATCGAGCTCGCGACGCCCTGCGCTGTGTCCGCACTGCCGGGCTTTGCCGACGGCGCGGTGTCGGTG
CAGGACCTCGCGGCCCAGGTGGCGGCCCCCCTGCTGCTGGGCGCTGGCCTGGCGCCTGGCGCCCGGGTGCTCGACGCCTG
TGCGGCGCCCGGCGGCAAGACGGCTCACCTGCTCGAGCTGGCCGAGCTCGACCTGCTGGCCCTGGACGCCGACCCGCGGC
GGCTGGTGAAGGTGAGCGAGACGTTGCAGCGCCTCGGCCTGCGGGCACGCACCCAGGCCGCAGACGCACGCGACGTCGTC
GCCTGGTGGGACGGCCGGCCCTACGATGCCATCCTGCTCGACGCGCCCTGCAGCGCCTCGGGCATCGTGCGACGCCACCC
CGATGTGCGCTGGCTGCGCCGTGCCTCGGACCTCGATGCGCTGGCGTCGACCCAGGCTGAGCTGCTCGATGCGTTGTGGC
CGCTGTTGCGGCCCGGCGGGCGGCTGCTCTACGCCACCTGCTCGGTCTTCAAGGCCGAAGGCGCGCATCAGATCGACGCT
TTTTTGCAACGCGCACCCGATGCCATCGCGCAGCCTTCACCCGGCTACCTGCTGCCGGTGGTCGACAATCCCGGACAGGT
GAGCGAGGGGCCGCTGCGGCCTTCGACGGACGGTTTCTATTACGCGCTGCTCGAAAAGCGTTCGTCCTGA

Upstream 100 bases:

>100_bases
GGCCGGACTCCCAGATCGCCTCGTCGTTCGGTGACCGTGCCTGAGCGCCGCTGAGCCGGCCGGGCCTGGCGCAGCCGGCG
GCCGCCTACACTGCGCGGCC

Downstream 100 bases:

>100_bases
GCCGCACCGCAACGACCCATCATCCCAAACGCCTACCGCCACCGTGTTCACCGCCTCGGTTCGACGCTGGTTTCAGCCTG
CCGCAGGACGGTCGGCCGGG

Product: Sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSAPVPPSLPLATLLAHTADAVAAVCAGRSLTDALATVPAAPRPGTQALAFRVLRGLGLARALRCQLVPKAPPAWVDALL
LSALALSHQDAESADTPPYAPHTLVSQAVEAAKQRARAQAGFVNAVLRRHLREQATQAAAAAKDPVAVWNHPRWWIETLQ
RDWPGQWAELLRANDRRAPMTLRVNQRQQDRASYLLRLEQAGIAAHAVTRPGAIDAQAIELATPCAVSALPGFADGAVSV
QDLAAQVAAPLLLGAGLAPGARVLDACAAPGGKTAHLLELAELDLLALDADPRRLVKVSETLQRLGLRARTQAADARDVV
AWWDGRPYDAILLDAPCSASGIVRRHPDVRWLRRASDLDALASTQAELLDALWPLLRPGGRLLYATCSVFKAEGAHQIDA
FLQRAPDAIAQPSPGYLLPVVDNPGQVSEGPLRPSTDGFYYALLEKRSS

Sequences:

>Translated_449_residues
MSAPVPPSLPLATLLAHTADAVAAVCAGRSLTDALATVPAAPRPGTQALAFRVLRGLGLARALRCQLVPKAPPAWVDALL
LSALALSHQDAESADTPPYAPHTLVSQAVEAAKQRARAQAGFVNAVLRRHLREQATQAAAAAKDPVAVWNHPRWWIETLQ
RDWPGQWAELLRANDRRAPMTLRVNQRQQDRASYLLRLEQAGIAAHAVTRPGAIDAQAIELATPCAVSALPGFADGAVSV
QDLAAQVAAPLLLGAGLAPGARVLDACAAPGGKTAHLLELAELDLLALDADPRRLVKVSETLQRLGLRARTQAADARDVV
AWWDGRPYDAILLDAPCSASGIVRRHPDVRWLRRASDLDALASTQAELLDALWPLLRPGGRLLYATCSVFKAEGAHQIDA
FLQRAPDAIAQPSPGYLLPVVDNPGQVSEGPLRPSTDGFYYALLEKRSS
>Mature_448_residues
SAPVPPSLPLATLLAHTADAVAAVCAGRSLTDALATVPAAPRPGTQALAFRVLRGLGLARALRCQLVPKAPPAWVDALLL
SALALSHQDAESADTPPYAPHTLVSQAVEAAKQRARAQAGFVNAVLRRHLREQATQAAAAAKDPVAVWNHPRWWIETLQR
DWPGQWAELLRANDRRAPMTLRVNQRQQDRASYLLRLEQAGIAAHAVTRPGAIDAQAIELATPCAVSALPGFADGAVSVQ
DLAAQVAAPLLLGAGLAPGARVLDACAAPGGKTAHLLELAELDLLALDADPRRLVKVSETLQRLGLRARTQAADARDVVA
WWDGRPYDAILLDAPCSASGIVRRHPDVRWLRRASDLDALASTQAELLDALWPLLRPGGRLLYATCSVFKAEGAHQIDAF
LQRAPDAIAQPSPGYLLPVVDNPGQVSEGPLRPSTDGFYYALLEKRSS

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=278, Percent_Identity=28.4172661870504, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI76150623, Length=278, Percent_Identity=28.4172661870504, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI32698918, Length=168, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI40316918, Length=192, Percent_Identity=30.2083333333333, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI23199998, Length=236, Percent_Identity=33.4745762711864, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI270288816, Length=236, Percent_Identity=33.4745762711864, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI270288818, Length=236, Percent_Identity=33.4745762711864, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI8922322, Length=236, Percent_Identity=33.4745762711864, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI2367212, Length=351, Percent_Identity=43.3048433048433, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI87081985, Length=226, Percent_Identity=30.5309734513274, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=343, Percent_Identity=27.1137026239067, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324268, Length=281, Percent_Identity=26.6903914590747, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI22024126, Length=337, Percent_Identity=25.2225519287834, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920494, Length=175, Percent_Identity=33.1428571428571, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355201, Length=204, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 47819; Mature: 47688

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPVPPSLPLATLLAHTADAVAAVCAGRSLTDALATVPAAPRPGTQALAFRVLRGLGLA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
RALRCQLVPKAPPAWVDALLLSALALSHQDAESADTPPYAPHTLVSQAVEAAKQRARAQA
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GFVNAVLRRHLREQATQAAAAAKDPVAVWNHPRWWIETLQRDWPGQWAELLRANDRRAPM
HHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCE
TLRVNQRQQDRASYLLRLEQAGIAAHAVTRPGAIDAQAIELATPCAVSALPGFADGAVSV
EEEECCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHCCHHHHHCCCCCCCCCHH
QDLAAQVAAPLLLGAGLAPGARVLDACAAPGGKTAHLLELAELDLLALDADPRRLVKVSE
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCHHHHHHHHH
TLQRLGLRARTQAADARDVVAWWDGRPYDAILLDAPCSASGIVRRHPDVRWLRRASDLDA
HHHHHCCHHHHHCCCCCCEEEECCCCCCCEEEEECCCCCCCCHHCCCCHHHHHHHHHHHH
LASTQAELLDALWPLLRPGGRLLYATCSVFKAEGAHQIDAFLQRAPDAIAQPSPGYLLPV
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCHHHHHHHHHHCCHHHCCCCCCEEEEE
VDNPGQVSEGPLRPSTDGFYYALLEKRSS
ECCCCCCCCCCCCCCCCCCEEEEEHHCCC
>Mature Secondary Structure 
SAPVPPSLPLATLLAHTADAVAAVCAGRSLTDALATVPAAPRPGTQALAFRVLRGLGLA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
RALRCQLVPKAPPAWVDALLLSALALSHQDAESADTPPYAPHTLVSQAVEAAKQRARAQA
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GFVNAVLRRHLREQATQAAAAAKDPVAVWNHPRWWIETLQRDWPGQWAELLRANDRRAPM
HHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCE
TLRVNQRQQDRASYLLRLEQAGIAAHAVTRPGAIDAQAIELATPCAVSALPGFADGAVSV
EEEECCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHCCHHHHHCCCCCCCCCHH
QDLAAQVAAPLLLGAGLAPGARVLDACAAPGGKTAHLLELAELDLLALDADPRRLVKVSE
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCHHHHHHHHH
TLQRLGLRARTQAADARDVVAWWDGRPYDAILLDAPCSASGIVRRHPDVRWLRRASDLDA
HHHHHCCHHHHHCCCCCCEEEECCCCCCCEEEEECCCCCCCCHHCCCCHHHHHHHHHHHH
LASTQAELLDALWPLLRPGGRLLYATCSVFKAEGAHQIDAFLQRAPDAIAQPSPGYLLPV
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCHHHHHHHHHHCCHHHCCCCCCEEEEE
VDNPGQVSEGPLRPSTDGFYYALLEKRSS
ECCCCCCCCCCCCCCCCCCEEEEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]